Altererythrobacter xixiisoli
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3571 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6I4U0H3|A0A6I4U0H3_9SPHN Nuclear transport factor 2 family protein OS=Altererythrobacter xixiisoli OX=1476466 GN=GRI97_15310 PE=4 SV=1
MM1 pKa = 7.39 NGIKK5 pKa = 10.03 FVGAMCVSVMACGVIASPSHH25 pKa = 5.77 AQDD28 pKa = 3.33 AAVEE32 pKa = 4.36 GADD35 pKa = 3.59 SGSVTVNGSVTLASDD50 pKa = 3.34 YY51 pKa = 10.82 RR52 pKa = 11.84 FRR54 pKa = 11.84 GVSQTDD60 pKa = 3.08 GNMTVQGGLTVSHH73 pKa = 7.68 DD74 pKa = 3.45 SGLYY78 pKa = 9.35 AGAWASNLAGWGTFGGANMEE98 pKa = 4.36 LDD100 pKa = 3.7 LLLGFKK106 pKa = 10.62 ADD108 pKa = 3.87 LGGGAMLDD116 pKa = 3.7 TGLIWYY122 pKa = 7.52 MYY124 pKa = 9.96 PGGADD129 pKa = 3.18 DD130 pKa = 4.14 TDD132 pKa = 3.69 FAEE135 pKa = 5.38 IYY137 pKa = 10.44 AKK139 pKa = 10.79 LSGTTGPLTLTGGVQYY155 pKa = 11.06 APKK158 pKa = 10.14 QYY160 pKa = 11.71 ALANVSNAPDD170 pKa = 3.48 SAGQTQDD177 pKa = 3.19 NLYY180 pKa = 10.69 LFADD184 pKa = 4.22 GLMAVPEE191 pKa = 4.38 TPFTVKK197 pKa = 10.61 AHH199 pKa = 6.41 IGYY202 pKa = 9.94 SDD204 pKa = 3.96 GNPGLGPNGTSVSPTGSYY222 pKa = 8.95 WDD224 pKa = 3.32 WMLGVDD230 pKa = 3.95 TSWRR234 pKa = 11.84 NLTLGVAYY242 pKa = 10.58 VDD244 pKa = 3.75 TDD246 pKa = 3.2 ISDD249 pKa = 4.11 AEE251 pKa = 4.21 ALRR254 pKa = 11.84 LQPNFSKK261 pKa = 11.12 GQDD264 pKa = 3.5 GSGSIADD271 pKa = 3.79 GTVVLSLTAAFF282 pKa = 4.57
Molecular weight: 28.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.91
IPC_protein 3.935
Toseland 3.694
ProMoST 4.126
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.923
Rodwell 3.757
Grimsley 3.605
Solomon 3.935
Lehninger 3.897
Nozaki 4.062
DTASelect 4.368
Thurlkill 3.77
EMBOSS 3.923
Sillero 4.062
Patrickios 0.846
IPC_peptide 3.935
IPC2_peptide 4.037
IPC2.peptide.svr19 3.927
Protein with the highest isoelectric point:
>tr|A0A6I4TRC8|A0A6I4TRC8_9SPHN HlyD family efflux transporter periplasmic adaptor subunit OS=Altererythrobacter xixiisoli OX=1476466 GN=GRI97_01640 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.24 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.93 GFFARR21 pKa = 11.84 KK22 pKa = 7.44 ATPGGRR28 pKa = 11.84 NVLRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.02 KK41 pKa = 10.61 LSAA44 pKa = 4.03
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3571
0
3571
1157999
29
4215
324.3
35.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.98 ± 0.075
0.8 ± 0.013
6.161 ± 0.051
5.349 ± 0.035
3.597 ± 0.028
8.91 ± 0.051
1.966 ± 0.022
5.068 ± 0.025
2.489 ± 0.034
9.956 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.469 ± 0.022
2.767 ± 0.035
5.369 ± 0.036
3.593 ± 0.024
7.258 ± 0.043
5.394 ± 0.037
5.267 ± 0.037
6.946 ± 0.034
1.461 ± 0.017
2.201 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here