Amino acid dipepetide frequency for Erythrobacter sp. EC-HK427

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.014AlaAla: 18.014 ± 0.221
1.092AlaCys: 1.092 ± 0.04
7.583AlaAsp: 7.583 ± 0.096
8.746AlaGlu: 8.746 ± 0.106
4.58AlaPhe: 4.58 ± 0.077
11.243AlaGly: 11.243 ± 0.13
2.259AlaHis: 2.259 ± 0.061
6.897AlaIle: 6.897 ± 0.111
3.932AlaLys: 3.932 ± 0.092
13.556AlaLeu: 13.556 ± 0.174
4.1AlaMet: 4.1 ± 0.073
3.551AlaAsn: 3.551 ± 0.064
6.119AlaPro: 6.119 ± 0.104
5.061AlaGln: 5.061 ± 0.079
8.88AlaArg: 8.88 ± 0.125
6.592AlaSer: 6.592 ± 0.091
6.081AlaThr: 6.081 ± 0.088
7.989AlaVal: 7.989 ± 0.098
1.515AlaTrp: 1.515 ± 0.047
2.549AlaTyr: 2.549 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
1.078CysAla: 1.078 ± 0.041
0.113CysCys: 0.113 ± 0.012
0.63CysAsp: 0.63 ± 0.031
0.567CysGlu: 0.567 ± 0.033
0.27CysPhe: 0.27 ± 0.02
0.909CysGly: 0.909 ± 0.037
0.199CysHis: 0.199 ± 0.016
0.362CysIle: 0.362 ± 0.019
0.174CysLys: 0.174 ± 0.012
0.668CysLeu: 0.668 ± 0.028
0.147CysMet: 0.147 ± 0.012
0.226CysAsn: 0.226 ± 0.019
0.479CysPro: 0.479 ± 0.026
0.223CysGln: 0.223 ± 0.015
0.551CysArg: 0.551 ± 0.03
0.491CysSer: 0.491 ± 0.024
0.409CysThr: 0.409 ± 0.021
0.57CysVal: 0.57 ± 0.025
0.12CysTrp: 0.12 ± 0.011
0.185CysTyr: 0.185 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
7.926AspAla: 7.926 ± 0.093
0.57AspCys: 0.57 ± 0.029
3.678AspAsp: 3.678 ± 0.146
3.699AspGlu: 3.699 ± 0.063
2.498AspPhe: 2.498 ± 0.067
6.02AspGly: 6.02 ± 0.178
1.159AspHis: 1.159 ± 0.034
3.227AspIle: 3.227 ± 0.078
1.42AspLys: 1.42 ± 0.044
5.69AspLeu: 5.69 ± 0.101
1.475AspMet: 1.475 ± 0.039
1.629AspAsn: 1.629 ± 0.052
3.66AspPro: 3.66 ± 0.068
1.661AspGln: 1.661 ± 0.046
4.117AspArg: 4.117 ± 0.08
2.669AspSer: 2.669 ± 0.059
3.24AspThr: 3.24 ± 0.106
3.99AspVal: 3.99 ± 0.084
1.214AspTrp: 1.214 ± 0.033
1.632AspTyr: 1.632 ± 0.05
0.0AspXaa: 0.0 ± 0.0
Glu
8.673GluAla: 8.673 ± 0.122
0.447GluCys: 0.447 ± 0.022
3.584GluAsp: 3.584 ± 0.066
4.102GluGlu: 4.102 ± 0.094
1.98GluPhe: 1.98 ± 0.056
5.286GluGly: 5.286 ± 0.08
1.287GluHis: 1.287 ± 0.038
3.168GluIle: 3.168 ± 0.066
1.885GluLys: 1.885 ± 0.054
5.742GluLeu: 5.742 ± 0.086
1.668GluMet: 1.668 ± 0.038
1.751GluAsn: 1.751 ± 0.05
2.865GluPro: 2.865 ± 0.065
2.426GluGln: 2.426 ± 0.057
5.227GluArg: 5.227 ± 0.084
2.778GluSer: 2.778 ± 0.054
3.411GluThr: 3.411 ± 0.057
4.034GluVal: 4.034 ± 0.07
0.958GluTrp: 0.958 ± 0.03
1.224GluTyr: 1.224 ± 0.037
0.0GluXaa: 0.0 ± 0.0
Phe
5.462PheAla: 5.462 ± 0.081
0.362PheCys: 0.362 ± 0.019
2.947PheAsp: 2.947 ± 0.071
2.263PheGlu: 2.263 ± 0.055
1.449PhePhe: 1.449 ± 0.049
3.798PheGly: 3.798 ± 0.077
0.725PheHis: 0.725 ± 0.026
1.615PheIle: 1.615 ± 0.043
0.767PheLys: 0.767 ± 0.033
3.185PheLeu: 3.185 ± 0.068
0.774PheMet: 0.774 ± 0.032
1.111PheAsn: 1.111 ± 0.04
1.565PhePro: 1.565 ± 0.039
0.92PheGln: 0.92 ± 0.034
2.145PheArg: 2.145 ± 0.046
2.143PheSer: 2.143 ± 0.05
2.298PheThr: 2.298 ± 0.061
2.705PheVal: 2.705 ± 0.063
0.59PheTrp: 0.59 ± 0.028
0.97PheTyr: 0.97 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
9.775GlyAla: 9.775 ± 0.095
0.844GlyCys: 0.844 ± 0.033
5.435GlyAsp: 5.435 ± 0.199
6.204GlyGlu: 6.204 ± 0.082
3.845GlyPhe: 3.845 ± 0.071
8.506GlyGly: 8.506 ± 0.175
1.697GlyHis: 1.697 ± 0.042
4.419GlyIle: 4.419 ± 0.076
2.985GlyLys: 2.985 ± 0.071
8.335GlyLeu: 8.335 ± 0.097
2.446GlyMet: 2.446 ± 0.052
2.769GlyAsn: 2.769 ± 0.101
3.431GlyPro: 3.431 ± 0.061
3.003GlyGln: 3.003 ± 0.054
5.671GlyArg: 5.671 ± 0.086
4.972GlySer: 4.972 ± 0.091
5.023GlyThr: 5.023 ± 0.13
6.187GlyVal: 6.187 ± 0.083
1.641GlyTrp: 1.641 ± 0.042
2.236GlyTyr: 2.236 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
2.186HisAla: 2.186 ± 0.057
0.265HisCys: 0.265 ± 0.019
1.172HisAsp: 1.172 ± 0.041
1.046HisGlu: 1.046 ± 0.037
0.885HisPhe: 0.885 ± 0.037
1.825HisGly: 1.825 ± 0.051
0.506HisHis: 0.506 ± 0.026
0.979HisIle: 0.979 ± 0.034
0.462HisLys: 0.462 ± 0.025
1.772HisLeu: 1.772 ± 0.045
0.414HisMet: 0.414 ± 0.016
0.476HisAsn: 0.476 ± 0.024
1.265HisPro: 1.265 ± 0.044
0.519HisGln: 0.519 ± 0.026
1.417HisArg: 1.417 ± 0.045
0.962HisSer: 0.962 ± 0.032
0.883HisThr: 0.883 ± 0.029
1.22HisVal: 1.22 ± 0.036
0.346HisTrp: 0.346 ± 0.02
0.596HisTyr: 0.596 ± 0.024
0.0HisXaa: 0.0 ± 0.0
Ile
7.914IleAla: 7.914 ± 0.101
0.459IleCys: 0.459 ± 0.026
3.674IleAsp: 3.674 ± 0.081
3.591IleGlu: 3.591 ± 0.074
1.679IlePhe: 1.679 ± 0.047
5.113IleGly: 5.113 ± 0.079
0.85IleHis: 0.85 ± 0.03
2.216IleIle: 2.216 ± 0.054
1.068IleLys: 1.068 ± 0.039
3.742IleLeu: 3.742 ± 0.065
0.945IleMet: 0.945 ± 0.035
1.371IleAsn: 1.371 ± 0.04
2.359IlePro: 2.359 ± 0.053
1.076IleGln: 1.076 ± 0.034
3.077IleArg: 3.077 ± 0.055
2.614IleSer: 2.614 ± 0.061
3.034IleThr: 3.034 ± 0.082
3.921IleVal: 3.921 ± 0.076
0.626IleTrp: 0.626 ± 0.024
1.053IleTyr: 1.053 ± 0.037
0.0IleXaa: 0.0 ± 0.0
Lys
3.642LysAla: 3.642 ± 0.08
0.157LysCys: 0.157 ± 0.013
1.356LysAsp: 1.356 ± 0.042
1.242LysGlu: 1.242 ± 0.044
0.76LysPhe: 0.76 ± 0.031
2.242LysGly: 2.242 ± 0.056
0.589LysHis: 0.589 ± 0.029
1.307LysIle: 1.307 ± 0.043
1.076LysLys: 1.076 ± 0.051
2.964LysLeu: 2.964 ± 0.071
0.633LysMet: 0.633 ± 0.031
0.637LysAsn: 0.637 ± 0.026
1.64LysPro: 1.64 ± 0.05
0.816LysGln: 0.816 ± 0.031
2.168LysArg: 2.168 ± 0.064
1.451LysSer: 1.451 ± 0.046
1.376LysThr: 1.376 ± 0.038
1.829LysVal: 1.829 ± 0.055
0.33LysTrp: 0.33 ± 0.018
0.524LysTyr: 0.524 ± 0.025
0.0LysXaa: 0.0 ± 0.0
Leu
14.392LeuAla: 14.392 ± 0.162
0.789LeuCys: 0.789 ± 0.034
5.906LeuAsp: 5.906 ± 0.079
5.514LeuGlu: 5.514 ± 0.073
3.611LeuPhe: 3.611 ± 0.06
8.348LeuGly: 8.348 ± 0.116
1.762LeuHis: 1.762 ± 0.043
4.47LeuIle: 4.47 ± 0.081
2.452LeuLys: 2.452 ± 0.057
9.587LeuLeu: 9.587 ± 0.116
2.058LeuMet: 2.058 ± 0.055
2.291LeuAsn: 2.291 ± 0.05
5.462LeuPro: 5.462 ± 0.075
2.739LeuGln: 2.739 ± 0.048
6.695LeuArg: 6.695 ± 0.089
5.599LeuSer: 5.599 ± 0.079
5.556LeuThr: 5.556 ± 0.089
7.329LeuVal: 7.329 ± 0.112
1.186LeuTrp: 1.186 ± 0.037
1.912LeuTyr: 1.912 ± 0.049
0.0LeuXaa: 0.0 ± 0.0
Met
3.4MetAla: 3.4 ± 0.063
0.165MetCys: 0.165 ± 0.013
1.197MetAsp: 1.197 ± 0.037
1.309MetGlu: 1.309 ± 0.044
0.732MetPhe: 0.732 ± 0.027
2.035MetGly: 2.035 ± 0.057
0.452MetHis: 0.452 ± 0.023
1.27MetIle: 1.27 ± 0.037
0.831MetLys: 0.831 ± 0.031
2.667MetLeu: 2.667 ± 0.054
0.585MetMet: 0.585 ± 0.028
0.665MetAsn: 0.665 ± 0.023
1.418MetPro: 1.418 ± 0.044
0.942MetGln: 0.942 ± 0.028
1.856MetArg: 1.856 ± 0.05
1.425MetSer: 1.425 ± 0.035
1.588MetThr: 1.588 ± 0.042
1.637MetVal: 1.637 ± 0.044
0.257MetTrp: 0.257 ± 0.018
0.293MetTyr: 0.293 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
3.58AsnAla: 3.58 ± 0.064
0.27AsnCys: 0.27 ± 0.019
1.649AsnAsp: 1.649 ± 0.104
1.249AsnGlu: 1.249 ± 0.038
1.085AsnPhe: 1.085 ± 0.036
2.632AsnGly: 2.632 ± 0.075
0.488AsnHis: 0.488 ± 0.023
1.37AsnIle: 1.37 ± 0.042
0.551AsnLys: 0.551 ± 0.028
2.53AsnLeu: 2.53 ± 0.048
0.583AsnMet: 0.583 ± 0.022
0.744AsnAsn: 0.744 ± 0.033
1.929AsnPro: 1.929 ± 0.045
0.769AsnGln: 0.769 ± 0.026
1.975AsnArg: 1.975 ± 0.048
1.417AsnSer: 1.417 ± 0.043
1.352AsnThr: 1.352 ± 0.046
2.006AsnVal: 2.006 ± 0.054
0.437AsnTrp: 0.437 ± 0.022
0.655AsnTyr: 0.655 ± 0.029
0.0AsnXaa: 0.0 ± 0.0
Pro
6.778ProAla: 6.778 ± 0.122
0.347ProCys: 0.347 ± 0.02
3.816ProAsp: 3.816 ± 0.07
4.075ProGlu: 4.075 ± 0.08
1.952ProPhe: 1.952 ± 0.045
4.334ProGly: 4.334 ± 0.072
1.035ProHis: 1.035 ± 0.036
2.194ProIle: 2.194 ± 0.044
1.323ProLys: 1.323 ± 0.051
4.929ProLeu: 4.929 ± 0.082
1.16ProMet: 1.16 ± 0.038
1.222ProAsn: 1.222 ± 0.035
2.815ProPro: 2.815 ± 0.074
1.837ProGln: 1.837 ± 0.046
2.777ProArg: 2.777 ± 0.064
2.559ProSer: 2.559 ± 0.052
2.452ProThr: 2.452 ± 0.05
4.239ProVal: 4.239 ± 0.061
0.638ProTrp: 0.638 ± 0.026
1.094ProTyr: 1.094 ± 0.041
0.0ProXaa: 0.0 ± 0.0
Gln
4.103GlnAla: 4.103 ± 0.075
0.246GlnCys: 0.246 ± 0.016
1.629GlnAsp: 1.629 ± 0.052
1.691GlnGlu: 1.691 ± 0.047
1.22GlnPhe: 1.22 ± 0.039
2.587GlnGly: 2.587 ± 0.055
0.663GlnHis: 0.663 ± 0.028
1.835GlnIle: 1.835 ± 0.048
0.735GlnLys: 0.735 ± 0.029
3.371GlnLeu: 3.371 ± 0.059
0.985GlnMet: 0.985 ± 0.032
0.891GlnAsn: 0.891 ± 0.033
1.752GlnPro: 1.752 ± 0.047
1.33GlnGln: 1.33 ± 0.041
2.536GlnArg: 2.536 ± 0.049
1.874GlnSer: 1.874 ± 0.039
1.712GlnThr: 1.712 ± 0.044
2.337GlnVal: 2.337 ± 0.053
0.452GlnTrp: 0.452 ± 0.02
0.712GlnTyr: 0.712 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
7.96ArgAla: 7.96 ± 0.111
0.488ArgCys: 0.488 ± 0.026
4.182ArgAsp: 4.182 ± 0.064
4.648ArgGlu: 4.648 ± 0.084
3.082ArgPhe: 3.082 ± 0.066
4.879ArgGly: 4.879 ± 0.072
1.484ArgHis: 1.484 ± 0.041
4.029ArgIle: 4.029 ± 0.067
2.037ArgLys: 2.037 ± 0.056
7.28ArgLeu: 7.28 ± 0.096
1.897ArgMet: 1.897 ± 0.045
1.929ArgAsn: 1.929 ± 0.045
3.086ArgPro: 3.086 ± 0.064
2.514ArgGln: 2.514 ± 0.052
4.883ArgArg: 4.883 ± 0.098
3.594ArgSer: 3.594 ± 0.07
3.023ArgThr: 3.023 ± 0.058
4.533ArgVal: 4.533 ± 0.082
1.089ArgTrp: 1.089 ± 0.031
1.676ArgTyr: 1.676 ± 0.046
0.0ArgXaa: 0.0 ± 0.0
Ser
6.393SerAla: 6.393 ± 0.085
0.411SerCys: 0.411 ± 0.022
3.25SerAsp: 3.25 ± 0.075
3.182SerGlu: 3.182 ± 0.061
2.193SerPhe: 2.193 ± 0.055
5.831SerGly: 5.831 ± 0.107
0.997SerHis: 0.997 ± 0.026
2.526SerIle: 2.526 ± 0.058
1.272SerLys: 1.272 ± 0.045
5.031SerLeu: 5.031 ± 0.078
1.22SerMet: 1.22 ± 0.038
1.541SerAsn: 1.541 ± 0.037
2.817SerPro: 2.817 ± 0.063
1.647SerGln: 1.647 ± 0.048
3.421SerArg: 3.421 ± 0.068
2.914SerSer: 2.914 ± 0.081
2.727SerThr: 2.727 ± 0.075
3.591SerVal: 3.591 ± 0.066
0.785SerTrp: 0.785 ± 0.028
1.303SerTyr: 1.303 ± 0.037
0.0SerXaa: 0.0 ± 0.0
Thr
6.296ThrAla: 6.296 ± 0.09
0.383ThrCys: 0.383 ± 0.022
3.013ThrAsp: 3.013 ± 0.075
2.579ThrGlu: 2.579 ± 0.051
2.026ThrPhe: 2.026 ± 0.065
5.543ThrGly: 5.543 ± 0.108
0.962ThrHis: 0.962 ± 0.032
3.057ThrIle: 3.057 ± 0.086
1.176ThrLys: 1.176 ± 0.035
5.608ThrLeu: 5.608 ± 0.107
1.219ThrMet: 1.219 ± 0.035
1.387ThrAsn: 1.387 ± 0.048
3.38ThrPro: 3.38 ± 0.069
1.666ThrGln: 1.666 ± 0.042
3.336ThrArg: 3.336 ± 0.062
2.874ThrSer: 2.874 ± 0.093
2.64ThrThr: 2.64 ± 0.075
4.055ThrVal: 4.055 ± 0.073
0.589ThrTrp: 0.589 ± 0.023
1.254ThrTyr: 1.254 ± 0.038
0.0ThrXaa: 0.0 ± 0.0
Val
8.602ValAla: 8.602 ± 0.096
0.561ValCys: 0.561 ± 0.022
4.197ValAsp: 4.197 ± 0.066
4.781ValGlu: 4.781 ± 0.086
2.429ValPhe: 2.429 ± 0.054
5.244ValGly: 5.244 ± 0.078
1.231ValHis: 1.231 ± 0.041
3.93ValIle: 3.93 ± 0.082
1.716ValLys: 1.716 ± 0.052
7.076ValLeu: 7.076 ± 0.096
1.643ValMet: 1.643 ± 0.045
2.003ValAsn: 2.003 ± 0.055
3.809ValPro: 3.809 ± 0.075
2.139ValGln: 2.139 ± 0.049
4.487ValArg: 4.487 ± 0.067
3.97ValSer: 3.97 ± 0.07
4.336ValThr: 4.336 ± 0.083
4.915ValVal: 4.915 ± 0.09
0.956ValTrp: 0.956 ± 0.031
1.336ValTyr: 1.336 ± 0.035
0.0ValXaa: 0.0 ± 0.0
Trp
1.397TrpAla: 1.397 ± 0.035
0.128TrpCys: 0.128 ± 0.012
0.756TrpAsp: 0.756 ± 0.031
0.749TrpGlu: 0.749 ± 0.032
0.614TrpPhe: 0.614 ± 0.025
0.972TrpGly: 0.972 ± 0.035
0.381TrpHis: 0.381 ± 0.019
0.619TrpIle: 0.619 ± 0.023
0.417TrpLys: 0.417 ± 0.022
1.851TrpLeu: 1.851 ± 0.05
0.369TrpMet: 0.369 ± 0.02
0.447TrpAsn: 0.447 ± 0.022
0.709TrpPro: 0.709 ± 0.031
0.692TrpGln: 0.692 ± 0.029
1.283TrpArg: 1.283 ± 0.038
0.88TrpSer: 0.88 ± 0.029
0.709TrpThr: 0.709 ± 0.027
0.833TrpVal: 0.833 ± 0.034
0.285TrpTrp: 0.285 ± 0.02
0.291TrpTyr: 0.291 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.771TyrAla: 2.771 ± 0.057
0.272TyrCys: 0.272 ± 0.017
1.517TyrAsp: 1.517 ± 0.04
1.26TyrGlu: 1.26 ± 0.036
0.905TyrPhe: 0.905 ± 0.039
2.077TyrGly: 2.077 ± 0.053
0.468TyrHis: 0.468 ± 0.024
0.891TyrIle: 0.891 ± 0.031
0.456TyrLys: 0.456 ± 0.024
2.071TyrLeu: 2.071 ± 0.044
0.403TyrMet: 0.403 ± 0.021
0.629TyrAsn: 0.629 ± 0.025
1.018TyrPro: 1.018 ± 0.032
0.683TyrGln: 0.683 ± 0.027
1.79TyrArg: 1.79 ± 0.049
1.277TyrSer: 1.277 ± 0.034
1.195TyrThr: 1.195 ± 0.043
1.442TyrVal: 1.442 ± 0.041
0.369TyrTrp: 0.369 ± 0.023
0.567TyrTyr: 0.567 ± 0.025
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3085 proteins (970700 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski