Mycobacterium phage Amohnition

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A222ZNI9|A0A222ZNI9_9CAUD Membrane protein OS=Mycobacterium phage Amohnition OX=2015874 GN=44 PE=4 SV=1
MM1 pKa = 7.85RR2 pKa = 11.84APSTVEE8 pKa = 4.21VPCPACGEE16 pKa = 4.36PIEE19 pKa = 4.79LSLGFEE25 pKa = 4.42SVEE28 pKa = 3.92PAVEE32 pKa = 4.05GLNVASVVITTLDD45 pKa = 3.36LQDD48 pKa = 3.75RR49 pKa = 11.84VQSHH53 pKa = 6.39GEE55 pKa = 4.0VCPVLAGGGSDD66 pKa = 3.46GG67 pKa = 4.52

Molecular weight: 6.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A222ZPG8|A0A222ZPG8_9CAUD Portal protein OS=Mycobacterium phage Amohnition OX=2015874 GN=8 PE=4 SV=1
MM1 pKa = 6.98MRR3 pKa = 11.84CADD6 pKa = 4.53RR7 pKa = 11.84AWLALGAGVVAYY19 pKa = 8.94EE20 pKa = 3.81IAAPKK25 pKa = 10.82GEE27 pKa = 4.26LLSEE31 pKa = 4.34GVDD34 pKa = 2.97RR35 pKa = 11.84YY36 pKa = 11.07LNRR39 pKa = 11.84RR40 pKa = 11.84KK41 pKa = 7.82WTTRR45 pKa = 11.84VVVVGLAAHH54 pKa = 7.46LLNLIPQRR62 pKa = 11.84FDD64 pKa = 3.23PLTRR68 pKa = 11.84LAQATRR74 pKa = 11.84GSS76 pKa = 3.56

Molecular weight: 8.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

19040

40

1396

202.6

21.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.004 ± 0.477

1.213 ± 0.173

6.812 ± 0.275

5.662 ± 0.352

2.568 ± 0.155

8.839 ± 0.553

2.159 ± 0.221

3.603 ± 0.204

3.592 ± 0.177

8.477 ± 0.263

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.174 ± 0.102

2.684 ± 0.211

5.53 ± 0.268

3.157 ± 0.168

7.122 ± 0.393

5.11 ± 0.217

5.809 ± 0.197

8.167 ± 0.28

2.043 ± 0.112

2.274 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski