bacterium HR11

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2436 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5W8S5|A0A2H5W8S5_9BACT Uncharacterized protein OS=bacterium HR11 OX=2035406 GN=HRbin11_01775 PE=4 SV=1
MM1 pKa = 7.17TAVCPEE7 pKa = 4.12CEE9 pKa = 4.24GPIRR13 pKa = 11.84LAEE16 pKa = 4.32DD17 pKa = 3.3LMEE20 pKa = 5.39GEE22 pKa = 5.5IIPCGDD28 pKa = 3.68CGAEE32 pKa = 4.16LEE34 pKa = 4.47VLSVNPLKK42 pKa = 10.93LGLAPEE48 pKa = 4.3VQEE51 pKa = 4.72DD52 pKa = 3.22WGEE55 pKa = 3.77

Molecular weight: 5.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5W9P4|A0A2H5W9P4_9BACT RNA 3'-terminal phosphate cyclase OS=bacterium HR11 OX=2035406 GN=rtcA PE=3 SV=1
MM1 pKa = 7.77PKK3 pKa = 10.64AKK5 pKa = 9.55TKK7 pKa = 10.23RR8 pKa = 11.84AAAKK12 pKa = 9.93RR13 pKa = 11.84FRR15 pKa = 11.84ITPTGKK21 pKa = 9.54VMHH24 pKa = 6.03YY25 pKa = 9.37RR26 pKa = 11.84ASRR29 pKa = 11.84SHH31 pKa = 7.32LLRR34 pKa = 11.84KK35 pKa = 8.61KK36 pKa = 6.54TAKK39 pKa = 9.91RR40 pKa = 11.84KK41 pKa = 8.68RR42 pKa = 11.84HH43 pKa = 5.33LRR45 pKa = 11.84HH46 pKa = 6.52PKK48 pKa = 9.79PMAPGDD54 pKa = 3.53ARR56 pKa = 11.84RR57 pKa = 11.84ARR59 pKa = 11.84RR60 pKa = 11.84MLQASS65 pKa = 3.7

Molecular weight: 7.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2436

0

2436

832185

29

2295

341.6

38.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.044 ± 0.049

0.923 ± 0.02

4.993 ± 0.032

6.173 ± 0.045

3.617 ± 0.031

7.927 ± 0.049

2.104 ± 0.022

4.172 ± 0.038

2.207 ± 0.031

10.863 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.96 ± 0.019

1.721 ± 0.027

6.467 ± 0.039

3.66 ± 0.04

9.476 ± 0.048

4.068 ± 0.036

5.142 ± 0.033

9.12 ± 0.043

2.381 ± 0.032

2.98 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski