Circovirus-like genome RW-E

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6GII4|C6GII4_9VIRU Uncharacterized protein OS=Circovirus-like genome RW-E OX=642255 PE=4 SV=1
MM1 pKa = 7.56IRR3 pKa = 11.84RR4 pKa = 11.84QGVYY8 pKa = 8.65WLLTIPEE15 pKa = 4.73DD16 pKa = 3.56EE17 pKa = 5.05FEE19 pKa = 4.05PHH21 pKa = 6.91LPQDD25 pKa = 3.33CAYY28 pKa = 9.97IKK30 pKa = 9.99GQKK33 pKa = 8.96EE34 pKa = 4.04RR35 pKa = 11.84GEE37 pKa = 4.37HH38 pKa = 4.72TGYY41 pKa = 10.82LHH43 pKa = 5.62WQVLVVLRR51 pKa = 11.84RR52 pKa = 11.84KK53 pKa = 10.27GSLSTITGMFGVSCHH68 pKa = 6.66AEE70 pKa = 4.05LSRR73 pKa = 11.84SAAASEE79 pKa = 4.46YY80 pKa = 9.76VWKK83 pKa = 10.46EE84 pKa = 3.62DD85 pKa = 3.86TRR87 pKa = 11.84VGDD90 pKa = 3.42QFEE93 pKa = 4.67FGTLPFKK100 pKa = 11.15RR101 pKa = 11.84NDD103 pKa = 3.53PKK105 pKa = 10.98DD106 pKa = 3.19WEE108 pKa = 4.62QIWEE112 pKa = 4.02RR113 pKa = 11.84AKK115 pKa = 11.04AGDD118 pKa = 3.7INGIPADD125 pKa = 4.02VRR127 pKa = 11.84IQCYY131 pKa = 7.71RR132 pKa = 11.84TLRR135 pKa = 11.84TIRR138 pKa = 11.84ADD140 pKa = 3.57FAVPEE145 pKa = 4.08AMVRR149 pKa = 11.84QCFVYY154 pKa = 10.38CGPTGTGKK162 pKa = 10.01SRR164 pKa = 11.84RR165 pKa = 11.84AWAEE169 pKa = 3.52AGMDD173 pKa = 4.81AYY175 pKa = 10.13PKK177 pKa = 10.5DD178 pKa = 3.81PRR180 pKa = 11.84TKK182 pKa = 9.95FWDD185 pKa = 4.35GYY187 pKa = 10.16RR188 pKa = 11.84DD189 pKa = 3.57QKK191 pKa = 11.1HH192 pKa = 4.69VVVDD196 pKa = 4.05EE197 pKa = 4.0FRR199 pKa = 11.84GAIDD203 pKa = 3.08ISHH206 pKa = 7.45ILRR209 pKa = 11.84WLDD212 pKa = 3.42RR213 pKa = 11.84YY214 pKa = 7.9PTLVEE219 pKa = 3.92IKK221 pKa = 10.65GSATCLVAEE230 pKa = 4.76KK231 pKa = 10.52LWITSNVHH239 pKa = 5.12PNEE242 pKa = 4.31WYY244 pKa = 10.14PMLDD248 pKa = 3.25SATVDD253 pKa = 2.99ALLRR257 pKa = 11.84RR258 pKa = 11.84LQITVFEE265 pKa = 4.42

Molecular weight: 30.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6GII4|C6GII4_9VIRU Uncharacterized protein OS=Circovirus-like genome RW-E OX=642255 PE=4 SV=1
MM1 pKa = 7.63PPIKK5 pKa = 9.84RR6 pKa = 11.84KK7 pKa = 10.32SPSTPKK13 pKa = 9.75SDD15 pKa = 2.86KK16 pKa = 10.15RR17 pKa = 11.84SRR19 pKa = 11.84GSITPGSARR28 pKa = 11.84RR29 pKa = 11.84VLFQARR35 pKa = 11.84VPRR38 pKa = 11.84QRR40 pKa = 11.84SGTNVVTGADD50 pKa = 3.2KK51 pKa = 11.09RR52 pKa = 11.84IGKK55 pKa = 9.07KK56 pKa = 8.75VKK58 pKa = 10.19KK59 pKa = 8.96EE60 pKa = 3.85GRR62 pKa = 11.84VKK64 pKa = 10.52RR65 pKa = 11.84LKK67 pKa = 10.11VSKK70 pKa = 10.64AFRR73 pKa = 11.84KK74 pKa = 9.43KK75 pKa = 10.2VKK77 pKa = 10.21QSLEE81 pKa = 3.98THH83 pKa = 6.96RR84 pKa = 11.84GTGWFRR90 pKa = 11.84EE91 pKa = 4.21TVTIEE96 pKa = 3.97KK97 pKa = 10.16LNCIDD102 pKa = 3.47SQQNVLTPGRR112 pKa = 11.84QVNGTFGNFFSPTYY126 pKa = 8.84IRR128 pKa = 11.84YY129 pKa = 8.79VASHH133 pKa = 7.46LYY135 pKa = 10.43NKK137 pKa = 9.56LAPTQAITVNDD148 pKa = 3.8TNLYY152 pKa = 9.36PVANLKK158 pKa = 10.03IDD160 pKa = 4.09VIEE163 pKa = 3.94QNYY166 pKa = 8.58VMKK169 pKa = 10.69LRR171 pKa = 11.84NNSPRR176 pKa = 11.84TYY178 pKa = 10.99DD179 pKa = 3.35VTLVDD184 pKa = 5.07YY185 pKa = 10.91SPKK188 pKa = 10.31SNNSIATSFEE198 pKa = 4.27DD199 pKa = 3.17TWTSALVNGGPFGAAGTLGQEE220 pKa = 3.94SRR222 pKa = 11.84EE223 pKa = 4.07NVGNATKK230 pKa = 9.57NTMGNHH236 pKa = 6.1PKK238 pKa = 10.14FMAVMRR244 pKa = 11.84HH245 pKa = 5.36YY246 pKa = 11.12YY247 pKa = 11.13SMDD250 pKa = 3.27TVHH253 pKa = 6.75VKK255 pKa = 10.76LEE257 pKa = 4.17PGKK260 pKa = 10.52EE261 pKa = 4.08YY262 pKa = 9.88YY263 pKa = 10.49HH264 pKa = 6.47KK265 pKa = 11.15VKK267 pKa = 9.99GPNMMTYY274 pKa = 11.04DD275 pKa = 3.49FNKK278 pKa = 10.06YY279 pKa = 9.81FKK281 pKa = 10.51DD282 pKa = 3.89SNFHH286 pKa = 7.63DD287 pKa = 3.25IQKK290 pKa = 9.63FCKK293 pKa = 8.58GTLVMCSLDD302 pKa = 3.68LTSTTLATHH311 pKa = 6.49GRR313 pKa = 11.84YY314 pKa = 7.93TDD316 pKa = 3.79IPTADD321 pKa = 3.83PQGMIIEE328 pKa = 4.59TTHH331 pKa = 4.87FTKK334 pKa = 10.49IKK336 pKa = 9.21CPEE339 pKa = 3.78RR340 pKa = 11.84AGFQIPNVAVPIGSIQPLSQQSQNAWSIVYY370 pKa = 9.65PGTAQAGVVEE380 pKa = 4.3YY381 pKa = 10.62RR382 pKa = 11.84QDD384 pKa = 3.71EE385 pKa = 4.48NPLSNVIEE393 pKa = 4.29VV394 pKa = 3.36

Molecular weight: 44.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

659

265

394

329.5

37.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.222 ± 0.788

1.517 ± 0.444

5.159 ± 0.97

5.008 ± 1.06

3.794 ± 0.012

6.829 ± 0.021

2.428 ± 0.127

5.311 ± 0.208

6.829 ± 1.142

5.918 ± 0.744

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.276 ± 0.231

4.552 ± 1.808

5.615 ± 0.421

4.249 ± 0.282

6.98 ± 1.009

5.918 ± 1.05

7.739 ± 1.011

7.739 ± 0.338

1.973 ± 1.07

3.945 ± 0.102
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski