Alteromonas phage JH01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4PYC9|A0A2Z4PYC9_9CAUD Uncharacterized protein OS=Alteromonas phage JH01 OX=2249314 PE=4 SV=1
MM1 pKa = 7.89PFLISGTITNNGNPVSVPVVAFSTTVPSILLGSTVSAADD40 pKa = 3.36GKK42 pKa = 8.86YY43 pKa = 9.03TISEE47 pKa = 4.26LEE49 pKa = 4.17YY50 pKa = 9.65KK51 pKa = 10.84GSAIVVAYY59 pKa = 10.84SNGGTLFNPTTVVLTDD75 pKa = 5.02DD76 pKa = 4.86IISPVPANGYY86 pKa = 8.18VYY88 pKa = 10.63RR89 pKa = 11.84ALNPGTLGNVEE100 pKa = 4.76PEE102 pKa = 3.8WATDD106 pKa = 3.49NNVISGDD113 pKa = 3.55VTLEE117 pKa = 4.39PIQVFAPSAAGYY129 pKa = 9.28EE130 pKa = 4.08NTLFYY135 pKa = 10.78MPDD138 pKa = 2.88SVQYY142 pKa = 10.39KK143 pKa = 9.41GRR145 pKa = 11.84NLVRR149 pKa = 11.84GTSAVVGGKK158 pKa = 7.9TYY160 pKa = 10.83YY161 pKa = 10.66AYY163 pKa = 11.06SDD165 pKa = 4.07TNDD168 pKa = 3.37PTYY171 pKa = 10.5IGYY174 pKa = 9.82FDD176 pKa = 3.91EE177 pKa = 5.71LIRR180 pKa = 11.84PLSITQLNVVNADD193 pKa = 3.7FEE195 pKa = 4.72TGDD198 pKa = 3.62LSNWQTTKK206 pKa = 9.59GTPDD210 pKa = 2.88VRR212 pKa = 11.84EE213 pKa = 3.83IARR216 pKa = 11.84GDD218 pKa = 3.46DD219 pKa = 3.42VRR221 pKa = 11.84NVVYY225 pKa = 10.27GAATLEE231 pKa = 4.31WGVSQQISLPILLSQIGYY249 pKa = 6.68PTDD252 pKa = 5.08AIPFLFDD259 pKa = 3.39NVTITLKK266 pKa = 10.54VAQSSYY272 pKa = 11.09NNSDD276 pKa = 3.37PAQQYY281 pKa = 9.94IRR283 pKa = 11.84LIDD286 pKa = 3.86SNNSEE291 pKa = 4.51ISFEE295 pKa = 4.51SNDD298 pKa = 3.67LKK300 pKa = 10.49TEE302 pKa = 4.14SSLEE306 pKa = 3.95WVEE309 pKa = 4.02RR310 pKa = 11.84TVSISANSNMDD321 pKa = 3.59LNAIEE326 pKa = 4.29VEE328 pKa = 4.25LYY330 pKa = 10.46GYY332 pKa = 7.65RR333 pKa = 11.84TNGSNNDD340 pKa = 3.36GYY342 pKa = 7.46TTSVRR347 pKa = 11.84VEE349 pKa = 4.19VVANEE354 pKa = 3.82

Molecular weight: 38.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4Q0C9|A0A2Z4Q0C9_9CAUD Uncharacterized protein OS=Alteromonas phage JH01 OX=2249314 PE=4 SV=1
MM1 pKa = 7.68AKK3 pKa = 9.13FTAEE7 pKa = 3.85QLGVLAEE14 pKa = 4.38KK15 pKa = 10.42IKK17 pKa = 11.29ANNTTINRR25 pKa = 11.84AASLAINKK33 pKa = 7.5TVTYY37 pKa = 10.39AKK39 pKa = 10.02DD40 pKa = 3.43LSISEE45 pKa = 3.95IVRR48 pKa = 11.84NVNLQQSYY56 pKa = 10.55IKK58 pKa = 10.62QNLKK62 pKa = 6.17TAKK65 pKa = 9.7RR66 pKa = 11.84ASPSDD71 pKa = 3.26LSAIIRR77 pKa = 11.84ANTRR81 pKa = 11.84EE82 pKa = 4.14TLLTRR87 pKa = 11.84YY88 pKa = 9.03PYY90 pKa = 10.47QEE92 pKa = 3.8TSTGVRR98 pKa = 11.84VAINKK103 pKa = 5.54TTGYY107 pKa = 8.72RR108 pKa = 11.84TINRR112 pKa = 11.84AFRR115 pKa = 11.84VTNLRR120 pKa = 11.84GSGATGIALKK130 pKa = 10.88NKK132 pKa = 9.91DD133 pKa = 3.26AVEE136 pKa = 4.02VFRR139 pKa = 11.84RR140 pKa = 11.84SLSPSTPGKK149 pKa = 9.54AAKK152 pKa = 9.71LARR155 pKa = 11.84IEE157 pKa = 4.6AKK159 pKa = 10.81AEE161 pKa = 4.08TKK163 pKa = 10.6PNGITVLHH171 pKa = 6.32SRR173 pKa = 11.84SINQLFTSVRR183 pKa = 11.84EE184 pKa = 4.08DD185 pKa = 3.43VQPRR189 pKa = 11.84TYY191 pKa = 11.03RR192 pKa = 11.84FMADD196 pKa = 3.13QFISDD201 pKa = 4.18LQRR204 pKa = 11.84LKK206 pKa = 11.18GKK208 pKa = 9.55SS209 pKa = 3.14

Molecular weight: 23.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

14468

39

1400

249.4

27.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.412 ± 0.66

1.092 ± 0.189

6.207 ± 0.277

6.919 ± 0.514

3.49 ± 0.24

6.359 ± 0.276

1.825 ± 0.286

5.391 ± 0.191

5.426 ± 0.387

8.301 ± 0.584

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.046 ± 0.229

5.391 ± 0.302

4.036 ± 0.482

4.147 ± 0.526

5.267 ± 0.322

6.186 ± 0.356

7.071 ± 0.357

7.7 ± 0.476

1.127 ± 0.14

3.608 ± 0.258
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski