Streptococcus satellite phage Javan504
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 26 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZUG8|A0A4D5ZUG8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan504 OX=2558727 GN=JavanS504_0002 PE=4 SV=1
MM1 pKa = 7.55 RR2 pKa = 11.84 TFSDD6 pKa = 3.68 TPKK9 pKa = 10.71 QFMFTYY15 pKa = 9.75 QCKK18 pKa = 10.39 DD19 pKa = 2.96 YY20 pKa = 9.71 DD21 pKa = 4.03 TARR24 pKa = 11.84 VTSTAILGYY33 pKa = 8.7 ITGTYY38 pKa = 7.79 EE39 pKa = 3.86 QNLAEE44 pKa = 4.27 ATLNGDD50 pKa = 3.15 GDD52 pKa = 4.45 LEE54 pKa = 4.24 VTYY57 pKa = 10.96 FEE59 pKa = 5.45 DD60 pKa = 4.58 KK61 pKa = 10.97 SINFNLKK68 pKa = 10.17 RR69 pKa = 11.84 ICDD72 pKa = 3.8 SFKK75 pKa = 10.88 DD76 pKa = 3.81 YY77 pKa = 11.31 CNQPEE82 pKa = 4.1 DD83 pKa = 3.82 MEE85 pKa = 4.59 GEE87 pKa = 4.27 KK88 pKa = 10.75
Molecular weight: 10.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.171
IPC2_protein 4.329
IPC_protein 4.228
Toseland 4.037
ProMoST 4.368
Dawson 4.202
Bjellqvist 4.355
Wikipedia 4.113
Rodwell 4.062
Grimsley 3.948
Solomon 4.19
Lehninger 4.151
Nozaki 4.317
DTASelect 4.507
Thurlkill 4.075
EMBOSS 4.126
Sillero 4.342
Patrickios 2.994
IPC_peptide 4.19
IPC2_peptide 4.329
IPC2.peptide.svr19 4.259
Protein with the highest isoelectric point:
>tr|A0A4D5ZUH1|A0A4D5ZUH1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan504 OX=2558727 GN=JavanS504_0012 PE=4 SV=1
MM1 pKa = 7.57 SGGGAYY7 pKa = 9.43 VCKK10 pKa = 10.2 SQKK13 pKa = 9.01 PKK15 pKa = 9.91 EE16 pKa = 4.06 RR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 QGLSVYY25 pKa = 7.65 EE26 pKa = 4.27 TLTLMIAFGTLIVAIMNNKK45 pKa = 8.89 NKK47 pKa = 10.56
Molecular weight: 5.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.214
IPC2_protein 9.589
IPC_protein 9.692
Toseland 10.365
ProMoST 10.058
Dawson 10.496
Bjellqvist 10.131
Wikipedia 10.628
Rodwell 11.082
Grimsley 10.54
Solomon 10.54
Lehninger 10.526
Nozaki 10.35
DTASelect 10.116
Thurlkill 10.365
EMBOSS 10.745
Sillero 10.409
Patrickios 10.906
IPC_peptide 10.54
IPC2_peptide 8.873
IPC2.peptide.svr19 8.516
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
26
0
26
3585
47
498
137.9
16.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.105 ± 0.419
0.781 ± 0.15
5.969 ± 0.414
8.619 ± 0.594
4.073 ± 0.406
4.714 ± 0.447
1.506 ± 0.201
6.695 ± 0.361
9.372 ± 0.487
10.07 ± 0.465
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.176 ± 0.131
5.802 ± 0.317
2.762 ± 0.483
4.519 ± 0.352
5.077 ± 0.428
5.3 ± 0.281
6.137 ± 0.422
5.384 ± 0.247
1.088 ± 0.2
4.854 ± 0.291
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here