Gordonia phage Gray

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Bendigovirus; unclassified Bendigovirus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 124 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S9UCB1|A0A3S9UCB1_9CAUD Uncharacterized protein OS=Gordonia phage Gray OX=2499024 GN=84 PE=4 SV=1
MM1 pKa = 7.63DD2 pKa = 5.26HH3 pKa = 7.16VDD5 pKa = 3.64EE6 pKa = 4.81SLQEE10 pKa = 5.0AMDD13 pKa = 3.43QYY15 pKa = 11.73RR16 pKa = 11.84NLLAEE21 pKa = 5.32CGLSDD26 pKa = 4.96CPYY29 pKa = 11.21NMTAFKK35 pKa = 9.77MAWFAKK41 pKa = 10.49DD42 pKa = 3.64LMASHH47 pKa = 7.12SDD49 pKa = 3.03MDD51 pKa = 3.89FMLRR55 pKa = 11.84VYY57 pKa = 10.08WSDD60 pKa = 4.28RR61 pKa = 11.84FEE63 pKa = 3.89LWLVYY68 pKa = 10.28ICIVTDD74 pKa = 3.56NDD76 pKa = 2.91EE77 pKa = 4.44CAPVLMVMTSEE88 pKa = 4.74YY89 pKa = 7.57ITKK92 pKa = 9.71VSRR95 pKa = 11.84MGQALSCGGEE105 pKa = 4.39GCSHH109 pKa = 6.39EE110 pKa = 4.51VCEE113 pKa = 5.06LVPDD117 pKa = 4.29SLRR120 pKa = 11.84DD121 pKa = 3.6PPRR124 pKa = 11.84SGLANADD131 pKa = 3.38PDD133 pKa = 4.78DD134 pKa = 4.12INGRR138 pKa = 11.84FSPIVEE144 pKa = 4.47SVEE147 pKa = 3.93GMPSFEE153 pKa = 4.4EE154 pKa = 3.79LAKK157 pKa = 10.64FYY159 pKa = 10.66DD160 pKa = 3.73VPEE163 pKa = 4.66DD164 pKa = 3.69KK165 pKa = 11.05EE166 pKa = 4.46GGG168 pKa = 3.63

Molecular weight: 18.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S9UCH9|A0A3S9UCH9_9CAUD YadA_stalk domain-containing protein OS=Gordonia phage Gray OX=2499024 GN=118 PE=4 SV=1
MM1 pKa = 7.36IRR3 pKa = 11.84NLRR6 pKa = 11.84NEE8 pKa = 3.75VRR10 pKa = 11.84PNEE13 pKa = 3.93LSTRR17 pKa = 11.84LWNMSKK23 pKa = 10.63GYY25 pKa = 9.98SRR27 pKa = 11.84WDD29 pKa = 3.32RR30 pKa = 11.84EE31 pKa = 3.75KK32 pKa = 10.92LKK34 pKa = 10.75RR35 pKa = 11.84VAVAMRR41 pKa = 11.84WEE43 pKa = 3.98IQNYY47 pKa = 9.74AEE49 pKa = 4.38TGRR52 pKa = 11.84VTDD55 pKa = 3.85GG56 pKa = 3.31

Molecular weight: 6.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

124

0

124

28213

46

1116

227.5

25.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.318 ± 0.275

0.826 ± 0.088

6.951 ± 0.284

6.196 ± 0.339

2.942 ± 0.137

8.096 ± 0.236

1.84 ± 0.108

4.828 ± 0.203

5.182 ± 0.245

7.181 ± 0.149

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.634 ± 0.162

4.161 ± 0.175

5.072 ± 0.198

3.793 ± 0.142

6.607 ± 0.218

5.827 ± 0.198

6.717 ± 0.288

7.256 ± 0.207

1.609 ± 0.13

2.967 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski