Amino acid dipepetide frequency for Bacillus phage PfEFR-4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.558AlaAla: 4.558 ± 0.757
0.663AlaCys: 0.663 ± 0.232
2.32AlaAsp: 2.32 ± 0.496
5.221AlaGlu: 5.221 ± 0.651
2.072AlaPhe: 2.072 ± 0.48
3.978AlaGly: 3.978 ± 0.984
0.829AlaHis: 0.829 ± 0.301
5.055AlaIle: 5.055 ± 0.562
5.552AlaLys: 5.552 ± 0.462
6.381AlaLeu: 6.381 ± 1.06
1.657AlaMet: 1.657 ± 0.351
3.563AlaAsn: 3.563 ± 0.73
1.409AlaPro: 1.409 ± 0.463
2.155AlaGln: 2.155 ± 0.439
2.32AlaArg: 2.32 ± 0.527
2.983AlaSer: 2.983 ± 0.638
3.978AlaThr: 3.978 ± 0.712
4.309AlaVal: 4.309 ± 0.891
1.409AlaTrp: 1.409 ± 0.442
1.74AlaTyr: 1.74 ± 0.404
0.0AlaXaa: 0.0 ± 0.0
Cys
0.497CysAla: 0.497 ± 0.207
0.0CysCys: 0.0 ± 0.0
0.414CysAsp: 0.414 ± 0.179
1.077CysGlu: 1.077 ± 0.308
0.58CysPhe: 0.58 ± 0.256
0.414CysGly: 0.414 ± 0.227
0.166CysHis: 0.166 ± 0.119
0.249CysIle: 0.249 ± 0.16
0.331CysLys: 0.331 ± 0.196
0.746CysLeu: 0.746 ± 0.304
0.497CysMet: 0.497 ± 0.251
0.414CysAsn: 0.414 ± 0.174
0.497CysPro: 0.497 ± 0.232
0.249CysGln: 0.249 ± 0.147
0.166CysArg: 0.166 ± 0.123
0.331CysSer: 0.331 ± 0.161
0.249CysThr: 0.249 ± 0.136
0.414CysVal: 0.414 ± 0.205
0.0CysTrp: 0.0 ± 0.0
0.166CysTyr: 0.166 ± 0.108
0.0CysXaa: 0.0 ± 0.0
Asp
3.729AspAla: 3.729 ± 0.586
0.331AspCys: 0.331 ± 0.24
3.563AspAsp: 3.563 ± 0.727
4.392AspGlu: 4.392 ± 0.728
1.823AspPhe: 1.823 ± 0.358
3.481AspGly: 3.481 ± 0.564
1.077AspHis: 1.077 ± 0.235
4.061AspIle: 4.061 ± 0.555
5.138AspLys: 5.138 ± 0.647
4.724AspLeu: 4.724 ± 0.69
1.492AspMet: 1.492 ± 0.339
2.569AspAsn: 2.569 ± 0.538
1.492AspPro: 1.492 ± 0.333
1.575AspGln: 1.575 ± 0.286
1.657AspArg: 1.657 ± 0.38
2.9AspSer: 2.9 ± 0.509
3.232AspThr: 3.232 ± 0.541
3.729AspVal: 3.729 ± 0.457
1.077AspTrp: 1.077 ± 0.302
1.823AspTyr: 1.823 ± 0.346
0.0AspXaa: 0.0 ± 0.0
Glu
4.806GluAla: 4.806 ± 0.69
0.912GluCys: 0.912 ± 0.347
2.9GluAsp: 2.9 ± 0.409
6.298GluGlu: 6.298 ± 0.82
3.149GluPhe: 3.149 ± 0.543
5.635GluGly: 5.635 ± 0.701
1.243GluHis: 1.243 ± 0.278
7.127GluIle: 7.127 ± 0.683
7.044GluLys: 7.044 ± 0.683
9.116GluLeu: 9.116 ± 0.849
3.232GluMet: 3.232 ± 0.687
3.812GluAsn: 3.812 ± 0.618
1.575GluPro: 1.575 ± 0.372
3.895GluGln: 3.895 ± 0.537
4.724GluArg: 4.724 ± 0.789
5.221GluSer: 5.221 ± 0.761
4.392GluThr: 4.392 ± 0.72
6.05GluVal: 6.05 ± 0.851
1.077GluTrp: 1.077 ± 0.306
2.735GluTyr: 2.735 ± 0.466
0.0GluXaa: 0.0 ± 0.0
Phe
1.657PheAla: 1.657 ± 0.36
0.497PheCys: 0.497 ± 0.196
2.569PheAsp: 2.569 ± 0.518
2.652PheGlu: 2.652 ± 0.337
1.243PhePhe: 1.243 ± 0.34
2.403PheGly: 2.403 ± 0.351
0.912PheHis: 0.912 ± 0.286
2.983PheIle: 2.983 ± 0.561
4.724PheLys: 4.724 ± 0.638
3.149PheLeu: 3.149 ± 0.456
1.16PheMet: 1.16 ± 0.345
2.652PheAsn: 2.652 ± 0.369
1.077PhePro: 1.077 ± 0.376
1.823PheGln: 1.823 ± 0.386
2.652PheArg: 2.652 ± 0.506
1.823PheSer: 1.823 ± 0.379
2.238PheThr: 2.238 ± 0.472
2.072PheVal: 2.072 ± 0.464
0.166PheTrp: 0.166 ± 0.098
1.906PheTyr: 1.906 ± 0.326
0.0PheXaa: 0.0 ± 0.0
Gly
3.481GlyAla: 3.481 ± 1.111
0.249GlyCys: 0.249 ± 0.165
3.812GlyAsp: 3.812 ± 0.474
4.226GlyGlu: 4.226 ± 0.682
3.398GlyPhe: 3.398 ± 0.658
3.066GlyGly: 3.066 ± 0.621
0.58GlyHis: 0.58 ± 0.141
5.469GlyIle: 5.469 ± 0.767
6.132GlyLys: 6.132 ± 0.684
4.806GlyLeu: 4.806 ± 0.664
1.823GlyMet: 1.823 ± 0.339
3.315GlyAsn: 3.315 ± 0.459
0.497GlyPro: 0.497 ± 0.172
2.652GlyGln: 2.652 ± 0.78
2.569GlyArg: 2.569 ± 0.425
4.392GlySer: 4.392 ± 0.748
2.569GlyThr: 2.569 ± 0.44
4.309GlyVal: 4.309 ± 0.573
1.657GlyTrp: 1.657 ± 0.316
3.232GlyTyr: 3.232 ± 0.543
0.0GlyXaa: 0.0 ± 0.0
His
0.58HisAla: 0.58 ± 0.169
0.166HisCys: 0.166 ± 0.121
0.829HisAsp: 0.829 ± 0.257
0.746HisGlu: 0.746 ± 0.287
1.16HisPhe: 1.16 ± 0.267
0.829HisGly: 0.829 ± 0.259
0.331HisHis: 0.331 ± 0.163
0.994HisIle: 0.994 ± 0.232
1.409HisLys: 1.409 ± 0.329
1.492HisLeu: 1.492 ± 0.417
0.249HisMet: 0.249 ± 0.129
0.58HisAsn: 0.58 ± 0.208
0.497HisPro: 0.497 ± 0.253
0.663HisGln: 0.663 ± 0.199
0.58HisArg: 0.58 ± 0.19
1.326HisSer: 1.326 ± 0.371
0.994HisThr: 0.994 ± 0.301
1.077HisVal: 1.077 ± 0.297
0.083HisTrp: 0.083 ± 0.102
0.912HisTyr: 0.912 ± 0.231
0.0HisXaa: 0.0 ± 0.0
Ile
4.806IleAla: 4.806 ± 0.521
0.58IleCys: 0.58 ± 0.193
4.972IleAsp: 4.972 ± 0.598
7.044IleGlu: 7.044 ± 0.62
2.486IlePhe: 2.486 ± 0.496
3.481IleGly: 3.481 ± 0.467
0.912IleHis: 0.912 ± 0.306
3.895IleIle: 3.895 ± 0.483
6.63IleLys: 6.63 ± 0.719
4.475IleLeu: 4.475 ± 0.556
1.326IleMet: 1.326 ± 0.266
3.812IleAsn: 3.812 ± 0.666
2.403IlePro: 2.403 ± 0.333
3.481IleGln: 3.481 ± 0.594
3.646IleArg: 3.646 ± 0.533
4.972IleSer: 4.972 ± 0.628
4.558IleThr: 4.558 ± 0.492
3.066IleVal: 3.066 ± 0.517
1.16IleTrp: 1.16 ± 0.382
1.989IleTyr: 1.989 ± 0.453
0.0IleXaa: 0.0 ± 0.0
Lys
6.547LysAla: 6.547 ± 0.777
0.746LysCys: 0.746 ± 0.226
4.889LysAsp: 4.889 ± 0.641
9.447LysGlu: 9.447 ± 0.984
3.315LysPhe: 3.315 ± 0.475
5.884LysGly: 5.884 ± 0.498
1.326LysHis: 1.326 ± 0.373
6.132LysIle: 6.132 ± 0.612
7.541LysLys: 7.541 ± 1.071
6.464LysLeu: 6.464 ± 0.716
3.895LysMet: 3.895 ± 0.563
5.387LysAsn: 5.387 ± 0.705
2.155LysPro: 2.155 ± 0.329
5.635LysGln: 5.635 ± 0.621
3.978LysArg: 3.978 ± 0.512
4.475LysSer: 4.475 ± 0.483
5.635LysThr: 5.635 ± 0.701
5.801LysVal: 5.801 ± 0.81
1.243LysTrp: 1.243 ± 0.334
3.149LysTyr: 3.149 ± 0.493
0.0LysXaa: 0.0 ± 0.0
Leu
4.309LeuAla: 4.309 ± 0.854
0.663LeuCys: 0.663 ± 0.268
4.475LeuAsp: 4.475 ± 0.543
6.464LeuGlu: 6.464 ± 0.769
2.486LeuPhe: 2.486 ± 0.536
5.387LeuGly: 5.387 ± 0.63
0.994LeuHis: 0.994 ± 0.278
4.806LeuIle: 4.806 ± 0.645
8.95LeuLys: 8.95 ± 0.886
5.635LeuLeu: 5.635 ± 0.759
1.74LeuMet: 1.74 ± 0.369
5.055LeuAsn: 5.055 ± 0.58
2.652LeuPro: 2.652 ± 0.586
4.309LeuGln: 4.309 ± 0.878
4.309LeuArg: 4.309 ± 0.92
4.889LeuSer: 4.889 ± 0.773
4.641LeuThr: 4.641 ± 0.59
3.646LeuVal: 3.646 ± 0.539
0.663LeuTrp: 0.663 ± 0.227
2.9LeuTyr: 2.9 ± 0.396
0.0LeuXaa: 0.0 ± 0.0
Met
2.32MetAla: 2.32 ± 0.497
0.249MetCys: 0.249 ± 0.126
1.823MetAsp: 1.823 ± 0.399
2.486MetGlu: 2.486 ± 0.468
0.994MetPhe: 0.994 ± 0.286
1.657MetGly: 1.657 ± 0.349
0.0MetHis: 0.0 ± 0.0
2.238MetIle: 2.238 ± 0.437
3.729MetLys: 3.729 ± 0.505
2.155MetLeu: 2.155 ± 0.524
0.994MetMet: 0.994 ± 0.279
2.072MetAsn: 2.072 ± 0.467
0.663MetPro: 0.663 ± 0.286
1.409MetGln: 1.409 ± 0.352
2.238MetArg: 2.238 ± 0.405
1.409MetSer: 1.409 ± 0.333
1.989MetThr: 1.989 ± 0.39
1.243MetVal: 1.243 ± 0.333
0.414MetTrp: 0.414 ± 0.197
0.497MetTyr: 0.497 ± 0.198
0.0MetXaa: 0.0 ± 0.0
Asn
3.563AsnAla: 3.563 ± 0.598
0.083AsnCys: 0.083 ± 0.087
2.403AsnAsp: 2.403 ± 0.391
5.055AsnGlu: 5.055 ± 0.578
1.409AsnPhe: 1.409 ± 0.293
4.641AsnGly: 4.641 ± 0.699
1.16AsnHis: 1.16 ± 0.384
2.9AsnIle: 2.9 ± 0.607
5.635AsnLys: 5.635 ± 0.573
3.315AsnLeu: 3.315 ± 0.491
2.072AsnMet: 2.072 ± 0.354
2.403AsnAsn: 2.403 ± 0.519
2.072AsnPro: 2.072 ± 0.372
2.818AsnGln: 2.818 ± 0.596
2.818AsnArg: 2.818 ± 0.569
2.155AsnSer: 2.155 ± 0.437
3.315AsnThr: 3.315 ± 0.476
3.563AsnVal: 3.563 ± 0.558
0.58AsnTrp: 0.58 ± 0.241
1.74AsnTyr: 1.74 ± 0.416
0.0AsnXaa: 0.0 ± 0.0
Pro
0.994ProAla: 0.994 ± 0.26
0.0ProCys: 0.0 ± 0.0
1.409ProAsp: 1.409 ± 0.287
2.403ProGlu: 2.403 ± 0.416
1.16ProPhe: 1.16 ± 0.292
1.243ProGly: 1.243 ± 0.266
0.994ProHis: 0.994 ± 0.352
2.155ProIle: 2.155 ± 0.518
2.818ProLys: 2.818 ± 0.603
1.74ProLeu: 1.74 ± 0.445
0.746ProMet: 0.746 ± 0.318
0.58ProAsn: 0.58 ± 0.182
1.575ProPro: 1.575 ± 0.446
1.077ProGln: 1.077 ± 0.35
0.994ProArg: 0.994 ± 0.29
2.818ProSer: 2.818 ± 0.587
1.575ProThr: 1.575 ± 0.497
2.32ProVal: 2.32 ± 0.554
0.166ProTrp: 0.166 ± 0.16
0.994ProTyr: 0.994 ± 0.29
0.0ProXaa: 0.0 ± 0.0
Gln
3.646GlnAla: 3.646 ± 0.762
0.166GlnCys: 0.166 ± 0.123
1.409GlnAsp: 1.409 ± 0.26
3.646GlnGlu: 3.646 ± 0.539
2.072GlnPhe: 2.072 ± 0.428
3.149GlnGly: 3.149 ± 0.816
0.829GlnHis: 0.829 ± 0.291
1.74GlnIle: 1.74 ± 0.318
4.558GlnLys: 4.558 ± 0.593
4.144GlnLeu: 4.144 ± 0.667
1.74GlnMet: 1.74 ± 0.356
2.569GlnAsn: 2.569 ± 0.728
1.657GlnPro: 1.657 ± 0.405
2.983GlnGln: 2.983 ± 0.531
2.818GlnArg: 2.818 ± 0.443
1.906GlnSer: 1.906 ± 0.362
2.403GlnThr: 2.403 ± 0.532
2.818GlnVal: 2.818 ± 0.478
0.994GlnTrp: 0.994 ± 0.263
1.989GlnTyr: 1.989 ± 0.403
0.0GlnXaa: 0.0 ± 0.0
Arg
2.238ArgAla: 2.238 ± 0.484
0.331ArgCys: 0.331 ± 0.196
3.729ArgAsp: 3.729 ± 0.52
4.061ArgGlu: 4.061 ± 0.644
2.983ArgPhe: 2.983 ± 0.382
2.9ArgGly: 2.9 ± 0.501
0.829ArgHis: 0.829 ± 0.297
4.226ArgIle: 4.226 ± 0.7
5.055ArgLys: 5.055 ± 0.981
3.646ArgLeu: 3.646 ± 0.551
1.243ArgMet: 1.243 ± 0.275
2.9ArgAsn: 2.9 ± 0.558
0.912ArgPro: 0.912 ± 0.303
1.906ArgGln: 1.906 ± 0.397
2.072ArgArg: 2.072 ± 0.414
1.906ArgSer: 1.906 ± 0.381
2.072ArgThr: 2.072 ± 0.364
3.315ArgVal: 3.315 ± 0.512
0.829ArgTrp: 0.829 ± 0.265
1.657ArgTyr: 1.657 ± 0.381
0.0ArgXaa: 0.0 ± 0.0
Ser
3.978SerAla: 3.978 ± 0.599
0.331SerCys: 0.331 ± 0.168
2.652SerAsp: 2.652 ± 0.444
4.144SerGlu: 4.144 ± 0.428
2.072SerPhe: 2.072 ± 0.322
4.144SerGly: 4.144 ± 0.645
0.414SerHis: 0.414 ± 0.152
3.812SerIle: 3.812 ± 0.573
4.641SerLys: 4.641 ± 0.645
4.226SerLeu: 4.226 ± 0.452
2.238SerMet: 2.238 ± 0.478
2.9SerAsn: 2.9 ± 0.525
1.492SerPro: 1.492 ± 0.342
1.989SerGln: 1.989 ± 0.48
2.983SerArg: 2.983 ± 0.496
3.232SerSer: 3.232 ± 0.654
3.481SerThr: 3.481 ± 0.507
4.061SerVal: 4.061 ± 0.754
0.58SerTrp: 0.58 ± 0.167
1.989SerTyr: 1.989 ± 0.475
0.0SerXaa: 0.0 ± 0.0
Thr
3.729ThrAla: 3.729 ± 0.537
0.414ThrCys: 0.414 ± 0.214
2.652ThrAsp: 2.652 ± 0.423
5.055ThrGlu: 5.055 ± 0.641
3.232ThrPhe: 3.232 ± 0.459
4.226ThrGly: 4.226 ± 0.599
1.243ThrHis: 1.243 ± 0.302
4.144ThrIle: 4.144 ± 0.573
4.972ThrLys: 4.972 ± 0.657
5.138ThrLeu: 5.138 ± 0.745
1.492ThrMet: 1.492 ± 0.367
2.569ThrAsn: 2.569 ± 0.523
1.989ThrPro: 1.989 ± 0.45
2.32ThrGln: 2.32 ± 0.531
1.492ThrArg: 1.492 ± 0.342
2.818ThrSer: 2.818 ± 0.427
4.144ThrThr: 4.144 ± 0.715
3.315ThrVal: 3.315 ± 0.684
0.58ThrTrp: 0.58 ± 0.254
1.575ThrTyr: 1.575 ± 0.356
0.0ThrXaa: 0.0 ± 0.0
Val
3.895ValAla: 3.895 ± 0.68
0.414ValCys: 0.414 ± 0.225
3.895ValAsp: 3.895 ± 0.548
6.215ValGlu: 6.215 ± 1.003
2.238ValPhe: 2.238 ± 0.391
3.232ValGly: 3.232 ± 0.463
0.663ValHis: 0.663 ± 0.226
3.646ValIle: 3.646 ± 0.484
5.635ValLys: 5.635 ± 0.56
4.475ValLeu: 4.475 ± 0.638
1.575ValMet: 1.575 ± 0.388
3.729ValAsn: 3.729 ± 0.487
2.155ValPro: 2.155 ± 0.39
3.563ValGln: 3.563 ± 0.45
3.646ValArg: 3.646 ± 0.577
3.563ValSer: 3.563 ± 0.417
3.481ValThr: 3.481 ± 0.498
3.729ValVal: 3.729 ± 0.548
0.166ValTrp: 0.166 ± 0.104
2.072ValTyr: 2.072 ± 0.4
0.0ValXaa: 0.0 ± 0.0
Trp
0.746TrpAla: 0.746 ± 0.231
0.166TrpCys: 0.166 ± 0.116
0.994TrpAsp: 0.994 ± 0.346
0.746TrpGlu: 0.746 ± 0.28
1.077TrpPhe: 1.077 ± 0.334
0.331TrpGly: 0.331 ± 0.145
0.166TrpHis: 0.166 ± 0.133
1.326TrpIle: 1.326 ± 0.401
0.994TrpLys: 0.994 ± 0.298
1.077TrpLeu: 1.077 ± 0.319
0.249TrpMet: 0.249 ± 0.161
0.746TrpAsn: 0.746 ± 0.177
0.083TrpPro: 0.083 ± 0.091
0.663TrpGln: 0.663 ± 0.232
1.077TrpArg: 1.077 ± 0.32
0.746TrpSer: 0.746 ± 0.228
0.414TrpThr: 0.414 ± 0.209
0.829TrpVal: 0.829 ± 0.312
0.083TrpTrp: 0.083 ± 0.102
0.829TrpTyr: 0.829 ± 0.31
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.072TyrAla: 2.072 ± 0.488
0.497TyrCys: 0.497 ± 0.202
2.155TyrAsp: 2.155 ± 0.603
3.563TyrGlu: 3.563 ± 0.662
1.409TyrPhe: 1.409 ± 0.339
2.238TyrGly: 2.238 ± 0.36
0.829TyrHis: 0.829 ± 0.347
2.735TyrIle: 2.735 ± 0.507
2.155TyrLys: 2.155 ± 0.337
2.072TyrLeu: 2.072 ± 0.416
1.077TyrMet: 1.077 ± 0.313
2.072TyrAsn: 2.072 ± 0.451
0.912TyrPro: 0.912 ± 0.327
2.072TyrGln: 2.072 ± 0.623
2.072TyrArg: 2.072 ± 0.358
1.492TyrSer: 1.492 ± 0.349
1.74TyrThr: 1.74 ± 0.35
2.32TyrVal: 2.32 ± 0.409
0.414TyrTrp: 0.414 ± 0.175
1.409TyrTyr: 1.409 ± 0.455
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 64 proteins (12068 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski