Stemphylium lycopersici mycovirus
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346TJG4|A0A346TJG4_9VIRU Uncharacterized protein OS=Stemphylium lycopersici mycovirus OX=2499625 PE=4 SV=1
MM1 pKa = 7.32 FCDD4 pKa = 4.81 FEE6 pKa = 5.92 PDD8 pKa = 3.45 FPVFIPFLLRR18 pKa = 11.84 NVEE21 pKa = 4.12 SRR23 pKa = 11.84 VFSAPALLTAQASGGFGGPKK43 pKa = 9.44 SVRR46 pKa = 11.84 VSIAHH51 pKa = 6.49 FPLAAPDD58 pKa = 3.8 AEE60 pKa = 4.08 ALEE63 pKa = 4.74 EE64 pKa = 4.28 EE65 pKa = 4.98 SCSGTAGQDD74 pKa = 3.39 VFPVGVKK81 pKa = 8.99 STVWCGGAVCSGSCGFDD98 pKa = 3.51 GSCGISDD105 pKa = 3.23 VDD107 pKa = 3.87 EE108 pKa = 5.02 RR109 pKa = 11.84 GEE111 pKa = 4.5 GYY113 pKa = 9.91 FASTSFSSLVSVALAEE129 pKa = 4.0 LGYY132 pKa = 10.71 LGEE135 pKa = 4.26 LCRR138 pKa = 11.84 VQLSVGGMRR147 pKa = 11.84 LDD149 pKa = 3.33 ADD151 pKa = 3.81 FRR153 pKa = 11.84 GGARR157 pKa = 11.84 PQTTVFDD164 pKa = 4.25 AFAAEE169 pKa = 4.46 AFFPPSLEE177 pKa = 3.96 EE178 pKa = 4.04 VVVEE182 pKa = 4.61 DD183 pKa = 3.62 VAPAKK188 pKa = 10.57 VGAPADD194 pKa = 3.81 IPGSMPLDD202 pKa = 3.54 YY203 pKa = 10.8 VVSLAEE209 pKa = 4.46 TILPPAYY216 pKa = 10.51 VEE218 pKa = 4.01 AAKK221 pKa = 10.62 ADD223 pKa = 3.81 AVSLVPVSLPPPADD237 pKa = 3.72 GPVLGVDD244 pKa = 4.19 PGPAIPCVVNALFDD258 pKa = 3.98 CAEE261 pKa = 4.45 GVAQSTPGPVFAGDD275 pKa = 3.73 CDD277 pKa = 4.33 GEE279 pKa = 4.02 IDD281 pKa = 4.07 ARR283 pKa = 11.84 LAVLQAGGTLPRR295 pKa = 11.84 GCYY298 pKa = 10.3 SIVGDD303 pKa = 4.24 HH304 pKa = 5.95 NWFNILRR311 pKa = 11.84 GLARR315 pKa = 11.84 WFASSGVSVRR325 pKa = 11.84 GARR328 pKa = 11.84 MDD330 pKa = 3.91 LLLGGLSVARR340 pKa = 11.84 DD341 pKa = 3.37 DD342 pKa = 3.9 RR343 pKa = 11.84 LRR345 pKa = 11.84 ALRR348 pKa = 11.84 LLRR351 pKa = 11.84 FLGLGRR357 pKa = 11.84 GGDD360 pKa = 3.6 EE361 pKa = 4.17 AGVGDD366 pKa = 4.15 RR367 pKa = 11.84 VLFPPCARR375 pKa = 11.84 AVVDD379 pKa = 4.19 ARR381 pKa = 11.84 LAYY384 pKa = 9.9 LVWDD388 pKa = 4.74 WIGGSSAADD397 pKa = 3.12
Molecular weight: 41.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.341
IPC2_protein 4.304
IPC_protein 4.279
Toseland 4.088
ProMoST 4.38
Dawson 4.253
Bjellqvist 4.418
Wikipedia 4.164
Rodwell 4.113
Grimsley 3.986
Solomon 4.253
Lehninger 4.202
Nozaki 4.355
DTASelect 4.584
Thurlkill 4.113
EMBOSS 4.177
Sillero 4.393
Patrickios 3.605
IPC_peptide 4.253
IPC2_peptide 4.38
IPC2.peptide.svr19 4.322
Protein with the highest isoelectric point:
>tr|A0A346TJG2|A0A346TJG2_9VIRU Coat protein OS=Stemphylium lycopersici mycovirus OX=2499625 PE=4 SV=1
MM1 pKa = 7.77 RR2 pKa = 11.84 SDD4 pKa = 4.32 KK5 pKa = 11.17 SLLLLGFRR13 pKa = 11.84 NAGWLRR19 pKa = 11.84 AQGEE23 pKa = 4.53 HH24 pKa = 7.33 PDD26 pKa = 3.74 LGAIPKK32 pKa = 9.82 RR33 pKa = 11.84 AFVPEE38 pKa = 4.18 PEE40 pKa = 4.66 EE41 pKa = 4.21 VDD43 pKa = 3.31 ACLKK47 pKa = 10.66 GIEE50 pKa = 4.9 DD51 pKa = 3.7 GWGDD55 pKa = 3.62 GAFWCPTVIGSAFPVFFVLSNEE77 pKa = 3.84 TRR79 pKa = 11.84 NAFCHH84 pKa = 6.03 SFSSVGGDD92 pKa = 3.66 VNWLTFGLTDD102 pKa = 4.82 ARR104 pKa = 11.84 TLLYY108 pKa = 10.14 RR109 pKa = 11.84 RR110 pKa = 11.84 PQVCVVAKK118 pKa = 9.45 PRR120 pKa = 11.84 PCVAYY125 pKa = 10.34 HH126 pKa = 7.1 RR127 pKa = 11.84 DD128 pKa = 3.27 LSSIARR134 pKa = 11.84 VLRR137 pKa = 11.84 YY138 pKa = 9.55 GVLNSDD144 pKa = 4.59 HH145 pKa = 6.98 KK146 pKa = 11.5 LCDD149 pKa = 3.33 AYY151 pKa = 10.06 TDD153 pKa = 4.46 FVRR156 pKa = 11.84 SPVARR161 pKa = 11.84 TEE163 pKa = 4.35 EE164 pKa = 3.94 VWGTLGAYY172 pKa = 8.52 PWGDD176 pKa = 3.84 LDD178 pKa = 4.03 TLVGQSTRR186 pKa = 11.84 AEE188 pKa = 3.82 YY189 pKa = 10.75 DD190 pKa = 3.2 AVKK193 pKa = 10.47 DD194 pKa = 3.55 AAVVGFLDD202 pKa = 4.04 RR203 pKa = 11.84 LWGKK207 pKa = 10.21 KK208 pKa = 9.15 RR209 pKa = 11.84 GKK211 pKa = 9.74 GAPRR215 pKa = 11.84 DD216 pKa = 3.38 RR217 pKa = 11.84 KK218 pKa = 10.16 VRR220 pKa = 11.84 AKK222 pKa = 10.82 AAMAWGDD229 pKa = 3.14 ARR231 pKa = 11.84 LWSRR235 pKa = 11.84 EE236 pKa = 3.58 VSFKK240 pKa = 10.33 RR241 pKa = 11.84 RR242 pKa = 11.84 HH243 pKa = 5.67 YY244 pKa = 10.58 YY245 pKa = 10.41 DD246 pKa = 3.03 VATFLCVMDD255 pKa = 4.53 EE256 pKa = 4.26 VWGSRR261 pKa = 11.84 DD262 pKa = 3.05 VALRR266 pKa = 11.84 GGATNAEE273 pKa = 4.37 AGLQVGSFRR282 pKa = 11.84 KK283 pKa = 9.29 AVRR286 pKa = 11.84 MVLSEE291 pKa = 4.42 LVMGPASYY299 pKa = 10.63 EE300 pKa = 3.8 RR301 pKa = 11.84 LYY303 pKa = 10.6 LTIFWVFSTRR313 pKa = 11.84 WWPSLVPVLIRR324 pKa = 11.84 YY325 pKa = 7.18 GTLGMSDD332 pKa = 4.34 DD333 pKa = 4.98 EE334 pKa = 4.24 YY335 pKa = 10.98 TAIHH339 pKa = 6.54 KK340 pKa = 8.84 EE341 pKa = 3.35 ITAVVTSTWMVPGTCRR357 pKa = 11.84 QHH359 pKa = 5.91 QFSANFLNAEE369 pKa = 4.57 DD370 pKa = 3.7 LTGWSDD376 pKa = 3.47 RR377 pKa = 11.84 DD378 pKa = 3.67 SLKK381 pKa = 11.22 GGVCTEE387 pKa = 4.36 IIKK390 pKa = 10.32 FALATFEE397 pKa = 3.79 YY398 pKa = 10.21 RR399 pKa = 11.84 ADD401 pKa = 3.47 VRR403 pKa = 11.84 GGGGEE408 pKa = 3.66 RR409 pKa = 11.84 GMPIIAKK416 pKa = 9.13 EE417 pKa = 4.15 GSPSEE422 pKa = 3.92 YY423 pKa = 9.89 LSRR426 pKa = 11.84 YY427 pKa = 8.1 RR428 pKa = 11.84 AAMDD432 pKa = 3.2 QLLRR436 pKa = 11.84 PMYY439 pKa = 10.54 AKK441 pKa = 10.69 YY442 pKa = 9.87 SANMTDD448 pKa = 4.11 LRR450 pKa = 11.84 SHH452 pKa = 5.46 MEE454 pKa = 4.04 KK455 pKa = 10.46 RR456 pKa = 11.84 MAWMSGGAVGRR467 pKa = 11.84 RR468 pKa = 11.84 AKK470 pKa = 10.32 EE471 pKa = 3.52 LLGPGIAPPGSSKK484 pKa = 11.08 AYY486 pKa = 8.05 VASRR490 pKa = 11.84 ADD492 pKa = 3.6 ADD494 pKa = 3.57 QLTRR498 pKa = 11.84 DD499 pKa = 3.45 WGEE502 pKa = 3.46 MRR504 pKa = 11.84 IEE506 pKa = 3.98 VGGKK510 pKa = 8.57 GNEE513 pKa = 4.03 RR514 pKa = 11.84 GSEE517 pKa = 3.96 RR518 pKa = 11.84 TLLATDD524 pKa = 4.29 LRR526 pKa = 11.84 DD527 pKa = 3.66 QVSEE531 pKa = 4.39 SYY533 pKa = 10.83 LLHH536 pKa = 6.56 AFKK539 pKa = 10.77 NRR541 pKa = 11.84 YY542 pKa = 8.45 GLIGVDD548 pKa = 3.33 VGEE551 pKa = 4.82 TPVEE555 pKa = 3.84 MFKK558 pKa = 10.72 RR559 pKa = 11.84 HH560 pKa = 4.39 VTVASATDD568 pKa = 3.54 VPMSFHH574 pKa = 6.04 GRR576 pKa = 11.84 EE577 pKa = 4.17 KK578 pKa = 10.67 KK579 pKa = 10.54 VLAAWDD585 pKa = 3.71 YY586 pKa = 11.34 SKK588 pKa = 10.67 WDD590 pKa = 3.49 HH591 pKa = 6.43 HH592 pKa = 7.34 VMLAEE597 pKa = 4.26 RR598 pKa = 11.84 LLLVQVMRR606 pKa = 11.84 DD607 pKa = 3.31 LVLEE611 pKa = 4.05 YY612 pKa = 10.61 VQRR615 pKa = 11.84 DD616 pKa = 4.26 DD617 pKa = 5.05 IRR619 pKa = 11.84 VDD621 pKa = 3.28 MLKK624 pKa = 10.45 EE625 pKa = 3.87 LDD627 pKa = 3.77 VLEE630 pKa = 4.61 SSHH633 pKa = 6.51 RR634 pKa = 11.84 VAIYY638 pKa = 10.22 RR639 pKa = 11.84 SRR641 pKa = 11.84 AYY643 pKa = 10.61 ADD645 pKa = 3.36 ARR647 pKa = 11.84 YY648 pKa = 7.8 TDD650 pKa = 3.58 QVDD653 pKa = 3.02 ALIRR657 pKa = 11.84 DD658 pKa = 3.91 GVKK661 pKa = 10.55 AGFKK665 pKa = 9.59 GQAVRR670 pKa = 11.84 LSADD674 pKa = 3.22 QVRR677 pKa = 11.84 ITDD680 pKa = 3.53 YY681 pKa = 11.29 AGQQSGRR688 pKa = 11.84 RR689 pKa = 11.84 STLEE693 pKa = 3.6 SNTFYY698 pKa = 11.23 SRR700 pKa = 11.84 ARR702 pKa = 11.84 LLVRR706 pKa = 11.84 DD707 pKa = 3.98 AEE709 pKa = 4.25 LAEE712 pKa = 4.55 GEE714 pKa = 4.13 RR715 pKa = 11.84 SVYY718 pKa = 10.47 LLNRR722 pKa = 11.84 ADD724 pKa = 5.15 DD725 pKa = 3.64 VLEE728 pKa = 4.28 VYY730 pKa = 10.17 RR731 pKa = 11.84 AWEE734 pKa = 3.88 HH735 pKa = 5.7 ARR737 pKa = 11.84 NAIDD741 pKa = 4.24 VMLLQGHH748 pKa = 6.65 KK749 pKa = 10.49 ANKK752 pKa = 8.77 KK753 pKa = 7.94 KK754 pKa = 10.5 QVVQVRR760 pKa = 11.84 TGVYY764 pKa = 10.06 FRR766 pKa = 11.84 ILYY769 pKa = 9.9 AAGSMRR775 pKa = 11.84 GFPPRR780 pKa = 11.84 AVYY783 pKa = 10.5 ACASAGPSMAASGGFDD799 pKa = 3.15 PVEE802 pKa = 4.21 RR803 pKa = 11.84 LSSLSGALDD812 pKa = 3.24 RR813 pKa = 11.84 LARR816 pKa = 11.84 RR817 pKa = 11.84 GSGYY821 pKa = 8.88 WVARR825 pKa = 11.84 ALYY828 pKa = 10.44 LEE830 pKa = 4.67 AEE832 pKa = 4.39 DD833 pKa = 4.46 YY834 pKa = 10.92 YY835 pKa = 11.5 RR836 pKa = 11.84 DD837 pKa = 3.5 VRR839 pKa = 11.84 VQLAVKK845 pKa = 9.87 ADD847 pKa = 2.97 KK848 pKa = 9.19 FTRR851 pKa = 11.84 FTIPRR856 pKa = 11.84 EE857 pKa = 4.02 VLRR860 pKa = 11.84 ASPDD864 pKa = 3.31 LGGCGVLPPGNYY876 pKa = 10.44 DD877 pKa = 3.08 FDD879 pKa = 5.82 LSIKK883 pKa = 10.13 CSEE886 pKa = 3.95 IVGEE890 pKa = 5.54 NGRR893 pKa = 11.84 LWQAVRR899 pKa = 11.84 EE900 pKa = 4.14 RR901 pKa = 11.84 LEE903 pKa = 3.86 KK904 pKa = 9.38 RR905 pKa = 11.84 HH906 pKa = 5.91 KK907 pKa = 10.51 FRR909 pKa = 11.84 GLADD913 pKa = 3.78 LQGSAGRR920 pKa = 11.84 AFSHH924 pKa = 7.05 DD925 pKa = 3.53 VPLDD929 pKa = 3.67 VPEE932 pKa = 4.91 KK933 pKa = 10.58 AWQQWQKK940 pKa = 9.36 RR941 pKa = 11.84 WRR943 pKa = 11.84 GDD945 pKa = 2.84 RR946 pKa = 11.84 AAQDD950 pKa = 3.45 GRR952 pKa = 11.84 GEE954 pKa = 4.18 LLRR957 pKa = 11.84 ARR959 pKa = 11.84 LMGKK963 pKa = 8.43 LWRR966 pKa = 11.84 YY967 pKa = 7.77 KK968 pKa = 10.43 RR969 pKa = 11.84 SWHH972 pKa = 6.93 RR973 pKa = 11.84 DD974 pKa = 3.06 RR975 pKa = 11.84 KK976 pKa = 9.24 GDD978 pKa = 3.2 AWGLEE983 pKa = 4.4 GFGHH987 pKa = 7.59 LLSLPVDD994 pKa = 4.3 FFQLSWRR1001 pKa = 11.84 ILRR1004 pKa = 11.84 DD1005 pKa = 3.61 CPGSGEE1011 pKa = 4.17 VDD1013 pKa = 2.9 RR1014 pKa = 11.84 LMKK1017 pKa = 10.44 GVQGPPAEE1025 pKa = 4.31 GMLAKK1030 pKa = 10.37 LWYY1033 pKa = 10.47 GLGQDD1038 pKa = 4.1 VISVLGRR1045 pKa = 11.84 DD1046 pKa = 3.64 VLEE1049 pKa = 4.88 VIQSASAPGRR1059 pKa = 11.84 EE1060 pKa = 4.98 FAAEE1064 pKa = 4.12 WGWLPLDD1071 pKa = 3.56 VRR1073 pKa = 11.84 EE1074 pKa = 4.15 KK1075 pKa = 10.64 FLRR1078 pKa = 11.84 GHH1080 pKa = 7.52 LGPAGAWMNLMPASFAPWLNQAVNFVLFQAFATGVRR1116 pKa = 11.84 YY1117 pKa = 9.66 HH1118 pKa = 6.69 GSHH1121 pKa = 6.61 WLLHH1125 pKa = 5.2 VRR1127 pKa = 11.84 QALTGAITRR1136 pKa = 11.84 QFVDD1140 pKa = 3.27 ARR1142 pKa = 11.84 PLVVLHH1148 pKa = 6.72
Molecular weight: 128.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.774
IPC2_protein 8.697
IPC_protein 8.653
Toseland 9.136
ProMoST 9.092
Dawson 9.502
Bjellqvist 9.341
Wikipedia 9.721
Rodwell 9.619
Grimsley 9.589
Solomon 9.531
Lehninger 9.472
Nozaki 9.385
DTASelect 9.268
Thurlkill 9.355
EMBOSS 9.619
Sillero 9.502
Patrickios 4.8
IPC_peptide 9.516
IPC2_peptide 8.112
IPC2.peptide.svr19 7.735
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
3143
397
1148
785.8
85.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.581 ± 0.836
1.336 ± 0.309
6.841 ± 0.178
5.154 ± 0.349
3.754 ± 0.401
11.04 ± 0.843
1.973 ± 0.272
2.195 ± 0.139
2.609 ± 0.689
9.8 ± 0.572
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.004 ± 0.229
1.75 ± 0.148
5.218 ± 0.824
2.195 ± 0.349
8.559 ± 0.649
6.109 ± 0.324
3.532 ± 0.248
9.131 ± 0.388
2.418 ± 0.31
2.8 ± 0.393
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here