Glaciecola sp. (strain KUL10)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Glaciecola; unclassified Glaciecola

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3631 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z6UL02|A0A2Z6UL02_GLASK Phage-related protein OS=Glaciecola sp. (strain KUL10) OX=2161813 GN=KUL10_02120 PE=4 SV=1
MM1 pKa = 7.14QPNIAIFYY9 pKa = 10.46GSTTCYY15 pKa = 10.65TEE17 pKa = 3.71MAGEE21 pKa = 5.15KK22 pKa = 9.65IQQTLQKK29 pKa = 10.31QLGDD33 pKa = 3.39INIPMFDD40 pKa = 3.44IKK42 pKa = 10.77EE43 pKa = 4.03HH44 pKa = 6.39ALEE47 pKa = 4.74KK48 pKa = 10.5SQQFDD53 pKa = 3.7VIIFGISTWDD63 pKa = 3.43FGEE66 pKa = 4.3LQEE69 pKa = 5.04DD70 pKa = 4.32WEE72 pKa = 4.86STWEE76 pKa = 4.53DD77 pKa = 3.11IKK79 pKa = 11.44SLQLAGKK86 pKa = 7.52TVALYY91 pKa = 11.07GLGDD95 pKa = 3.62QLGYY99 pKa = 11.22ADD101 pKa = 4.61WFQDD105 pKa = 3.71ALGMLHH111 pKa = 7.41DD112 pKa = 4.64EE113 pKa = 5.49LVILDD118 pKa = 4.5CEE120 pKa = 4.79LVGYY124 pKa = 8.61WPNTGYY130 pKa = 11.12EE131 pKa = 4.28FTASKK136 pKa = 10.99ALTEE140 pKa = 4.16DD141 pKa = 3.03QQFFVGLSLDD151 pKa = 4.68DD152 pKa = 4.54EE153 pKa = 4.61NQFDD157 pKa = 3.99QSDD160 pKa = 3.47EE161 pKa = 5.47RR162 pKa = 11.84IQQWCQQLAEE172 pKa = 4.33EE173 pKa = 5.0LGNAQLSPYY182 pKa = 10.35

Molecular weight: 20.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z6UIG0|A0A2Z6UIG0_GLASK Glutamate 5-kinase OS=Glaciecola sp. (strain KUL10) OX=2161813 GN=proB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNIKK11 pKa = 10.11RR12 pKa = 11.84KK13 pKa = 9.04RR14 pKa = 11.84SHH16 pKa = 6.18GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 9.16VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.33GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3631

0

3631

1250739

44

4200

344.5

38.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.314 ± 0.041

0.954 ± 0.014

5.83 ± 0.033

6.328 ± 0.036

4.511 ± 0.03

6.355 ± 0.038

2.099 ± 0.02

6.688 ± 0.032

5.749 ± 0.037

10.069 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.414 ± 0.021

4.867 ± 0.035

3.621 ± 0.026

4.483 ± 0.033

4.195 ± 0.025

7.238 ± 0.036

5.369 ± 0.032

6.709 ± 0.031

1.12 ± 0.015

3.087 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski