Escherichia phage vB_EcoM_VR7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Gaprivervirus; Escherichia virus VR7

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 293 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5FIR0|E5FIR0_9CAUD Gp55 RNA polymerase sigma factor OS=Escherichia phage vB_EcoM_VR7 OX=700939 GN=55 PE=4 SV=1
MM1 pKa = 7.8RR2 pKa = 11.84LYY4 pKa = 10.85DD5 pKa = 3.98FNEE8 pKa = 4.02ACLEE12 pKa = 4.25HH13 pKa = 8.15IRR15 pKa = 11.84EE16 pKa = 4.36CDD18 pKa = 3.83VVDD21 pKa = 6.32DD22 pKa = 4.97DD23 pKa = 5.14NQPNTIKK30 pKa = 10.67YY31 pKa = 8.09WFEE34 pKa = 4.98AITDD38 pKa = 3.95RR39 pKa = 11.84EE40 pKa = 4.22TGQLIKK46 pKa = 10.89VYY48 pKa = 10.64VVTDD52 pKa = 3.6WAVEE56 pKa = 4.0FSFNVMEE63 pKa = 5.94DD64 pKa = 3.71DD65 pKa = 3.78TPEE68 pKa = 4.19TLIHH72 pKa = 5.85MAEE75 pKa = 4.12SCIKK79 pKa = 10.31DD80 pKa = 3.28AYY82 pKa = 10.2YY83 pKa = 10.93DD84 pKa = 3.64EE85 pKa = 5.92DD86 pKa = 3.8FF87 pKa = 4.89

Molecular weight: 10.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E5FIS8|E5FIS8_9CAUD Uncharacterized protein 49.1 OS=Escherichia phage vB_EcoM_VR7 OX=700939 GN=49.1 PE=4 SV=1
MM1 pKa = 7.05KK2 pKa = 9.75TVLVYY7 pKa = 9.03TEE9 pKa = 4.46KK10 pKa = 11.26LNGGKK15 pKa = 8.33VWKK18 pKa = 10.04LLIKK22 pKa = 10.6GSPTDD27 pKa = 3.52RR28 pKa = 11.84FMVTCVGLYY37 pKa = 7.88TRR39 pKa = 11.84PTKK42 pKa = 10.87KK43 pKa = 9.69MIRR46 pKa = 11.84QFKK49 pKa = 8.73RR50 pKa = 11.84LHH52 pKa = 5.81RR53 pKa = 11.84SFYY56 pKa = 10.96NII58 pKa = 3.6

Molecular weight: 6.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

293

0

293

53667

29

1291

183.2

20.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.161 ± 0.166

1.116 ± 0.076

6.194 ± 0.112

7.196 ± 0.166

4.414 ± 0.113

6.335 ± 0.238

1.809 ± 0.088

6.844 ± 0.098

7.496 ± 0.211

7.455 ± 0.149

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.814 ± 0.101

5.305 ± 0.131

3.615 ± 0.096

3.764 ± 0.113

4.269 ± 0.098

5.905 ± 0.168

6.041 ± 0.226

6.596 ± 0.126

1.444 ± 0.061

4.226 ± 0.108
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski