Amino acid dipepetide frequency for Streptomyces sp. DSM 15324

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.453AlaAla: 21.453 ± 0.156
1.093AlaCys: 1.093 ± 0.023
8.395AlaAsp: 8.395 ± 0.069
8.785AlaGlu: 8.785 ± 0.092
3.5AlaPhe: 3.5 ± 0.038
13.083AlaGly: 13.083 ± 0.106
3.044AlaHis: 3.044 ± 0.036
3.203AlaIle: 3.203 ± 0.044
2.705AlaLys: 2.705 ± 0.049
14.947AlaLeu: 14.947 ± 0.106
2.304AlaMet: 2.304 ± 0.032
1.895AlaAsn: 1.895 ± 0.028
7.241AlaPro: 7.241 ± 0.074
3.833AlaGln: 3.833 ± 0.043
10.593AlaArg: 10.593 ± 0.078
5.995AlaSer: 5.995 ± 0.059
7.083AlaThr: 7.083 ± 0.064
12.671AlaVal: 12.671 ± 0.087
1.845AlaTrp: 1.845 ± 0.03
2.846AlaTyr: 2.846 ± 0.037
0.0AlaXaa: 0.0 ± 0.0
Cys
1.154CysAla: 1.154 ± 0.023
0.09CysCys: 0.09 ± 0.007
0.47CysAsp: 0.47 ± 0.014
0.395CysGlu: 0.395 ± 0.013
0.214CysPhe: 0.214 ± 0.008
0.954CysGly: 0.954 ± 0.02
0.205CysHis: 0.205 ± 0.008
0.146CysIle: 0.146 ± 0.008
0.108CysLys: 0.108 ± 0.006
0.777CysLeu: 0.777 ± 0.018
0.122CysMet: 0.122 ± 0.007
0.124CysAsn: 0.124 ± 0.007
0.478CysPro: 0.478 ± 0.014
0.156CysGln: 0.156 ± 0.008
0.627CysArg: 0.627 ± 0.017
0.423CysSer: 0.423 ± 0.016
0.51CysThr: 0.51 ± 0.014
0.7CysVal: 0.7 ± 0.017
0.121CysTrp: 0.121 ± 0.007
0.151CysTyr: 0.151 ± 0.007
0.0CysXaa: 0.0 ± 0.0
Asp
7.617AspAla: 7.617 ± 0.062
0.436AspCys: 0.436 ± 0.014
3.683AspAsp: 3.683 ± 0.043
3.777AspGlu: 3.777 ± 0.042
1.685AspPhe: 1.685 ± 0.029
6.516AspGly: 6.516 ± 0.06
1.46AspHis: 1.46 ± 0.026
1.858AspIle: 1.858 ± 0.027
1.124AspLys: 1.124 ± 0.025
6.424AspLeu: 6.424 ± 0.058
0.776AspMet: 0.776 ± 0.016
0.955AspAsn: 0.955 ± 0.022
4.56AspPro: 4.56 ± 0.043
1.478AspGln: 1.478 ± 0.025
5.018AspArg: 5.018 ± 0.052
2.519AspSer: 2.519 ± 0.037
3.364AspThr: 3.364 ± 0.041
4.751AspVal: 4.751 ± 0.045
1.037AspTrp: 1.037 ± 0.022
1.105AspTyr: 1.105 ± 0.023
0.0AspXaa: 0.0 ± 0.0
Glu
7.382GluAla: 7.382 ± 0.072
0.359GluCys: 0.359 ± 0.014
2.892GluAsp: 2.892 ± 0.035
3.612GluGlu: 3.612 ± 0.048
1.429GluPhe: 1.429 ± 0.023
4.372GluGly: 4.372 ± 0.037
1.506GluHis: 1.506 ± 0.023
2.236GluIle: 2.236 ± 0.028
1.364GluLys: 1.364 ± 0.029
6.592GluLeu: 6.592 ± 0.057
0.842GluMet: 0.842 ± 0.021
1.024GluAsn: 1.024 ± 0.023
3.376GluPro: 3.376 ± 0.043
2.137GluGln: 2.137 ± 0.032
5.426GluArg: 5.426 ± 0.059
2.464GluSer: 2.464 ± 0.035
2.827GluThr: 2.827 ± 0.036
4.51GluVal: 4.51 ± 0.039
0.761GluTrp: 0.761 ± 0.019
1.089GluTyr: 1.089 ± 0.021
0.0GluXaa: 0.0 ± 0.0
Phe
3.649PheAla: 3.649 ± 0.041
0.253PheCys: 0.253 ± 0.011
1.939PheAsp: 1.939 ± 0.029
1.396PheGlu: 1.396 ± 0.022
0.87PhePhe: 0.87 ± 0.02
2.983PheGly: 2.983 ± 0.034
0.614PheHis: 0.614 ± 0.017
0.648PheIle: 0.648 ± 0.017
0.498PheLys: 0.498 ± 0.015
2.666PheLeu: 2.666 ± 0.038
0.381PheMet: 0.381 ± 0.014
0.538PheAsn: 0.538 ± 0.015
1.397PhePro: 1.397 ± 0.023
0.656PheGln: 0.656 ± 0.017
1.877PheArg: 1.877 ± 0.029
1.481PheSer: 1.481 ± 0.024
2.031PheThr: 2.031 ± 0.031
2.254PheVal: 2.254 ± 0.03
0.429PheTrp: 0.429 ± 0.013
0.584PheTyr: 0.584 ± 0.016
0.0PheXaa: 0.0 ± 0.0
Gly
11.368GlyAla: 11.368 ± 0.081
0.837GlyCys: 0.837 ± 0.019
5.26GlyAsp: 5.26 ± 0.046
5.118GlyGlu: 5.118 ± 0.045
2.857GlyPhe: 2.857 ± 0.038
8.987GlyGly: 8.987 ± 0.095
2.374GlyHis: 2.374 ± 0.033
3.311GlyIle: 3.311 ± 0.04
2.301GlyLys: 2.301 ± 0.041
9.524GlyLeu: 9.524 ± 0.067
1.926GlyMet: 1.926 ± 0.024
1.725GlyAsn: 1.725 ± 0.033
5.232GlyPro: 5.232 ± 0.064
2.575GlyGln: 2.575 ± 0.039
7.954GlyArg: 7.954 ± 0.071
5.334GlySer: 5.334 ± 0.057
6.729GlyThr: 6.729 ± 0.065
7.73GlyVal: 7.73 ± 0.06
1.688GlyTrp: 1.688 ± 0.03
2.281GlyTyr: 2.281 ± 0.029
0.0GlyXaa: 0.0 ± 0.0
His
2.801HisAla: 2.801 ± 0.035
0.209HisCys: 0.209 ± 0.009
1.346HisAsp: 1.346 ± 0.025
1.201HisGlu: 1.201 ± 0.024
0.662HisPhe: 0.662 ± 0.017
2.499HisGly: 2.499 ± 0.034
0.697HisHis: 0.697 ± 0.017
0.665HisIle: 0.665 ± 0.016
0.338HisLys: 0.338 ± 0.013
2.506HisLeu: 2.506 ± 0.034
0.307HisMet: 0.307 ± 0.011
0.364HisAsn: 0.364 ± 0.013
1.891HisPro: 1.891 ± 0.031
0.654HisGln: 0.654 ± 0.016
2.18HisArg: 2.18 ± 0.03
1.018HisSer: 1.018 ± 0.021
1.433HisThr: 1.433 ± 0.024
1.738HisVal: 1.738 ± 0.03
0.392HisTrp: 0.392 ± 0.012
0.499HisTyr: 0.499 ± 0.013
0.0HisXaa: 0.0 ± 0.0
Ile
4.455IleAla: 4.455 ± 0.045
0.271IleCys: 0.271 ± 0.01
2.149IleAsp: 2.149 ± 0.035
1.87IleGlu: 1.87 ± 0.029
0.645IlePhe: 0.645 ± 0.019
3.385IleGly: 3.385 ± 0.044
0.585IleHis: 0.585 ± 0.014
0.781IleIle: 0.781 ± 0.022
0.684IleLys: 0.684 ± 0.02
2.33IleLeu: 2.33 ± 0.033
0.43IleMet: 0.43 ± 0.013
0.632IleAsn: 0.632 ± 0.015
1.703IlePro: 1.703 ± 0.029
0.696IleGln: 0.696 ± 0.018
2.214IleArg: 2.214 ± 0.033
1.568IleSer: 1.568 ± 0.026
2.167IleThr: 2.167 ± 0.029
2.601IleVal: 2.601 ± 0.035
0.351IleTrp: 0.351 ± 0.011
0.497IleTyr: 0.497 ± 0.016
0.0IleXaa: 0.0 ± 0.0
Lys
2.89LysAla: 2.89 ± 0.054
0.1LysCys: 0.1 ± 0.007
1.319LysAsp: 1.319 ± 0.032
1.151LysGlu: 1.151 ± 0.025
0.428LysPhe: 0.428 ± 0.014
1.777LysGly: 1.777 ± 0.036
0.397LysHis: 0.397 ± 0.012
0.819LysIle: 0.819 ± 0.019
0.809LysLys: 0.809 ± 0.025
1.894LysLeu: 1.894 ± 0.037
0.356LysMet: 0.356 ± 0.013
0.494LysAsn: 0.494 ± 0.016
1.234LysPro: 1.234 ± 0.028
0.659LysGln: 0.659 ± 0.018
1.342LysArg: 1.342 ± 0.025
1.066LysSer: 1.066 ± 0.021
1.253LysThr: 1.253 ± 0.023
1.868LysVal: 1.868 ± 0.033
0.264LysTrp: 0.264 ± 0.01
0.44LysTyr: 0.44 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
15.334LeuAla: 15.334 ± 0.088
0.858LeuCys: 0.858 ± 0.019
6.796LeuAsp: 6.796 ± 0.053
4.695LeuGlu: 4.695 ± 0.05
2.6LeuPhe: 2.6 ± 0.034
9.443LeuGly: 9.443 ± 0.072
2.396LeuHis: 2.396 ± 0.029
3.097LeuIle: 3.097 ± 0.04
2.029LeuLys: 2.029 ± 0.038
11.538LeuLeu: 11.538 ± 0.086
1.593LeuMet: 1.593 ± 0.026
1.618LeuAsn: 1.618 ± 0.028
6.623LeuPro: 6.623 ± 0.056
2.143LeuGln: 2.143 ± 0.032
8.955LeuArg: 8.955 ± 0.063
5.291LeuSer: 5.291 ± 0.051
7.287LeuThr: 7.287 ± 0.059
8.941LeuVal: 8.941 ± 0.076
1.344LeuTrp: 1.344 ± 0.026
1.918LeuTyr: 1.918 ± 0.03
0.0LeuXaa: 0.0 ± 0.0
Met
2.165MetAla: 2.165 ± 0.028
0.135MetCys: 0.135 ± 0.008
0.861MetAsp: 0.861 ± 0.02
0.703MetGlu: 0.703 ± 0.019
0.431MetPhe: 0.431 ± 0.014
1.249MetGly: 1.249 ± 0.023
0.345MetHis: 0.345 ± 0.012
0.639MetIle: 0.639 ± 0.018
0.385MetLys: 0.385 ± 0.013
1.557MetLeu: 1.557 ± 0.026
0.27MetMet: 0.27 ± 0.011
0.405MetAsn: 0.405 ± 0.013
1.059MetPro: 1.059 ± 0.02
0.436MetGln: 0.436 ± 0.013
1.333MetArg: 1.333 ± 0.025
1.266MetSer: 1.266 ± 0.02
1.518MetThr: 1.518 ± 0.024
1.21MetVal: 1.21 ± 0.022
0.196MetTrp: 0.196 ± 0.008
0.304MetTyr: 0.304 ± 0.01
0.0MetXaa: 0.0 ± 0.0
Asn
2.134AsnAla: 2.134 ± 0.035
0.168AsnCys: 0.168 ± 0.009
0.917AsnAsp: 0.917 ± 0.019
0.766AsnGlu: 0.766 ± 0.017
0.486AsnPhe: 0.486 ± 0.016
1.883AsnGly: 1.883 ± 0.035
0.38AsnHis: 0.38 ± 0.013
0.603AsnIle: 0.603 ± 0.016
0.382AsnLys: 0.382 ± 0.013
1.611AsnLeu: 1.611 ± 0.03
0.287AsnMet: 0.287 ± 0.011
0.438AsnAsn: 0.438 ± 0.017
1.285AsnPro: 1.285 ± 0.024
0.513AsnGln: 0.513 ± 0.016
1.222AsnArg: 1.222 ± 0.026
0.902AsnSer: 0.902 ± 0.02
1.118AsnThr: 1.118 ± 0.023
1.375AsnVal: 1.375 ± 0.024
0.283AsnTrp: 0.283 ± 0.011
0.395AsnTyr: 0.395 ± 0.012
0.0AsnXaa: 0.0 ± 0.0
Pro
8.867ProAla: 8.867 ± 0.085
0.332ProCys: 0.332 ± 0.014
4.49ProAsp: 4.49 ± 0.052
4.32ProGlu: 4.32 ± 0.045
1.554ProPhe: 1.554 ± 0.023
6.742ProGly: 6.742 ± 0.074
1.447ProHis: 1.447 ± 0.026
1.179ProIle: 1.179 ± 0.024
1.082ProLys: 1.082 ± 0.023
5.503ProLeu: 5.503 ± 0.05
0.964ProMet: 0.964 ± 0.019
0.843ProAsn: 0.843 ± 0.018
3.687ProPro: 3.687 ± 0.053
1.771ProGln: 1.771 ± 0.032
4.04ProArg: 4.04 ± 0.047
3.178ProSer: 3.178 ± 0.042
3.225ProThr: 3.225 ± 0.041
5.637ProVal: 5.637 ± 0.053
0.939ProTrp: 0.939 ± 0.02
1.473ProTyr: 1.473 ± 0.025
0.0ProXaa: 0.0 ± 0.0
Gln
3.672GlnAla: 3.672 ± 0.047
0.181GlnCys: 0.181 ± 0.008
1.425GlnAsp: 1.425 ± 0.023
1.431GlnGlu: 1.431 ± 0.025
0.654GlnPhe: 0.654 ± 0.018
2.313GlnGly: 2.313 ± 0.03
0.656GlnHis: 0.656 ± 0.015
1.024GlnIle: 1.024 ± 0.022
0.609GlnLys: 0.609 ± 0.018
2.899GlnLeu: 2.899 ± 0.041
0.465GlnMet: 0.465 ± 0.012
0.494GlnAsn: 0.494 ± 0.016
1.654GlnPro: 1.654 ± 0.036
1.191GlnGln: 1.191 ± 0.03
2.309GlnArg: 2.309 ± 0.036
1.261GlnSer: 1.261 ± 0.025
1.36GlnThr: 1.36 ± 0.026
2.36GlnVal: 2.36 ± 0.034
0.471GlnTrp: 0.471 ± 0.013
0.6GlnTyr: 0.6 ± 0.015
0.0GlnXaa: 0.0 ± 0.0
Arg
10.184ArgAla: 10.184 ± 0.083
0.608ArgCys: 0.608 ± 0.018
4.27ArgAsp: 4.27 ± 0.051
4.775ArgGlu: 4.775 ± 0.055
2.379ArgPhe: 2.379 ± 0.034
5.953ArgGly: 5.953 ± 0.054
2.205ArgHis: 2.205 ± 0.03
3.183ArgIle: 3.183 ± 0.036
1.558ArgLys: 1.558 ± 0.029
9.319ArgLeu: 9.319 ± 0.074
1.673ArgMet: 1.673 ± 0.027
1.266ArgAsn: 1.266 ± 0.022
5.117ArgPro: 5.117 ± 0.054
2.29ArgGln: 2.29 ± 0.027
7.962ArgArg: 7.962 ± 0.073
3.93ArgSer: 3.93 ± 0.043
5.528ArgThr: 5.528 ± 0.054
6.056ArgVal: 6.056 ± 0.053
1.408ArgTrp: 1.408 ± 0.024
1.781ArgTyr: 1.781 ± 0.027
0.0ArgXaa: 0.0 ± 0.0
Ser
6.745SerAla: 6.745 ± 0.056
0.43SerCys: 0.43 ± 0.014
2.635SerAsp: 2.635 ± 0.035
2.3SerGlu: 2.3 ± 0.034
1.518SerPhe: 1.518 ± 0.025
5.937SerGly: 5.937 ± 0.065
1.006SerHis: 1.006 ± 0.022
1.308SerIle: 1.308 ± 0.029
0.958SerLys: 0.958 ± 0.025
4.872SerLeu: 4.872 ± 0.042
0.995SerMet: 0.995 ± 0.02
0.841SerAsn: 0.841 ± 0.022
3.198SerPro: 3.198 ± 0.038
1.204SerGln: 1.204 ± 0.025
3.618SerArg: 3.618 ± 0.038
2.895SerSer: 2.895 ± 0.044
3.129SerThr: 3.129 ± 0.043
4.235SerVal: 4.235 ± 0.044
0.916SerTrp: 0.916 ± 0.019
1.253SerTyr: 1.253 ± 0.023
0.0SerXaa: 0.0 ± 0.0
Thr
9.371ThrAla: 9.371 ± 0.074
0.452ThrCys: 0.452 ± 0.015
3.754ThrAsp: 3.754 ± 0.041
3.233ThrGlu: 3.233 ± 0.042
1.644ThrPhe: 1.644 ± 0.027
7.004ThrGly: 7.004 ± 0.066
1.265ThrHis: 1.265 ± 0.023
1.613ThrIle: 1.613 ± 0.028
1.133ThrLys: 1.133 ± 0.024
5.937ThrLeu: 5.937 ± 0.049
0.898ThrMet: 0.898 ± 0.021
0.996ThrAsn: 0.996 ± 0.023
4.263ThrPro: 4.263 ± 0.051
1.327ThrGln: 1.327 ± 0.026
4.039ThrArg: 4.039 ± 0.042
3.172ThrSer: 3.172 ± 0.044
3.999ThrThr: 3.999 ± 0.049
6.398ThrVal: 6.398 ± 0.054
0.898ThrTrp: 0.898 ± 0.019
1.403ThrTyr: 1.403 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
10.775ValAla: 10.775 ± 0.074
0.781ValCys: 0.781 ± 0.021
5.09ValAsp: 5.09 ± 0.047
4.72ValGlu: 4.72 ± 0.049
2.454ValPhe: 2.454 ± 0.031
6.562ValGly: 6.562 ± 0.058
2.046ValHis: 2.046 ± 0.028
2.705ValIle: 2.705 ± 0.032
1.731ValLys: 1.731 ± 0.031
9.701ValLeu: 9.701 ± 0.079
1.377ValMet: 1.377 ± 0.021
1.673ValAsn: 1.673 ± 0.027
5.427ValPro: 5.427 ± 0.046
2.108ValGln: 2.108 ± 0.031
7.528ValArg: 7.528 ± 0.069
4.342ValSer: 4.342 ± 0.042
5.904ValThr: 5.904 ± 0.054
8.272ValVal: 8.272 ± 0.066
1.162ValTrp: 1.162 ± 0.023
1.655ValTyr: 1.655 ± 0.03
0.0ValXaa: 0.0 ± 0.0
Trp
1.667TrpAla: 1.667 ± 0.028
0.16TrpCys: 0.16 ± 0.008
0.843TrpAsp: 0.843 ± 0.019
0.72TrpGlu: 0.72 ± 0.017
0.499TrpPhe: 0.499 ± 0.014
1.095TrpGly: 1.095 ± 0.022
0.366TrpHis: 0.366 ± 0.012
0.537TrpIle: 0.537 ± 0.016
0.371TrpLys: 0.371 ± 0.013
1.764TrpLeu: 1.764 ± 0.031
0.265TrpMet: 0.265 ± 0.01
0.427TrpAsn: 0.427 ± 0.012
0.774TrpPro: 0.774 ± 0.018
0.613TrpGln: 0.613 ± 0.015
1.346TrpArg: 1.346 ± 0.019
0.95TrpSer: 0.95 ± 0.022
1.097TrpThr: 1.097 ± 0.024
0.986TrpVal: 0.986 ± 0.023
0.336TrpTrp: 0.336 ± 0.012
0.38TrpTyr: 0.38 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.831TyrAla: 2.831 ± 0.034
0.174TyrCys: 0.174 ± 0.009
1.607TyrAsp: 1.607 ± 0.029
1.232TyrGlu: 1.232 ± 0.025
0.633TyrPhe: 0.633 ± 0.018
2.373TyrGly: 2.373 ± 0.036
0.407TyrHis: 0.407 ± 0.011
0.461TyrIle: 0.461 ± 0.016
0.389TyrLys: 0.389 ± 0.014
2.138TyrLeu: 2.138 ± 0.031
0.234TyrMet: 0.234 ± 0.011
0.396TyrAsn: 0.396 ± 0.014
1.075TyrPro: 1.075 ± 0.022
0.592TyrGln: 0.592 ± 0.015
1.861TyrArg: 1.861 ± 0.03
0.939TyrSer: 0.939 ± 0.021
1.236TyrThr: 1.236 ± 0.021
1.72TyrVal: 1.72 ± 0.03
0.356TyrTrp: 0.356 ± 0.012
0.483TyrTyr: 0.483 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7596 proteins (2550604 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski