Amino acid dipepetide frequency for Glaciecola sp. (strain KUL10)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.424AlaAla: 6.424 ± 0.101	1.008AlaCys: 1.008 ± 0.031	4.497AlaAsp: 4.497 ± 0.06	4.933AlaGlu: 4.933 ± 0.073	3.786AlaPhe: 3.786 ± 0.063	5.234AlaGly: 5.234 ± 0.088	1.555AlaHis: 1.555 ± 0.041	5.895AlaIle: 5.895 ± 0.078	5.037AlaLys: 5.037 ± 0.074	9.287AlaLeu: 9.287 ± 0.097	2.309AlaMet: 2.309 ± 0.043	4.109AlaAsn: 4.109 ± 0.056	2.667AlaPro: 2.667 ± 0.052	3.922AlaGln: 3.922 ± 0.057	3.425AlaArg: 3.425 ± 0.048	6.1AlaSer: 6.1 ± 0.073	4.184AlaThr: 4.184 ± 0.062	5.29AlaVal: 5.29 ± 0.069	0.893AlaTrp: 0.893 ± 0.025	2.582AlaTyr: 2.582 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	0.778CysAla: 0.778 ± 0.023	0.128CysCys: 0.128 ± 0.01	0.575CysAsp: 0.575 ± 0.022	0.624CysGlu: 0.624 ± 0.023	0.466CysPhe: 0.466 ± 0.018	0.676CysGly: 0.676 ± 0.028	0.288CysHis: 0.288 ± 0.015	0.673CysIle: 0.673 ± 0.024	0.486CysLys: 0.486 ± 0.02	1.002CysLeu: 1.002 ± 0.029	0.215CysMet: 0.215 ± 0.014	0.387CysAsn: 0.387 ± 0.018	0.374CysPro: 0.374 ± 0.017	0.364CysGln: 0.364 ± 0.019	0.373CysArg: 0.373 ± 0.019	0.684CysSer: 0.684 ± 0.025	0.426CysThr: 0.426 ± 0.018	0.652CysVal: 0.652 ± 0.025	0.087CysTrp: 0.087 ± 0.007	0.285CysTyr: 0.285 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	4.744AspAla: 4.744 ± 0.067	0.481AspCys: 0.481 ± 0.023	3.48AspAsp: 3.48 ± 0.066	4.315AspGlu: 4.315 ± 0.065	2.93AspPhe: 2.93 ± 0.051	3.828AspGly: 3.828 ± 0.079	0.952AspHis: 0.952 ± 0.03	4.543AspIle: 4.543 ± 0.064	3.559AspLys: 3.559 ± 0.067	5.494AspLeu: 5.494 ± 0.072	1.477AspMet: 1.477 ± 0.033	2.762AspAsn: 2.762 ± 0.049	2.007AspPro: 2.007 ± 0.048	1.879AspGln: 1.879 ± 0.032	2.067AspArg: 2.067 ± 0.043	3.667AspSer: 3.667 ± 0.057	3.106AspThr: 3.106 ± 0.051	3.945AspVal: 3.945 ± 0.063	0.875AspTrp: 0.875 ± 0.025	2.192AspTyr: 2.192 ± 0.043	0.0AspXaa: 0.0 ± 0.0
Glu	5.183GluAla: 5.183 ± 0.075	0.478GluCys: 0.478 ± 0.021	3.132GluAsp: 3.132 ± 0.059	3.627GluGlu: 3.627 ± 0.066	2.73GluPhe: 2.73 ± 0.051	3.284GluGly: 3.284 ± 0.062	1.642GluHis: 1.642 ± 0.039	4.136GluIle: 4.136 ± 0.067	4.006GluLys: 4.006 ± 0.066	6.935GluLeu: 6.935 ± 0.082	1.596GluMet: 1.596 ± 0.032	3.157GluAsn: 3.157 ± 0.051	1.823GluPro: 1.823 ± 0.044	3.934GluGln: 3.934 ± 0.061	3.041GluArg: 3.041 ± 0.053	4.221GluSer: 4.221 ± 0.066	3.332GluThr: 3.332 ± 0.056	4.253GluVal: 4.253 ± 0.063	0.712GluTrp: 0.712 ± 0.02	2.053GluTyr: 2.053 ± 0.043	0.0GluXaa: 0.0 ± 0.0
Phe	3.874PheAla: 3.874 ± 0.059	0.499PheCys: 0.499 ± 0.02	3.334PheAsp: 3.334 ± 0.056	3.285PheGlu: 3.285 ± 0.049	1.952PhePhe: 1.952 ± 0.045	3.239PheGly: 3.239 ± 0.064	0.768PheHis: 0.768 ± 0.026	3.106PheIle: 3.106 ± 0.063	2.499PheLys: 2.499 ± 0.044	3.618PheLeu: 3.618 ± 0.058	1.019PheMet: 1.019 ± 0.028	2.406PheAsn: 2.406 ± 0.043	1.426PhePro: 1.426 ± 0.032	1.253PheGln: 1.253 ± 0.036	1.481PheArg: 1.481 ± 0.035	3.731PheSer: 3.731 ± 0.057	2.463PheThr: 2.463 ± 0.049	3.158PheVal: 3.158 ± 0.059	0.491PheTrp: 0.491 ± 0.018	1.504PheTyr: 1.504 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	5.031GlyAla: 5.031 ± 0.066	0.703GlyCys: 0.703 ± 0.026	3.684GlyAsp: 3.684 ± 0.075	4.017GlyGlu: 4.017 ± 0.057	3.528GlyPhe: 3.528 ± 0.055	4.345GlyGly: 4.345 ± 0.083	1.402GlyHis: 1.402 ± 0.034	4.501GlyIle: 4.501 ± 0.068	3.755GlyLys: 3.755 ± 0.059	6.539GlyLeu: 6.539 ± 0.084	1.707GlyMet: 1.707 ± 0.042	2.691GlyAsn: 2.691 ± 0.054	1.504GlyPro: 1.504 ± 0.036	2.492GlyGln: 2.492 ± 0.051	2.652GlyArg: 2.652 ± 0.043	3.867GlySer: 3.867 ± 0.075	3.248GlyThr: 3.248 ± 0.055	4.797GlyVal: 4.797 ± 0.069	0.799GlyTrp: 0.799 ± 0.025	2.29GlyTyr: 2.29 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	1.598HisAla: 1.598 ± 0.038	0.256HisCys: 0.256 ± 0.016	1.099HisAsp: 1.099 ± 0.037	1.186HisGlu: 1.186 ± 0.034	1.074HisPhe: 1.074 ± 0.03	1.296HisGly: 1.296 ± 0.036	0.588HisHis: 0.588 ± 0.025	1.416HisIle: 1.416 ± 0.035	1.159HisLys: 1.159 ± 0.031	2.135HisLeu: 2.135 ± 0.051	0.475HisMet: 0.475 ± 0.018	0.896HisAsn: 0.896 ± 0.029	1.045HisPro: 1.045 ± 0.032	1.039HisGln: 1.039 ± 0.034	0.911HisArg: 0.911 ± 0.027	1.346HisSer: 1.346 ± 0.031	1.053HisThr: 1.053 ± 0.032	1.271HisVal: 1.271 ± 0.035	0.301HisTrp: 0.301 ± 0.017	0.844HisTyr: 0.844 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	6.258IleAla: 6.258 ± 0.084	0.731IleCys: 0.731 ± 0.025	4.909IleAsp: 4.909 ± 0.069	5.419IleGlu: 5.419 ± 0.065	2.366IlePhe: 2.366 ± 0.052	4.495IleGly: 4.495 ± 0.077	1.175IleHis: 1.175 ± 0.031	3.898IleIle: 3.898 ± 0.059	4.206IleLys: 4.206 ± 0.058	5.229IleLeu: 5.229 ± 0.078	1.284IleMet: 1.284 ± 0.031	3.736IleAsn: 3.736 ± 0.06	2.578IlePro: 2.578 ± 0.046	2.481IleGln: 2.481 ± 0.05	2.539IleArg: 2.539 ± 0.049	5.193IleSer: 5.193 ± 0.069	3.684IleThr: 3.684 ± 0.063	4.321IleVal: 4.321 ± 0.07	0.619IleTrp: 0.619 ± 0.025	1.761IleTyr: 1.761 ± 0.044	0.0IleXaa: 0.0 ± 0.0
Lys	5.192LysAla: 5.192 ± 0.082	0.381LysCys: 0.381 ± 0.016	3.064LysAsp: 3.064 ± 0.053	3.439LysGlu: 3.439 ± 0.057	1.86LysPhe: 1.86 ± 0.041	3.473LysGly: 3.473 ± 0.054	1.603LysHis: 1.603 ± 0.039	3.361LysIle: 3.361 ± 0.056	3.268LysLys: 3.268 ± 0.068	5.829LysLeu: 5.829 ± 0.077	1.385LysMet: 1.385 ± 0.034	2.531LysAsn: 2.531 ± 0.046	2.341LysPro: 2.341 ± 0.044	3.518LysGln: 3.518 ± 0.06	2.956LysArg: 2.956 ± 0.045	3.772LysSer: 3.772 ± 0.065	3.356LysThr: 3.356 ± 0.055	3.902LysVal: 3.902 ± 0.065	0.668LysTrp: 0.668 ± 0.024	1.593LysTyr: 1.593 ± 0.039	0.0LysXaa: 0.0 ± 0.0
Leu	8.996LeuAla: 8.996 ± 0.103	1.026LeuCys: 1.026 ± 0.024	5.797LeuAsp: 5.797 ± 0.068	5.968LeuGlu: 5.968 ± 0.073	4.451LeuPhe: 4.451 ± 0.075	6.428LeuGly: 6.428 ± 0.069	1.882LeuHis: 1.882 ± 0.039	6.417LeuIle: 6.417 ± 0.093	5.697LeuLys: 5.697 ± 0.083	9.692LeuLeu: 9.692 ± 0.118	2.375LeuMet: 2.375 ± 0.042	5.365LeuAsn: 5.365 ± 0.066	4.313LeuPro: 4.313 ± 0.063	3.64LeuGln: 3.64 ± 0.053	4.15LeuArg: 4.15 ± 0.062	8.798LeuSer: 8.798 ± 0.097	5.434LeuThr: 5.434 ± 0.063	6.755LeuVal: 6.755 ± 0.077	0.87LeuTrp: 0.87 ± 0.028	2.63LeuTyr: 2.63 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	2.008MetAla: 2.008 ± 0.044	0.208MetCys: 0.208 ± 0.013	1.123MetAsp: 1.123 ± 0.031	1.02MetGlu: 1.02 ± 0.028	0.983MetPhe: 0.983 ± 0.029	1.582MetGly: 1.582 ± 0.035	0.548MetHis: 0.548 ± 0.02	1.467MetIle: 1.467 ± 0.041	1.38MetLys: 1.38 ± 0.035	2.716MetLeu: 2.716 ± 0.05	0.652MetMet: 0.652 ± 0.022	1.165MetAsn: 1.165 ± 0.031	1.217MetPro: 1.217 ± 0.031	1.301MetGln: 1.301 ± 0.033	1.1MetArg: 1.1 ± 0.024	1.929MetSer: 1.929 ± 0.038	1.465MetThr: 1.465 ± 0.037	1.512MetVal: 1.512 ± 0.036	0.21MetTrp: 0.21 ± 0.014	0.553MetTyr: 0.553 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	4.143AsnAla: 4.143 ± 0.062	0.441AsnCys: 0.441 ± 0.021	2.874AsnAsp: 2.874 ± 0.049	3.267AsnGlu: 3.267 ± 0.045	1.884AsnPhe: 1.884 ± 0.043	3.256AsnGly: 3.256 ± 0.06	0.965AsnHis: 0.965 ± 0.025	3.343AsnIle: 3.343 ± 0.053	2.99AsnLys: 2.99 ± 0.055	4.366AsnLeu: 4.366 ± 0.06	1.126AsnMet: 1.126 ± 0.031	2.465AsnAsn: 2.465 ± 0.05	2.051AsnPro: 2.051 ± 0.043	2.495AsnGln: 2.495 ± 0.045	2.078AsnArg: 2.078 ± 0.04	3.054AsnSer: 3.054 ± 0.051	2.863AsnThr: 2.863 ± 0.051	2.942AsnVal: 2.942 ± 0.049	0.596AsnTrp: 0.596 ± 0.022	1.47AsnTyr: 1.47 ± 0.035	0.0AsnXaa: 0.0 ± 0.0
Pro	2.618ProAla: 2.618 ± 0.047	0.273ProCys: 0.273 ± 0.014	2.36ProAsp: 2.36 ± 0.044	2.753ProGlu: 2.753 ± 0.053	1.778ProPhe: 1.778 ± 0.038	1.92ProGly: 1.92 ± 0.045	0.699ProHis: 0.699 ± 0.024	2.658ProIle: 2.658 ± 0.051	2.05ProLys: 2.05 ± 0.043	3.52ProLeu: 3.52 ± 0.055	0.863ProMet: 0.863 ± 0.026	2.052ProAsn: 2.052 ± 0.041	1.074ProPro: 1.074 ± 0.032	1.434ProGln: 1.434 ± 0.031	1.22ProArg: 1.22 ± 0.035	2.874ProSer: 2.874 ± 0.049	2.008ProThr: 2.008 ± 0.046	2.545ProVal: 2.545 ± 0.043	0.397ProTrp: 0.397 ± 0.018	1.117ProTyr: 1.117 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	4.405GlnAla: 4.405 ± 0.068	0.344GlnCys: 0.344 ± 0.017	2.192GlnAsp: 2.192 ± 0.048	2.435GlnGlu: 2.435 ± 0.046	1.789GlnPhe: 1.789 ± 0.042	2.622GlnGly: 2.622 ± 0.05	1.083GlnHis: 1.083 ± 0.03	2.922GlnIle: 2.922 ± 0.049	2.383GlnLys: 2.383 ± 0.049	4.546GlnLeu: 4.546 ± 0.069	1.071GlnMet: 1.071 ± 0.03	2.047GlnAsn: 2.047 ± 0.04	1.317GlnPro: 1.317 ± 0.033	2.654GlnGln: 2.654 ± 0.05	2.14GlnArg: 2.14 ± 0.041	3.268GlnSer: 3.268 ± 0.057	2.711GlnThr: 2.711 ± 0.051	2.974GlnVal: 2.974 ± 0.045	0.54GlnTrp: 0.54 ± 0.02	1.382GlnTyr: 1.382 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	3.262ArgAla: 3.262 ± 0.047	0.349ArgCys: 0.349 ± 0.017	2.268ArgAsp: 2.268 ± 0.042	2.631ArgGlu: 2.631 ± 0.05	2.328ArgPhe: 2.328 ± 0.048	2.376ArgGly: 2.376 ± 0.05	0.909ArgHis: 0.909 ± 0.03	2.913ArgIle: 2.913 ± 0.052	2.283ArgLys: 2.283 ± 0.052	4.692ArgLeu: 4.692 ± 0.067	1.011ArgMet: 1.011 ± 0.03	1.909ArgAsn: 1.909 ± 0.038	1.482ArgPro: 1.482 ± 0.036	1.914ArgGln: 1.914 ± 0.039	1.96ArgArg: 1.96 ± 0.044	2.51ArgSer: 2.51 ± 0.048	1.993ArgThr: 1.993 ± 0.044	3.009ArgVal: 3.009 ± 0.047	0.518ArgTrp: 0.518 ± 0.021	1.629ArgTyr: 1.629 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	5.999SerAla: 5.999 ± 0.079	0.575SerCys: 0.575 ± 0.022	4.277SerAsp: 4.277 ± 0.067	4.497SerGlu: 4.497 ± 0.071	3.461SerPhe: 3.461 ± 0.062	4.834SerGly: 4.834 ± 0.066	1.403SerHis: 1.403 ± 0.035	5.078SerIle: 5.078 ± 0.07	4.066SerLys: 4.066 ± 0.06	7.799SerLeu: 7.799 ± 0.089	1.81SerMet: 1.81 ± 0.038	3.373SerAsn: 3.373 ± 0.058	2.47SerPro: 2.47 ± 0.042	3.139SerGln: 3.139 ± 0.053	2.764SerArg: 2.764 ± 0.052	5.153SerSer: 5.153 ± 0.078	3.813SerThr: 3.813 ± 0.057	5.024SerVal: 5.024 ± 0.07	0.732SerTrp: 0.732 ± 0.023	2.11SerTyr: 2.11 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	4.081ThrAla: 4.081 ± 0.062	0.478ThrCys: 0.478 ± 0.018	3.029ThrAsp: 3.029 ± 0.059	3.102ThrGlu: 3.102 ± 0.052	2.302ThrPhe: 2.302 ± 0.049	3.797ThrGly: 3.797 ± 0.061	1.283ThrHis: 1.283 ± 0.033	3.59ThrIle: 3.59 ± 0.059	2.809ThrLys: 2.809 ± 0.051	5.954ThrLeu: 5.954 ± 0.073	1.087ThrMet: 1.087 ± 0.028	2.499ThrAsn: 2.499 ± 0.045	2.524ThrPro: 2.524 ± 0.045	2.674ThrGln: 2.674 ± 0.05	2.21ThrArg: 2.21 ± 0.036	3.82ThrSer: 3.82 ± 0.058	2.752ThrThr: 2.752 ± 0.053	3.483ThrVal: 3.483 ± 0.062	0.635ThrTrp: 0.635 ± 0.026	1.584ThrTyr: 1.584 ± 0.034	0.0ThrXaa: 0.0 ± 0.0
Val	5.465ValAla: 5.465 ± 0.089	0.734ValCys: 0.734 ± 0.026	4.206ValAsp: 4.206 ± 0.058	4.329ValGlu: 4.329 ± 0.079	3.273ValPhe: 3.273 ± 0.053	4.225ValGly: 4.225 ± 0.065	1.228ValHis: 1.228 ± 0.034	4.636ValIle: 4.636 ± 0.061	3.747ValLys: 3.747 ± 0.047	6.573ValLeu: 6.573 ± 0.092	1.685ValMet: 1.685 ± 0.037	3.48ValAsn: 3.48 ± 0.066	2.379ValPro: 2.379 ± 0.044	2.115ValGln: 2.115 ± 0.04	2.623ValArg: 2.623 ± 0.054	5.423ValSer: 5.423 ± 0.062	3.761ValThr: 3.761 ± 0.064	4.652ValVal: 4.652 ± 0.067	0.67ValTrp: 0.67 ± 0.022	1.888ValTyr: 1.888 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	0.704TrpAla: 0.704 ± 0.024	0.119TrpCys: 0.119 ± 0.011	0.6TrpAsp: 0.6 ± 0.026	0.546TrpGlu: 0.546 ± 0.02	0.594TrpPhe: 0.594 ± 0.02	0.704TrpGly: 0.704 ± 0.025	0.3TrpHis: 0.3 ± 0.017	0.62TrpIle: 0.62 ± 0.023	0.491TrpLys: 0.491 ± 0.02	1.45TrpLeu: 1.45 ± 0.037	0.338TrpMet: 0.338 ± 0.017	0.435TrpAsn: 0.435 ± 0.02	0.387TrpPro: 0.387 ± 0.02	0.78TrpGln: 0.78 ± 0.027	0.636TrpArg: 0.636 ± 0.025	0.692TrpSer: 0.692 ± 0.026	0.505TrpThr: 0.505 ± 0.019	0.758TrpVal: 0.758 ± 0.021	0.166TrpTrp: 0.166 ± 0.013	0.379TrpTyr: 0.379 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.373TyrAla: 2.373 ± 0.042	0.334TyrCys: 0.334 ± 0.015	1.802TyrAsp: 1.802 ± 0.043	1.884TyrGlu: 1.884 ± 0.036	1.571TyrPhe: 1.571 ± 0.037	1.939TyrGly: 1.939 ± 0.04	0.715TyrHis: 0.715 ± 0.025	1.706TyrIle: 1.706 ± 0.04	1.621TyrLys: 1.621 ± 0.037	3.311TyrLeu: 3.311 ± 0.059	0.654TyrMet: 0.654 ± 0.022	1.204TyrAsn: 1.204 ± 0.033	1.234TyrPro: 1.234 ± 0.03	1.798TyrGln: 1.798 ± 0.035	1.619TyrArg: 1.619 ± 0.04	2.274TyrSer: 2.274 ± 0.044	1.535TyrThr: 1.535 ± 0.034	1.847TyrVal: 1.847 ± 0.038	0.425TyrTrp: 0.425 ± 0.022	1.025TyrTyr: 1.025 ± 0.032	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3631 proteins (1250739 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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