Capybara microvirus Cap1_SP_104
Average proteome isoelectric point is 5.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVN7|A0A4V1FVN7_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_104 OX=2584803 PE=4 SV=1
MM1 pKa = 7.46 SVLDD5 pKa = 4.73 PIRR8 pKa = 11.84 SRR10 pKa = 11.84 ISEE13 pKa = 3.94 EE14 pKa = 3.53 MLYY17 pKa = 10.99 DD18 pKa = 4.11 GMHH21 pKa = 6.15 EE22 pKa = 4.01 QLDD25 pKa = 4.0 LRR27 pKa = 11.84 ILLSADD33 pKa = 3.19 GKK35 pKa = 10.12 PILTHH40 pKa = 5.58 YY41 pKa = 10.49 VPSLEE46 pKa = 4.17 LSSMYY51 pKa = 10.68 DD52 pKa = 3.56 LEE54 pKa = 5.27 SMLKK58 pKa = 10.52 VGITPSGSLHH68 pKa = 6.86 CDD70 pKa = 2.9 GNSRR74 pKa = 11.84 LDD76 pKa = 3.55 SFEE79 pKa = 5.59 SNITDD84 pKa = 4.16 FQTLADD90 pKa = 4.24 SFTDD94 pKa = 3.4 KK95 pKa = 11.3 PEE97 pKa = 3.65
Molecular weight: 10.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.369
IPC2_protein 4.342
IPC_protein 4.253
Toseland 4.075
ProMoST 4.329
Dawson 4.228
Bjellqvist 4.444
Wikipedia 4.151
Rodwell 4.088
Grimsley 3.986
Solomon 4.228
Lehninger 4.177
Nozaki 4.342
DTASelect 4.558
Thurlkill 4.113
EMBOSS 4.164
Sillero 4.38
Patrickios 3.656
IPC_peptide 4.228
IPC2_peptide 4.355
IPC2.peptide.svr19 4.296
Protein with the highest isoelectric point:
>tr|A0A4P8W4W9|A0A4P8W4W9_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_104 OX=2584803 PE=4 SV=1
MM1 pKa = 7.17 LTSDD5 pKa = 4.99 LIRR8 pKa = 11.84 NKK10 pKa = 10.1 SCYY13 pKa = 10.11 YY14 pKa = 9.26 YY15 pKa = 8.12 PTRR18 pKa = 11.84 NRR20 pKa = 11.84 SHH22 pKa = 6.46 FRR24 pKa = 11.84 VSRR27 pKa = 11.84 SDD29 pKa = 3.68 LYY31 pKa = 11.07 SSRR34 pKa = 11.84 VSKK37 pKa = 10.52 ILSYY41 pKa = 9.63 SVRR44 pKa = 11.84 LYY46 pKa = 11.42 YY47 pKa = 10.44 EE48 pKa = 3.91 FLEE51 pKa = 4.49 CTEE54 pKa = 3.93 CGGRR58 pKa = 11.84 VFFYY62 pKa = 10.57 TLTYY66 pKa = 10.62 NDD68 pKa = 4.14 LSIPTLYY75 pKa = 10.81 GCPCFDD81 pKa = 4.1 YY82 pKa = 11.4 NDD84 pKa = 3.25 IRR86 pKa = 11.84 DD87 pKa = 4.1 FVHH90 pKa = 7.13 GAFQKK95 pKa = 10.69 KK96 pKa = 8.71 LCRR99 pKa = 11.84 DD100 pKa = 4.7 FGCTMKK106 pKa = 10.95 YY107 pKa = 10.67 AVFCEE112 pKa = 3.9 RR113 pKa = 11.84 GEE115 pKa = 4.42 GKK117 pKa = 10.41 GKK119 pKa = 10.45 RR120 pKa = 11.84 GFGNNPHH127 pKa = 5.7 YY128 pKa = 10.68 HH129 pKa = 5.9 VIFYY133 pKa = 9.23 LYY135 pKa = 9.41 PKK137 pKa = 9.23 EE138 pKa = 3.99 GKK140 pKa = 10.29 RR141 pKa = 11.84 FDD143 pKa = 3.99 FVADD147 pKa = 3.71 EE148 pKa = 4.26 VTLLVRR154 pKa = 11.84 KK155 pKa = 9.14 YY156 pKa = 8.67 WQGDD160 pKa = 3.54 CRR162 pKa = 11.84 PFDD165 pKa = 3.74 VDD167 pKa = 3.86 GVNPQSFKK175 pKa = 11.12 YY176 pKa = 10.62 GIVQVGKK183 pKa = 10.46 YY184 pKa = 9.18 GAEE187 pKa = 3.86 ALDD190 pKa = 3.56 YY191 pKa = 10.97 RR192 pKa = 11.84 AFRR195 pKa = 11.84 YY196 pKa = 8.28 CAKK199 pKa = 10.6 YY200 pKa = 9.96 CVKK203 pKa = 10.55 DD204 pKa = 3.57 AADD207 pKa = 3.65 LSVNRR212 pKa = 11.84 QIWSSFLKK220 pKa = 10.75 DD221 pKa = 3.46 FEE223 pKa = 4.5 EE224 pKa = 4.76 SVKK227 pKa = 10.67 NDD229 pKa = 2.96 QVLIDD234 pKa = 3.83 KK235 pKa = 10.62 YY236 pKa = 10.47 MYY238 pKa = 11.0 AHH240 pKa = 6.14 NQYY243 pKa = 10.46 SCSIRR248 pKa = 11.84 DD249 pKa = 3.56 AEE251 pKa = 4.88 LSRR254 pKa = 11.84 GLPKK258 pKa = 10.26 RR259 pKa = 11.84 EE260 pKa = 3.53 FTFFDD265 pKa = 4.02 CATNYY270 pKa = 9.74 HH271 pKa = 6.56 RR272 pKa = 11.84 FFEE275 pKa = 4.23 FHH277 pKa = 7.54 KK278 pKa = 10.65 DD279 pKa = 3.06 TLQPILDD286 pKa = 3.93 KK287 pKa = 11.02 EE288 pKa = 4.11 RR289 pKa = 11.84 SLFLNRR295 pKa = 11.84 FCARR299 pKa = 11.84 VRR301 pKa = 11.84 ISQGVGISALKK312 pKa = 10.34 KK313 pKa = 10.65 VIEE316 pKa = 4.85 HH317 pKa = 7.59 DD318 pKa = 4.26 DD319 pKa = 3.86 GSLSVPLPSKK329 pKa = 11.0 NSFEE333 pKa = 5.48 DD334 pKa = 3.36 IRR336 pKa = 11.84 ISDD339 pKa = 3.65 YY340 pKa = 11.34 LLRR343 pKa = 11.84 KK344 pKa = 9.65 LLYY347 pKa = 9.82 DD348 pKa = 3.64 VKK350 pKa = 10.8 KK351 pKa = 11.01 DD352 pKa = 3.82 PITGNNYY359 pKa = 7.73 YY360 pKa = 9.25 TLNEE364 pKa = 4.05 YY365 pKa = 9.79 GQSIKK370 pKa = 10.06 MKK372 pKa = 10.01 QLQPSIARR380 pKa = 11.84 LADD383 pKa = 3.32 KK384 pKa = 10.83 AFSLVSSNPDD394 pKa = 3.06 YY395 pKa = 11.35 NSIYY399 pKa = 10.28 EE400 pKa = 4.46 SYY402 pKa = 11.21 SSADD406 pKa = 2.94 HH407 pKa = 6.96 DD408 pKa = 4.44 FRR410 pKa = 11.84 WSSGAMLRR418 pKa = 11.84 SALSSCTLEE427 pKa = 4.36 LCSLYY432 pKa = 11.09 ALYY435 pKa = 10.73 KK436 pKa = 9.94 KK437 pKa = 10.61 VYY439 pKa = 8.21 EE440 pKa = 4.26 FRR442 pKa = 11.84 SFAIRR447 pKa = 11.84 DD448 pKa = 3.7 FQFDD452 pKa = 4.3 GVPSLDD458 pKa = 3.88 PLADD462 pKa = 3.76 YY463 pKa = 11.26 YY464 pKa = 11.21 STLFDD469 pKa = 3.36 FHH471 pKa = 8.01 QPILFGQFYY480 pKa = 10.25 SHH482 pKa = 7.78 DD483 pKa = 3.68 GSEE486 pKa = 4.37 CFDD489 pKa = 4.58 PYY491 pKa = 10.67 FIPYY495 pKa = 8.82 SAHH498 pKa = 7.2 PIFAPHH504 pKa = 6.7 FDD506 pKa = 4.09 SFCALDD512 pKa = 3.56 ILLDD516 pKa = 3.64 YY517 pKa = 10.27 ATFQDD522 pKa = 3.67 QEE524 pKa = 4.37 VKK526 pKa = 10.44 SRR528 pKa = 11.84 RR529 pKa = 11.84 DD530 pKa = 3.14 AEE532 pKa = 3.75 NRR534 pKa = 11.84 RR535 pKa = 11.84 IKK537 pKa = 10.69 NFHH540 pKa = 5.78 NNKK543 pKa = 9.38 KK544 pKa = 9.23 FKK546 pKa = 10.25 HH547 pKa = 5.69 YY548 pKa = 10.36 SLCPFF553 pKa = 4.6
Molecular weight: 64.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.207
IPC2_protein 7.41
IPC_protein 7.205
Toseland 6.854
ProMoST 7.79
Dawson 7.907
Bjellqvist 8.346
Wikipedia 7.819
Rodwell 7.922
Grimsley 6.912
Solomon 7.936
Lehninger 7.951
Nozaki 8.492
DTASelect 8.053
Thurlkill 8.097
EMBOSS 8.126
Sillero 8.434
Patrickios 3.528
IPC_peptide 7.936
IPC2_peptide 7.468
IPC2.peptide.svr19 7.433
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1666
97
610
416.5
47.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.942 ± 1.041
1.801 ± 0.673
6.723 ± 0.765
5.402 ± 0.92
6.002 ± 1.242
5.402 ± 0.702
1.981 ± 0.409
5.222 ± 0.409
5.582 ± 0.631
9.364 ± 0.471
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.281 ± 0.611
5.222 ± 0.64
4.262 ± 0.267
3.842 ± 1.295
5.402 ± 0.529
10.264 ± 0.463
4.262 ± 0.581
4.562 ± 0.342
0.96 ± 0.251
5.522 ± 0.891
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here