Rhizobacter gummiphilus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Rhizobacter

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5678 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6LA27|A0A1W6LA27_9BURK ABC transporter substrate-binding protein OS=Rhizobacter gummiphilus OX=946333 GN=A4W93_15325 PE=4 SV=1
MM1 pKa = 6.89TTTALPDD8 pKa = 4.5PDD10 pKa = 4.5PMPVAPEE17 pKa = 4.22PPDD20 pKa = 5.0LDD22 pKa = 3.74EE23 pKa = 5.14CCGNGCDD30 pKa = 3.59PCIFDD35 pKa = 4.89LHH37 pKa = 6.93DD38 pKa = 4.08LAMDD42 pKa = 5.13AYY44 pKa = 10.33RR45 pKa = 11.84QDD47 pKa = 2.94LRR49 pKa = 11.84AWRR52 pKa = 11.84EE53 pKa = 3.72RR54 pKa = 11.84HH55 pKa = 5.95PEE57 pKa = 3.64ASAGGG62 pKa = 3.6

Molecular weight: 6.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6L2W4|A0A1W6L2W4_9BURK DUF4124 domain-containing protein OS=Rhizobacter gummiphilus OX=946333 GN=A4W93_01075 PE=4 SV=1
MM1 pKa = 7.96ALLQPQARR9 pKa = 11.84PLAHH13 pKa = 7.0PASTRR18 pKa = 11.84QPVMPTGVRR27 pKa = 11.84PMPPAPARR35 pKa = 11.84AFQVLQTHH43 pKa = 6.01RR44 pKa = 11.84TPTQLSLFAPPVAQRR59 pKa = 11.84NVTRR63 pKa = 4.5

Molecular weight: 6.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5678

0

5678

1907566

30

5804

336.0

36.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.16 ± 0.049

0.813 ± 0.012

5.577 ± 0.023

5.048 ± 0.033

3.511 ± 0.02

8.567 ± 0.033

2.277 ± 0.019

3.753 ± 0.022

2.888 ± 0.028

10.66 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.236 ± 0.016

2.426 ± 0.025

5.565 ± 0.03

3.461 ± 0.021

7.28 ± 0.041

5.281 ± 0.029

5.598 ± 0.053

8.342 ± 0.028

1.51 ± 0.016

2.047 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski