Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3088 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F9ULK3|F9ULK3_LACPL Putative PTS system EIIA component OS=Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) OX=220668 GN=srlR2 PE=4 SV=1
MM1 pKa = 7.55 IPAQAGLNEE10 pKa = 4.31 HH11 pKa = 5.58 WQQRR15 pKa = 11.84 NDD17 pKa = 3.27 SRR19 pKa = 11.84 DD20 pKa = 3.26 WVLDD24 pKa = 3.45 ADD26 pKa = 4.12 NYY28 pKa = 11.14 CYY30 pKa = 10.93 DD31 pKa = 3.53 GDD33 pKa = 4.23 EE34 pKa = 4.14 FDD36 pKa = 5.21 KK37 pKa = 11.53 AQLFQDD43 pKa = 4.36 YY44 pKa = 10.61 IDD46 pKa = 4.75 NNDD49 pKa = 3.22 FEE51 pKa = 4.76 QWATDD56 pKa = 3.5 MQADD60 pKa = 4.27 MLSAICIVTFGSTDD74 pKa = 3.26 VSVLYY79 pKa = 9.62 PDD81 pKa = 4.34 QGEE84 pKa = 4.19 EE85 pKa = 5.0 PNWQWLIDD93 pKa = 3.72 VFGQSRR99 pKa = 11.84 LWDD102 pKa = 3.8 EE103 pKa = 4.47 LLVHH107 pKa = 6.94 IDD109 pKa = 3.71 TDD111 pKa = 3.62 TMMTRR116 pKa = 11.84 LGYY119 pKa = 9.35 HH120 pKa = 5.61 WVSEE124 pKa = 4.31 GEE126 pKa = 4.16 EE127 pKa = 3.98 AA128 pKa = 5.12
Molecular weight: 14.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.709
IPC2_protein 3.656
IPC_protein 3.656
Toseland 3.427
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.821
Wikipedia 3.617
Rodwell 3.478
Grimsley 3.338
Solomon 3.643
Lehninger 3.605
Nozaki 3.783
DTASelect 4.037
Thurlkill 3.503
EMBOSS 3.617
Sillero 3.783
Patrickios 0.947
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.721
Protein with the highest isoelectric point:
>tr|F9ULW3|F9ULW3_LACPL Uncharacterized protein OS=Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) OX=220668 GN=lp_0734 PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.69 QPKK8 pKa = 8.69 KK9 pKa = 7.77 RR10 pKa = 11.84 HH11 pKa = 4.99 RR12 pKa = 11.84 QRR14 pKa = 11.84 VHH16 pKa = 6.07 GFRR19 pKa = 11.84 KK20 pKa = 10.03 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 KK29 pKa = 8.01 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.8 VLSAA44 pKa = 4.11
Molecular weight: 5.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3087
1
3088
934580
29
5289
302.6
33.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.224 ± 0.052
0.505 ± 0.011
5.662 ± 0.088
4.513 ± 0.043
3.964 ± 0.036
6.648 ± 0.047
2.377 ± 0.021
6.553 ± 0.042
5.154 ± 0.038
10.012 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.678 ± 0.025
4.404 ± 0.03
3.798 ± 0.029
5.252 ± 0.045
4.287 ± 0.036
5.792 ± 0.105
7.093 ± 0.069
7.39 ± 0.035
1.175 ± 0.019
3.518 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here