Sanxia sobemo-like virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KER0|A0A1L3KER0_9VIRU RNA-directed RNA polymerase OS=Sanxia sobemo-like virus 3 OX=1923382 PE=4 SV=1
MM1 pKa = 7.15NAVEE5 pKa = 4.54VFSSLRR11 pKa = 11.84WEE13 pKa = 4.36IPDD16 pKa = 4.79DD17 pKa = 3.88FMTYY21 pKa = 7.92SHH23 pKa = 7.71FLRR26 pKa = 11.84VLDD29 pKa = 4.29KK30 pKa = 11.4LDD32 pKa = 3.58MASSPGYY39 pKa = 9.31PYY41 pKa = 9.92MRR43 pKa = 11.84RR44 pKa = 11.84APVNKK49 pKa = 9.19ILFRR53 pKa = 11.84VDD55 pKa = 2.99EE56 pKa = 4.75EE57 pKa = 4.8GAMNPSSVDD66 pKa = 3.68YY67 pKa = 10.48IWQTVSQRR75 pKa = 11.84IAEE78 pKa = 4.5FGDD81 pKa = 3.6ADD83 pKa = 3.71PVRR86 pKa = 11.84FFIKK90 pKa = 10.38QEE92 pKa = 3.58PHH94 pKa = 6.15KK95 pKa = 10.65LKK97 pKa = 10.88KK98 pKa = 10.64LVDD101 pKa = 3.42GRR103 pKa = 11.84NRR105 pKa = 11.84LISSVSVVDD114 pKa = 4.52QIIDD118 pKa = 3.35HH119 pKa = 6.25MLFDD123 pKa = 4.43SMNEE127 pKa = 3.77NMVDD131 pKa = 2.95NWNYY135 pKa = 10.69NPIKK139 pKa = 10.44IGWSPVKK146 pKa = 10.5GGWKK150 pKa = 10.15AFPTGKK156 pKa = 10.16RR157 pKa = 11.84IAIDD161 pKa = 3.22KK162 pKa = 9.97SAWDD166 pKa = 3.48WTVQMWMLEE175 pKa = 3.97LCLTIRR181 pKa = 11.84LALCRR186 pKa = 11.84TDD188 pKa = 4.04GPNRR192 pKa = 11.84DD193 pKa = 3.78LWLKK197 pKa = 9.6IAIYY201 pKa = 9.86RR202 pKa = 11.84YY203 pKa = 9.53KK204 pKa = 10.47QLFLHH209 pKa = 6.59PLFITSGGLLLRR221 pKa = 11.84QQQEE225 pKa = 4.63GVQKK229 pKa = 10.3SGCVNTLADD238 pKa = 3.89NSLMQWILHH247 pKa = 5.71ARR249 pKa = 11.84VCLEE253 pKa = 3.9HH254 pKa = 7.39GVDD257 pKa = 4.7IEE259 pKa = 4.44DD260 pKa = 4.11DD261 pKa = 4.07EE262 pKa = 4.53MWVMGDD268 pKa = 3.92DD269 pKa = 4.19TSQSAVPDD277 pKa = 4.64GYY279 pKa = 11.16EE280 pKa = 3.58DD281 pKa = 3.38WLRR284 pKa = 11.84EE285 pKa = 3.77YY286 pKa = 10.75CIVKK290 pKa = 9.29EE291 pKa = 4.1CQPARR296 pKa = 11.84EE297 pKa = 4.05FCGFRR302 pKa = 11.84FDD304 pKa = 4.98RR305 pKa = 11.84IHH307 pKa = 7.4VEE309 pKa = 3.5PLYY312 pKa = 10.6KK313 pKa = 10.4GKK315 pKa = 9.54HH316 pKa = 5.09AFNLLHH322 pKa = 7.35LDD324 pKa = 3.61EE325 pKa = 5.45KK326 pKa = 11.08NAAEE330 pKa = 4.28VATSYY335 pKa = 11.69ALLYY339 pKa = 10.06HH340 pKa = 7.14RR341 pKa = 11.84SRR343 pKa = 11.84HH344 pKa = 5.5RR345 pKa = 11.84DD346 pKa = 2.42WMRR349 pKa = 11.84NLFEE353 pKa = 5.11SMSLNIFPLWYY364 pKa = 10.16YY365 pKa = 10.65DD366 pKa = 3.98AIYY369 pKa = 10.87DD370 pKa = 4.1GVEE373 pKa = 3.52

Molecular weight: 43.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KER0|A0A1L3KER0_9VIRU RNA-directed RNA polymerase OS=Sanxia sobemo-like virus 3 OX=1923382 PE=4 SV=1
MM1 pKa = 7.26AQNEE5 pKa = 4.41GNRR8 pKa = 11.84TNFLTEE14 pKa = 3.36ISDD17 pKa = 3.53TYY19 pKa = 10.81TRR21 pKa = 11.84ITRR24 pKa = 11.84AASLMVRR31 pKa = 11.84IGIYY35 pKa = 10.05YY36 pKa = 7.67ATAVSFCLLYY46 pKa = 10.86RR47 pKa = 11.84LTRR50 pKa = 11.84WVLSQPVTWTLNAAEE65 pKa = 4.25FVEE68 pKa = 4.64HH69 pKa = 6.11VFYY72 pKa = 10.95GVGTWALWPLDD83 pKa = 3.57KK84 pKa = 11.26GADD87 pKa = 3.14IVVSFLSGPKK97 pKa = 9.59PEE99 pKa = 4.11PTVTEE104 pKa = 4.57RR105 pKa = 11.84IIEE108 pKa = 4.21EE109 pKa = 4.15VNEE112 pKa = 4.02KK113 pKa = 11.0AEE115 pKa = 3.99YY116 pKa = 10.05LYY118 pKa = 11.17SFSAYY123 pKa = 10.2LPSLCYY129 pKa = 9.03ITMVVLIVLLLWVLSRR145 pKa = 11.84RR146 pKa = 11.84YY147 pKa = 10.33LRR149 pKa = 11.84TTLMRR154 pKa = 11.84MRR156 pKa = 11.84GVYY159 pKa = 9.71IGEE162 pKa = 4.14SMRR165 pKa = 11.84NGSKK169 pKa = 10.5FMPAQIPSGQVAVMSAGMLVDD190 pKa = 3.02NHH192 pKa = 6.55IGYY195 pKa = 9.8GLRR198 pKa = 11.84VGNVLVTPYY207 pKa = 10.56HH208 pKa = 6.5VIRR211 pKa = 11.84DD212 pKa = 3.7IVNPILSYY220 pKa = 10.61GGKK223 pKa = 9.74KK224 pKa = 9.17ICVNVAHH231 pKa = 6.31VVASKK236 pKa = 10.15CIPDD240 pKa = 3.59VAYY243 pKa = 11.24LMLDD247 pKa = 3.19EE248 pKa = 4.6TQWTRR253 pKa = 11.84LGVPKK258 pKa = 10.66VKK260 pKa = 10.55VVTKK264 pKa = 5.83TTNVATSVTCVGLSGQSVGLLRR286 pKa = 11.84KK287 pKa = 9.56SSRR290 pKa = 11.84IGMMIYY296 pKa = 10.12SGSTIPGMSGAGYY309 pKa = 9.42FVNNQCHH316 pKa = 5.38GMHH319 pKa = 6.71NGVIMHH325 pKa = 7.03DD326 pKa = 3.54NVGVSMSCIYY336 pKa = 11.12GEE338 pKa = 4.1LKK340 pKa = 10.17MIFRR344 pKa = 11.84GEE346 pKa = 4.05SSHH349 pKa = 7.42DD350 pKa = 3.56FGEE353 pKa = 4.41EE354 pKa = 4.14VVKK357 pKa = 10.8KK358 pKa = 10.25SAGVRR363 pKa = 11.84IFKK366 pKa = 8.78TWSDD370 pKa = 3.49EE371 pKa = 4.18DD372 pKa = 3.85LVKK375 pKa = 10.6QFHH378 pKa = 6.24EE379 pKa = 4.49MWQEE383 pKa = 3.49PDD385 pKa = 3.41FEE387 pKa = 4.71EE388 pKa = 5.87DD389 pKa = 3.88ISGAWGMRR397 pKa = 11.84LGEE400 pKa = 4.07EE401 pKa = 4.22SADD404 pKa = 4.17KK405 pKa = 10.46IIKK408 pKa = 8.65LTGQSPDD415 pKa = 3.41QPEE418 pKa = 3.92MVYY421 pKa = 8.91RR422 pKa = 11.84TTVEE426 pKa = 4.32CKK428 pKa = 10.19SEE430 pKa = 4.03AEE432 pKa = 3.8LRR434 pKa = 11.84EE435 pKa = 4.06RR436 pKa = 11.84IIKK439 pKa = 10.22LEE441 pKa = 3.93QVVEE445 pKa = 3.82ALKK448 pKa = 10.39IEE450 pKa = 4.43KK451 pKa = 9.92CDD453 pKa = 3.16QCSAMFVGVTLAQHH467 pKa = 6.78KK468 pKa = 8.88KK469 pKa = 6.86QHH471 pKa = 6.15SKK473 pKa = 10.58HH474 pKa = 5.85PCISCTNVFGSKK486 pKa = 9.95KK487 pKa = 10.22DD488 pKa = 3.83LEE490 pKa = 4.1QHH492 pKa = 6.17VIKK495 pKa = 9.34THH497 pKa = 4.62VKK499 pKa = 9.99YY500 pKa = 10.54PCDD503 pKa = 3.29HH504 pKa = 6.92CGVVCRR510 pKa = 11.84TEE512 pKa = 4.58VKK514 pKa = 10.36LKK516 pKa = 9.42NHH518 pKa = 6.7RR519 pKa = 11.84EE520 pKa = 4.02SCKK523 pKa = 10.34VSGGGIPMKK532 pKa = 10.36NLKK535 pKa = 10.36GEE537 pKa = 4.12SAFAMDD543 pKa = 3.75HH544 pKa = 5.91KK545 pKa = 10.81KK546 pKa = 9.96IVKK549 pKa = 8.93TDD551 pKa = 3.54PFLGQQRR558 pKa = 11.84TSRR561 pKa = 11.84KK562 pKa = 8.64KK563 pKa = 9.54NGRR566 pKa = 11.84SSEE569 pKa = 4.07RR570 pKa = 11.84TSPAKK575 pKa = 10.07VANHH579 pKa = 6.84RR580 pKa = 11.84SPSQEE585 pKa = 4.14EE586 pKa = 4.08ILCQILQSQKK596 pKa = 10.82NMQQNFEE603 pKa = 4.34KK604 pKa = 10.28FLQVMAGPKK613 pKa = 9.59PATTQNN619 pKa = 3.25

Molecular weight: 69.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

992

373

619

496.0

56.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.343 ± 0.176

2.419 ± 0.333

5.04 ± 1.843

6.048 ± 0.257

3.629 ± 0.57

6.552 ± 0.896

2.923 ± 0.016

5.847 ± 0.032

6.149 ± 0.813

8.165 ± 0.912

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.032 ± 0.007

3.931 ± 0.22

4.032 ± 0.158

3.931 ± 0.274

5.544 ± 0.546

7.157 ± 0.608

4.738 ± 1.263

8.669 ± 1.208

2.319 ± 0.881

3.528 ± 0.303
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski