Amino acid dipepetide frequency for Salmonella phage MG40

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.464AlaAla: 9.464 ± 1.218
1.551AlaCys: 1.551 ± 0.412
5.43AlaAsp: 5.43 ± 0.842
7.214AlaGlu: 7.214 ± 0.898
3.258AlaPhe: 3.258 ± 0.365
6.361AlaGly: 6.361 ± 0.828
1.241AlaHis: 1.241 ± 0.336
6.516AlaIle: 6.516 ± 0.741
3.956AlaLys: 3.956 ± 0.614
6.982AlaLeu: 6.982 ± 0.913
3.413AlaMet: 3.413 ± 0.588
6.283AlaAsn: 6.283 ± 0.911
2.327AlaPro: 2.327 ± 0.368
3.879AlaGln: 3.879 ± 0.681
5.353AlaArg: 5.353 ± 0.677
5.43AlaSer: 5.43 ± 0.639
5.197AlaThr: 5.197 ± 0.637
5.275AlaVal: 5.275 ± 0.598
1.629AlaTrp: 1.629 ± 0.368
1.707AlaTyr: 1.707 ± 0.379
0.0AlaXaa: 0.0 ± 0.0
Cys
0.698CysAla: 0.698 ± 0.243
0.31CysCys: 0.31 ± 0.163
0.543CysAsp: 0.543 ± 0.23
0.776CysGlu: 0.776 ± 0.269
0.543CysPhe: 0.543 ± 0.187
1.241CysGly: 1.241 ± 0.307
0.388CysHis: 0.388 ± 0.172
0.853CysIle: 0.853 ± 0.289
1.086CysLys: 1.086 ± 0.348
0.853CysLeu: 0.853 ± 0.3
0.155CysMet: 0.155 ± 0.106
0.698CysAsn: 0.698 ± 0.24
0.543CysPro: 0.543 ± 0.185
0.465CysGln: 0.465 ± 0.179
1.319CysArg: 1.319 ± 0.436
0.621CysSer: 0.621 ± 0.248
0.543CysThr: 0.543 ± 0.2
0.853CysVal: 0.853 ± 0.297
0.078CysTrp: 0.078 ± 0.076
0.543CysTyr: 0.543 ± 0.196
0.0CysXaa: 0.0 ± 0.0
Asp
6.516AspAla: 6.516 ± 0.838
0.465AspCys: 0.465 ± 0.182
3.646AspAsp: 3.646 ± 0.571
4.344AspGlu: 4.344 ± 0.516
1.862AspPhe: 1.862 ± 0.408
5.353AspGly: 5.353 ± 0.813
1.241AspHis: 1.241 ± 0.374
4.111AspIle: 4.111 ± 0.47
2.793AspLys: 2.793 ± 0.485
3.879AspLeu: 3.879 ± 0.562
1.086AspMet: 1.086 ± 0.323
2.327AspAsn: 2.327 ± 0.469
1.474AspPro: 1.474 ± 0.34
1.707AspGln: 1.707 ± 0.293
1.784AspArg: 1.784 ± 0.385
2.56AspSer: 2.56 ± 0.417
2.094AspThr: 2.094 ± 0.349
4.887AspVal: 4.887 ± 0.515
1.008AspTrp: 1.008 ± 0.33
3.181AspTyr: 3.181 ± 0.51
0.0AspXaa: 0.0 ± 0.0
Glu
6.439GluAla: 6.439 ± 0.727
1.008GluCys: 1.008 ± 0.283
2.327GluAsp: 2.327 ± 0.396
4.499GluGlu: 4.499 ± 0.888
2.25GluPhe: 2.25 ± 0.417
3.413GluGly: 3.413 ± 0.455
1.164GluHis: 1.164 ± 0.295
3.103GluIle: 3.103 ± 0.544
3.879GluLys: 3.879 ± 0.577
5.818GluLeu: 5.818 ± 0.776
2.948GluMet: 2.948 ± 0.499
2.715GluAsn: 2.715 ± 0.45
2.094GluPro: 2.094 ± 0.372
3.258GluGln: 3.258 ± 0.446
4.267GluArg: 4.267 ± 0.71
3.491GluSer: 3.491 ± 0.438
3.491GluThr: 3.491 ± 0.481
4.267GluVal: 4.267 ± 0.608
1.862GluTrp: 1.862 ± 0.41
2.327GluTyr: 2.327 ± 0.535
0.0GluXaa: 0.0 ± 0.0
Phe
2.715PheAla: 2.715 ± 0.359
0.31PheCys: 0.31 ± 0.151
1.862PheAsp: 1.862 ± 0.439
1.629PheGlu: 1.629 ± 0.311
1.319PhePhe: 1.319 ± 0.382
2.482PheGly: 2.482 ± 0.406
0.776PheHis: 0.776 ± 0.258
2.172PheIle: 2.172 ± 0.448
2.482PheLys: 2.482 ± 0.355
1.784PheLeu: 1.784 ± 0.364
0.931PheMet: 0.931 ± 0.228
1.241PheAsn: 1.241 ± 0.263
1.474PhePro: 1.474 ± 0.354
1.086PheGln: 1.086 ± 0.412
1.629PheArg: 1.629 ± 0.42
2.87PheSer: 2.87 ± 0.437
2.172PheThr: 2.172 ± 0.331
2.094PheVal: 2.094 ± 0.472
0.853PheTrp: 0.853 ± 0.222
1.551PheTyr: 1.551 ± 0.459
0.0PheXaa: 0.0 ± 0.0
Gly
5.818GlyAla: 5.818 ± 0.757
0.698GlyCys: 0.698 ± 0.261
3.181GlyAsp: 3.181 ± 0.494
3.879GlyGlu: 3.879 ± 0.564
2.948GlyPhe: 2.948 ± 0.389
4.732GlyGly: 4.732 ± 0.864
0.931GlyHis: 0.931 ± 0.261
4.732GlyIle: 4.732 ± 0.727
4.81GlyLys: 4.81 ± 0.567
4.577GlyLeu: 4.577 ± 0.617
2.637GlyMet: 2.637 ± 0.543
3.413GlyAsn: 3.413 ± 0.513
1.008GlyPro: 1.008 ± 0.239
3.801GlyGln: 3.801 ± 0.767
4.499GlyArg: 4.499 ± 0.757
4.81GlySer: 4.81 ± 1.022
4.111GlyThr: 4.111 ± 0.706
6.594GlyVal: 6.594 ± 0.745
1.241GlyTrp: 1.241 ± 0.27
2.405GlyTyr: 2.405 ± 0.461
0.0GlyXaa: 0.0 ± 0.0
His
0.776HisAla: 0.776 ± 0.242
0.388HisCys: 0.388 ± 0.179
1.396HisAsp: 1.396 ± 0.351
1.241HisGlu: 1.241 ± 0.342
0.698HisPhe: 0.698 ± 0.191
1.862HisGly: 1.862 ± 0.443
0.543HisHis: 0.543 ± 0.205
0.621HisIle: 0.621 ± 0.168
0.853HisLys: 0.853 ± 0.283
2.25HisLeu: 2.25 ± 0.493
0.388HisMet: 0.388 ± 0.151
0.465HisAsn: 0.465 ± 0.194
1.319HisPro: 1.319 ± 0.284
0.776HisGln: 0.776 ± 0.269
0.931HisArg: 0.931 ± 0.265
0.931HisSer: 0.931 ± 0.288
0.465HisThr: 0.465 ± 0.182
1.008HisVal: 1.008 ± 0.305
0.233HisTrp: 0.233 ± 0.126
0.931HisTyr: 0.931 ± 0.272
0.0HisXaa: 0.0 ± 0.0
Ile
6.128IleAla: 6.128 ± 0.736
0.698IleCys: 0.698 ± 0.219
3.724IleAsp: 3.724 ± 0.629
3.956IleGlu: 3.956 ± 0.522
2.25IlePhe: 2.25 ± 0.596
3.801IleGly: 3.801 ± 0.686
0.776IleHis: 0.776 ± 0.211
4.189IleIle: 4.189 ± 1.015
4.267IleLys: 4.267 ± 0.657
3.568IleLeu: 3.568 ± 0.671
1.164IleMet: 1.164 ± 0.331
2.87IleAsn: 2.87 ± 0.497
2.948IlePro: 2.948 ± 0.532
2.948IleGln: 2.948 ± 0.425
3.413IleArg: 3.413 ± 0.471
5.12IleSer: 5.12 ± 0.737
3.956IleThr: 3.956 ± 0.518
2.948IleVal: 2.948 ± 0.575
0.388IleTrp: 0.388 ± 0.185
1.474IleTyr: 1.474 ± 0.436
0.0IleXaa: 0.0 ± 0.0
Lys
4.81LysAla: 4.81 ± 0.687
1.008LysCys: 1.008 ± 0.316
2.793LysAsp: 2.793 ± 0.499
3.724LysGlu: 3.724 ± 0.586
1.396LysPhe: 1.396 ± 0.366
4.344LysGly: 4.344 ± 0.584
0.931LysHis: 0.931 ± 0.311
3.258LysIle: 3.258 ± 0.368
3.413LysLys: 3.413 ± 0.526
5.43LysLeu: 5.43 ± 0.659
1.319LysMet: 1.319 ± 0.355
2.56LysAsn: 2.56 ± 0.46
3.801LysPro: 3.801 ± 0.628
3.568LysGln: 3.568 ± 0.652
4.267LysArg: 4.267 ± 0.628
4.034LysSer: 4.034 ± 0.582
3.646LysThr: 3.646 ± 0.565
3.103LysVal: 3.103 ± 0.583
0.931LysTrp: 0.931 ± 0.283
2.094LysTyr: 2.094 ± 0.528
0.0LysXaa: 0.0 ± 0.0
Leu
7.602LeuAla: 7.602 ± 0.802
0.853LeuCys: 0.853 ± 0.254
4.034LeuAsp: 4.034 ± 0.655
4.422LeuGlu: 4.422 ± 0.611
1.862LeuPhe: 1.862 ± 0.413
4.654LeuGly: 4.654 ± 0.605
1.707LeuHis: 1.707 ± 0.369
5.12LeuIle: 5.12 ± 0.795
4.887LeuLys: 4.887 ± 0.702
5.973LeuLeu: 5.973 ± 0.725
1.862LeuMet: 1.862 ± 0.372
3.879LeuAsn: 3.879 ± 0.635
3.801LeuPro: 3.801 ± 0.478
3.491LeuGln: 3.491 ± 0.546
5.042LeuArg: 5.042 ± 0.533
5.12LeuSer: 5.12 ± 0.615
5.12LeuThr: 5.12 ± 0.647
3.103LeuVal: 3.103 ± 0.356
1.241LeuTrp: 1.241 ± 0.385
2.094LeuTyr: 2.094 ± 0.396
0.0LeuXaa: 0.0 ± 0.0
Met
3.336MetAla: 3.336 ± 0.471
0.233MetCys: 0.233 ± 0.133
1.164MetAsp: 1.164 ± 0.329
1.629MetGlu: 1.629 ± 0.351
0.543MetPhe: 0.543 ± 0.197
1.707MetGly: 1.707 ± 0.337
0.233MetHis: 0.233 ± 0.122
1.086MetIle: 1.086 ± 0.251
1.939MetLys: 1.939 ± 0.381
1.862MetLeu: 1.862 ± 0.365
0.853MetMet: 0.853 ± 0.28
0.931MetAsn: 0.931 ± 0.321
1.008MetPro: 1.008 ± 0.273
1.474MetGln: 1.474 ± 0.457
2.017MetArg: 2.017 ± 0.427
1.862MetSer: 1.862 ± 0.311
2.172MetThr: 2.172 ± 0.406
1.551MetVal: 1.551 ± 0.287
0.233MetTrp: 0.233 ± 0.137
0.931MetTyr: 0.931 ± 0.265
0.0MetXaa: 0.0 ± 0.0
Asn
5.508AsnAla: 5.508 ± 0.747
0.31AsnCys: 0.31 ± 0.151
3.025AsnAsp: 3.025 ± 0.348
3.103AsnGlu: 3.103 ± 0.468
0.698AsnPhe: 0.698 ± 0.207
3.801AsnGly: 3.801 ± 0.49
1.396AsnHis: 1.396 ± 0.398
2.948AsnIle: 2.948 ± 0.528
2.948AsnLys: 2.948 ± 0.482
4.034AsnLeu: 4.034 ± 0.758
1.396AsnMet: 1.396 ± 0.352
2.405AsnAsn: 2.405 ± 0.444
2.094AsnPro: 2.094 ± 0.362
3.181AsnGln: 3.181 ± 0.55
2.405AsnArg: 2.405 ± 0.49
2.327AsnSer: 2.327 ± 0.41
2.793AsnThr: 2.793 ± 0.398
2.793AsnVal: 2.793 ± 0.734
0.698AsnTrp: 0.698 ± 0.208
1.474AsnTyr: 1.474 ± 0.322
0.0AsnXaa: 0.0 ± 0.0
Pro
3.258ProAla: 3.258 ± 0.429
0.388ProCys: 0.388 ± 0.176
3.336ProAsp: 3.336 ± 0.454
4.344ProGlu: 4.344 ± 0.597
1.396ProPhe: 1.396 ± 0.361
2.094ProGly: 2.094 ± 0.338
0.698ProHis: 0.698 ± 0.243
2.56ProIle: 2.56 ± 0.416
2.715ProLys: 2.715 ± 0.423
2.25ProLeu: 2.25 ± 0.404
0.388ProMet: 0.388 ± 0.181
1.939ProAsn: 1.939 ± 0.343
1.474ProPro: 1.474 ± 0.433
1.784ProGln: 1.784 ± 0.336
2.25ProArg: 2.25 ± 0.435
2.87ProSer: 2.87 ± 0.471
2.327ProThr: 2.327 ± 0.422
3.103ProVal: 3.103 ± 0.517
0.233ProTrp: 0.233 ± 0.129
1.707ProTyr: 1.707 ± 0.414
0.0ProXaa: 0.0 ± 0.0
Gln
4.111GlnAla: 4.111 ± 0.615
0.776GlnCys: 0.776 ± 0.297
2.637GlnAsp: 2.637 ± 0.526
1.862GlnGlu: 1.862 ± 0.383
1.396GlnPhe: 1.396 ± 0.294
3.258GlnGly: 3.258 ± 0.651
0.853GlnHis: 0.853 ± 0.234
3.258GlnIle: 3.258 ± 0.466
2.25GlnLys: 2.25 ± 0.408
4.267GlnLeu: 4.267 ± 0.575
1.396GlnMet: 1.396 ± 0.306
2.87GlnAsn: 2.87 ± 0.759
2.172GlnPro: 2.172 ± 0.424
3.491GlnGln: 3.491 ± 0.864
3.025GlnArg: 3.025 ± 0.426
3.491GlnSer: 3.491 ± 0.528
1.939GlnThr: 1.939 ± 0.423
2.094GlnVal: 2.094 ± 0.428
0.698GlnTrp: 0.698 ± 0.23
1.939GlnTyr: 1.939 ± 0.521
0.0GlnXaa: 0.0 ± 0.0
Arg
4.344ArgAla: 4.344 ± 0.54
0.543ArgCys: 0.543 ± 0.254
3.956ArgAsp: 3.956 ± 0.585
4.654ArgGlu: 4.654 ± 0.606
2.172ArgPhe: 2.172 ± 0.398
3.801ArgGly: 3.801 ± 0.467
1.396ArgHis: 1.396 ± 0.284
3.724ArgIle: 3.724 ± 0.496
4.111ArgLys: 4.111 ± 0.799
4.577ArgLeu: 4.577 ± 0.625
2.017ArgMet: 2.017 ± 0.365
3.336ArgAsn: 3.336 ± 0.557
2.017ArgPro: 2.017 ± 0.363
3.413ArgGln: 3.413 ± 0.514
3.568ArgArg: 3.568 ± 0.739
3.413ArgSer: 3.413 ± 0.423
2.405ArgThr: 2.405 ± 0.431
3.103ArgVal: 3.103 ± 0.455
1.086ArgTrp: 1.086 ± 0.3
2.094ArgTyr: 2.094 ± 0.452
0.0ArgXaa: 0.0 ± 0.0
Ser
5.818SerAla: 5.818 ± 0.928
1.008SerCys: 1.008 ± 0.388
2.948SerAsp: 2.948 ± 0.349
3.801SerGlu: 3.801 ± 0.504
2.405SerPhe: 2.405 ± 0.419
5.74SerGly: 5.74 ± 0.685
1.086SerHis: 1.086 ± 0.347
3.568SerIle: 3.568 ± 0.602
3.724SerLys: 3.724 ± 0.496
4.654SerLeu: 4.654 ± 0.557
1.862SerMet: 1.862 ± 0.245
2.948SerAsn: 2.948 ± 0.577
3.336SerPro: 3.336 ± 0.587
2.715SerGln: 2.715 ± 0.573
4.189SerArg: 4.189 ± 0.581
3.879SerSer: 3.879 ± 0.626
3.181SerThr: 3.181 ± 0.6
3.801SerVal: 3.801 ± 0.606
0.931SerTrp: 0.931 ± 0.279
2.25SerTyr: 2.25 ± 0.556
0.0SerXaa: 0.0 ± 0.0
Thr
5.663ThrAla: 5.663 ± 0.803
0.388ThrCys: 0.388 ± 0.171
2.25ThrAsp: 2.25 ± 0.409
2.87ThrGlu: 2.87 ± 0.531
2.094ThrPhe: 2.094 ± 0.448
4.499ThrGly: 4.499 ± 0.576
0.931ThrHis: 0.931 ± 0.273
3.336ThrIle: 3.336 ± 0.458
4.111ThrLys: 4.111 ± 0.69
4.344ThrLeu: 4.344 ± 0.46
0.853ThrMet: 0.853 ± 0.264
2.482ThrAsn: 2.482 ± 0.422
3.568ThrPro: 3.568 ± 0.539
2.25ThrGln: 2.25 ± 0.33
2.715ThrArg: 2.715 ± 0.483
3.801ThrSer: 3.801 ± 0.54
3.258ThrThr: 3.258 ± 0.523
3.181ThrVal: 3.181 ± 0.546
0.621ThrTrp: 0.621 ± 0.211
1.784ThrTyr: 1.784 ± 0.347
0.0ThrXaa: 0.0 ± 0.0
Val
5.43ValAla: 5.43 ± 0.6
1.164ValCys: 1.164 ± 0.304
3.336ValAsp: 3.336 ± 0.572
4.267ValGlu: 4.267 ± 0.523
2.094ValPhe: 2.094 ± 0.431
4.422ValGly: 4.422 ± 0.68
0.543ValHis: 0.543 ± 0.215
3.258ValIle: 3.258 ± 0.627
3.568ValLys: 3.568 ± 0.5
4.577ValLeu: 4.577 ± 0.586
1.241ValMet: 1.241 ± 0.382
3.801ValAsn: 3.801 ± 0.7
2.25ValPro: 2.25 ± 0.527
2.405ValGln: 2.405 ± 0.378
2.87ValArg: 2.87 ± 0.545
4.267ValSer: 4.267 ± 0.705
3.879ValThr: 3.879 ± 0.576
3.724ValVal: 3.724 ± 0.494
1.086ValTrp: 1.086 ± 0.288
2.094ValTyr: 2.094 ± 0.449
0.0ValXaa: 0.0 ± 0.0
Trp
1.241TrpAla: 1.241 ± 0.268
0.388TrpCys: 0.388 ± 0.159
1.629TrpAsp: 1.629 ± 0.335
0.621TrpGlu: 0.621 ± 0.239
0.621TrpPhe: 0.621 ± 0.212
1.164TrpGly: 1.164 ± 0.276
0.698TrpHis: 0.698 ± 0.246
0.621TrpIle: 0.621 ± 0.195
1.008TrpLys: 1.008 ± 0.337
1.707TrpLeu: 1.707 ± 0.52
0.155TrpMet: 0.155 ± 0.121
0.543TrpAsn: 0.543 ± 0.19
0.698TrpPro: 0.698 ± 0.224
0.621TrpGln: 0.621 ± 0.186
1.008TrpArg: 1.008 ± 0.271
0.853TrpSer: 0.853 ± 0.317
0.388TrpThr: 0.388 ± 0.145
0.853TrpVal: 0.853 ± 0.219
0.155TrpTrp: 0.155 ± 0.098
0.698TrpTyr: 0.698 ± 0.237
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.793TyrAla: 2.793 ± 0.453
0.621TyrCys: 0.621 ± 0.274
3.103TyrAsp: 3.103 ± 0.585
1.784TyrGlu: 1.784 ± 0.412
1.629TyrPhe: 1.629 ± 0.379
2.094TyrGly: 2.094 ± 0.519
0.543TyrHis: 0.543 ± 0.206
1.551TyrIle: 1.551 ± 0.469
1.629TyrLys: 1.629 ± 0.304
2.482TyrLeu: 2.482 ± 0.441
0.621TyrMet: 0.621 ± 0.211
1.629TyrAsn: 1.629 ± 0.448
1.707TyrPro: 1.707 ± 0.494
1.474TyrGln: 1.474 ± 0.293
3.181TyrArg: 3.181 ± 0.499
2.094TyrSer: 2.094 ± 0.431
1.862TyrThr: 1.862 ± 0.3
1.939TyrVal: 1.939 ± 0.393
0.465TyrTrp: 0.465 ± 0.176
1.551TyrTyr: 1.551 ± 0.376
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 72 proteins (12892 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski