Streptococcus phage Javan478

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B7A0|A0A4D6B7A0_9CAUD DNA helicase OS=Streptococcus phage Javan478 OX=2548195 GN=Javan478_0045 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.51NEE4 pKa = 3.97NFLGYY9 pKa = 10.58DD10 pKa = 3.33LAEE13 pKa = 4.29LLEE16 pKa = 4.39EE17 pKa = 4.12KK18 pKa = 10.8LVGKK22 pKa = 9.58RR23 pKa = 11.84LKK25 pKa = 10.98SIDD28 pKa = 3.63DD29 pKa = 3.92EE30 pKa = 5.24KK31 pKa = 11.12IVLADD36 pKa = 3.28GTVIKK41 pKa = 10.6IEE43 pKa = 4.11LNEE46 pKa = 4.36GCGGCGNGWSEE57 pKa = 5.25LNITTEE63 pKa = 4.07NPNLEE68 pKa = 4.3SAVMGVEE75 pKa = 3.92YY76 pKa = 10.18TEE78 pKa = 5.91KK79 pKa = 10.47YY80 pKa = 10.58SEE82 pKa = 3.91WDD84 pKa = 3.42DD85 pKa = 3.44EE86 pKa = 4.73FKK88 pKa = 10.89IFVYY92 pKa = 8.07MTDD95 pKa = 2.96NSVIEE100 pKa = 4.1IYY102 pKa = 10.77GYY104 pKa = 10.9DD105 pKa = 3.49GVGNGYY111 pKa = 9.81YY112 pKa = 10.61GYY114 pKa = 10.67GFWVTVKK121 pKa = 10.85NPGDD125 pKa = 4.03LIEE128 pKa = 5.88SEE130 pKa = 5.33LEE132 pKa = 4.05DD133 pKa = 3.53

Molecular weight: 14.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B5L9|A0A4D6B5L9_9CAUD N6_Mtase domain-containing protein OS=Streptococcus phage Javan478 OX=2548195 GN=Javan478_0034 PE=4 SV=1
MM1 pKa = 7.55NKK3 pKa = 10.03RR4 pKa = 11.84IKK6 pKa = 10.22KK7 pKa = 9.09KK8 pKa = 10.4RR9 pKa = 11.84KK10 pKa = 8.96LEE12 pKa = 3.96TAIVLLIKK20 pKa = 10.27EE21 pKa = 4.31VAEE24 pKa = 3.97LRR26 pKa = 11.84SIVSANAKK34 pKa = 8.06ATNNEE39 pKa = 3.65LAAVKK44 pKa = 10.33SAILDD49 pKa = 3.45NQVAIKK55 pKa = 10.63SIGDD59 pKa = 3.5EE60 pKa = 4.1VGHH63 pKa = 6.84IKK65 pKa = 10.4QNYY68 pKa = 6.67KK69 pKa = 10.51RR70 pKa = 11.84KK71 pKa = 8.22WRR73 pKa = 11.84KK74 pKa = 8.88

Molecular weight: 8.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

12105

42

1307

201.8

22.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.691 ± 0.684

0.512 ± 0.096

6.559 ± 0.307

7.394 ± 0.303

3.924 ± 0.278

6.179 ± 0.385

1.272 ± 0.128

7.08 ± 0.285

9.269 ± 0.351

8.806 ± 0.23

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.371 ± 0.148

5.915 ± 0.216

2.933 ± 0.204

3.767 ± 0.263

4.064 ± 0.214

6.179 ± 0.341

5.898 ± 0.264

6.378 ± 0.268

1.09 ± 0.111

3.717 ± 0.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski