Wuhan house centipede virus 6
Average proteome isoelectric point is 8.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KGS6|A0A1L3KGS6_9VIRU Uncharacterized protein OS=Wuhan house centipede virus 6 OX=1923710 PE=4 SV=1
MM1 pKa = 7.29 SQMLFNTCDD10 pKa = 3.45 VVKK13 pKa = 10.08 STFLMMRR20 pKa = 11.84 TYY22 pKa = 10.53 PKK24 pKa = 9.34 TVLAITVTSYY34 pKa = 11.97 GLVKK38 pKa = 10.56 LIKK41 pKa = 10.16 HH42 pKa = 5.59 HH43 pKa = 6.88 AKK45 pKa = 10.43 LYY47 pKa = 8.12 NQQNPFEE54 pKa = 4.68 YY55 pKa = 10.14 IPSEE59 pKa = 3.99 EE60 pKa = 4.43 TNWLLVRR67 pKa = 11.84 QVRR70 pKa = 11.84 NVLKK74 pKa = 10.2 HH75 pKa = 5.04 QYY77 pKa = 10.7 AAVKK81 pKa = 8.49 YY82 pKa = 10.61 QMMTVGGLYY91 pKa = 7.79 QTNVKK96 pKa = 9.81 QLSEE100 pKa = 4.58 EE101 pKa = 3.93 IEE103 pKa = 3.89 KK104 pKa = 10.16 TIIRR108 pKa = 11.84 KK109 pKa = 9.13 PDD111 pKa = 3.41 LRR113 pKa = 11.84 PTLVPNGDD121 pKa = 4.36 SIDD124 pKa = 4.21 IIPVNSMHH132 pKa = 6.25 SHH134 pKa = 6.55 PASAQFRR141 pKa = 11.84 SSANSYY147 pKa = 11.33 LNDD150 pKa = 3.53 CVVRR154 pKa = 11.84 AGYY157 pKa = 9.99 RR158 pKa = 11.84 PYY160 pKa = 10.68 NISKK164 pKa = 9.92 SRR166 pKa = 11.84 RR167 pKa = 11.84 DD168 pKa = 3.3 IEE170 pKa = 4.35 DD171 pKa = 3.32 GCRR174 pKa = 11.84 YY175 pKa = 9.88 FYY177 pKa = 10.64 HH178 pKa = 7.18 SKK180 pKa = 10.69 DD181 pKa = 3.61 LSIKK185 pKa = 9.95 YY186 pKa = 10.62 SNDD189 pKa = 3.28 KK190 pKa = 11.0 IEE192 pKa = 4.55 DD193 pKa = 3.33 QSVFIMTDD201 pKa = 2.15 VDD203 pKa = 4.4 YY204 pKa = 11.26 YY205 pKa = 11.63 VDD207 pKa = 3.46 MPRR210 pKa = 11.84 WLKK213 pKa = 10.38 LFRR216 pKa = 11.84 PIVMYY221 pKa = 9.65 TLSPTKK227 pKa = 10.45 LSCSGPNDD235 pKa = 3.3 KK236 pKa = 11.17 AEE238 pKa = 3.73 YY239 pKa = 10.16 RR240 pKa = 11.84 FNIDD244 pKa = 3.05 GNVVHH249 pKa = 5.97 YY250 pKa = 9.81 HH251 pKa = 4.66 VSGGGEE257 pKa = 3.98 YY258 pKa = 8.71 THH260 pKa = 6.48 QLWNYY265 pKa = 8.41 TGDD268 pKa = 3.41 TVTCVDD274 pKa = 4.64 DD275 pKa = 5.6 DD276 pKa = 4.96 GNLLTFDD283 pKa = 3.4 IEE285 pKa = 4.17 QRR287 pKa = 11.84 KK288 pKa = 9.09 VKK290 pKa = 10.46 GDD292 pKa = 3.48 EE293 pKa = 3.68 QHH295 pKa = 6.71 RR296 pKa = 11.84 LIWLLPRR303 pKa = 11.84 SKK305 pKa = 9.45 VTNPLWHH312 pKa = 6.53 YY313 pKa = 11.1 LRR315 pKa = 11.84 LDD317 pKa = 3.41 WEE319 pKa = 4.45 NNLLKK324 pKa = 10.69 RR325 pKa = 11.84 KK326 pKa = 10.35 DD327 pKa = 3.44 MTLKK331 pKa = 10.5 GFNYY335 pKa = 10.05 LLEE338 pKa = 5.11 PIEE341 pKa = 5.06 DD342 pKa = 3.77 NLSIGLIGTKK352 pKa = 10.29 YY353 pKa = 10.44 SVEE356 pKa = 4.14 IPGKK360 pKa = 9.29 LYY362 pKa = 9.55 EE363 pKa = 4.73 AIRR366 pKa = 11.84 IRR368 pKa = 11.84 LAEE371 pKa = 4.08 KK372 pKa = 9.39 EE373 pKa = 4.13 ATVFVSDD380 pKa = 3.63 VEE382 pKa = 4.6 RR383 pKa = 11.84 MLKK386 pKa = 9.04 EE387 pKa = 4.35 AKK389 pKa = 9.19 HH390 pKa = 4.97 QSYY393 pKa = 7.97 VTDD396 pKa = 3.7 APILFKK402 pKa = 10.99 CFAVDD407 pKa = 3.89 VVFDD411 pKa = 4.15 KK412 pKa = 11.55 NVVKK416 pKa = 10.09 TSNFAVTYY424 pKa = 9.0 QAIPRR429 pKa = 11.84 HH430 pKa = 5.69 GALSTEE436 pKa = 4.67 DD437 pKa = 3.87 GNNPGQTTTSPLTSNPALFASKK459 pKa = 10.56 GYY461 pKa = 9.96 NADD464 pKa = 3.52 KK465 pKa = 11.05 ACIEE469 pKa = 4.0 GRR471 pKa = 11.84 IHH473 pKa = 7.31 KK474 pKa = 9.31 VANNKK479 pKa = 8.84 KK480 pKa = 8.52 WSKK483 pKa = 10.36 KK484 pKa = 6.7 YY485 pKa = 10.1 HH486 pKa = 6.39 DD487 pKa = 4.1 YY488 pKa = 11.25 ARR490 pKa = 11.84 EE491 pKa = 4.21 FIDD494 pKa = 3.57 RR495 pKa = 11.84 LVPKK499 pKa = 9.76 RR500 pKa = 11.84 YY501 pKa = 9.68 RR502 pKa = 11.84 GTGTPLSIGEE512 pKa = 3.83 VDD514 pKa = 3.64 LRR516 pKa = 11.84 QEE518 pKa = 3.99 KK519 pKa = 9.2 VAQRR523 pKa = 11.84 GRR525 pKa = 11.84 FKK527 pKa = 10.7 QVAPMMSLDD536 pKa = 3.6 AGNAIKK542 pKa = 10.75 AFIKK546 pKa = 10.04 TEE548 pKa = 4.23 TYY550 pKa = 11.13 ASAKK554 pKa = 8.85 PPRR557 pKa = 11.84 NISTMSPEE565 pKa = 3.97 LTIQMSAYY573 pKa = 8.67 TLVFANILKK582 pKa = 8.5 THH584 pKa = 6.9 DD585 pKa = 3.56 WYY587 pKa = 11.54 CPGKK591 pKa = 10.22 KK592 pKa = 9.59 PRR594 pKa = 11.84 DD595 pKa = 3.49 IIKK598 pKa = 10.53 RR599 pKa = 11.84 LAHH602 pKa = 5.07 VMKK605 pKa = 10.73 AEE607 pKa = 3.94 EE608 pKa = 4.42 TEE610 pKa = 4.08 DD611 pKa = 3.96 VEE613 pKa = 4.9 EE614 pKa = 5.2 GDD616 pKa = 4.15 YY617 pKa = 11.08 TCLDD621 pKa = 3.59 GTQSEE626 pKa = 5.13 EE627 pKa = 4.01 YY628 pKa = 10.56 ARR630 pKa = 11.84 NLLLPAYY637 pKa = 7.78 MQYY640 pKa = 10.98 LAPEE644 pKa = 4.34 HH645 pKa = 6.0 RR646 pKa = 11.84 TMFKK650 pKa = 10.78 DD651 pKa = 4.22 LYY653 pKa = 10.04 KK654 pKa = 10.46 KK655 pKa = 10.45 VYY657 pKa = 8.61 KK658 pKa = 10.29 QHH660 pKa = 6.28 ATTSTGVSYY669 pKa = 11.1 DD670 pKa = 2.81 PGYY673 pKa = 8.72 TVRR676 pKa = 11.84 SGSPITTQAGTLANAFNVYY695 pKa = 10.06 SALRR699 pKa = 11.84 NMGYY703 pKa = 10.15 VPDD706 pKa = 4.46 EE707 pKa = 4.36 AFDD710 pKa = 3.84 MIGAIFGDD718 pKa = 4.47 DD719 pKa = 3.57 SCNPNYY725 pKa = 10.15 KK726 pKa = 10.39 GQFHH730 pKa = 7.0 LFIEE734 pKa = 4.74 QVAKK738 pKa = 10.41 DD739 pKa = 3.55 LGMIYY744 pKa = 10.34 KK745 pKa = 10.57 SNLRR749 pKa = 11.84 PRR751 pKa = 11.84 GEE753 pKa = 3.9 PLLFLGRR760 pKa = 11.84 YY761 pKa = 8.42 FVDD764 pKa = 4.86 PPTTDD769 pKa = 4.07 DD770 pKa = 4.5 SYY772 pKa = 12.1 ADD774 pKa = 3.72 PLRR777 pKa = 11.84 TIGKK781 pKa = 8.69 LHH783 pKa = 6.91 ASTNKK788 pKa = 10.22 SVTKK792 pKa = 10.09 EE793 pKa = 3.26 QAAANKK799 pKa = 9.98 ALGYY803 pKa = 10.55 LSTDD807 pKa = 2.8 RR808 pKa = 11.84 LTPIIGTWAARR819 pKa = 11.84 VIQLTKK825 pKa = 9.98 IRR827 pKa = 11.84 TMKK830 pKa = 10.64 GGTGEE835 pKa = 4.06 EE836 pKa = 4.31 QYY838 pKa = 10.79 KK839 pKa = 10.71 CSNAWPQKK847 pKa = 9.55 DD848 pKa = 3.27 EE849 pKa = 4.05 TRR851 pKa = 11.84 IRR853 pKa = 11.84 EE854 pKa = 4.26 CMATTLGITTAEE866 pKa = 4.74 LIEE869 pKa = 4.53 LDD871 pKa = 3.52 KK872 pKa = 10.96 KK873 pKa = 10.5 IRR875 pKa = 11.84 SVDD878 pKa = 3.65 GLDD881 pKa = 3.45 HH882 pKa = 6.71 FPVIFDD888 pKa = 3.39 TAYY891 pKa = 9.9 KK892 pKa = 7.8 HH893 pKa = 5.02 TQVAVVDD900 pKa = 4.11 GDD902 pKa = 4.16 LVCTDD907 pKa = 3.42 LHH909 pKa = 7.72 QDD911 pKa = 3.21 IEE913 pKa = 4.78 PIHH916 pKa = 7.06 DD917 pKa = 3.59 EE918 pKa = 4.42 SEE920 pKa = 4.3 PKK922 pKa = 10.43 ASCSGIQSPSSSHH935 pKa = 6.3 RR936 pKa = 11.84 SCSAKK941 pKa = 9.48 ATEE944 pKa = 4.16 RR945 pKa = 11.84 PKK947 pKa = 10.76 PGFTEE952 pKa = 4.84 RR953 pKa = 11.84 SFKK956 pKa = 10.3 TSGRR960 pKa = 11.84 RR961 pKa = 11.84 ATGKK965 pKa = 9.54 GHH967 pKa = 7.1 PNRR970 pKa = 11.84 IRR972 pKa = 11.84 PNTRR976 pKa = 11.84 KK977 pKa = 10.04 SNRR980 pKa = 11.84 VDD982 pKa = 3.26 PRR984 pKa = 11.84 DD985 pKa = 3.75 DD986 pKa = 3.44 VPRR989 pKa = 11.84 TDD991 pKa = 3.32 GRR993 pKa = 11.84 RR994 pKa = 11.84 INN996 pKa = 3.54
Molecular weight: 113.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.129
IPC2_protein 8.214
IPC_protein 8.053
Toseland 8.375
ProMoST 8.595
Dawson 8.931
Bjellqvist 8.946
Wikipedia 9.033
Rodwell 9.033
Grimsley 8.712
Solomon 8.96
Lehninger 8.931
Nozaki 9.019
DTASelect 8.799
Thurlkill 8.873
EMBOSS 9.063
Sillero 9.092
Patrickios 4.368
IPC_peptide 8.96
IPC2_peptide 7.658
IPC2.peptide.svr19 7.719
Protein with the highest isoelectric point:
>tr|A0A1L3KGY7|A0A1L3KGY7_9VIRU Capsid protein OS=Wuhan house centipede virus 6 OX=1923710 PE=3 SV=1
MM1 pKa = 7.38 VNKK4 pKa = 10.16 NNQKK8 pKa = 10.18 ARR10 pKa = 11.84 NGKK13 pKa = 6.48 TKK15 pKa = 10.49 RR16 pKa = 11.84 KK17 pKa = 7.1 TRR19 pKa = 11.84 NGQLQATRR27 pKa = 11.84 EE28 pKa = 4.5 GPASSRR34 pKa = 11.84 SGMRR38 pKa = 11.84 TGIPRR43 pKa = 11.84 IRR45 pKa = 11.84 NRR47 pKa = 11.84 DD48 pKa = 3.36 PYY50 pKa = 10.98 SIEE53 pKa = 3.86 VCNTEE58 pKa = 3.2 IAMTITGTAAGGIIPAGGNIKK79 pKa = 9.9 IMRR82 pKa = 11.84 FDD84 pKa = 4.57 DD85 pKa = 4.04 SSTGNALNNKK95 pKa = 8.67 HH96 pKa = 6.63 WITKK100 pKa = 10.21 LGLAYY105 pKa = 10.31 DD106 pKa = 3.63 KK107 pKa = 10.98 FVIEE111 pKa = 4.23 EE112 pKa = 4.25 LSMKK116 pKa = 9.68 FVPSLPFTAAGMSAMYY132 pKa = 10.15 FDD134 pKa = 5.44 SDD136 pKa = 4.01 PSRR139 pKa = 11.84 TTPPTSVASVSGDD152 pKa = 2.9 MRR154 pKa = 11.84 AVSKK158 pKa = 10.47 QIYY161 pKa = 10.22 AEE163 pKa = 4.41 LPLKK167 pKa = 10.18 VLRR170 pKa = 11.84 NQLNRR175 pKa = 11.84 LPQYY179 pKa = 7.97 EE180 pKa = 4.42 TFPGSGDD187 pKa = 3.31 TGVATVGSINFVHH200 pKa = 7.51 DD201 pKa = 4.93 SIAMPNASTTGNITIGNVWMTYY223 pKa = 9.99 RR224 pKa = 11.84 IRR226 pKa = 11.84 LINPSNAVAA235 pKa = 4.28
Molecular weight: 25.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.215
IPC2_protein 9.472
IPC_protein 9.677
Toseland 10.277
ProMoST 9.911
Dawson 10.423
Bjellqvist 10.087
Wikipedia 10.584
Rodwell 10.833
Grimsley 10.482
Solomon 10.467
Lehninger 10.438
Nozaki 10.248
DTASelect 10.087
Thurlkill 10.292
EMBOSS 10.657
Sillero 10.335
Patrickios 10.526
IPC_peptide 10.467
IPC2_peptide 8.712
IPC2.peptide.svr19 8.607
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1387
156
996
462.3
51.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.705 ± 0.677
1.154 ± 0.467
5.84 ± 0.944
4.398 ± 0.959
2.812 ± 0.702
6.273 ± 0.875
2.235 ± 0.653
5.984 ± 0.614
6.2 ± 1.607
7.282 ± 0.797
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.596 ± 0.623
5.696 ± 1.303
5.552 ± 0.308
3.317 ± 0.348
6.921 ± 1.333
6.921 ± 0.625
8.435 ± 1.36
6.273 ± 0.385
0.937 ± 0.092
4.47 ± 0.805
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here