Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3263 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6FAG4|Q6FAG4_ACIAD Uncharacterized protein OS=Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) OX=62977 GN=ACIAD2146 PE=4 SV=1
MM1 pKa = 6.5 YY2 pKa = 8.99 THH4 pKa = 7.47 LVLTLGKK11 pKa = 10.48 DD12 pKa = 3.26 YY13 pKa = 11.16 DD14 pKa = 4.09 YY15 pKa = 11.81 VLNEE19 pKa = 3.85 LDD21 pKa = 3.83 FPRR24 pKa = 11.84 VSALNAYY31 pKa = 8.31 QKK33 pKa = 10.15 QYY35 pKa = 10.73 PPAEE39 pKa = 4.0 VGIQRR44 pKa = 11.84 LCRR47 pKa = 11.84 ILEE50 pKa = 4.03 AFMGIEE56 pKa = 4.64 DD57 pKa = 4.48 SPSFDD62 pKa = 5.94 DD63 pKa = 6.08 IEE65 pKa = 6.18 SDD67 pKa = 4.64 DD68 pKa = 4.97 DD69 pKa = 5.3 NMMDD73 pKa = 5.0 DD74 pKa = 5.74 LMNFPQGGG82 pKa = 3.33
Molecular weight: 9.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.846
IPC_protein 3.808
Toseland 3.592
ProMoST 3.999
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.783
Rodwell 3.643
Grimsley 3.503
Solomon 3.795
Lehninger 3.757
Nozaki 3.935
DTASelect 4.19
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.935
Patrickios 1.901
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.834
Protein with the highest isoelectric point:
>tr|Q6F6Q4|Q6F6Q4_ACIAD Putative transcriptional regulator OS=Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) OX=62977 GN=ACIAD3629 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.55 RR3 pKa = 11.84 TFQPSEE9 pKa = 3.97 LKK11 pKa = 10.13 RR12 pKa = 11.84 KK13 pKa = 8.98 RR14 pKa = 11.84 VHH16 pKa = 6.36 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 AGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.18 GRR39 pKa = 11.84 HH40 pKa = 5.1 SLTVV44 pKa = 3.06
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.402
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3263
0
3263
1044193
23
3711
320.0
35.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.451 ± 0.046
0.95 ± 0.014
5.191 ± 0.03
5.558 ± 0.039
4.301 ± 0.039
6.596 ± 0.047
2.555 ± 0.027
7.043 ± 0.037
5.29 ± 0.036
10.55 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.514 ± 0.023
4.341 ± 0.041
3.969 ± 0.023
5.612 ± 0.048
4.385 ± 0.036
6.237 ± 0.034
5.351 ± 0.038
6.555 ± 0.033
1.276 ± 0.016
3.276 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here