Pontibacter ummariensis
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5129 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A239IZU8|A0A239IZU8_9BACT Uncharacterized protein OS=Pontibacter ummariensis OX=1610492 GN=SAMN06296052_119100 PE=4 SV=1
MM1 pKa = 7.73 KK2 pKa = 10.62 LPGHH6 pKa = 6.4 FLLLLSLFLLSCNNDD21 pKa = 3.84 DD22 pKa = 6.28 DD23 pKa = 5.75 DD24 pKa = 4.49 VAPNDD29 pKa = 3.22 VPEE32 pKa = 4.4 AVKK35 pKa = 9.6 STLTASFSNAMNVEE49 pKa = 4.03 WEE51 pKa = 4.37 KK52 pKa = 11.53 VGDD55 pKa = 3.71 NYY57 pKa = 10.69 VAEE60 pKa = 4.6 FEE62 pKa = 4.7 VTGNEE67 pKa = 3.58 YY68 pKa = 10.75 DD69 pKa = 4.08 ALIDD73 pKa = 4.11 PSGQLLLYY81 pKa = 10.19 KK82 pKa = 10.29 YY83 pKa = 9.65 DD84 pKa = 3.56 IPEE87 pKa = 4.28 SEE89 pKa = 4.99 LPDD92 pKa = 3.6 AVSAAIHH99 pKa = 5.41 QNYY102 pKa = 10.1 SDD104 pKa = 3.85 GQIDD108 pKa = 3.73 DD109 pKa = 4.55 AEE111 pKa = 4.04 RR112 pKa = 11.84 LYY114 pKa = 10.9 QGNAEE119 pKa = 4.86 FYY121 pKa = 10.78 QVDD124 pKa = 4.74 LDD126 pKa = 3.96 NKK128 pKa = 9.06 GTGLHH133 pKa = 6.43 LVFSPDD139 pKa = 3.05 GQEE142 pKa = 4.01 QTEE145 pKa = 4.06 PAYY148 pKa = 9.85 WDD150 pKa = 3.31
Molecular weight: 16.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.732
IPC_protein 3.719
Toseland 3.503
ProMoST 3.846
Dawson 3.719
Bjellqvist 3.91
Wikipedia 3.656
Rodwell 3.554
Grimsley 3.414
Solomon 3.706
Lehninger 3.656
Nozaki 3.834
DTASelect 4.062
Thurlkill 3.567
EMBOSS 3.668
Sillero 3.846
Patrickios 0.693
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.78
Protein with the highest isoelectric point:
>tr|A0A239AY20|A0A239AY20_9BACT Protein translocase subunit SecE OS=Pontibacter ummariensis OX=1610492 GN=secE PE=3 SV=1
MM1 pKa = 7.57 KK2 pKa = 10.3 ASCCGKK8 pKa = 10.32 LPEE11 pKa = 4.43 KK12 pKa = 10.5 RR13 pKa = 11.84 IAITSYY19 pKa = 8.81 RR20 pKa = 11.84 DD21 pKa = 3.23 EE22 pKa = 4.44 YY23 pKa = 11.1 EE24 pKa = 3.78 RR25 pKa = 11.84 TITRR29 pKa = 11.84 TRR31 pKa = 11.84 SRR33 pKa = 11.84 WGRR36 pKa = 11.84 RR37 pKa = 11.84 MKK39 pKa = 10.16 PLRR42 pKa = 11.84 QATVEE47 pKa = 4.11 LVLGTLLNHH56 pKa = 6.11 MGMRR60 pKa = 11.84 RR61 pKa = 11.84 INTRR65 pKa = 11.84 GLEE68 pKa = 4.02 LAHH71 pKa = 7.2 KK72 pKa = 10.02 GMLVAAAAYY81 pKa = 9.21 NLQKK85 pKa = 10.72 LLHH88 pKa = 5.11 FTPRR92 pKa = 11.84 RR93 pKa = 11.84 SQTAVMALPRR103 pKa = 11.84 PEE105 pKa = 3.87 QAVFFYY111 pKa = 9.99 FYY113 pKa = 10.13 FWPDD117 pKa = 3.04 QEE119 pKa = 4.65 VVVEE123 pKa = 3.92 VGIEE127 pKa = 3.82 VRR129 pKa = 11.84 RR130 pKa = 3.71
Molecular weight: 15.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.765
IPC_protein 10.687
Toseland 10.657
ProMoST 10.672
Dawson 10.774
Bjellqvist 10.526
Wikipedia 11.023
Rodwell 10.906
Grimsley 10.833
Solomon 10.906
Lehninger 10.862
Nozaki 10.643
DTASelect 10.526
Thurlkill 10.672
EMBOSS 11.067
Sillero 10.716
Patrickios 10.613
IPC_peptide 10.906
IPC2_peptide 9.633
IPC2.peptide.svr19 8.571
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5129
0
5129
1702560
24
2791
331.9
37.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.17 ± 0.041
0.743 ± 0.011
5.1 ± 0.025
6.782 ± 0.033
4.495 ± 0.024
7.062 ± 0.034
2.009 ± 0.018
5.604 ± 0.032
5.918 ± 0.035
10.279 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.281 ± 0.017
4.53 ± 0.031
4.185 ± 0.019
4.283 ± 0.026
4.76 ± 0.024
5.922 ± 0.028
5.502 ± 0.03
7.151 ± 0.028
1.203 ± 0.016
4.021 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here