Salmonella phage MG40

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lederbergvirus; unclassified Lederbergvirus

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D7ITS2|A0A7D7ITS2_9CAUD NinF OS=Salmonella phage MG40 OX=2756145 GN=CPT_MG40_057 PE=4 SV=1
MM1 pKa = 7.81KK2 pKa = 10.33LRR4 pKa = 11.84VWHH7 pKa = 6.62IPQVPMKK14 pKa = 9.71PFIVEE19 pKa = 3.96VASVEE24 pKa = 4.04EE25 pKa = 4.66GVRR28 pKa = 11.84VMDD31 pKa = 5.46ALADD35 pKa = 3.83YY36 pKa = 10.99DD37 pKa = 4.43AFQYY41 pKa = 11.41DD42 pKa = 4.1NNIKK46 pKa = 10.06PDD48 pKa = 3.47YY49 pKa = 10.48CNANGLEE56 pKa = 4.09MWDD59 pKa = 4.46EE60 pKa = 4.23SLTDD64 pKa = 3.39QDD66 pKa = 4.38LEE68 pKa = 4.3EE69 pKa = 4.7MEE71 pKa = 4.51LTDD74 pKa = 5.68RR75 pKa = 11.84WVDD78 pKa = 3.24WYY80 pKa = 10.98SEE82 pKa = 4.28CQCYY86 pKa = 10.32DD87 pKa = 3.27DD88 pKa = 3.78PRR90 pKa = 11.84EE91 pKa = 4.19YY92 pKa = 10.25IEE94 pKa = 4.21SLKK97 pKa = 11.19EE98 pKa = 3.87EE99 pKa = 4.34TTTAAA104 pKa = 4.16

Molecular weight: 12.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D7F2B3|A0A7D7F2B3_9CAUD Tailspike protein OS=Salmonella phage MG40 OX=2756145 GN=CPT_MG40_014 PE=4 SV=1
MM1 pKa = 7.39ARR3 pKa = 11.84QRR5 pKa = 11.84RR6 pKa = 11.84SITDD10 pKa = 4.1IICEE14 pKa = 3.96NCKK17 pKa = 10.21YY18 pKa = 10.77LPTKK22 pKa = 10.48RR23 pKa = 11.84SRR25 pKa = 11.84NKK27 pKa = 9.55PKK29 pKa = 10.29PIPKK33 pKa = 9.96EE34 pKa = 3.93SDD36 pKa = 3.19VKK38 pKa = 9.6TFNYY42 pKa = 7.51TAHH45 pKa = 6.73LWDD48 pKa = 3.61IRR50 pKa = 11.84WLRR53 pKa = 11.84EE54 pKa = 3.64RR55 pKa = 11.84ARR57 pKa = 11.84KK58 pKa = 8.87

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

12892

29

725

179.1

20.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.246 ± 0.479

1.311 ± 0.183

5.631 ± 0.232

6.461 ± 0.404

3.343 ± 0.216

7.082 ± 0.383

1.807 ± 0.178

6.058 ± 0.359

6.043 ± 0.321

7.586 ± 0.269

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.676 ± 0.145

4.933 ± 0.267

4.111 ± 0.174

4.778 ± 0.463

5.973 ± 0.27

6.384 ± 0.333

5.5 ± 0.281

6.027 ± 0.26

1.567 ± 0.142

3.483 ± 0.239
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski