Blautia wexlerae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Blautia

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3715 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A174C6S4|A0A174C6S4_9FIRM Cobalamin-binding protein OS=Blautia wexlerae OX=418240 GN=metH_2 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 10.2NKK4 pKa = 10.44KK5 pKa = 10.03LMSILLVSAILGTTLGTTAVSAADD29 pKa = 3.79EE30 pKa = 4.55TSVSLWTWSPITRR43 pKa = 11.84TAEE46 pKa = 3.78KK47 pKa = 10.06MIDD50 pKa = 3.52AFEE53 pKa = 4.31KK54 pKa = 10.66ANPDD58 pKa = 2.84ITIDD62 pKa = 3.64YY63 pKa = 9.2TNYY66 pKa = 10.63NYY68 pKa = 10.64NPEE71 pKa = 4.0YY72 pKa = 10.71LQALSAASASDD83 pKa = 3.51NLADD87 pKa = 4.79IVGLQPGSLTQTYY100 pKa = 10.72SDD102 pKa = 4.02YY103 pKa = 11.44LIDD106 pKa = 4.42LSDD109 pKa = 3.6YY110 pKa = 11.6AKK112 pKa = 11.03AEE114 pKa = 4.12WGDD117 pKa = 3.78DD118 pKa = 3.16WTSVYY123 pKa = 11.46DD124 pKa = 4.95NVTQSQLQLGNKK136 pKa = 9.99DD137 pKa = 3.55GDD139 pKa = 3.95DD140 pKa = 3.34GHH142 pKa = 6.99YY143 pKa = 10.0ILPIEE148 pKa = 4.34TQDD151 pKa = 4.36IYY153 pKa = 11.87VEE155 pKa = 4.14YY156 pKa = 10.76NKK158 pKa = 9.78TLFEE162 pKa = 3.96QLGLKK167 pKa = 10.27VPTTYY172 pKa = 11.07DD173 pKa = 3.27EE174 pKa = 4.28LVEE177 pKa = 4.15VSKK180 pKa = 9.07TLRR183 pKa = 11.84DD184 pKa = 3.32NGYY187 pKa = 10.55APLFFGGADD196 pKa = 3.16GWQHH200 pKa = 5.97VNLLLMCTSQISDD213 pKa = 3.72TLFDD217 pKa = 3.5EE218 pKa = 4.86CQNGEE223 pKa = 4.25KK224 pKa = 10.31AWTCDD229 pKa = 3.1EE230 pKa = 4.51MKK232 pKa = 10.6QAMTNYY238 pKa = 10.04KK239 pKa = 10.25KK240 pKa = 10.92LFDD243 pKa = 5.32DD244 pKa = 5.77GVMQDD249 pKa = 4.09GSLSSTSYY257 pKa = 11.59SDD259 pKa = 3.15GTTLFLAGQAGMMLLGSWWAQEE281 pKa = 3.98YY282 pKa = 9.17TSEE285 pKa = 4.22DD286 pKa = 3.28VSDD289 pKa = 5.75AVANWDD295 pKa = 3.08YY296 pKa = 11.59DD297 pKa = 4.09YY298 pKa = 11.14FYY300 pKa = 11.36LPALEE305 pKa = 5.01EE306 pKa = 4.31GLSDD310 pKa = 3.46SKK312 pKa = 11.58AIGGVDD318 pKa = 3.98FGYY321 pKa = 11.07GITKK325 pKa = 10.14NCEE328 pKa = 3.88NPDD331 pKa = 4.2LAWKK335 pKa = 10.28ALVSFATGEE344 pKa = 4.26GAQEE348 pKa = 4.17IANDD352 pKa = 3.95MNNHH356 pKa = 6.32LSYY359 pKa = 11.23PNIEE363 pKa = 4.68PDD365 pKa = 3.04TSAMVEE371 pKa = 3.77RR372 pKa = 11.84DD373 pKa = 3.13GLQNVVDD380 pKa = 3.92EE381 pKa = 5.1FNRR384 pKa = 11.84SGKK387 pKa = 10.23DD388 pKa = 2.89IAAGLVNQRR397 pKa = 11.84IAEE400 pKa = 4.13PTIEE404 pKa = 4.11TAIQEE409 pKa = 4.18AMQGLIGGTYY419 pKa = 10.31SVDD422 pKa = 3.11EE423 pKa = 4.46ATQHH427 pKa = 6.12IQDD430 pKa = 4.38AQDD433 pKa = 3.15ALL435 pKa = 3.86

Molecular weight: 47.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A174DFB4|A0A174DFB4_9FIRM Dihydroorotase OS=Blautia wexlerae OX=418240 GN=pyrC PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.15GGRR28 pKa = 11.84KK29 pKa = 8.69VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.41GRR39 pKa = 11.84KK40 pKa = 8.72HH41 pKa = 6.34LSAA44 pKa = 5.8

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3715

0

3715

1165781

29

2994

313.8

35.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.211 ± 0.044

1.556 ± 0.018

5.773 ± 0.035

7.655 ± 0.045

4.017 ± 0.029

6.927 ± 0.042

1.769 ± 0.019

7.308 ± 0.036

7.226 ± 0.036

8.717 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.151 ± 0.023

4.466 ± 0.027

3.259 ± 0.022

3.399 ± 0.024

4.323 ± 0.036

5.775 ± 0.033

5.54 ± 0.035

6.756 ± 0.037

0.948 ± 0.013

4.222 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski