Amino acid dipepetide frequency for Lachnospiraceae bacterium OM04-12BH

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.879AlaAla: 7.879 ± 0.13	1.168AlaCys: 1.168 ± 0.041	4.698AlaAsp: 4.698 ± 0.081	6.101AlaGlu: 6.101 ± 0.089	3.196AlaPhe: 3.196 ± 0.061	6.662AlaGly: 6.662 ± 0.092	1.064AlaHis: 1.064 ± 0.034	4.782AlaIle: 4.782 ± 0.077	4.12AlaLys: 4.12 ± 0.074	7.013AlaLeu: 7.013 ± 0.082	2.42AlaMet: 2.42 ± 0.051	2.26AlaAsn: 2.26 ± 0.045	2.036AlaPro: 2.036 ± 0.054	2.223AlaGln: 2.223 ± 0.06	3.202AlaArg: 3.202 ± 0.063	4.0AlaSer: 4.0 ± 0.076	3.053AlaThr: 3.053 ± 0.067	6.524AlaVal: 6.524 ± 0.099	0.553AlaTrp: 0.553 ± 0.025	2.961AlaTyr: 2.961 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	1.0CysAla: 1.0 ± 0.035	0.332CysCys: 0.332 ± 0.02	0.817CysAsp: 0.817 ± 0.031	0.94CysGlu: 0.94 ± 0.032	0.73CysPhe: 0.73 ± 0.028	1.494CysGly: 1.494 ± 0.039	0.313CysHis: 0.313 ± 0.018	1.088CysIle: 1.088 ± 0.036	0.773CysLys: 0.773 ± 0.027	1.252CysLeu: 1.252 ± 0.038	0.531CysMet: 0.531 ± 0.025	0.604CysAsn: 0.604 ± 0.024	0.575CysPro: 0.575 ± 0.025	0.46CysGln: 0.46 ± 0.022	0.862CysArg: 0.862 ± 0.031	0.865CysSer: 0.865 ± 0.034	0.646CysThr: 0.646 ± 0.025	1.036CysVal: 1.036 ± 0.036	0.125CysTrp: 0.125 ± 0.011	0.638CysTyr: 0.638 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	3.983AspAla: 3.983 ± 0.07	0.757AspCys: 0.757 ± 0.034	2.712AspAsp: 2.712 ± 0.066	4.5AspGlu: 4.5 ± 0.082	2.605AspPhe: 2.605 ± 0.059	4.293AspGly: 4.293 ± 0.072	0.909AspHis: 0.909 ± 0.032	4.337AspIle: 4.337 ± 0.071	3.362AspLys: 3.362 ± 0.06	4.398AspLeu: 4.398 ± 0.072	1.889AspMet: 1.889 ± 0.047	2.078AspAsn: 2.078 ± 0.045	1.918AspPro: 1.918 ± 0.049	1.47AspGln: 1.47 ± 0.041	2.793AspArg: 2.793 ± 0.063	3.062AspSer: 3.062 ± 0.06	3.102AspThr: 3.102 ± 0.06	3.493AspVal: 3.493 ± 0.071	0.539AspTrp: 0.539 ± 0.026	2.955AspTyr: 2.955 ± 0.058	0.0AspXaa: 0.0 ± 0.0
Glu	6.124GluAla: 6.124 ± 0.094	0.825GluCys: 0.825 ± 0.032	4.496GluAsp: 4.496 ± 0.075	8.377GluGlu: 8.377 ± 0.143	2.451GluPhe: 2.451 ± 0.055	4.976GluGly: 4.976 ± 0.076	1.245GluHis: 1.245 ± 0.04	5.715GluIle: 5.715 ± 0.089	7.433GluLys: 7.433 ± 0.096	7.013GluLeu: 7.013 ± 0.089	2.496GluMet: 2.496 ± 0.05	4.248GluAsn: 4.248 ± 0.063	1.818GluPro: 1.818 ± 0.045	2.984GluGln: 2.984 ± 0.062	3.719GluArg: 3.719 ± 0.065	3.399GluSer: 3.399 ± 0.06	3.95GluThr: 3.95 ± 0.07	4.428GluVal: 4.428 ± 0.062	0.689GluTrp: 0.689 ± 0.027	3.047GluTyr: 3.047 ± 0.059	0.0GluXaa: 0.0 ± 0.0
Phe	3.115PheAla: 3.115 ± 0.064	0.827PheCys: 0.827 ± 0.034	2.463PheAsp: 2.463 ± 0.051	2.592PheGlu: 2.592 ± 0.051	2.011PhePhe: 2.011 ± 0.058	3.061PheGly: 3.061 ± 0.057	0.89PheHis: 0.89 ± 0.032	2.583PheIle: 2.583 ± 0.059	1.926PheLys: 1.926 ± 0.043	4.525PheLeu: 4.525 ± 0.096	1.253PheMet: 1.253 ± 0.039	1.408PheAsn: 1.408 ± 0.041	1.445PhePro: 1.445 ± 0.034	1.335PheGln: 1.335 ± 0.034	1.792PheArg: 1.792 ± 0.046	2.876PheSer: 2.876 ± 0.056	2.413PheThr: 2.413 ± 0.053	2.767PheVal: 2.767 ± 0.053	0.394PheTrp: 0.394 ± 0.019	1.796PheTyr: 1.796 ± 0.049	0.0PheXaa: 0.0 ± 0.0
Gly	4.859GlyAla: 4.859 ± 0.087	1.283GlyCys: 1.283 ± 0.043	3.396GlyAsp: 3.396 ± 0.06	5.065GlyGlu: 5.065 ± 0.086	3.107GlyPhe: 3.107 ± 0.057	4.603GlyGly: 4.603 ± 0.095	1.197GlyHis: 1.197 ± 0.039	6.55GlyIle: 6.55 ± 0.087	5.344GlyLys: 5.344 ± 0.075	5.747GlyLeu: 5.747 ± 0.085	2.7GlyMet: 2.7 ± 0.06	3.219GlyAsn: 3.219 ± 0.055	1.146GlyPro: 1.146 ± 0.035	2.062GlyGln: 2.062 ± 0.046	3.528GlyArg: 3.528 ± 0.06	4.232GlySer: 4.232 ± 0.072	4.1GlyThr: 4.1 ± 0.067	4.557GlyVal: 4.557 ± 0.076	0.655GlyTrp: 0.655 ± 0.031	3.396GlyTyr: 3.396 ± 0.069	0.0GlyXaa: 0.0 ± 0.0
His	1.062HisAla: 1.062 ± 0.031	0.265HisCys: 0.265 ± 0.016	0.843HisAsp: 0.843 ± 0.035	1.047HisGlu: 1.047 ± 0.036	0.854HisPhe: 0.854 ± 0.031	1.214HisGly: 1.214 ± 0.035	0.383HisHis: 0.383 ± 0.028	1.343HisIle: 1.343 ± 0.04	0.942HisLys: 0.942 ± 0.031	1.534HisLeu: 1.534 ± 0.045	0.566HisMet: 0.566 ± 0.025	0.709HisAsn: 0.709 ± 0.025	0.832HisPro: 0.832 ± 0.034	0.513HisGln: 0.513 ± 0.023	0.841HisArg: 0.841 ± 0.03	0.956HisSer: 0.956 ± 0.029	0.936HisThr: 0.936 ± 0.027	1.058HisVal: 1.058 ± 0.035	0.154HisTrp: 0.154 ± 0.011	0.803HisTyr: 0.803 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.663IleAla: 5.663 ± 0.071	1.418IleCys: 1.418 ± 0.044	3.87IleAsp: 3.87 ± 0.066	4.665IleGlu: 4.665 ± 0.075	2.869IlePhe: 2.869 ± 0.063	5.031IleGly: 5.031 ± 0.083	1.34IleHis: 1.34 ± 0.035	4.927IleIle: 4.927 ± 0.092	3.697IleLys: 3.697 ± 0.074	7.427IleLeu: 7.427 ± 0.109	2.049IleMet: 2.049 ± 0.051	2.784IleAsn: 2.784 ± 0.059	3.228IlePro: 3.228 ± 0.063	2.209IleGln: 2.209 ± 0.054	4.135IleArg: 4.135 ± 0.073	5.033IleSer: 5.033 ± 0.081	4.284IleThr: 4.284 ± 0.073	4.72IleVal: 4.72 ± 0.081	0.684IleTrp: 0.684 ± 0.028	2.963IleTyr: 2.963 ± 0.063	0.0IleXaa: 0.0 ± 0.0
Lys	4.936LysAla: 4.936 ± 0.088	0.637LysCys: 0.637 ± 0.027	3.754LysAsp: 3.754 ± 0.076	7.009LysGlu: 7.009 ± 0.09	1.707LysPhe: 1.707 ± 0.041	4.264LysGly: 4.264 ± 0.058	0.938LysHis: 0.938 ± 0.028	4.419LysIle: 4.419 ± 0.068	6.053LysLys: 6.053 ± 0.095	5.332LysLeu: 5.332 ± 0.076	2.009LysMet: 2.009 ± 0.048	3.37LysAsn: 3.37 ± 0.055	1.941LysPro: 1.941 ± 0.044	2.45LysGln: 2.45 ± 0.052	3.201LysArg: 3.201 ± 0.063	3.021LysSer: 3.021 ± 0.054	3.623LysThr: 3.623 ± 0.06	3.9LysVal: 3.9 ± 0.072	0.651LysTrp: 0.651 ± 0.027	2.677LysTyr: 2.677 ± 0.062	0.0LysXaa: 0.0 ± 0.0
Leu	6.977LeuAla: 6.977 ± 0.092	1.635LeuCys: 1.635 ± 0.046	4.68LeuAsp: 4.68 ± 0.074	5.709LeuGlu: 5.709 ± 0.086	4.305LeuPhe: 4.305 ± 0.083	5.371LeuGly: 5.371 ± 0.07	1.584LeuHis: 1.584 ± 0.04	6.594LeuIle: 6.594 ± 0.106	6.128LeuLys: 6.128 ± 0.074	9.798LeuLeu: 9.798 ± 0.14	2.755LeuMet: 2.755 ± 0.058	3.532LeuAsn: 3.532 ± 0.064	3.751LeuPro: 3.751 ± 0.071	3.506LeuGln: 3.506 ± 0.067	3.598LeuArg: 3.598 ± 0.068	6.871LeuSer: 6.871 ± 0.101	5.575LeuThr: 5.575 ± 0.088	5.591LeuVal: 5.591 ± 0.092	0.753LeuTrp: 0.753 ± 0.03	3.571LeuTyr: 3.571 ± 0.069	0.0LeuXaa: 0.0 ± 0.0
Met	2.532MetAla: 2.532 ± 0.047	0.374MetCys: 0.374 ± 0.02	1.876MetAsp: 1.876 ± 0.038	2.7MetGlu: 2.7 ± 0.06	0.945MetPhe: 0.945 ± 0.034	2.064MetGly: 2.064 ± 0.049	0.446MetHis: 0.446 ± 0.025	2.38MetIle: 2.38 ± 0.056	2.728MetLys: 2.728 ± 0.055	2.854MetLeu: 2.854 ± 0.056	0.976MetMet: 0.976 ± 0.034	1.569MetAsn: 1.569 ± 0.042	1.126MetPro: 1.126 ± 0.035	1.112MetGln: 1.112 ± 0.034	1.242MetArg: 1.242 ± 0.035	1.676MetSer: 1.676 ± 0.047	1.828MetThr: 1.828 ± 0.045	2.016MetVal: 2.016 ± 0.048	0.221MetTrp: 0.221 ± 0.017	0.906MetTyr: 0.906 ± 0.03	0.0MetXaa: 0.0 ± 0.0
Asn	3.061AsnAla: 3.061 ± 0.06	0.598AsnCys: 0.598 ± 0.025	2.058AsnAsp: 2.058 ± 0.049	2.757AsnGlu: 2.757 ± 0.057	1.475AsnPhe: 1.475 ± 0.041	3.392AsnGly: 3.392 ± 0.065	0.754AsnHis: 0.754 ± 0.026	3.28AsnIle: 3.28 ± 0.062	2.262AsnLys: 2.262 ± 0.05	3.486AsnLeu: 3.486 ± 0.061	1.362AsnMet: 1.362 ± 0.037	1.671AsnAsn: 1.671 ± 0.048	1.825AsnPro: 1.825 ± 0.049	1.461AsnGln: 1.461 ± 0.044	2.136AsnArg: 2.136 ± 0.048	2.228AsnSer: 2.228 ± 0.046	2.297AsnThr: 2.297 ± 0.058	2.7AsnVal: 2.7 ± 0.056	0.377AsnTrp: 0.377 ± 0.019	1.826AsnTyr: 1.826 ± 0.044	0.0AsnXaa: 0.0 ± 0.0
Pro	2.593ProAla: 2.593 ± 0.058	0.464ProCys: 0.464 ± 0.021	2.358ProAsp: 2.358 ± 0.053	3.318ProGlu: 3.318 ± 0.065	1.644ProPhe: 1.644 ± 0.043	2.339ProGly: 2.339 ± 0.05	0.54ProHis: 0.54 ± 0.021	1.906ProIle: 1.906 ± 0.046	1.629ProLys: 1.629 ± 0.047	2.762ProLeu: 2.762 ± 0.052	0.856ProMet: 0.856 ± 0.029	0.97ProAsn: 0.97 ± 0.033	0.753ProPro: 0.753 ± 0.029	0.921ProGln: 0.921 ± 0.032	0.958ProArg: 0.958 ± 0.036	1.86ProSer: 1.86 ± 0.045	1.416ProThr: 1.416 ± 0.037	3.245ProVal: 3.245 ± 0.059	0.31ProTrp: 0.31 ± 0.02	1.598ProTyr: 1.598 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	2.514GlnAla: 2.514 ± 0.061	0.328GlnCys: 0.328 ± 0.017	1.663GlnAsp: 1.663 ± 0.046	3.017GlnGlu: 3.017 ± 0.065	1.159GlnPhe: 1.159 ± 0.036	1.926GlnGly: 1.926 ± 0.051	0.459GlnHis: 0.459 ± 0.02	2.645GlnIle: 2.645 ± 0.057	2.943GlnLys: 2.943 ± 0.059	2.943GlnLeu: 2.943 ± 0.063	1.197GlnMet: 1.197 ± 0.037	1.606GlnAsn: 1.606 ± 0.041	0.869GlnPro: 0.869 ± 0.028	1.283GlnGln: 1.283 ± 0.044	1.454GlnArg: 1.454 ± 0.039	1.624GlnSer: 1.624 ± 0.039	1.741GlnThr: 1.741 ± 0.044	2.079GlnVal: 2.079 ± 0.05	0.286GlnTrp: 0.286 ± 0.016	1.323GlnTyr: 1.323 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	2.814ArgAla: 2.814 ± 0.055	0.617ArgCys: 0.617 ± 0.025	2.294ArgAsp: 2.294 ± 0.05	4.668ArgGlu: 4.668 ± 0.082	1.849ArgPhe: 1.849 ± 0.043	2.565ArgGly: 2.565 ± 0.054	0.751ArgHis: 0.751 ± 0.028	3.816ArgIle: 3.816 ± 0.054	3.972ArgLys: 3.972 ± 0.064	4.104ArgLeu: 4.104 ± 0.071	1.641ArgMet: 1.641 ± 0.038	2.177ArgAsn: 2.177 ± 0.054	1.292ArgPro: 1.292 ± 0.037	1.914ArgGln: 1.914 ± 0.055	2.465ArgArg: 2.465 ± 0.071	2.382ArgSer: 2.382 ± 0.053	2.21ArgThr: 2.21 ± 0.047	2.682ArgVal: 2.682 ± 0.055	0.36ArgTrp: 0.36 ± 0.018	1.938ArgTyr: 1.938 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	4.251SerAla: 4.251 ± 0.079	0.911SerCys: 0.911 ± 0.029	3.294SerAsp: 3.294 ± 0.059	3.971SerGlu: 3.971 ± 0.067	2.889SerPhe: 2.889 ± 0.051	4.869SerGly: 4.869 ± 0.071	1.049SerHis: 1.049 ± 0.032	3.97SerIle: 3.97 ± 0.058	2.933SerLys: 2.933 ± 0.05	5.543SerLeu: 5.543 ± 0.09	1.833SerMet: 1.833 ± 0.048	2.044SerAsn: 2.044 ± 0.049	1.689SerPro: 1.689 ± 0.042	1.704SerGln: 1.704 ± 0.044	2.893SerArg: 2.893 ± 0.059	3.506SerSer: 3.506 ± 0.075	2.757SerThr: 2.757 ± 0.062	4.364SerVal: 4.364 ± 0.066	0.526SerTrp: 0.526 ± 0.023	2.708SerTyr: 2.708 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	4.599ThrAla: 4.599 ± 0.076	0.689ThrCys: 0.689 ± 0.025	3.28ThrAsp: 3.28 ± 0.066	4.538ThrGlu: 4.538 ± 0.084	2.217ThrPhe: 2.217 ± 0.051	4.985ThrGly: 4.985 ± 0.075	0.787ThrHis: 0.787 ± 0.031	3.748ThrIle: 3.748 ± 0.066	2.699ThrLys: 2.699 ± 0.058	4.726ThrLeu: 4.726 ± 0.072	1.498ThrMet: 1.498 ± 0.035	1.665ThrAsn: 1.665 ± 0.047	1.995ThrPro: 1.995 ± 0.048	1.368ThrGln: 1.368 ± 0.04	2.048ThrArg: 2.048 ± 0.043	2.805ThrSer: 2.805 ± 0.055	2.69ThrThr: 2.69 ± 0.065	4.57ThrVal: 4.57 ± 0.081	0.46ThrTrp: 0.46 ± 0.022	2.092ThrTyr: 2.092 ± 0.045	0.001ThrXaa: 0.001 ± 0.001
Val	4.635ValAla: 4.635 ± 0.08	1.157ValCys: 1.157 ± 0.034	3.583ValAsp: 3.583 ± 0.067	4.455ValGlu: 4.455 ± 0.072	3.05ValPhe: 3.05 ± 0.059	3.93ValGly: 3.93 ± 0.08	1.034ValHis: 1.034 ± 0.033	5.43ValIle: 5.43 ± 0.082	4.181ValLys: 4.181 ± 0.065	6.867ValLeu: 6.867 ± 0.105	2.094ValMet: 2.094 ± 0.048	2.806ValAsn: 2.806 ± 0.055	2.466ValPro: 2.466 ± 0.05	1.936ValGln: 1.936 ± 0.043	3.069ValArg: 3.069 ± 0.054	4.554ValSer: 4.554 ± 0.064	4.172ValThr: 4.172 ± 0.066	4.493ValVal: 4.493 ± 0.08	0.577ValTrp: 0.577 ± 0.028	2.755ValTyr: 2.755 ± 0.051	0.0ValXaa: 0.0 ± 0.0
Trp	0.465TrpAla: 0.465 ± 0.022	0.148TrpCys: 0.148 ± 0.013	0.47TrpAsp: 0.47 ± 0.021	0.682TrpGlu: 0.682 ± 0.03	0.443TrpPhe: 0.443 ± 0.024	0.623TrpGly: 0.623 ± 0.026	0.187TrpHis: 0.187 ± 0.015	0.684TrpIle: 0.684 ± 0.027	0.702TrpLys: 0.702 ± 0.027	0.9TrpLeu: 0.9 ± 0.029	0.297TrpMet: 0.297 ± 0.018	0.499TrpAsn: 0.499 ± 0.023	0.236TrpPro: 0.236 ± 0.017	0.413TrpGln: 0.413 ± 0.019	0.321TrpArg: 0.321 ± 0.021	0.43TrpSer: 0.43 ± 0.024	0.384TrpThr: 0.384 ± 0.02	0.428TrpVal: 0.428 ± 0.02	0.111TrpTrp: 0.111 ± 0.01	0.391TrpTyr: 0.391 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.852TyrAla: 2.852 ± 0.059	0.65TyrCys: 0.65 ± 0.021	2.552TyrAsp: 2.552 ± 0.051	3.321TyrGlu: 3.321 ± 0.058	1.965TyrPhe: 1.965 ± 0.043	3.081TyrGly: 3.081 ± 0.052	0.986TyrHis: 0.986 ± 0.033	2.802TyrIle: 2.802 ± 0.058	2.065TyrLys: 2.065 ± 0.052	4.058TyrLeu: 4.058 ± 0.074	1.149TyrMet: 1.149 ± 0.038	1.786TyrAsn: 1.786 ± 0.045	1.501TyrPro: 1.501 ± 0.047	1.736TyrGln: 1.736 ± 0.047	2.253TyrArg: 2.253 ± 0.047	2.323TyrSer: 2.323 ± 0.053	2.283TyrThr: 2.283 ± 0.048	2.593TyrVal: 2.593 ± 0.056	0.389TyrTrp: 0.389 ± 0.021	1.99TyrTyr: 1.99 ± 0.051	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.002

Statistics based on 3107 proteins (999537 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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