Streptomyces sp. DSM 15324

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7596 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A101UJI4|A0A101UJI4_9ACTN LuxR family transcriptional regulator OS=Streptomyces sp. DSM 15324 OX=1739111 GN=AQJ58_11835 PE=4 SV=1
MM1 pKa = 7.81SIPPPPGSPQPQDD14 pKa = 3.75PYY16 pKa = 10.94RR17 pKa = 11.84PPPPHH22 pKa = 6.68GPYY25 pKa = 9.86PQGPYY30 pKa = 9.24PQAPQAPYY38 pKa = 10.23GAVPGPYY45 pKa = 9.14QVWGQGYY52 pKa = 9.65SPLARR57 pKa = 11.84PAPVNGVAIAALVLGLLCFLPAVGLILGIIALVQIKK93 pKa = 10.33KK94 pKa = 10.08RR95 pKa = 11.84GEE97 pKa = 3.94RR98 pKa = 11.84GKK100 pKa = 11.49GMAIAGAVVSSLGLALWALSLSTNVASEE128 pKa = 3.77FWEE131 pKa = 4.88GVKK134 pKa = 10.44EE135 pKa = 4.05GARR138 pKa = 11.84GGGYY142 pKa = 10.08SLAAGDD148 pKa = 4.67CFDD151 pKa = 4.38VPGSFDD157 pKa = 4.07RR158 pKa = 11.84DD159 pKa = 3.64VYY161 pKa = 11.47DD162 pKa = 3.55VDD164 pKa = 4.94EE165 pKa = 4.98VPCSDD170 pKa = 3.39PHH172 pKa = 7.5EE173 pKa = 4.91GEE175 pKa = 4.25AFGTIPLSGDD185 pKa = 3.61DD186 pKa = 4.19YY187 pKa = 11.49PGDD190 pKa = 4.16GYY192 pKa = 10.48VTDD195 pKa = 3.92QAEE198 pKa = 4.64DD199 pKa = 3.24KK200 pKa = 11.04CGPLQDD206 pKa = 4.48AYY208 pKa = 11.1AMDD211 pKa = 3.97RR212 pKa = 11.84WALGDD217 pKa = 3.88EE218 pKa = 4.08VDD220 pKa = 4.58VYY222 pKa = 11.65YY223 pKa = 9.37LTPTADD229 pKa = 2.76SWEE232 pKa = 4.06WGDD235 pKa = 5.2RR236 pKa = 11.84EE237 pKa = 4.28ITCVFAHH244 pKa = 6.29TDD246 pKa = 3.31EE247 pKa = 4.89TGTLTGSLRR256 pKa = 11.84ADD258 pKa = 3.76EE259 pKa = 4.48TTLDD263 pKa = 3.8ADD265 pKa = 3.75QMAFLKK271 pKa = 10.91AMAAIDD277 pKa = 3.61DD278 pKa = 4.11VLYY281 pKa = 10.74EE282 pKa = 4.36EE283 pKa = 5.35PDD285 pKa = 3.82DD286 pKa = 4.08YY287 pKa = 12.17AEE289 pKa = 5.63ADD291 pKa = 3.44LDD293 pKa = 4.92ANKK296 pKa = 10.59DD297 pKa = 2.98WAADD301 pKa = 3.58VRR303 pKa = 11.84DD304 pKa = 3.98VLAEE308 pKa = 3.76QAGALDD314 pKa = 3.5AHH316 pKa = 6.3TWSGDD321 pKa = 3.04AGKK324 pKa = 10.5SVDD327 pKa = 3.35ALVRR331 pKa = 11.84EE332 pKa = 4.36MRR334 pKa = 11.84DD335 pKa = 4.04ARR337 pKa = 11.84ADD339 pKa = 3.24WAKK342 pKa = 10.84AATAGDD348 pKa = 3.85ADD350 pKa = 4.11TFYY353 pKa = 11.54LHH355 pKa = 6.69YY356 pKa = 9.89EE357 pKa = 3.7SGYY360 pKa = 11.11AYY362 pKa = 10.94VDD364 pKa = 3.21GDD366 pKa = 3.88TTVAARR372 pKa = 11.84KK373 pKa = 9.67ALDD376 pKa = 3.7LATTPPSYY384 pKa = 11.2DD385 pKa = 3.45EE386 pKa = 5.57DD387 pKa = 3.96EE388 pKa = 4.95GDD390 pKa = 4.28GGDD393 pKa = 3.73SGSGSGSGSGSGGSGSEE410 pKa = 4.34DD411 pKa = 3.24VV412 pKa = 4.05

Molecular weight: 43.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A101UDP8|A0A101UDP8_9ACTN DNA polymerase III subunit gamma/tau OS=Streptomyces sp. DSM 15324 OX=1739111 GN=AQJ58_29525 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7596

0

7596

2550604

29

5257

335.8

35.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.651 ± 0.045

0.793 ± 0.007

6.014 ± 0.022

5.62 ± 0.032

2.714 ± 0.016

9.511 ± 0.029

2.34 ± 0.016

3.001 ± 0.018

2.056 ± 0.023

10.45 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.646 ± 0.012

1.715 ± 0.016

6.127 ± 0.029

2.703 ± 0.016

8.144 ± 0.035

4.999 ± 0.018

6.28 ± 0.026

8.601 ± 0.024

1.52 ± 0.012

2.114 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski