Capybara microvirus Cap1_SP_164
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W5J2|A0A4P8W5J2_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_164 OX=2585400 PE=4 SV=1
MM1 pKa = 7.44 IFKK4 pKa = 8.97 TQFSPHH10 pKa = 5.62 RR11 pKa = 11.84 RR12 pKa = 11.84 YY13 pKa = 9.99 FCNSGMPDD21 pKa = 3.42 ANVYY25 pKa = 10.7 SYY27 pKa = 11.45 VIDD30 pKa = 4.27 PEE32 pKa = 4.47 TGVKK36 pKa = 10.08 DD37 pKa = 3.6 LQITGVRR44 pKa = 11.84 DD45 pKa = 3.65 LQEE48 pKa = 5.19 LIQANYY54 pKa = 10.69 LSGNVALAMQLANNGDD70 pKa = 3.84 DD71 pKa = 4.13 SYY73 pKa = 12.14 LNLVKK78 pKa = 10.56 GFYY81 pKa = 10.77 ADD83 pKa = 3.58 VTDD86 pKa = 4.0 MPSTYY91 pKa = 10.8 AEE93 pKa = 3.78 AVSRR97 pKa = 11.84 VNEE100 pKa = 3.65 AHH102 pKa = 7.3 KK103 pKa = 10.43 IFSQFPPEE111 pKa = 4.22 FKK113 pKa = 10.63 EE114 pKa = 4.36 LFNNSPDD121 pKa = 3.89 EE122 pKa = 4.18 FWSQVGSDD130 pKa = 3.55 EE131 pKa = 4.81 FKK133 pKa = 11.36 AKK135 pKa = 10.66 LFDD138 pKa = 4.05 YY139 pKa = 10.76 DD140 pKa = 5.18 SSDD143 pKa = 3.36 EE144 pKa = 4.21 DD145 pKa = 4.12 LLVAEE150 pKa = 5.39 DD151 pKa = 4.14 PNEE154 pKa = 3.85 EE155 pKa = 4.38 VVDD158 pKa = 4.28 HH159 pKa = 6.3 EE160 pKa = 4.47
Molecular weight: 18.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.139
IPC2_protein 4.113
IPC_protein 4.075
Toseland 3.884
ProMoST 4.19
Dawson 4.05
Bjellqvist 4.24
Wikipedia 3.961
Rodwell 3.91
Grimsley 3.795
Solomon 4.05
Lehninger 3.999
Nozaki 4.164
DTASelect 4.368
Thurlkill 3.923
EMBOSS 3.973
Sillero 4.19
Patrickios 3.249
IPC_peptide 4.05
IPC2_peptide 4.177
IPC2.peptide.svr19 4.106
Protein with the highest isoelectric point:
>tr|A0A4P8W820|A0A4P8W820_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_164 OX=2585400 PE=4 SV=1
MM1 pKa = 7.68 AKK3 pKa = 10.4 LSAADD8 pKa = 3.33 KK9 pKa = 10.78 AYY11 pKa = 10.82 NQTLKK16 pKa = 10.85 DD17 pKa = 3.68 VQTSSNASAEE27 pKa = 4.11 RR28 pKa = 11.84 LQGSAQAFSRR38 pKa = 11.84 EE39 pKa = 3.51 EE40 pKa = 3.94 MAWQKK45 pKa = 10.48 MMSDD49 pKa = 3.44 TSHH52 pKa = 5.79 QRR54 pKa = 11.84 EE55 pKa = 4.04 VADD58 pKa = 4.41 LKK60 pKa = 11.13 KK61 pKa = 10.85 AGLNPVLSANQGATSYY77 pKa = 7.52 TTSGDD82 pKa = 3.52 SGASAAASVLASATSARR99 pKa = 11.84 GTIYY103 pKa = 10.36 SAHH106 pKa = 6.91 ASSEE110 pKa = 4.16 ATKK113 pKa = 10.51 KK114 pKa = 10.84 ASAQSAAATRR124 pKa = 11.84 YY125 pKa = 9.82 AADD128 pKa = 4.34 RR129 pKa = 11.84 NLKK132 pKa = 9.69 AAQTSAAASRR142 pKa = 11.84 YY143 pKa = 9.28 VADD146 pKa = 3.86 QQRR149 pKa = 11.84 AASKK153 pKa = 10.28 YY154 pKa = 8.57 SSDD157 pKa = 3.62 RR158 pKa = 11.84 AKK160 pKa = 10.23 EE161 pKa = 3.75 ASMYY165 pKa = 10.93 SADD168 pKa = 3.55 KK169 pKa = 10.88 AYY171 pKa = 10.39 AAVMNQPRR179 pKa = 11.84 STWASLVDD187 pKa = 3.79 KK188 pKa = 10.61 YY189 pKa = 11.38 GKK191 pKa = 10.02 KK192 pKa = 10.63 LSDD195 pKa = 3.33 ASGLSGFLEE204 pKa = 4.16 KK205 pKa = 10.55 NVKK208 pKa = 9.53 NLSGKK213 pKa = 9.58 IFSFDD218 pKa = 3.26 TSKK221 pKa = 10.04 LTSQNKK227 pKa = 9.5 KK228 pKa = 10.43 DD229 pKa = 3.3 IAKK232 pKa = 10.06 VFVKK236 pKa = 10.74 SFGIANNAKK245 pKa = 10.18 NLTQIYY251 pKa = 9.79 NALAYY256 pKa = 10.78 NNAKK260 pKa = 9.27 MLGKK264 pKa = 10.35 LVFQKK269 pKa = 10.63 AYY271 pKa = 8.72 QPKK274 pKa = 9.19 WSQNPKK280 pKa = 9.46 NKK282 pKa = 9.72 RR283 pKa = 11.84 GKK285 pKa = 10.36 KK286 pKa = 8.84 SS287 pKa = 3.05
Molecular weight: 30.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.088
IPC2_protein 9.487
IPC_protein 9.385
Toseland 10.233
ProMoST 9.809
Dawson 10.379
Bjellqvist 9.999
Wikipedia 10.511
Rodwell 11.038
Grimsley 10.438
Solomon 10.394
Lehninger 10.365
Nozaki 10.189
DTASelect 9.999
Thurlkill 10.248
EMBOSS 10.613
Sillero 10.292
Patrickios 10.716
IPC_peptide 10.394
IPC2_peptide 8.404
IPC2.peptide.svr19 8.51
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1451
83
562
290.2
32.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.994 ± 2.592
1.309 ± 0.521
7.167 ± 0.737
4.273 ± 0.758
4.686 ± 0.638
5.238 ± 0.458
2.136 ± 0.564
4.893 ± 0.853
6.34 ± 1.562
7.236 ± 0.723
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.343 ± 0.273
5.72 ± 0.833
4.48 ± 0.951
3.928 ± 0.569
4.824 ± 0.673
9.511 ± 1.275
5.376 ± 0.398
6.134 ± 0.877
1.516 ± 0.29
4.893 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here