Amino acid dipepetide frequency for Mycobacterium phage Amohnition

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.745AlaAla: 21.745 ± 1.762
1.103AlaCys: 1.103 ± 0.273
9.664AlaAsp: 9.664 ± 0.761
8.719AlaGlu: 8.719 ± 0.807
3.046AlaPhe: 3.046 ± 0.47
8.877AlaGly: 8.877 ± 0.947
2.469AlaHis: 2.469 ± 0.399
5.095AlaIle: 5.095 ± 0.55
4.254AlaLys: 4.254 ± 0.449
13.078AlaLeu: 13.078 ± 0.844
3.309AlaMet: 3.309 ± 0.453
3.204AlaAsn: 3.204 ± 0.52
6.723AlaPro: 6.723 ± 0.581
4.57AlaGln: 4.57 ± 0.692
8.246AlaArg: 8.246 ± 0.665
4.78AlaSer: 4.78 ± 0.691
6.093AlaThr: 6.093 ± 0.622
10.4AlaVal: 10.4 ± 0.997
2.048AlaTrp: 2.048 ± 0.307
2.626AlaTyr: 2.626 ± 0.475
0.0AlaXaa: 0.0 ± 0.0
Cys
1.103CysAla: 1.103 ± 0.277
0.158CysCys: 0.158 ± 0.098
0.63CysAsp: 0.63 ± 0.218
0.998CysGlu: 0.998 ± 0.279
0.21CysPhe: 0.21 ± 0.11
1.681CysGly: 1.681 ± 0.392
0.315CysHis: 0.315 ± 0.157
0.315CysIle: 0.315 ± 0.119
0.578CysLys: 0.578 ± 0.159
0.945CysLeu: 0.945 ± 0.22
0.158CysMet: 0.158 ± 0.082
0.473CysAsn: 0.473 ± 0.142
0.735CysPro: 0.735 ± 0.245
0.315CysGln: 0.315 ± 0.112
0.63CysArg: 0.63 ± 0.158
1.05CysSer: 1.05 ± 0.292
0.683CysThr: 0.683 ± 0.207
0.578CysVal: 0.578 ± 0.219
0.525CysTrp: 0.525 ± 0.148
0.053CysTyr: 0.053 ± 0.045
0.0CysXaa: 0.0 ± 0.0
Asp
8.824AspAla: 8.824 ± 0.664
0.998AspCys: 0.998 ± 0.29
6.513AspAsp: 6.513 ± 0.762
6.04AspGlu: 6.04 ± 0.678
1.208AspPhe: 1.208 ± 0.271
6.46AspGly: 6.46 ± 0.475
1.261AspHis: 1.261 ± 0.278
1.156AspIle: 1.156 ± 0.224
2.153AspLys: 2.153 ± 0.44
5.305AspLeu: 5.305 ± 0.62
1.838AspMet: 1.838 ± 0.329
1.681AspAsn: 1.681 ± 0.254
4.149AspPro: 4.149 ± 0.449
1.786AspGln: 1.786 ± 0.318
5.357AspArg: 5.357 ± 0.614
3.362AspSer: 3.362 ± 0.411
2.994AspThr: 2.994 ± 0.386
4.675AspVal: 4.675 ± 0.465
0.998AspTrp: 0.998 ± 0.223
1.366AspTyr: 1.366 ± 0.297
0.0AspXaa: 0.0 ± 0.0
Glu
9.087GluAla: 9.087 ± 0.987
0.788GluCys: 0.788 ± 0.215
3.151GluAsp: 3.151 ± 0.413
1.156GluGlu: 1.156 ± 0.277
1.313GluPhe: 1.313 ± 0.261
3.677GluGly: 3.677 ± 0.443
1.786GluHis: 1.786 ± 0.367
1.786GluIle: 1.786 ± 0.346
1.733GluLys: 1.733 ± 0.293
4.99GluLeu: 4.99 ± 0.468
1.208GluMet: 1.208 ± 0.245
1.208GluAsn: 1.208 ± 0.262
3.362GluPro: 3.362 ± 0.567
2.889GluGln: 2.889 ± 0.313
5.305GluArg: 5.305 ± 0.776
2.364GluSer: 2.364 ± 0.373
1.996GluThr: 1.996 ± 0.266
5.988GluVal: 5.988 ± 0.758
0.998GluTrp: 0.998 ± 0.211
1.838GluTyr: 1.838 ± 0.386
0.0GluXaa: 0.0 ± 0.0
Phe
2.731PheAla: 2.731 ± 0.418
0.315PheCys: 0.315 ± 0.119
3.204PheAsp: 3.204 ± 0.344
0.893PheGlu: 0.893 ± 0.232
0.63PhePhe: 0.63 ± 0.175
2.784PheGly: 2.784 ± 0.441
0.158PheHis: 0.158 ± 0.082
0.84PheIle: 0.84 ± 0.211
1.156PheLys: 1.156 ± 0.25
1.943PheLeu: 1.943 ± 0.332
0.63PheMet: 0.63 ± 0.163
0.788PheAsn: 0.788 ± 0.207
1.471PhePro: 1.471 ± 0.274
0.63PheGln: 0.63 ± 0.156
1.576PheArg: 1.576 ± 0.328
0.893PheSer: 0.893 ± 0.193
1.786PheThr: 1.786 ± 0.308
2.311PheVal: 2.311 ± 0.291
0.473PheTrp: 0.473 ± 0.157
0.473PheTyr: 0.473 ± 0.14
0.0PheXaa: 0.0 ± 0.0
Gly
9.769GlyAla: 9.769 ± 0.98
1.471GlyCys: 1.471 ± 0.318
5.62GlyAsp: 5.62 ± 0.474
4.937GlyGlu: 4.937 ± 0.519
2.311GlyPhe: 2.311 ± 0.364
9.507GlyGly: 9.507 ± 1.544
1.418GlyHis: 1.418 ± 0.302
2.574GlyIle: 2.574 ± 0.5
4.465GlyLys: 4.465 ± 0.482
7.143GlyLeu: 7.143 ± 0.653
1.523GlyMet: 1.523 ± 0.302
3.046GlyAsn: 3.046 ± 0.468
3.624GlyPro: 3.624 ± 0.582
2.521GlyGln: 2.521 ± 0.471
5.305GlyArg: 5.305 ± 0.614
5.673GlySer: 5.673 ± 0.578
5.568GlyThr: 5.568 ± 0.468
7.353GlyVal: 7.353 ± 0.43
2.311GlyTrp: 2.311 ± 0.316
2.259GlyTyr: 2.259 ± 0.399
0.0GlyXaa: 0.0 ± 0.0
His
1.838HisAla: 1.838 ± 0.363
0.315HisCys: 0.315 ± 0.128
1.471HisAsp: 1.471 ± 0.342
1.05HisGlu: 1.05 ± 0.269
0.788HisPhe: 0.788 ± 0.185
2.259HisGly: 2.259 ± 0.398
0.473HisHis: 0.473 ± 0.154
0.735HisIle: 0.735 ± 0.2
0.368HisLys: 0.368 ± 0.131
1.943HisLeu: 1.943 ± 0.291
0.315HisMet: 0.315 ± 0.119
0.735HisAsn: 0.735 ± 0.176
1.156HisPro: 1.156 ± 0.261
0.525HisGln: 0.525 ± 0.157
1.681HisArg: 1.681 ± 0.284
0.84HisSer: 0.84 ± 0.225
1.313HisThr: 1.313 ± 0.282
2.574HisVal: 2.574 ± 0.398
0.368HisTrp: 0.368 ± 0.146
0.84HisTyr: 0.84 ± 0.216
0.0HisXaa: 0.0 ± 0.0
Ile
5.305IleAla: 5.305 ± 0.456
0.21IleCys: 0.21 ± 0.11
3.256IleAsp: 3.256 ± 0.416
3.309IleGlu: 3.309 ± 0.502
0.893IlePhe: 0.893 ± 0.216
3.782IleGly: 3.782 ± 0.856
0.315IleHis: 0.315 ± 0.147
0.84IleIle: 0.84 ± 0.174
1.313IleLys: 1.313 ± 0.214
2.626IleLeu: 2.626 ± 0.365
0.315IleMet: 0.315 ± 0.146
1.05IleAsn: 1.05 ± 0.221
1.523IlePro: 1.523 ± 0.35
0.63IleGln: 0.63 ± 0.184
2.574IleArg: 2.574 ± 0.344
1.786IleSer: 1.786 ± 0.215
2.364IleThr: 2.364 ± 0.324
2.784IleVal: 2.784 ± 0.354
0.683IleTrp: 0.683 ± 0.228
0.473IleTyr: 0.473 ± 0.168
0.0IleXaa: 0.0 ± 0.0
Lys
4.99LysAla: 4.99 ± 0.641
0.683LysCys: 0.683 ± 0.19
1.261LysAsp: 1.261 ± 0.288
0.63LysGlu: 0.63 ± 0.165
0.945LysPhe: 0.945 ± 0.212
3.414LysGly: 3.414 ± 0.429
0.998LysHis: 0.998 ± 0.242
1.576LysIle: 1.576 ± 0.363
0.683LysLys: 0.683 ± 0.157
4.097LysLeu: 4.097 ± 0.475
0.945LysMet: 0.945 ± 0.189
0.578LysAsn: 0.578 ± 0.216
2.836LysPro: 2.836 ± 0.418
1.103LysGln: 1.103 ± 0.23
2.731LysArg: 2.731 ± 0.457
1.523LysSer: 1.523 ± 0.253
1.786LysThr: 1.786 ± 0.331
3.414LysVal: 3.414 ± 0.465
0.683LysTrp: 0.683 ± 0.16
1.05LysTyr: 1.05 ± 0.28
0.0LysXaa: 0.0 ± 0.0
Leu
12.448LeuAla: 12.448 ± 0.812
0.63LeuCys: 0.63 ± 0.182
6.986LeuAsp: 6.986 ± 0.52
2.626LeuGlu: 2.626 ± 0.406
1.996LeuPhe: 1.996 ± 0.324
7.038LeuGly: 7.038 ± 0.482
1.576LeuHis: 1.576 ± 0.304
3.624LeuIle: 3.624 ± 0.482
2.889LeuLys: 2.889 ± 0.471
6.513LeuLeu: 6.513 ± 0.643
1.943LeuMet: 1.943 ± 0.354
2.469LeuAsn: 2.469 ± 0.353
4.622LeuPro: 4.622 ± 0.585
2.574LeuGln: 2.574 ± 0.325
6.618LeuArg: 6.618 ± 0.607
5.357LeuSer: 5.357 ± 0.44
4.99LeuThr: 4.99 ± 0.521
6.565LeuVal: 6.565 ± 0.662
1.681LeuTrp: 1.681 ± 0.381
1.576LeuTyr: 1.576 ± 0.326
0.0LeuXaa: 0.0 ± 0.0
Met
2.574MetAla: 2.574 ± 0.384
0.158MetCys: 0.158 ± 0.097
1.156MetAsp: 1.156 ± 0.263
0.525MetGlu: 0.525 ± 0.154
0.788MetPhe: 0.788 ± 0.209
1.628MetGly: 1.628 ± 0.3
0.473MetHis: 0.473 ± 0.193
0.788MetIle: 0.788 ± 0.178
0.263MetLys: 0.263 ± 0.106
1.471MetLeu: 1.471 ± 0.273
0.63MetMet: 0.63 ± 0.19
0.735MetAsn: 0.735 ± 0.243
1.103MetPro: 1.103 ± 0.261
0.735MetGln: 0.735 ± 0.193
1.733MetArg: 1.733 ± 0.283
2.469MetSer: 2.469 ± 0.289
1.733MetThr: 1.733 ± 0.287
1.786MetVal: 1.786 ± 0.271
0.42MetTrp: 0.42 ± 0.125
0.578MetTyr: 0.578 ± 0.189
0.0MetXaa: 0.0 ± 0.0
Asn
4.149AsnAla: 4.149 ± 0.69
0.473AsnCys: 0.473 ± 0.134
1.261AsnAsp: 1.261 ± 0.286
1.261AsnGlu: 1.261 ± 0.24
0.368AsnPhe: 0.368 ± 0.119
3.572AsnGly: 3.572 ± 0.743
0.368AsnHis: 0.368 ± 0.112
0.735AsnIle: 0.735 ± 0.223
1.103AsnLys: 1.103 ± 0.19
2.153AsnLeu: 2.153 ± 0.398
0.525AsnMet: 0.525 ± 0.165
0.683AsnAsn: 0.683 ± 0.259
2.206AsnPro: 2.206 ± 0.324
0.473AsnGln: 0.473 ± 0.148
1.628AsnArg: 1.628 ± 0.303
1.05AsnSer: 1.05 ± 0.251
1.786AsnThr: 1.786 ± 0.303
1.996AsnVal: 1.996 ± 0.337
0.42AsnTrp: 0.42 ± 0.153
0.63AsnTyr: 0.63 ± 0.186
0.0AsnXaa: 0.0 ± 0.0
Pro
7.248ProAla: 7.248 ± 0.662
0.473ProCys: 0.473 ± 0.175
3.782ProAsp: 3.782 ± 0.395
4.202ProGlu: 4.202 ± 0.578
1.523ProPhe: 1.523 ± 0.32
5.41ProGly: 5.41 ± 0.439
1.471ProHis: 1.471 ± 0.332
1.996ProIle: 1.996 ± 0.269
2.206ProLys: 2.206 ± 0.306
4.097ProLeu: 4.097 ± 0.459
1.208ProMet: 1.208 ± 0.229
1.261ProAsn: 1.261 ± 0.252
2.941ProPro: 2.941 ± 0.453
1.418ProGln: 1.418 ± 0.306
3.256ProArg: 3.256 ± 0.527
3.099ProSer: 3.099 ± 0.437
3.519ProThr: 3.519 ± 0.526
3.992ProVal: 3.992 ± 0.592
0.84ProTrp: 0.84 ± 0.204
1.366ProTyr: 1.366 ± 0.298
0.0ProXaa: 0.0 ± 0.0
Gln
4.254GlnAla: 4.254 ± 0.559
0.525GlnCys: 0.525 ± 0.219
1.05GlnAsp: 1.05 ± 0.204
0.998GlnGlu: 0.998 ± 0.186
0.63GlnPhe: 0.63 ± 0.162
2.679GlnGly: 2.679 ± 0.382
0.945GlnHis: 0.945 ± 0.199
1.996GlnIle: 1.996 ± 0.292
0.893GlnLys: 0.893 ± 0.226
2.311GlnLeu: 2.311 ± 0.38
0.788GlnMet: 0.788 ± 0.187
0.683GlnAsn: 0.683 ± 0.181
1.943GlnPro: 1.943 ± 0.29
1.471GlnGln: 1.471 ± 0.32
2.941GlnArg: 2.941 ± 0.426
1.418GlnSer: 1.418 ± 0.267
1.891GlnThr: 1.891 ± 0.303
2.731GlnVal: 2.731 ± 0.378
0.788GlnTrp: 0.788 ± 0.174
0.63GlnTyr: 0.63 ± 0.186
0.0GlnXaa: 0.0 ± 0.0
Arg
7.196ArgAla: 7.196 ± 0.892
1.05ArgCys: 1.05 ± 0.258
3.887ArgAsp: 3.887 ± 0.442
4.675ArgGlu: 4.675 ± 0.56
1.996ArgPhe: 1.996 ± 0.324
5.252ArgGly: 5.252 ± 0.542
1.628ArgHis: 1.628 ± 0.353
2.941ArgIle: 2.941 ± 0.384
3.309ArgLys: 3.309 ± 0.409
7.091ArgLeu: 7.091 ± 0.709
1.418ArgMet: 1.418 ± 0.326
2.521ArgAsn: 2.521 ± 0.403
3.467ArgPro: 3.467 ± 0.469
2.626ArgGln: 2.626 ± 0.286
6.093ArgArg: 6.093 ± 0.74
3.046ArgSer: 3.046 ± 0.33
3.939ArgThr: 3.939 ± 0.593
5.462ArgVal: 5.462 ± 0.53
2.048ArgTrp: 2.048 ± 0.347
1.576ArgTyr: 1.576 ± 0.324
0.0ArgXaa: 0.0 ± 0.0
Ser
6.198SerAla: 6.198 ± 0.637
0.263SerCys: 0.263 ± 0.119
2.469SerAsp: 2.469 ± 0.363
3.256SerGlu: 3.256 ± 0.417
1.418SerPhe: 1.418 ± 0.227
4.675SerGly: 4.675 ± 0.72
1.261SerHis: 1.261 ± 0.302
2.469SerIle: 2.469 ± 0.463
1.786SerLys: 1.786 ± 0.296
4.307SerLeu: 4.307 ± 0.358
1.523SerMet: 1.523 ± 0.267
1.156SerAsn: 1.156 ± 0.195
2.731SerPro: 2.731 ± 0.366
1.261SerGln: 1.261 ± 0.185
2.889SerArg: 2.889 ± 0.436
3.204SerSer: 3.204 ± 0.5
3.729SerThr: 3.729 ± 0.553
4.254SerVal: 4.254 ± 0.377
1.156SerTrp: 1.156 ± 0.289
1.103SerTyr: 1.103 ± 0.241
0.0SerXaa: 0.0 ± 0.0
Thr
7.091ThrAla: 7.091 ± 0.976
0.893ThrCys: 0.893 ± 0.246
2.941ThrAsp: 2.941 ± 0.477
3.151ThrGlu: 3.151 ± 0.411
2.311ThrPhe: 2.311 ± 0.285
5.778ThrGly: 5.778 ± 0.665
1.576ThrHis: 1.576 ± 0.339
2.679ThrIle: 2.679 ± 0.369
2.574ThrLys: 2.574 ± 0.338
3.677ThrLeu: 3.677 ± 0.494
0.945ThrMet: 0.945 ± 0.24
1.156ThrAsn: 1.156 ± 0.228
4.044ThrPro: 4.044 ± 0.45
1.523ThrGln: 1.523 ± 0.273
3.414ThrArg: 3.414 ± 0.509
2.626ThrSer: 2.626 ± 0.379
3.992ThrThr: 3.992 ± 0.636
4.412ThrVal: 4.412 ± 0.439
1.418ThrTrp: 1.418 ± 0.234
1.891ThrTyr: 1.891 ± 0.314
0.0ThrXaa: 0.0 ± 0.0
Val
9.349ValAla: 9.349 ± 0.929
1.05ValCys: 1.05 ± 0.216
7.301ValAsp: 7.301 ± 0.694
6.46ValGlu: 6.46 ± 0.659
2.206ValPhe: 2.206 ± 0.422
6.198ValGly: 6.198 ± 0.67
1.786ValHis: 1.786 ± 0.365
2.364ValIle: 2.364 ± 0.384
3.151ValLys: 3.151 ± 0.391
6.04ValLeu: 6.04 ± 0.539
1.366ValMet: 1.366 ± 0.212
2.048ValAsn: 2.048 ± 0.328
4.727ValPro: 4.727 ± 0.419
2.784ValGln: 2.784 ± 0.381
5.147ValArg: 5.147 ± 0.806
4.044ValSer: 4.044 ± 0.458
5.147ValThr: 5.147 ± 0.432
7.458ValVal: 7.458 ± 0.686
1.681ValTrp: 1.681 ± 0.299
1.366ValTyr: 1.366 ± 0.254
0.0ValXaa: 0.0 ± 0.0
Trp
1.681TrpAla: 1.681 ± 0.338
0.368TrpCys: 0.368 ± 0.122
1.156TrpAsp: 1.156 ± 0.319
0.525TrpGlu: 0.525 ± 0.143
0.683TrpPhe: 0.683 ± 0.175
1.313TrpGly: 1.313 ± 0.281
0.84TrpHis: 0.84 ± 0.222
0.893TrpIle: 0.893 ± 0.193
0.42TrpLys: 0.42 ± 0.104
2.889TrpLeu: 2.889 ± 0.403
0.42TrpMet: 0.42 ± 0.145
0.578TrpAsn: 0.578 ± 0.176
0.998TrpPro: 0.998 ± 0.216
0.945TrpGln: 0.945 ± 0.209
2.416TrpArg: 2.416 ± 0.367
1.208TrpSer: 1.208 ± 0.254
1.208TrpThr: 1.208 ± 0.228
1.05TrpVal: 1.05 ± 0.22
0.42TrpTrp: 0.42 ± 0.167
0.42TrpTyr: 0.42 ± 0.141
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.469TyrAla: 2.469 ± 0.317
0.21TyrCys: 0.21 ± 0.101
1.366TyrAsp: 1.366 ± 0.271
1.208TyrGlu: 1.208 ± 0.239
0.42TyrPhe: 0.42 ± 0.144
2.416TyrGly: 2.416 ± 0.34
0.473TyrHis: 0.473 ± 0.179
0.63TyrIle: 0.63 ± 0.197
0.63TyrLys: 0.63 ± 0.173
2.153TyrLeu: 2.153 ± 0.377
0.683TyrMet: 0.683 ± 0.19
0.788TyrAsn: 0.788 ± 0.224
0.945TyrPro: 0.945 ± 0.202
0.788TyrGln: 0.788 ± 0.262
1.681TyrArg: 1.681 ± 0.33
1.313TyrSer: 1.313 ± 0.259
1.576TyrThr: 1.576 ± 0.246
1.891TyrVal: 1.891 ± 0.29
0.473TyrTrp: 0.473 ± 0.191
0.63TyrTyr: 0.63 ± 0.174
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 94 proteins (19040 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski