Bacillus phage PfEFR-4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Hubeivirus; Bacillus virus PfEFR4

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1P7V8|A0A1B1P7V8_9CAUD Uncharacterized protein OS=Bacillus phage PfEFR-4 OX=1868598 PE=4 SV=1
MM1 pKa = 7.42SRR3 pKa = 11.84FKK5 pKa = 10.97FRR7 pKa = 11.84TWDD10 pKa = 3.12KK11 pKa = 10.07KK12 pKa = 10.91AEE14 pKa = 4.06VMEE17 pKa = 4.31QYY19 pKa = 10.65HH20 pKa = 5.92YY21 pKa = 11.16LQLSPIGQLYY31 pKa = 9.63HH32 pKa = 7.92DD33 pKa = 4.85GMNVTDD39 pKa = 4.44NYY41 pKa = 10.93EE42 pKa = 3.73IMQYY46 pKa = 10.09TGLKK50 pKa = 10.05DD51 pKa = 3.95KK52 pKa = 10.8NDD54 pKa = 3.41KK55 pKa = 10.41EE56 pKa = 4.33IYY58 pKa = 9.83EE59 pKa = 4.24GDD61 pKa = 3.02IVRR64 pKa = 11.84YY65 pKa = 9.86LDD67 pKa = 3.56GDD69 pKa = 3.6EE70 pKa = 4.31WSTEE74 pKa = 3.66SGYY77 pKa = 11.15DD78 pKa = 3.45CEE80 pKa = 5.4EE81 pKa = 4.11FDD83 pKa = 3.38NHH85 pKa = 5.89GVIFFDD91 pKa = 4.41EE92 pKa = 4.25EE93 pKa = 3.97CGRR96 pKa = 11.84YY97 pKa = 9.61DD98 pKa = 3.35VTNKK102 pKa = 10.18QGISYY107 pKa = 10.79DD108 pKa = 3.73DD109 pKa = 4.99LFDD112 pKa = 4.81CGVDD116 pKa = 4.28FEE118 pKa = 5.55IIGNIYY124 pKa = 9.85EE125 pKa = 4.17NPEE128 pKa = 4.31LIKK131 pKa = 11.08NN132 pKa = 3.79

Molecular weight: 15.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1P7Y2|A0A1B1P7Y2_9CAUD Site-specific integrase OS=Bacillus phage PfEFR-4 OX=1868598 PE=3 SV=1
MM1 pKa = 7.2EE2 pKa = 5.51RR3 pKa = 11.84MRR5 pKa = 11.84HH6 pKa = 3.88TRR8 pKa = 11.84NRR10 pKa = 11.84QMTKK14 pKa = 9.4IGEE17 pKa = 4.17EE18 pKa = 3.7NFMGMKK24 pKa = 9.74NIKK27 pKa = 9.73ISTIRR32 pKa = 11.84KK33 pKa = 9.08FDD35 pKa = 3.56GEE37 pKa = 4.45FNNRR41 pKa = 11.84LDD43 pKa = 3.56PTFRR47 pKa = 11.84WHH49 pKa = 7.54RR50 pKa = 11.84DD51 pKa = 3.27YY52 pKa = 11.54HH53 pKa = 6.99GMNIVSFNRR62 pKa = 11.84KK63 pKa = 9.3GEE65 pKa = 4.0AFKK68 pKa = 11.02TNVIKK73 pKa = 10.88VRR75 pKa = 11.84NN76 pKa = 3.71

Molecular weight: 9.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

12068

34

1671

188.6

21.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.215 ± 0.598

0.746 ± 0.147

5.386 ± 0.266

8.494 ± 0.284

4.035 ± 0.205

6.231 ± 0.497

1.55 ± 0.166

6.969 ± 0.305

9.364 ± 0.387

7.897 ± 0.308

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.058 ± 0.179

5.37 ± 0.225

2.776 ± 0.245

4.723 ± 0.428

4.881 ± 0.299

5.535 ± 0.228

5.709 ± 0.273

6.198 ± 0.228

1.334 ± 0.144

3.53 ± 0.27
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski