Massilia lurida

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Massilia

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5075 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A562R8D7|A0A562R8D7_9BURK Electron transfer flavoprotein alpha subunit OS=Massilia lurida OX=1036180 GN=IP91_02746 PE=3 SV=1
MM1 pKa = 7.48NLTKK5 pKa = 10.39FFRR8 pKa = 11.84TAIAISALFVGVAQTAKK25 pKa = 10.75ADD27 pKa = 3.42IVTWTGDD34 pKa = 3.42TTGGPTVDD42 pKa = 4.39LTPLGADD49 pKa = 3.8ASAVPYY55 pKa = 9.72DD56 pKa = 3.33AWSFTVDD63 pKa = 3.16QSGEE67 pKa = 4.09YY68 pKa = 9.78TFLLTAVYY76 pKa = 10.34NIDD79 pKa = 3.7SVIVLYY85 pKa = 10.82EE86 pKa = 3.83NSFDD90 pKa = 4.6PNDD93 pKa = 3.73AFSNSIAAGDD103 pKa = 4.38DD104 pKa = 3.57DD105 pKa = 5.4VSLNTSSTWASLTAGTHH122 pKa = 4.09YY123 pKa = 9.79TLLVAGFGDD132 pKa = 4.01TDD134 pKa = 3.57YY135 pKa = 11.44GAYY138 pKa = 9.99SVTIGGPGLITAVPEE153 pKa = 4.0PSTWLMLGLGLAAVGYY169 pKa = 4.95TARR172 pKa = 11.84RR173 pKa = 11.84KK174 pKa = 9.71SQLL177 pKa = 3.23

Molecular weight: 18.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A562QV29|A0A562QV29_9BURK WxcM-like protein OS=Massilia lurida OX=1036180 GN=IP91_05050 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.57RR14 pKa = 11.84THH16 pKa = 5.79GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84QVLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5075

0

5075

1795910

39

2893

353.9

38.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.227 ± 0.052

0.827 ± 0.01

5.571 ± 0.023

5.024 ± 0.03

3.49 ± 0.02

8.417 ± 0.033

2.217 ± 0.015

4.391 ± 0.023

3.231 ± 0.034

10.451 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.341 ± 0.015

2.916 ± 0.024

5.228 ± 0.026

3.859 ± 0.022

6.824 ± 0.033

5.121 ± 0.026

5.57 ± 0.028

7.432 ± 0.026

1.374 ± 0.012

2.49 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski