Amino acid dipepetide frequency for Ralstonia phage RSJ5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.279AlaAla: 17.279 ± 2.314
1.05AlaCys: 1.05 ± 0.28
6.702AlaAsp: 6.702 ± 0.735
5.975AlaGlu: 5.975 ± 0.877
2.826AlaPhe: 2.826 ± 0.564
10.335AlaGly: 10.335 ± 1.295
1.453AlaHis: 1.453 ± 0.431
5.652AlaIle: 5.652 ± 0.633
6.863AlaLys: 6.863 ± 0.646
9.285AlaLeu: 9.285 ± 0.932
3.956AlaMet: 3.956 ± 0.763
4.925AlaAsn: 4.925 ± 0.792
3.553AlaPro: 3.553 ± 0.442
4.845AlaGln: 4.845 ± 0.832
7.186AlaArg: 7.186 ± 1.082
4.845AlaSer: 4.845 ± 0.681
7.671AlaThr: 7.671 ± 1.285
8.155AlaVal: 8.155 ± 0.864
1.696AlaTrp: 1.696 ± 0.287
3.149AlaTyr: 3.149 ± 0.509
0.0AlaXaa: 0.0 ± 0.0
Cys
0.565CysAla: 0.565 ± 0.185
0.161CysCys: 0.161 ± 0.13
0.484CysAsp: 0.484 ± 0.178
0.242CysGlu: 0.242 ± 0.141
0.081CysPhe: 0.081 ± 0.078
0.888CysGly: 0.888 ± 0.352
0.323CysHis: 0.323 ± 0.168
0.646CysIle: 0.646 ± 0.206
0.161CysLys: 0.161 ± 0.107
0.565CysLeu: 0.565 ± 0.227
0.404CysMet: 0.404 ± 0.198
0.323CysAsn: 0.323 ± 0.149
0.484CysPro: 0.484 ± 0.195
0.323CysGln: 0.323 ± 0.15
0.161CysArg: 0.161 ± 0.116
0.242CysSer: 0.242 ± 0.152
0.969CysThr: 0.969 ± 0.273
0.727CysVal: 0.727 ± 0.247
0.242CysTrp: 0.242 ± 0.154
0.242CysTyr: 0.242 ± 0.129
0.0CysXaa: 0.0 ± 0.0
Asp
7.428AspAla: 7.428 ± 0.721
1.13AspCys: 1.13 ± 0.338
3.956AspAsp: 3.956 ± 0.572
3.795AspGlu: 3.795 ± 0.69
2.422AspPhe: 2.422 ± 0.311
5.571AspGly: 5.571 ± 0.773
1.13AspHis: 1.13 ± 0.339
2.907AspIle: 2.907 ± 0.46
2.665AspLys: 2.665 ± 0.519
4.199AspLeu: 4.199 ± 0.639
1.696AspMet: 1.696 ± 0.422
2.099AspAsn: 2.099 ± 0.431
3.714AspPro: 3.714 ± 0.525
2.584AspGln: 2.584 ± 0.444
4.118AspArg: 4.118 ± 0.674
3.149AspSer: 3.149 ± 0.462
3.795AspThr: 3.795 ± 0.662
3.068AspVal: 3.068 ± 0.47
0.969AspTrp: 0.969 ± 0.316
2.342AspTyr: 2.342 ± 0.404
0.0AspXaa: 0.0 ± 0.0
Glu
7.509GluAla: 7.509 ± 0.729
0.323GluCys: 0.323 ± 0.199
3.23GluAsp: 3.23 ± 0.569
4.037GluGlu: 4.037 ± 0.643
2.019GluPhe: 2.019 ± 0.43
3.876GluGly: 3.876 ± 0.53
0.807GluHis: 0.807 ± 0.229
1.938GluIle: 1.938 ± 0.338
3.068GluLys: 3.068 ± 0.643
5.006GluLeu: 5.006 ± 0.769
1.534GluMet: 1.534 ± 0.266
2.422GluAsn: 2.422 ± 0.43
1.696GluPro: 1.696 ± 0.486
3.472GluGln: 3.472 ± 0.45
3.068GluArg: 3.068 ± 0.467
2.665GluSer: 2.665 ± 0.558
1.453GluThr: 1.453 ± 0.254
3.956GluVal: 3.956 ± 0.534
1.292GluTrp: 1.292 ± 0.364
2.826GluTyr: 2.826 ± 0.564
0.0GluXaa: 0.0 ± 0.0
Phe
2.826PheAla: 2.826 ± 0.436
0.404PheCys: 0.404 ± 0.206
2.665PheAsp: 2.665 ± 0.506
1.938PheGlu: 1.938 ± 0.531
1.05PhePhe: 1.05 ± 0.351
3.23PheGly: 3.23 ± 0.369
0.727PheHis: 0.727 ± 0.215
1.453PheIle: 1.453 ± 0.361
1.534PheLys: 1.534 ± 0.331
2.18PheLeu: 2.18 ± 0.444
1.453PheMet: 1.453 ± 0.365
1.776PheAsn: 1.776 ± 0.427
1.534PhePro: 1.534 ± 0.345
1.938PheGln: 1.938 ± 0.494
1.776PheArg: 1.776 ± 0.31
2.18PheSer: 2.18 ± 0.4
2.099PheThr: 2.099 ± 0.358
1.292PheVal: 1.292 ± 0.281
0.565PheTrp: 0.565 ± 0.25
0.969PheTyr: 0.969 ± 0.273
0.0PheXaa: 0.0 ± 0.0
Gly
7.186GlyAla: 7.186 ± 1.276
0.404GlyCys: 0.404 ± 0.192
4.602GlyAsp: 4.602 ± 0.622
3.876GlyGlu: 3.876 ± 0.561
2.826GlyPhe: 2.826 ± 0.641
6.136GlyGly: 6.136 ± 0.799
1.857GlyHis: 1.857 ± 0.573
3.633GlyIle: 3.633 ± 0.602
4.602GlyLys: 4.602 ± 0.702
5.168GlyLeu: 5.168 ± 0.55
2.342GlyMet: 2.342 ± 0.42
3.795GlyAsn: 3.795 ± 0.667
2.584GlyPro: 2.584 ± 0.455
3.31GlyGln: 3.31 ± 0.553
5.006GlyArg: 5.006 ± 0.686
5.168GlySer: 5.168 ± 0.794
3.876GlyThr: 3.876 ± 0.628
7.186GlyVal: 7.186 ± 0.692
1.534GlyTrp: 1.534 ± 0.322
2.987GlyTyr: 2.987 ± 0.495
0.0GlyXaa: 0.0 ± 0.0
His
0.969HisAla: 0.969 ± 0.374
0.404HisCys: 0.404 ± 0.271
1.373HisAsp: 1.373 ± 0.306
1.373HisGlu: 1.373 ± 0.305
1.13HisPhe: 1.13 ± 0.324
1.776HisGly: 1.776 ± 0.493
0.646HisHis: 0.646 ± 0.225
0.969HisIle: 0.969 ± 0.266
1.13HisLys: 1.13 ± 0.26
1.615HisLeu: 1.615 ± 0.307
0.646HisMet: 0.646 ± 0.173
0.565HisAsn: 0.565 ± 0.171
1.211HisPro: 1.211 ± 0.33
0.161HisGln: 0.161 ± 0.125
1.453HisArg: 1.453 ± 0.471
1.453HisSer: 1.453 ± 0.405
0.727HisThr: 0.727 ± 0.248
1.292HisVal: 1.292 ± 0.289
0.081HisTrp: 0.081 ± 0.084
0.646HisTyr: 0.646 ± 0.246
0.0HisXaa: 0.0 ± 0.0
Ile
5.652IleAla: 5.652 ± 0.643
0.565IleCys: 0.565 ± 0.204
3.472IleAsp: 3.472 ± 0.481
2.907IleGlu: 2.907 ± 0.463
0.323IlePhe: 0.323 ± 0.147
2.342IleGly: 2.342 ± 0.291
1.05IleHis: 1.05 ± 0.303
1.938IleIle: 1.938 ± 0.354
3.31IleLys: 3.31 ± 0.485
2.422IleLeu: 2.422 ± 0.457
0.646IleMet: 0.646 ± 0.212
2.745IleAsn: 2.745 ± 0.422
2.342IlePro: 2.342 ± 0.447
1.453IleGln: 1.453 ± 0.26
3.068IleArg: 3.068 ± 0.471
1.857IleSer: 1.857 ± 0.404
3.149IleThr: 3.149 ± 0.484
3.149IleVal: 3.149 ± 0.626
0.404IleTrp: 0.404 ± 0.191
1.13IleTyr: 1.13 ± 0.293
0.0IleXaa: 0.0 ± 0.0
Lys
6.782LysAla: 6.782 ± 0.801
0.242LysCys: 0.242 ± 0.125
2.665LysAsp: 2.665 ± 0.318
3.068LysGlu: 3.068 ± 0.609
2.18LysPhe: 2.18 ± 0.296
3.956LysGly: 3.956 ± 0.561
0.969LysHis: 0.969 ± 0.287
1.696LysIle: 1.696 ± 0.333
2.342LysLys: 2.342 ± 0.425
5.571LysLeu: 5.571 ± 0.665
0.807LysMet: 0.807 ± 0.292
2.099LysAsn: 2.099 ± 0.339
2.099LysPro: 2.099 ± 0.444
2.342LysGln: 2.342 ± 0.422
2.503LysArg: 2.503 ± 0.505
2.503LysSer: 2.503 ± 0.412
3.795LysThr: 3.795 ± 0.607
4.037LysVal: 4.037 ± 0.468
1.13LysTrp: 1.13 ± 0.215
1.776LysTyr: 1.776 ± 0.328
0.0LysXaa: 0.0 ± 0.0
Leu
9.931LeuAla: 9.931 ± 0.751
0.807LeuCys: 0.807 ± 0.27
4.845LeuAsp: 4.845 ± 0.708
4.522LeuGlu: 4.522 ± 0.633
2.261LeuPhe: 2.261 ± 0.466
4.199LeuGly: 4.199 ± 0.691
1.776LeuHis: 1.776 ± 0.38
3.633LeuIle: 3.633 ± 0.513
4.36LeuLys: 4.36 ± 0.581
5.813LeuLeu: 5.813 ± 0.893
2.584LeuMet: 2.584 ± 0.412
3.149LeuAsn: 3.149 ± 0.633
3.795LeuPro: 3.795 ± 0.661
2.987LeuGln: 2.987 ± 0.633
5.006LeuArg: 5.006 ± 0.509
4.925LeuSer: 4.925 ± 0.6
4.764LeuThr: 4.764 ± 0.688
3.553LeuVal: 3.553 ± 0.43
1.13LeuTrp: 1.13 ± 0.273
2.826LeuTyr: 2.826 ± 0.447
0.0LeuXaa: 0.0 ± 0.0
Met
4.279MetAla: 4.279 ± 0.751
0.242MetCys: 0.242 ± 0.2
2.261MetAsp: 2.261 ± 0.488
1.292MetGlu: 1.292 ± 0.411
0.565MetPhe: 0.565 ± 0.203
2.18MetGly: 2.18 ± 0.428
0.646MetHis: 0.646 ± 0.211
0.888MetIle: 0.888 ± 0.255
1.373MetLys: 1.373 ± 0.274
3.31MetLeu: 3.31 ± 0.423
0.727MetMet: 0.727 ± 0.202
0.807MetAsn: 0.807 ± 0.214
1.05MetPro: 1.05 ± 0.292
0.565MetGln: 0.565 ± 0.216
2.019MetArg: 2.019 ± 0.301
2.099MetSer: 2.099 ± 0.395
1.696MetThr: 1.696 ± 0.413
0.969MetVal: 0.969 ± 0.294
0.404MetTrp: 0.404 ± 0.174
0.404MetTyr: 0.404 ± 0.208
0.0MetXaa: 0.0 ± 0.0
Asn
5.329AsnAla: 5.329 ± 0.648
0.081AsnCys: 0.081 ± 0.076
2.826AsnAsp: 2.826 ± 0.492
2.261AsnGlu: 2.261 ± 0.547
1.292AsnPhe: 1.292 ± 0.324
3.714AsnGly: 3.714 ± 0.572
0.646AsnHis: 0.646 ± 0.207
2.503AsnIle: 2.503 ± 0.362
2.584AsnLys: 2.584 ± 0.564
3.23AsnLeu: 3.23 ± 0.533
1.05AsnMet: 1.05 ± 0.377
1.534AsnAsn: 1.534 ± 0.399
2.422AsnPro: 2.422 ± 0.677
2.019AsnGln: 2.019 ± 0.32
1.776AsnArg: 1.776 ± 0.367
2.584AsnSer: 2.584 ± 0.508
2.019AsnThr: 2.019 ± 0.489
2.907AsnVal: 2.907 ± 0.488
0.727AsnTrp: 0.727 ± 0.243
1.373AsnTyr: 1.373 ± 0.294
0.0AsnXaa: 0.0 ± 0.0
Pro
4.441ProAla: 4.441 ± 0.711
0.242ProCys: 0.242 ± 0.126
3.068ProAsp: 3.068 ± 0.418
3.23ProGlu: 3.23 ± 0.506
1.615ProPhe: 1.615 ± 0.362
3.795ProGly: 3.795 ± 0.444
1.13ProHis: 1.13 ± 0.359
1.373ProIle: 1.373 ± 0.311
2.342ProLys: 2.342 ± 0.593
3.391ProLeu: 3.391 ± 0.543
0.646ProMet: 0.646 ± 0.225
2.019ProAsn: 2.019 ± 0.475
1.453ProPro: 1.453 ± 0.407
1.938ProGln: 1.938 ± 0.295
1.696ProArg: 1.696 ± 0.481
2.665ProSer: 2.665 ± 0.5
2.907ProThr: 2.907 ± 0.615
2.422ProVal: 2.422 ± 0.381
0.646ProTrp: 0.646 ± 0.202
1.938ProTyr: 1.938 ± 0.359
0.0ProXaa: 0.0 ± 0.0
Gln
5.894GlnAla: 5.894 ± 1.051
0.404GlnCys: 0.404 ± 0.182
1.938GlnAsp: 1.938 ± 0.334
1.938GlnGlu: 1.938 ± 0.411
2.099GlnPhe: 2.099 ± 0.438
3.633GlnGly: 3.633 ± 0.453
0.969GlnHis: 0.969 ± 0.302
1.696GlnIle: 1.696 ± 0.292
1.857GlnLys: 1.857 ± 0.417
3.472GlnLeu: 3.472 ± 0.476
1.292GlnMet: 1.292 ± 0.456
1.615GlnAsn: 1.615 ± 0.347
1.453GlnPro: 1.453 ± 0.461
2.826GlnGln: 2.826 ± 0.645
3.31GlnArg: 3.31 ± 0.538
2.826GlnSer: 2.826 ± 0.446
2.422GlnThr: 2.422 ± 0.441
2.907GlnVal: 2.907 ± 0.488
0.727GlnTrp: 0.727 ± 0.247
1.13GlnTyr: 1.13 ± 0.306
0.0GlnXaa: 0.0 ± 0.0
Arg
6.944ArgAla: 6.944 ± 1.069
0.565ArgCys: 0.565 ± 0.223
3.553ArgAsp: 3.553 ± 0.481
3.553ArgGlu: 3.553 ± 0.445
2.584ArgPhe: 2.584 ± 0.478
3.876ArgGly: 3.876 ± 0.596
0.807ArgHis: 0.807 ± 0.204
3.23ArgIle: 3.23 ± 0.576
2.422ArgLys: 2.422 ± 0.398
4.522ArgLeu: 4.522 ± 0.741
1.776ArgMet: 1.776 ± 0.325
3.31ArgAsn: 3.31 ± 0.54
1.857ArgPro: 1.857 ± 0.409
2.019ArgGln: 2.019 ± 0.555
4.36ArgArg: 4.36 ± 0.656
3.553ArgSer: 3.553 ± 0.71
3.31ArgThr: 3.31 ± 0.739
4.441ArgVal: 4.441 ± 0.549
1.373ArgTrp: 1.373 ± 0.322
2.261ArgTyr: 2.261 ± 0.433
0.0ArgXaa: 0.0 ± 0.0
Ser
6.379SerAla: 6.379 ± 0.661
0.323SerCys: 0.323 ± 0.143
4.36SerAsp: 4.36 ± 0.627
2.745SerGlu: 2.745 ± 0.386
2.019SerPhe: 2.019 ± 0.316
5.248SerGly: 5.248 ± 0.71
0.565SerHis: 0.565 ± 0.332
2.826SerIle: 2.826 ± 0.501
2.987SerLys: 2.987 ± 0.513
4.118SerLeu: 4.118 ± 0.505
2.099SerMet: 2.099 ± 0.362
2.099SerAsn: 2.099 ± 0.552
3.23SerPro: 3.23 ± 0.422
1.938SerGln: 1.938 ± 0.385
3.31SerArg: 3.31 ± 0.554
4.118SerSer: 4.118 ± 0.834
3.795SerThr: 3.795 ± 0.577
2.907SerVal: 2.907 ± 0.516
0.969SerTrp: 0.969 ± 0.286
1.615SerTyr: 1.615 ± 0.398
0.0SerXaa: 0.0 ± 0.0
Thr
7.186ThrAla: 7.186 ± 1.078
0.081ThrCys: 0.081 ± 0.076
3.391ThrAsp: 3.391 ± 0.487
3.472ThrGlu: 3.472 ± 0.713
1.534ThrPhe: 1.534 ± 0.306
4.36ThrGly: 4.36 ± 0.798
1.211ThrHis: 1.211 ± 0.27
2.261ThrIle: 2.261 ± 0.408
3.31ThrLys: 3.31 ± 0.543
4.441ThrLeu: 4.441 ± 0.564
1.13ThrMet: 1.13 ± 0.358
2.422ThrAsn: 2.422 ± 0.39
3.956ThrPro: 3.956 ± 0.534
3.068ThrGln: 3.068 ± 0.427
3.714ThrArg: 3.714 ± 0.601
4.037ThrSer: 4.037 ± 0.593
4.037ThrThr: 4.037 ± 0.595
4.037ThrVal: 4.037 ± 0.757
0.888ThrTrp: 0.888 ± 0.344
1.534ThrTyr: 1.534 ± 0.352
0.0ThrXaa: 0.0 ± 0.0
Val
6.056ValAla: 6.056 ± 0.942
0.484ValCys: 0.484 ± 0.246
5.168ValAsp: 5.168 ± 0.627
3.391ValGlu: 3.391 ± 0.526
2.503ValPhe: 2.503 ± 0.547
4.845ValGly: 4.845 ± 0.508
1.776ValHis: 1.776 ± 0.377
3.149ValIle: 3.149 ± 0.562
3.472ValLys: 3.472 ± 0.44
3.714ValLeu: 3.714 ± 0.508
1.776ValMet: 1.776 ± 0.38
2.584ValAsn: 2.584 ± 0.371
3.149ValPro: 3.149 ± 0.511
3.633ValGln: 3.633 ± 0.648
4.602ValArg: 4.602 ± 0.558
3.31ValSer: 3.31 ± 0.557
4.279ValThr: 4.279 ± 0.535
4.36ValVal: 4.36 ± 0.913
1.292ValTrp: 1.292 ± 0.307
2.019ValTyr: 2.019 ± 0.348
0.0ValXaa: 0.0 ± 0.0
Trp
1.292TrpAla: 1.292 ± 0.384
0.161TrpCys: 0.161 ± 0.119
0.969TrpAsp: 0.969 ± 0.245
1.13TrpGlu: 1.13 ± 0.318
1.05TrpPhe: 1.05 ± 0.265
1.13TrpGly: 1.13 ± 0.332
0.404TrpHis: 0.404 ± 0.182
0.646TrpIle: 0.646 ± 0.192
0.727TrpLys: 0.727 ± 0.241
1.857TrpLeu: 1.857 ± 0.455
0.565TrpMet: 0.565 ± 0.248
0.969TrpAsn: 0.969 ± 0.273
0.484TrpPro: 0.484 ± 0.198
1.05TrpGln: 1.05 ± 0.266
0.484TrpArg: 0.484 ± 0.196
1.13TrpSer: 1.13 ± 0.354
0.969TrpThr: 0.969 ± 0.285
1.373TrpVal: 1.373 ± 0.267
0.161TrpTrp: 0.161 ± 0.107
0.484TrpTyr: 0.484 ± 0.247
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.472TyrAla: 3.472 ± 0.359
0.161TyrCys: 0.161 ± 0.1
1.211TyrAsp: 1.211 ± 0.383
1.453TyrGlu: 1.453 ± 0.314
1.211TyrPhe: 1.211 ± 0.303
2.987TyrGly: 2.987 ± 0.406
0.727TyrHis: 0.727 ± 0.22
0.888TyrIle: 0.888 ± 0.235
1.453TyrLys: 1.453 ± 0.429
2.987TyrLeu: 2.987 ± 0.424
0.565TyrMet: 0.565 ± 0.195
1.615TyrAsn: 1.615 ± 0.317
0.888TyrPro: 0.888 ± 0.213
2.019TyrGln: 2.019 ± 0.353
1.696TyrArg: 1.696 ± 0.275
2.342TyrSer: 2.342 ± 0.43
2.342TyrThr: 2.342 ± 0.502
2.826TyrVal: 2.826 ± 0.566
0.807TyrTrp: 0.807 ± 0.262
0.484TyrTyr: 0.484 ± 0.175
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 41 proteins (12386 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski