Legionella fallonii LLAP-10
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3840 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A098G471|A0A098G471_9GAMM Methionine aminopeptidase OS=Legionella fallonii LLAP-10 OX=1212491 GN=map PE=3 SV=1
MM1 pKa = 7.42 AAIDD5 pKa = 3.99 VSPATTDD12 pKa = 2.79 ITFSISAADD21 pKa = 3.97 KK22 pKa = 9.56 VAEE25 pKa = 4.75 LIKK28 pKa = 11.1 EE29 pKa = 3.86 EE30 pKa = 5.53 DD31 pKa = 3.63 NFNLNLRR38 pKa = 11.84 VFITGGGCSGFQYY51 pKa = 10.68 GFSFDD56 pKa = 3.7 EE57 pKa = 4.42 QVNEE61 pKa = 4.21 DD62 pKa = 3.77 DD63 pKa = 4.11 TVVEE67 pKa = 4.25 QHH69 pKa = 6.72 CSDD72 pKa = 3.33 GDD74 pKa = 3.95 TTVKK78 pKa = 10.8 LLVDD82 pKa = 3.8 SMSYY86 pKa = 10.53 QYY88 pKa = 11.57 LHH90 pKa = 7.17 DD91 pKa = 4.61 AEE93 pKa = 4.55 IDD95 pKa = 3.89 YY96 pKa = 10.99 VQGIQGEE103 pKa = 4.31 QFIIRR108 pKa = 11.84 NPNAKK113 pKa = 6.56 TTCGCGSSFSMDD125 pKa = 4.89 DD126 pKa = 3.7 EE127 pKa = 6.97 DD128 pKa = 6.12 DD129 pKa = 3.7 LL130 pKa = 6.64
Molecular weight: 14.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.732
IPC_protein 3.719
Toseland 3.503
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.668
Rodwell 3.541
Grimsley 3.414
Solomon 3.706
Lehninger 3.656
Nozaki 3.834
DTASelect 4.075
Thurlkill 3.567
EMBOSS 3.681
Sillero 3.846
Patrickios 0.947
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.747
Protein with the highest isoelectric point:
>tr|A0A098G1Y8|A0A098G1Y8_9GAMM Protein PmbA OS=Legionella fallonii LLAP-10 OX=1212491 GN=pmbA PE=3 SV=1
MM1 pKa = 7.84 RR2 pKa = 11.84 NNAPKK7 pKa = 9.78 IGARR11 pKa = 11.84 IGSRR15 pKa = 11.84 GVAAVAVAARR25 pKa = 11.84 AKK27 pKa = 10.35 AAWAVKK33 pKa = 9.98 AVAAALLAIAAMVPAVSRR51 pKa = 11.84 AAAVNAPKK59 pKa = 10.47 HH60 pKa = 5.5 PNIKK64 pKa = 10.3
Molecular weight: 6.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.408
IPC2_protein 10.891
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.457
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.193
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.023
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3840
0
3840
1280907
22
4859
333.6
37.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.582 ± 0.04
1.166 ± 0.017
4.871 ± 0.031
6.023 ± 0.05
4.38 ± 0.031
5.882 ± 0.039
2.552 ± 0.018
7.48 ± 0.036
6.216 ± 0.042
10.915 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.374 ± 0.022
4.982 ± 0.03
4.057 ± 0.028
4.775 ± 0.043
4.128 ± 0.027
6.737 ± 0.033
5.398 ± 0.026
5.901 ± 0.032
1.11 ± 0.017
3.47 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here