Fervidicola ferrireducens
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2421 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140L6A5|A0A140L6A5_9FIRM Uncharacterized protein OS=Fervidicola ferrireducens OX=520764 GN=AN618_16680 PE=4 SV=1
MM1 pKa = 7.38 KK2 pKa = 10.23 KK3 pKa = 9.14 WVCTVCGYY11 pKa = 11.14 VYY13 pKa = 10.74 DD14 pKa = 4.65 PDD16 pKa = 5.75 KK17 pKa = 11.41 GDD19 pKa = 4.02 PDD21 pKa = 3.71 NGIEE25 pKa = 4.46 PGTSFDD31 pKa = 5.6 DD32 pKa = 6.0 LPDD35 pKa = 4.02 DD36 pKa = 3.81 WTCPVCGAGKK46 pKa = 10.55 DD47 pKa = 3.64 MFEE50 pKa = 4.42 EE51 pKa = 4.5 EE52 pKa = 4.21
Molecular weight: 5.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.745
IPC_protein 3.681
Toseland 3.465
ProMoST 3.834
Dawson 3.694
Bjellqvist 3.935
Wikipedia 3.668
Rodwell 3.516
Grimsley 3.389
Solomon 3.668
Lehninger 3.617
Nozaki 3.834
DTASelect 4.062
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.808
Patrickios 0.693
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.785
Protein with the highest isoelectric point:
>tr|A0A140L4E1|A0A140L4E1_9FIRM Orotate phosphoribosyltransferase OS=Fervidicola ferrireducens OX=520764 GN=pyrE PE=3 SV=1
MM1 pKa = 7.07 GHH3 pKa = 6.96 KK4 pKa = 9.87 NLKK7 pKa = 9.57 QLDD10 pKa = 4.39 AIAFKK15 pKa = 10.69 PGDD18 pKa = 3.4 EE19 pKa = 4.27 SGFRR23 pKa = 11.84 LCYY26 pKa = 9.39 NGSMKK31 pKa = 9.09 HH32 pKa = 4.66 TNVVRR37 pKa = 11.84 VLPDD41 pKa = 3.1 KK42 pKa = 10.6 RR43 pKa = 11.84 QKK45 pKa = 10.27 QVLEE49 pKa = 4.47 IIGDD53 pKa = 3.87 RR54 pKa = 11.84 CAALWNAVQYY64 pKa = 10.49 RR65 pKa = 11.84 CRR67 pKa = 11.84 QAFFKK72 pKa = 10.78 GDD74 pKa = 3.82 SVPSYY79 pKa = 11.1 AALCSEE85 pKa = 4.81 FKK87 pKa = 10.5 EE88 pKa = 4.38 HH89 pKa = 6.94 PAYY92 pKa = 9.76 KK93 pKa = 10.27 ALPAHH98 pKa = 7.2 IGQEE102 pKa = 4.36 VIKK105 pKa = 10.0 KK106 pKa = 9.63 ARR108 pKa = 11.84 KK109 pKa = 8.86 AWDD112 pKa = 3.36 SYY114 pKa = 9.23 FACLRR119 pKa = 11.84 LYY121 pKa = 10.7 RR122 pKa = 11.84 KK123 pKa = 10.33 GEE125 pKa = 4.15 LKK127 pKa = 10.46 EE128 pKa = 4.15 PPHH131 pKa = 7.12 IPRR134 pKa = 11.84 YY135 pKa = 8.09 WKK137 pKa = 10.1 DD138 pKa = 2.87 RR139 pKa = 11.84 RR140 pKa = 11.84 TGRR143 pKa = 11.84 RR144 pKa = 11.84 IFRR147 pKa = 11.84 VIPVKK152 pKa = 10.88 APTSYY157 pKa = 11.47 SLDD160 pKa = 3.52 ARR162 pKa = 11.84 CLSLTLPQDD171 pKa = 3.42 LRR173 pKa = 11.84 KK174 pKa = 10.12 GQGDD178 pKa = 3.69 RR179 pKa = 11.84 LVLRR183 pKa = 11.84 TKK185 pKa = 10.68 GVLRR189 pKa = 11.84 FHH191 pKa = 6.96 GRR193 pKa = 11.84 PKK195 pKa = 10.14 TLEE198 pKa = 3.93 LKK200 pKa = 10.2 YY201 pKa = 11.07 DD202 pKa = 3.65 PVKK205 pKa = 10.08 TRR207 pKa = 11.84 WYY209 pKa = 7.9 AHH211 pKa = 5.21 QVVEE215 pKa = 4.35 VPEE218 pKa = 4.41 PVRR221 pKa = 11.84 KK222 pKa = 9.53 ARR224 pKa = 11.84 PEE226 pKa = 3.42 KK227 pKa = 10.39 HH228 pKa = 6.06 AAIDD232 pKa = 3.44 LGARR236 pKa = 11.84 VLVALAVEE244 pKa = 4.52 GLNRR248 pKa = 11.84 QLLFSARR255 pKa = 11.84 EE256 pKa = 3.87 VIKK259 pKa = 10.97 DD260 pKa = 3.21 FLYY263 pKa = 8.04 WTNQIAGEE271 pKa = 3.94 QSRR274 pKa = 11.84 LNRR277 pKa = 11.84 AGRR280 pKa = 11.84 KK281 pKa = 5.74 TSRR284 pKa = 11.84 RR285 pKa = 11.84 LKK287 pKa = 10.42 RR288 pKa = 11.84 LYY290 pKa = 9.27 QLRR293 pKa = 11.84 ARR295 pKa = 11.84 RR296 pKa = 11.84 LRR298 pKa = 11.84 HH299 pKa = 5.83 AFVALAAEE307 pKa = 4.62 IVRR310 pKa = 11.84 ILKK313 pKa = 10.1 RR314 pKa = 11.84 LRR316 pKa = 11.84 VTTLFLEE323 pKa = 4.57 DD324 pKa = 3.17 LTGIRR329 pKa = 11.84 EE330 pKa = 4.28 AMDD333 pKa = 3.97 FGPKK337 pKa = 9.82 NLLVHH342 pKa = 6.29 NFWAFRR348 pKa = 11.84 MLRR351 pKa = 11.84 NLIEE355 pKa = 4.26 AACTRR360 pKa = 11.84 TGIRR364 pKa = 11.84 VVPVEE369 pKa = 4.28 PRR371 pKa = 11.84 GTSCRR376 pKa = 11.84 CAVCGSSVRR385 pKa = 11.84 RR386 pKa = 11.84 PVRR389 pKa = 11.84 HH390 pKa = 5.98 KK391 pKa = 10.61 AVCEE395 pKa = 3.58 KK396 pKa = 10.6 CGRR399 pKa = 11.84 VWHH402 pKa = 7.0 ADD404 pKa = 2.81 ANAALNIILQGSSKK418 pKa = 10.18 EE419 pKa = 4.16 HH420 pKa = 5.99 GAEE423 pKa = 3.89 ATPLKK428 pKa = 10.28 PLALRR433 pKa = 11.84 WDD435 pKa = 3.41 RR436 pKa = 11.84 HH437 pKa = 4.06 RR438 pKa = 11.84 WVSRR442 pKa = 11.84 FEE444 pKa = 4.28 SAAGTPSGATSGSRR458 pKa = 11.84 MAAA461 pKa = 3.18
Molecular weight: 52.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.663
IPC_protein 10.35
Toseland 10.73
ProMoST 10.379
Dawson 10.818
Bjellqvist 10.511
Wikipedia 11.008
Rodwell 11.082
Grimsley 10.847
Solomon 10.921
Lehninger 10.891
Nozaki 10.73
DTASelect 10.496
Thurlkill 10.716
EMBOSS 11.125
Sillero 10.745
Patrickios 10.774
IPC_peptide 10.935
IPC2_peptide 9.575
IPC2.peptide.svr19 8.631
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2421
0
2421
709530
30
2290
293.1
32.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.773 ± 0.059
1.063 ± 0.023
4.862 ± 0.031
8.074 ± 0.066
4.242 ± 0.04
7.569 ± 0.044
1.472 ± 0.02
7.876 ± 0.045
7.745 ± 0.046
9.773 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.629 ± 0.023
3.703 ± 0.028
4.007 ± 0.029
2.376 ± 0.024
5.179 ± 0.044
5.298 ± 0.033
4.471 ± 0.036
7.789 ± 0.047
0.786 ± 0.017
3.316 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here