Roseivivax isoporae LMG 25204
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4626 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|X7F3A6|X7F3A6_9RHOB Cation:proton antiporter OS=Roseivivax isoporae LMG 25204 OX=1449351 GN=RISW2_13910 PE=3 SV=1
MM1 pKa = 7.24 NRR3 pKa = 11.84 TAAIALAAALAAVPAVAQQSGEE25 pKa = 4.27 SLSGSLVLYY34 pKa = 8.97 TSQPDD39 pKa = 2.99 QDD41 pKa = 3.7 AQQTVDD47 pKa = 4.3 AFNEE51 pKa = 4.34 VYY53 pKa = 10.52 PDD55 pKa = 3.96 IDD57 pKa = 3.98 VEE59 pKa = 4.3 WVRR62 pKa = 11.84 DD63 pKa = 3.58 GTTQMMARR71 pKa = 11.84 LRR73 pKa = 11.84 AEE75 pKa = 4.42 FEE77 pKa = 4.19 AGQPQPDD84 pKa = 3.71 VLLIADD90 pKa = 4.56 TVTMEE95 pKa = 4.35 SLEE98 pKa = 4.12 QEE100 pKa = 4.58 GRR102 pKa = 11.84 LMAYY106 pKa = 9.06 TDD108 pKa = 4.44 ADD110 pKa = 3.43 VSAYY114 pKa = 10.41 DD115 pKa = 4.0 EE116 pKa = 4.62 GLMDD120 pKa = 3.89 PEE122 pKa = 5.21 GYY124 pKa = 10.53 YY125 pKa = 10.8 FSTKK129 pKa = 10.53 LITTGIVYY137 pKa = 10.59 NEE139 pKa = 4.15 AAEE142 pKa = 4.27 MVPQSYY148 pKa = 11.59 ADD150 pKa = 3.91 LLEE153 pKa = 4.38 PAAEE157 pKa = 4.21 NNIVMPSPLEE167 pKa = 4.14 SGAATIHH174 pKa = 5.55 MVSLTGLPEE183 pKa = 4.64 LGWDD187 pKa = 4.07 YY188 pKa = 11.54 YY189 pKa = 11.25 QGLADD194 pKa = 4.21 QGAIAQGGNGGTYY207 pKa = 9.3 QAVAGGQALYY217 pKa = 10.97 GFVVDD222 pKa = 7.14 FLALRR227 pKa = 11.84 NIQDD231 pKa = 3.68 GAPVGFVFPEE241 pKa = 4.09 EE242 pKa = 4.17 GVSAVTEE249 pKa = 3.97 PVAILEE255 pKa = 4.23 TARR258 pKa = 11.84 NVEE261 pKa = 3.92 AAQAFVDD268 pKa = 4.39 FLLSEE273 pKa = 4.89 RR274 pKa = 11.84 GQEE277 pKa = 3.61 LAAAQGYY284 pKa = 9.47 LPAHH288 pKa = 7.25 PDD290 pKa = 3.08 VAAPDD295 pKa = 3.95 YY296 pKa = 10.93 FPDD299 pKa = 3.7 RR300 pKa = 11.84 STIKK304 pKa = 10.94 VIDD307 pKa = 4.02 FDD309 pKa = 4.24 PAEE312 pKa = 4.31 ALANDD317 pKa = 3.75 AAYY320 pKa = 9.76 KK321 pKa = 10.42 EE322 pKa = 4.48 RR323 pKa = 11.84 FAEE326 pKa = 4.32 TFGGG330 pKa = 3.75
Molecular weight: 35.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.757
IPC_protein 3.757
Toseland 3.554
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.643
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.037
Thurlkill 3.592
EMBOSS 3.656
Sillero 3.872
Patrickios 1.176
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.769
Protein with the highest isoelectric point:
>tr|X7F8R9|X7F8R9_9RHOB Uncharacterized protein OS=Roseivivax isoporae LMG 25204 OX=1449351 GN=RISW2_06215 PE=4 SV=1
MM1 pKa = 7.5 SIKK4 pKa = 10.86 GIVTKK9 pKa = 9.85 VAKK12 pKa = 10.32 GYY14 pKa = 10.35 ARR16 pKa = 11.84 SKK18 pKa = 9.8 SGHH21 pKa = 5.07 GAARR25 pKa = 11.84 RR26 pKa = 11.84 PVNRR30 pKa = 11.84 PVRR33 pKa = 11.84 RR34 pKa = 11.84 TGGKK38 pKa = 8.26 GQLVRR43 pKa = 11.84 GLLNFARR50 pKa = 11.84 KK51 pKa = 9.19 KK52 pKa = 9.12 VV53 pKa = 3.34
Molecular weight: 5.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.364
IPC2_protein 11.008
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.31
Grimsley 12.544
Solomon 12.983
Lehninger 12.881
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.983
Sillero 12.501
Patrickios 12.047
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.004
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4626
0
4626
1446104
28
5196
312.6
33.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.849 ± 0.063
0.829 ± 0.012
6.347 ± 0.036
5.828 ± 0.034
3.512 ± 0.023
9.253 ± 0.045
1.996 ± 0.019
4.466 ± 0.028
2.037 ± 0.026
10.187 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.548 ± 0.017
2.082 ± 0.021
5.369 ± 0.024
2.693 ± 0.019
7.862 ± 0.043
4.619 ± 0.024
5.437 ± 0.026
7.653 ± 0.03
1.403 ± 0.015
2.029 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here