Amino acid dipepetide frequency for Escherichia phage vB_EcoM_VR7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.683AlaAla: 5.683 ± 0.418
0.596AlaCys: 0.596 ± 0.1
4.193AlaAsp: 4.193 ± 0.239
5.255AlaGlu: 5.255 ± 0.418
2.683AlaPhe: 2.683 ± 0.202
5.236AlaGly: 5.236 ± 0.389
1.267AlaHis: 1.267 ± 0.161
5.106AlaIle: 5.106 ± 0.315
4.994AlaLys: 4.994 ± 0.327
5.758AlaLeu: 5.758 ± 0.351
1.807AlaMet: 1.807 ± 0.169
3.149AlaAsn: 3.149 ± 0.229
2.702AlaPro: 2.702 ± 0.192
2.702AlaGln: 2.702 ± 0.215
3.149AlaArg: 3.149 ± 0.198
4.453AlaSer: 4.453 ± 0.334
4.453AlaThr: 4.453 ± 0.482
4.677AlaVal: 4.677 ± 0.349
1.099AlaTrp: 1.099 ± 0.123
2.646AlaTyr: 2.646 ± 0.201
0.0AlaXaa: 0.0 ± 0.0
Cys
0.839CysAla: 0.839 ± 0.132
0.186CysCys: 0.186 ± 0.055
0.894CysAsp: 0.894 ± 0.124
0.894CysGlu: 0.894 ± 0.145
0.391CysPhe: 0.391 ± 0.096
0.876CysGly: 0.876 ± 0.118
0.373CysHis: 0.373 ± 0.089
0.596CysIle: 0.596 ± 0.119
0.82CysLys: 0.82 ± 0.133
0.708CysLeu: 0.708 ± 0.116
0.317CysMet: 0.317 ± 0.081
0.373CysAsn: 0.373 ± 0.089
0.503CysPro: 0.503 ± 0.114
0.503CysGln: 0.503 ± 0.091
0.484CysArg: 0.484 ± 0.089
0.652CysSer: 0.652 ± 0.12
0.503CysThr: 0.503 ± 0.123
0.689CysVal: 0.689 ± 0.104
0.13CysTrp: 0.13 ± 0.052
0.429CysTyr: 0.429 ± 0.093
0.0CysXaa: 0.0 ± 0.0
Asp
4.435AspAla: 4.435 ± 0.334
0.708AspCys: 0.708 ± 0.119
3.839AspAsp: 3.839 ± 0.322
4.603AspGlu: 4.603 ± 0.325
3.112AspPhe: 3.112 ± 0.247
4.826AspGly: 4.826 ± 0.332
0.801AspHis: 0.801 ± 0.12
4.304AspIle: 4.304 ± 0.293
4.696AspLys: 4.696 ± 0.344
4.696AspLeu: 4.696 ± 0.283
1.901AspMet: 1.901 ± 0.209
2.963AspAsn: 2.963 ± 0.224
2.031AspPro: 2.031 ± 0.217
1.975AspGln: 1.975 ± 0.192
2.012AspArg: 2.012 ± 0.188
3.335AspSer: 3.335 ± 0.262
3.112AspThr: 3.112 ± 0.253
4.453AspVal: 4.453 ± 0.333
1.099AspTrp: 1.099 ± 0.142
3.019AspTyr: 3.019 ± 0.245
0.0AspXaa: 0.0 ± 0.0
Glu
6.168GluAla: 6.168 ± 0.453
1.025GluCys: 1.025 ± 0.155
3.95GluAsp: 3.95 ± 0.264
4.863GluGlu: 4.863 ± 0.343
3.54GluPhe: 3.54 ± 0.266
3.801GluGly: 3.801 ± 0.275
1.248GluHis: 1.248 ± 0.156
5.553GluIle: 5.553 ± 0.347
4.155GluLys: 4.155 ± 0.344
6.447GluLeu: 6.447 ± 0.369
2.199GluMet: 2.199 ± 0.208
3.447GluAsn: 3.447 ± 0.225
2.106GluPro: 2.106 ± 0.195
2.814GluGln: 2.814 ± 0.255
2.814GluArg: 2.814 ± 0.227
4.044GluSer: 4.044 ± 0.263
4.621GluThr: 4.621 ± 0.297
4.752GluVal: 4.752 ± 0.258
1.043GluTrp: 1.043 ± 0.113
3.373GluTyr: 3.373 ± 0.304
0.0GluXaa: 0.0 ± 0.0
Phe
2.907PheAla: 2.907 ± 0.232
0.447PheCys: 0.447 ± 0.081
3.447PheAsp: 3.447 ± 0.273
3.391PheGlu: 3.391 ± 0.285
1.509PhePhe: 1.509 ± 0.187
3.0PheGly: 3.0 ± 0.266
0.578PheHis: 0.578 ± 0.095
2.926PheIle: 2.926 ± 0.235
3.745PheLys: 3.745 ± 0.292
2.571PheLeu: 2.571 ± 0.208
1.398PheMet: 1.398 ± 0.162
2.888PheAsn: 2.888 ± 0.237
1.248PhePro: 1.248 ± 0.167
1.416PheGln: 1.416 ± 0.168
2.068PheArg: 2.068 ± 0.177
2.404PheSer: 2.404 ± 0.213
2.478PheThr: 2.478 ± 0.203
3.0PheVal: 3.0 ± 0.281
0.764PheTrp: 0.764 ± 0.129
1.957PheTyr: 1.957 ± 0.201
0.0PheXaa: 0.0 ± 0.0
Gly
3.913GlyAla: 3.913 ± 0.399
0.689GlyCys: 0.689 ± 0.117
4.081GlyAsp: 4.081 ± 0.419
4.062GlyGlu: 4.062 ± 0.291
2.832GlyPhe: 2.832 ± 0.263
3.764GlyGly: 3.764 ± 0.323
0.969GlyHis: 0.969 ± 0.14
4.584GlyIle: 4.584 ± 0.396
4.267GlyLys: 4.267 ± 0.326
5.217GlyLeu: 5.217 ± 0.32
1.807GlyMet: 1.807 ± 0.221
3.205GlyAsn: 3.205 ± 0.427
2.273GlyPro: 2.273 ± 0.413
2.497GlyGln: 2.497 ± 0.241
2.59GlyArg: 2.59 ± 0.229
3.913GlySer: 3.913 ± 0.251
4.081GlyThr: 4.081 ± 0.432
4.435GlyVal: 4.435 ± 0.268
1.155GlyTrp: 1.155 ± 0.136
3.019GlyTyr: 3.019 ± 0.211
0.0GlyXaa: 0.0 ± 0.0
His
1.025HisAla: 1.025 ± 0.133
0.205HisCys: 0.205 ± 0.074
1.174HisAsp: 1.174 ± 0.139
1.36HisGlu: 1.36 ± 0.175
0.82HisPhe: 0.82 ± 0.131
1.118HisGly: 1.118 ± 0.146
0.391HisHis: 0.391 ± 0.089
1.379HisIle: 1.379 ± 0.19
1.416HisLys: 1.416 ± 0.168
1.379HisLeu: 1.379 ± 0.143
0.54HisMet: 0.54 ± 0.112
0.857HisAsn: 0.857 ± 0.144
1.006HisPro: 1.006 ± 0.115
0.615HisGln: 0.615 ± 0.105
0.801HisArg: 0.801 ± 0.12
0.839HisSer: 0.839 ± 0.12
1.006HisThr: 1.006 ± 0.18
1.155HisVal: 1.155 ± 0.142
0.28HisTrp: 0.28 ± 0.074
0.727HisTyr: 0.727 ± 0.119
0.0HisXaa: 0.0 ± 0.0
Ile
5.087IleAla: 5.087 ± 0.372
0.764IleCys: 0.764 ± 0.137
4.696IleAsp: 4.696 ± 0.266
5.217IleGlu: 5.217 ± 0.317
2.348IlePhe: 2.348 ± 0.209
3.522IleGly: 3.522 ± 0.234
1.174IleHis: 1.174 ± 0.159
3.894IleIle: 3.894 ± 0.265
6.224IleLys: 6.224 ± 0.445
3.615IleLeu: 3.615 ± 0.249
2.143IleMet: 2.143 ± 0.192
4.211IleAsn: 4.211 ± 0.274
2.683IlePro: 2.683 ± 0.198
3.186IleGln: 3.186 ± 0.338
3.335IleArg: 3.335 ± 0.23
4.174IleSer: 4.174 ± 0.254
4.491IleThr: 4.491 ± 0.319
4.565IleVal: 4.565 ± 0.294
0.596IleTrp: 0.596 ± 0.099
2.516IleTyr: 2.516 ± 0.205
0.0IleXaa: 0.0 ± 0.0
Lys
5.758LysAla: 5.758 ± 0.37
0.578LysCys: 0.578 ± 0.099
4.453LysAsp: 4.453 ± 0.269
5.87LysGlu: 5.87 ± 0.345
3.391LysPhe: 3.391 ± 0.269
4.547LysGly: 4.547 ± 0.355
1.603LysHis: 1.603 ± 0.18
4.957LysIle: 4.957 ± 0.335
4.658LysLys: 4.658 ± 0.299
6.056LysLeu: 6.056 ± 0.341
2.329LysMet: 2.329 ± 0.207
3.839LysAsn: 3.839 ± 0.313
2.87LysPro: 2.87 ± 0.233
2.627LysGln: 2.627 ± 0.24
3.559LysArg: 3.559 ± 0.264
4.435LysSer: 4.435 ± 0.398
4.211LysThr: 4.211 ± 0.271
5.068LysVal: 5.068 ± 0.355
1.155LysTrp: 1.155 ± 0.139
3.0LysTyr: 3.0 ± 0.239
0.0LysXaa: 0.0 ± 0.0
Leu
5.143LeuAla: 5.143 ± 0.319
0.708LeuCys: 0.708 ± 0.143
4.826LeuAsp: 4.826 ± 0.268
5.367LeuGlu: 5.367 ± 0.37
3.391LeuPhe: 3.391 ± 0.261
3.95LeuGly: 3.95 ± 0.254
1.211LeuHis: 1.211 ± 0.143
4.994LeuIle: 4.994 ± 0.279
6.522LeuLys: 6.522 ± 0.382
5.124LeuLeu: 5.124 ± 0.339
2.18LeuMet: 2.18 ± 0.203
4.808LeuAsn: 4.808 ± 0.27
2.944LeuPro: 2.944 ± 0.241
2.739LeuGln: 2.739 ± 0.232
3.093LeuArg: 3.093 ± 0.192
4.435LeuSer: 4.435 ± 0.259
4.714LeuThr: 4.714 ± 0.294
4.603LeuVal: 4.603 ± 0.32
0.727LeuTrp: 0.727 ± 0.117
3.075LeuTyr: 3.075 ± 0.266
0.0LeuXaa: 0.0 ± 0.0
Met
2.143MetAla: 2.143 ± 0.22
0.391MetCys: 0.391 ± 0.082
1.398MetAsp: 1.398 ± 0.178
1.714MetGlu: 1.714 ± 0.17
1.472MetPhe: 1.472 ± 0.183
1.603MetGly: 1.603 ± 0.175
0.466MetHis: 0.466 ± 0.104
1.957MetIle: 1.957 ± 0.195
2.571MetLys: 2.571 ± 0.244
1.994MetLeu: 1.994 ± 0.172
0.82MetMet: 0.82 ± 0.112
1.845MetAsn: 1.845 ± 0.187
0.708MetPro: 0.708 ± 0.104
1.174MetGln: 1.174 ± 0.138
1.043MetArg: 1.043 ± 0.121
2.012MetSer: 2.012 ± 0.205
1.975MetThr: 1.975 ± 0.19
1.64MetVal: 1.64 ± 0.179
0.224MetTrp: 0.224 ± 0.069
0.988MetTyr: 0.988 ± 0.166
0.0MetXaa: 0.0 ± 0.0
Asn
3.671AsnAla: 3.671 ± 0.262
0.578AsnCys: 0.578 ± 0.117
2.851AsnAsp: 2.851 ± 0.208
3.689AsnGlu: 3.689 ± 0.289
2.385AsnPhe: 2.385 ± 0.21
3.839AsnGly: 3.839 ± 0.304
1.137AsnHis: 1.137 ± 0.162
3.82AsnIle: 3.82 ± 0.203
4.081AsnLys: 4.081 ± 0.243
3.857AsnLeu: 3.857 ± 0.273
1.398AsnMet: 1.398 ± 0.201
3.075AsnAsn: 3.075 ± 0.247
2.087AsnPro: 2.087 ± 0.26
1.975AsnGln: 1.975 ± 0.186
2.385AsnArg: 2.385 ± 0.203
2.963AsnSer: 2.963 ± 0.247
3.112AsnThr: 3.112 ± 0.288
3.168AsnVal: 3.168 ± 0.267
0.634AsnTrp: 0.634 ± 0.107
2.348AsnTyr: 2.348 ± 0.209
0.0AsnXaa: 0.0 ± 0.0
Pro
2.422ProAla: 2.422 ± 0.214
0.317ProCys: 0.317 ± 0.075
2.721ProAsp: 2.721 ± 0.221
3.41ProGlu: 3.41 ± 0.242
1.416ProPhe: 1.416 ± 0.181
2.59ProGly: 2.59 ± 0.232
0.671ProHis: 0.671 ± 0.122
1.938ProIle: 1.938 ± 0.192
2.422ProLys: 2.422 ± 0.216
2.497ProLeu: 2.497 ± 0.178
0.634ProMet: 0.634 ± 0.1
1.64ProAsn: 1.64 ± 0.175
0.969ProPro: 0.969 ± 0.146
1.006ProGln: 1.006 ± 0.179
1.193ProArg: 1.193 ± 0.149
2.106ProSer: 2.106 ± 0.201
2.665ProThr: 2.665 ± 0.231
3.317ProVal: 3.317 ± 0.247
0.689ProTrp: 0.689 ± 0.111
1.528ProTyr: 1.528 ± 0.191
0.0ProXaa: 0.0 ± 0.0
Gln
2.758GlnAla: 2.758 ± 0.243
0.335GlnCys: 0.335 ± 0.082
1.901GlnAsp: 1.901 ± 0.186
2.609GlnGlu: 2.609 ± 0.243
1.789GlnPhe: 1.789 ± 0.172
2.851GlnGly: 2.851 ± 0.658
0.596GlnHis: 0.596 ± 0.115
3.075GlnIle: 3.075 ± 0.248
2.46GlnLys: 2.46 ± 0.199
3.168GlnLeu: 3.168 ± 0.235
1.025GlnMet: 1.025 ± 0.136
1.603GlnAsn: 1.603 ± 0.17
1.137GlnPro: 1.137 ± 0.161
1.174GlnGln: 1.174 ± 0.167
1.863GlnArg: 1.863 ± 0.169
1.975GlnSer: 1.975 ± 0.183
2.739GlnThr: 2.739 ± 0.214
2.143GlnVal: 2.143 ± 0.176
0.727GlnTrp: 0.727 ± 0.12
1.714GlnTyr: 1.714 ± 0.207
0.0GlnXaa: 0.0 ± 0.0
Arg
3.112ArgAla: 3.112 ± 0.29
0.54ArgCys: 0.54 ± 0.107
2.702ArgAsp: 2.702 ± 0.173
2.981ArgGlu: 2.981 ± 0.255
1.975ArgPhe: 1.975 ± 0.194
2.497ArgGly: 2.497 ± 0.216
0.913ArgHis: 0.913 ± 0.148
3.205ArgIle: 3.205 ± 0.251
3.112ArgLys: 3.112 ± 0.229
3.261ArgLeu: 3.261 ± 0.236
1.304ArgMet: 1.304 ± 0.144
2.012ArgAsn: 2.012 ± 0.185
1.342ArgPro: 1.342 ± 0.132
1.882ArgGln: 1.882 ± 0.219
2.124ArgArg: 2.124 ± 0.189
2.18ArgSer: 2.18 ± 0.199
2.422ArgThr: 2.422 ± 0.199
2.832ArgVal: 2.832 ± 0.248
0.615ArgTrp: 0.615 ± 0.128
1.677ArgTyr: 1.677 ± 0.182
0.0ArgXaa: 0.0 ± 0.0
Ser
3.634SerAla: 3.634 ± 0.256
0.876SerCys: 0.876 ± 0.137
3.447SerAsp: 3.447 ± 0.224
4.174SerGlu: 4.174 ± 0.296
2.646SerPhe: 2.646 ± 0.262
4.193SerGly: 4.193 ± 0.263
1.155SerHis: 1.155 ± 0.141
4.118SerIle: 4.118 ± 0.284
4.211SerLys: 4.211 ± 0.275
4.752SerLeu: 4.752 ± 0.304
1.621SerMet: 1.621 ± 0.171
2.926SerAsn: 2.926 ± 0.274
2.236SerPro: 2.236 ± 0.229
2.292SerGln: 2.292 ± 0.179
2.851SerArg: 2.851 ± 0.205
3.95SerSer: 3.95 ± 0.341
3.168SerThr: 3.168 ± 0.283
3.466SerVal: 3.466 ± 0.246
0.652SerTrp: 0.652 ± 0.114
2.683SerTyr: 2.683 ± 0.211
0.0SerXaa: 0.0 ± 0.0
Thr
4.36ThrAla: 4.36 ± 0.312
0.54ThrCys: 0.54 ± 0.101
3.242ThrAsp: 3.242 ± 0.284
4.081ThrGlu: 4.081 ± 0.297
2.907ThrPhe: 2.907 ± 0.25
4.658ThrGly: 4.658 ± 0.445
1.062ThrHis: 1.062 ± 0.169
3.913ThrIle: 3.913 ± 0.29
4.565ThrLys: 4.565 ± 0.246
4.77ThrLeu: 4.77 ± 0.316
1.267ThrMet: 1.267 ± 0.148
3.093ThrAsn: 3.093 ± 0.248
2.683ThrPro: 2.683 ± 0.242
2.106ThrGln: 2.106 ± 0.251
2.516ThrArg: 2.516 ± 0.256
3.671ThrSer: 3.671 ± 0.286
3.634ThrThr: 3.634 ± 0.417
4.23ThrVal: 4.23 ± 0.31
0.932ThrTrp: 0.932 ± 0.153
2.18ThrTyr: 2.18 ± 0.2
0.0ThrXaa: 0.0 ± 0.0
Val
4.435ValAla: 4.435 ± 0.316
1.081ValCys: 1.081 ± 0.156
4.472ValAsp: 4.472 ± 0.301
4.696ValGlu: 4.696 ± 0.365
3.0ValPhe: 3.0 ± 0.231
3.335ValGly: 3.335 ± 0.258
1.23ValHis: 1.23 ± 0.172
4.211ValIle: 4.211 ± 0.268
5.571ValLys: 5.571 ± 0.323
4.733ValLeu: 4.733 ± 0.254
1.882ValMet: 1.882 ± 0.202
3.652ValAsn: 3.652 ± 0.187
2.646ValPro: 2.646 ± 0.235
2.758ValGln: 2.758 ± 0.219
2.721ValArg: 2.721 ± 0.214
4.118ValSer: 4.118 ± 0.238
3.745ValThr: 3.745 ± 0.251
4.062ValVal: 4.062 ± 0.323
0.913ValTrp: 0.913 ± 0.13
2.702ValTyr: 2.702 ± 0.2
0.0ValXaa: 0.0 ± 0.0
Trp
1.099TrpAla: 1.099 ± 0.145
0.13TrpCys: 0.13 ± 0.045
0.894TrpAsp: 0.894 ± 0.127
1.006TrpGlu: 1.006 ± 0.151
0.671TrpPhe: 0.671 ± 0.108
0.391TrpGly: 0.391 ± 0.098
0.335TrpHis: 0.335 ± 0.086
0.801TrpIle: 0.801 ± 0.125
1.342TrpLys: 1.342 ± 0.139
1.211TrpLeu: 1.211 ± 0.164
0.41TrpMet: 0.41 ± 0.08
0.783TrpAsn: 0.783 ± 0.104
0.429TrpPro: 0.429 ± 0.089
0.522TrpGln: 0.522 ± 0.111
0.447TrpArg: 0.447 ± 0.1
0.876TrpSer: 0.876 ± 0.123
0.894TrpThr: 0.894 ± 0.134
1.099TrpVal: 1.099 ± 0.14
0.298TrpTrp: 0.298 ± 0.082
0.801TrpTyr: 0.801 ± 0.112
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.019TyrAla: 3.019 ± 0.231
0.466TyrCys: 0.466 ± 0.081
2.758TyrAsp: 2.758 ± 0.207
2.721TyrGlu: 2.721 ± 0.263
1.863TyrPhe: 1.863 ± 0.18
2.758TyrGly: 2.758 ± 0.236
0.913TyrHis: 0.913 ± 0.12
3.112TyrIle: 3.112 ± 0.24
3.13TyrLys: 3.13 ± 0.248
2.739TyrLeu: 2.739 ± 0.256
1.137TyrMet: 1.137 ± 0.163
2.683TyrAsn: 2.683 ± 0.223
1.547TyrPro: 1.547 ± 0.156
1.677TyrGln: 1.677 ± 0.183
1.64TyrArg: 1.64 ± 0.173
2.516TyrSer: 2.516 ± 0.24
2.385TyrThr: 2.385 ± 0.216
2.609TyrVal: 2.609 ± 0.216
0.708TyrTrp: 0.708 ± 0.12
1.882TyrTyr: 1.882 ± 0.18
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 293 proteins (53667 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski