Elderberry latent virus
Average proteome isoelectric point is 8.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4MJW8|A0A0B4MJW8_9TOMB Putative movement protein 2 OS=Elderberry latent virus OX=167018 GN=p9 PE=4 SV=1
MM1 pKa = 7.49 GLITQCIWFGAYY13 pKa = 9.78 VGFKK17 pKa = 8.16 VTKK20 pKa = 9.54 EE21 pKa = 3.69 LALLGPEE28 pKa = 4.25 LTSITLSCGFPEE40 pKa = 4.38 INPVLAAIGLPTVDD54 pKa = 5.11 YY55 pKa = 10.48 IPEE58 pKa = 4.08 VNQVDD63 pKa = 3.96 VLMATNDD70 pKa = 4.53 AILHH74 pKa = 5.43 TPDD77 pKa = 3.56 VEE79 pKa = 5.17 LEE81 pKa = 4.26 TEE83 pKa = 4.31 LEE85 pKa = 4.12 DD86 pKa = 5.03 SRR88 pKa = 11.84 VLTIAEE94 pKa = 4.44 PEE96 pKa = 4.2 TSRR99 pKa = 11.84 VTTVVKK105 pKa = 9.62 TRR107 pKa = 11.84 RR108 pKa = 11.84 PKK110 pKa = 10.02 NRR112 pKa = 11.84 RR113 pKa = 11.84 TFACVLAAEE122 pKa = 4.96 AKK124 pKa = 9.3 NHH126 pKa = 6.44 FGGMPRR132 pKa = 11.84 NSRR135 pKa = 11.84 ANEE138 pKa = 3.89 LSVMKK143 pKa = 10.28 YY144 pKa = 10.31 LVGKK148 pKa = 9.58 CQDD151 pKa = 3.45 HH152 pKa = 7.09 KK153 pKa = 10.97 LTALQTRR160 pKa = 11.84 EE161 pKa = 3.6 VAAQAFALTFTPDD174 pKa = 3.18 ANDD177 pKa = 3.08 KK178 pKa = 10.88 FIYY181 pKa = 10.05 TFLNSEE187 pKa = 4.0 DD188 pKa = 4.04 AYY190 pKa = 11.03 DD191 pKa = 3.75 RR192 pKa = 11.84 RR193 pKa = 11.84 CDD195 pKa = 3.67 YY196 pKa = 11.21 VKK198 pKa = 10.91 SQGVDD203 pKa = 3.6 PMWLRR208 pKa = 11.84 LLKK211 pKa = 10.59 RR212 pKa = 11.84 PFSRR216 pKa = 11.84 KK217 pKa = 6.31 EE218 pKa = 3.55 WKK220 pKa = 9.97 QVVCKK225 pKa = 10.29 FMGFGTRR232 pKa = 11.84 EE233 pKa = 3.73 AYY235 pKa = 9.43 TFIKK239 pKa = 10.56
Molecular weight: 26.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.905
IPC2_protein 7.205
IPC_protein 7.22
Toseland 6.81
ProMoST 7.907
Dawson 7.878
Bjellqvist 8.17
Wikipedia 7.79
Rodwell 7.878
Grimsley 6.81
Solomon 7.966
Lehninger 7.995
Nozaki 8.448
DTASelect 7.98
Thurlkill 8.039
EMBOSS 8.112
Sillero 8.375
Patrickios 4.418
IPC_peptide 7.966
IPC2_peptide 7.351
IPC2.peptide.svr19 7.427
Protein with the highest isoelectric point:
>tr|Q911J7|Q911J7_9TOMB Capsid protein OS=Elderberry latent virus OX=167018 GN=p37 PE=3 SV=1
MM1 pKa = 7.53 ANKK4 pKa = 10.35 SPVVVAAAGRR14 pKa = 11.84 GEE16 pKa = 4.08 LWAVKK21 pKa = 10.08 LSSLGWRR28 pKa = 11.84 SLSKK32 pKa = 10.46 AQKK35 pKa = 9.96 ASARR39 pKa = 11.84 AAGIGAVPQSAIIPRR54 pKa = 11.84 TTRR57 pKa = 11.84 LIAGNPTNSRR67 pKa = 11.84 RR68 pKa = 11.84 TRR70 pKa = 11.84 DD71 pKa = 3.4 APGNAQKK78 pKa = 11.43 SMTVFKK84 pKa = 11.25 SEE86 pKa = 4.33 FLGEE90 pKa = 3.78 LHH92 pKa = 7.27 ADD94 pKa = 3.36 NTIRR98 pKa = 11.84 SYY100 pKa = 11.62 KK101 pKa = 10.0 LDD103 pKa = 3.58 PRR105 pKa = 11.84 NVRR108 pKa = 11.84 TFPHH112 pKa = 6.46 ISDD115 pKa = 4.47 LARR118 pKa = 11.84 GFNKK122 pKa = 10.41 YY123 pKa = 10.29 KK124 pKa = 9.74 FTDD127 pKa = 3.29 VKK129 pKa = 10.68 VRR131 pKa = 11.84 YY132 pKa = 8.96 SSKK135 pKa = 10.68 VQDD138 pKa = 4.1 SEE140 pKa = 4.7 CGLTIAFSADD150 pKa = 3.16 SSDD153 pKa = 3.36 PAPRR157 pKa = 11.84 GKK159 pKa = 10.41 FDD161 pKa = 5.19 LYY163 pKa = 11.45 ALGSKK168 pKa = 10.39 VEE170 pKa = 4.36 GAAHH174 pKa = 6.93 KK175 pKa = 10.46 NLLYY179 pKa = 9.92 TVPVDD184 pKa = 2.89 KK185 pKa = 10.9 GQRR188 pKa = 11.84 FLRR191 pKa = 11.84 DD192 pKa = 3.47 GEE194 pKa = 4.16 GDD196 pKa = 3.31 NAKK199 pKa = 10.26 EE200 pKa = 3.57 VDD202 pKa = 3.25 AGVIYY207 pKa = 11.05 VLMDD211 pKa = 4.13 GAHH214 pKa = 6.63 DD215 pKa = 3.73 TRR217 pKa = 11.84 AGEE220 pKa = 4.23 LFLEE224 pKa = 4.64 VTVVLSQPTYY234 pKa = 8.64 NQRR237 pKa = 11.84 STQYY241 pKa = 10.77 ISGTEE246 pKa = 3.82 HH247 pKa = 7.08 RR248 pKa = 11.84 GGPNYY253 pKa = 10.51 VNITKK258 pKa = 10.23 SANTMTLTFQAAGTFLLCSYY278 pKa = 10.53 SSPLEE283 pKa = 3.85 KK284 pKa = 10.35 VGRR287 pKa = 11.84 LSLADD292 pKa = 3.89 ADD294 pKa = 3.97 QSQVDD299 pKa = 3.94 SANNSSNIIEE309 pKa = 4.38 AVVTTPGGSIVYY321 pKa = 8.25 YY322 pKa = 10.09 FKK324 pKa = 11.05 SRR326 pKa = 11.84 EE327 pKa = 3.93 QMSFRR332 pKa = 11.84 AYY334 pKa = 9.52 VGRR337 pKa = 11.84 MM338 pKa = 3.2
Molecular weight: 36.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.871
IPC2_protein 8.99
IPC_protein 8.96
Toseland 9.545
ProMoST 9.355
Dawson 9.838
Bjellqvist 9.545
Wikipedia 10.028
Rodwell 10.072
Grimsley 9.926
Solomon 9.867
Lehninger 9.823
Nozaki 9.575
DTASelect 9.531
Thurlkill 9.663
EMBOSS 9.97
Sillero 9.75
Patrickios 5.041
IPC_peptide 9.853
IPC2_peptide 8.141
IPC2.peptide.svr19 7.944
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1485
64
762
297.0
33.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.609 ± 1.205
1.751 ± 0.507
5.32 ± 0.459
5.32 ± 0.952
4.512 ± 0.267
6.801 ± 0.526
2.155 ± 0.32
4.444 ± 0.229
6.128 ± 0.441
9.091 ± 0.868
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.424 ± 0.359
3.838 ± 0.335
4.714 ± 0.407
3.03 ± 0.253
6.667 ± 0.605
7.003 ± 1.519
6.397 ± 0.752
7.879 ± 0.239
1.212 ± 0.379
3.636 ± 0.341
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here