Dethiosulfovibrio peptidovorans DSM 11002
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2432 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D2Z462|D2Z462_9BACT Dihydroorotase and related cyclic amidohydrolase-like protein OS=Dethiosulfovibrio peptidovorans DSM 11002 OX=469381 GN=Dpep_2301 PE=4 SV=1
MM1 pKa = 7.59 SARR4 pKa = 11.84 EE5 pKa = 3.8 KK6 pKa = 10.14 IAYY9 pKa = 9.22 IKK11 pKa = 10.83 GLLDD15 pKa = 3.96 AGTPRR20 pKa = 11.84 DD21 pKa = 4.14 DD22 pKa = 5.68 FEE24 pKa = 4.18 MALYY28 pKa = 10.02 GAIVEE33 pKa = 4.44 ALDD36 pKa = 4.69 AVVDD40 pKa = 4.0 DD41 pKa = 5.09 MDD43 pKa = 4.28 RR44 pKa = 11.84 QDD46 pKa = 3.63 EE47 pKa = 4.54 SLVALTEE54 pKa = 3.95 EE55 pKa = 5.01 LMDD58 pKa = 5.24 LSDD61 pKa = 3.87 YY62 pKa = 11.3 CDD64 pKa = 3.79 GLGEE68 pKa = 4.53 DD69 pKa = 4.56 LDD71 pKa = 5.2 AIEE74 pKa = 4.89 EE75 pKa = 4.5 GWLGEE80 pKa = 4.04 RR81 pKa = 11.84 VLDD84 pKa = 4.55 QEE86 pKa = 4.84 DD87 pKa = 3.69 VLIAEE92 pKa = 4.53 GDD94 pKa = 3.71 EE95 pKa = 4.25 PEE97 pKa = 5.13 GVDD100 pKa = 3.73 LYY102 pKa = 11.35 RR103 pKa = 11.84 PILCPYY109 pKa = 8.66 CSTMFYY115 pKa = 10.56 YY116 pKa = 10.49 RR117 pKa = 11.84 PDD119 pKa = 3.52 LCDD122 pKa = 3.72 EE123 pKa = 4.74 NDD125 pKa = 3.55 TAQCPNCRR133 pKa = 11.84 RR134 pKa = 11.84 TFAPSEE140 pKa = 4.22 VEE142 pKa = 4.18 LEE144 pKa = 4.27 EE145 pKa = 4.28 EE146 pKa = 4.21
Molecular weight: 16.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.643
IPC_protein 3.63
Toseland 3.427
ProMoST 3.77
Dawson 3.617
Bjellqvist 3.808
Wikipedia 3.541
Rodwell 3.465
Grimsley 3.338
Solomon 3.605
Lehninger 3.554
Nozaki 3.732
DTASelect 3.935
Thurlkill 3.478
EMBOSS 3.554
Sillero 3.745
Patrickios 0.731
IPC_peptide 3.605
IPC2_peptide 3.732
IPC2.peptide.svr19 3.718
Protein with the highest isoelectric point:
>tr|D2Z2P3|D2Z2P3_9BACT Deoxyuridine 5'-triphosphate nucleotidohydrolase OS=Dethiosulfovibrio peptidovorans DSM 11002 OX=469381 GN=dut PE=3 SV=1
MM1 pKa = 7.26 KK2 pKa = 9.53 QTFQPHH8 pKa = 4.48 NRR10 pKa = 11.84 PRR12 pKa = 11.84 KK13 pKa = 8.83 RR14 pKa = 11.84 KK15 pKa = 8.04 MGFLARR21 pKa = 11.84 SSSPSGRR28 pKa = 11.84 RR29 pKa = 11.84 ILANRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.82 GRR39 pKa = 11.84 KK40 pKa = 8.69 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2432
0
2432
782988
30
1943
322.0
35.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.151 ± 0.05
1.206 ± 0.018
6.162 ± 0.041
6.971 ± 0.048
3.785 ± 0.031
8.71 ± 0.042
1.584 ± 0.017
5.824 ± 0.04
4.925 ± 0.039
10.169 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.097 ± 0.021
2.655 ± 0.029
4.399 ± 0.033
2.22 ± 0.023
6.442 ± 0.053
7.138 ± 0.048
4.518 ± 0.035
8.168 ± 0.041
1.286 ± 0.021
2.59 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here