Streptococcus phage Javan599

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 42 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6ALS5|A0A4D6ALS5_9CAUD Plasmid stabilization system antitoxin protein OS=Streptococcus phage Javan599 OX=2548280 GN=Javan599_0021 PE=4 SV=1
MM1 pKa = 6.71WTDD4 pKa = 3.72GRR6 pKa = 11.84IDD8 pKa = 3.66YY9 pKa = 10.43QGQKK13 pKa = 9.41VDD15 pKa = 4.19YY16 pKa = 8.62IAKK19 pKa = 9.96VSPQPSEE26 pKa = 3.82VGIDD30 pKa = 3.55LGCVFKK36 pKa = 11.22LDD38 pKa = 3.42IEE40 pKa = 4.55VAEE43 pKa = 4.24EE44 pKa = 4.3TIVSYY49 pKa = 11.22DD50 pKa = 3.91RR51 pKa = 11.84GWEE54 pKa = 4.0IYY56 pKa = 10.22PEE58 pKa = 4.29TEE60 pKa = 3.67DD61 pKa = 4.61RR62 pKa = 11.84EE63 pKa = 4.63AILEE67 pKa = 4.12AVLLVLTVV75 pKa = 3.22

Molecular weight: 8.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6ANV6|A0A4D6ANV6_9CAUD Portal protein OS=Streptococcus phage Javan599 OX=2548280 GN=Javan599_0024 PE=4 SV=1
MM1 pKa = 7.81DD2 pKa = 5.37AKK4 pKa = 9.75STKK7 pKa = 10.19AKK9 pKa = 10.15GYY11 pKa = 9.42NGQWTKK17 pKa = 11.25ARR19 pKa = 11.84LRR21 pKa = 11.84YY22 pKa = 10.0LKK24 pKa = 9.81VHH26 pKa = 6.46PLCVQCKK33 pKa = 9.42SKK35 pKa = 10.8GRR37 pKa = 11.84LTKK40 pKa = 10.05ATVVDD45 pKa = 5.28HH46 pKa = 6.23ITPHH50 pKa = 6.97RR51 pKa = 11.84GDD53 pKa = 5.06QEE55 pKa = 5.01LFWNQSNWQALCKK68 pKa = 10.09SCHH71 pKa = 5.64DD72 pKa = 4.22RR73 pKa = 11.84KK74 pKa = 8.93TKK76 pKa = 8.13TTDD79 pKa = 2.58RR80 pKa = 11.84YY81 pKa = 10.6VEE83 pKa = 3.73YY84 pKa = 9.41TYY86 pKa = 11.18RR87 pKa = 11.84FF88 pKa = 3.55

Molecular weight: 10.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

42

0

42

11986

44

1503

285.4

32.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.95 ± 0.455

0.793 ± 0.151

5.865 ± 0.356

7.242 ± 0.439

3.629 ± 0.171

6.666 ± 0.28

1.902 ± 0.161

6.591 ± 0.4

7.083 ± 0.277

9.036 ± 0.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.253 ± 0.222

4.505 ± 0.208

3.012 ± 0.232

4.689 ± 0.26

4.806 ± 0.301

6.833 ± 0.506

6.808 ± 0.575

6.391 ± 0.231

1.418 ± 0.11

3.529 ± 0.322
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski