Amino acid dipepetide frequency for Gordonia phage Gray

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.606AlaAla: 9.606 ± 1.064
0.673AlaCys: 0.673 ± 0.168
5.849AlaAsp: 5.849 ± 0.526
5.459AlaGlu: 5.459 ± 0.466
2.977AlaPhe: 2.977 ± 0.437
7.798AlaGly: 7.798 ± 0.554
2.056AlaHis: 2.056 ± 0.368
4.821AlaIle: 4.821 ± 0.687
5.033AlaLys: 5.033 ± 0.606
7.408AlaLeu: 7.408 ± 0.623
2.836AlaMet: 2.836 ± 0.338
3.651AlaAsn: 3.651 ± 0.434
4.147AlaPro: 4.147 ± 0.42
3.934AlaGln: 3.934 ± 0.336
5.459AlaArg: 5.459 ± 0.455
5.565AlaSer: 5.565 ± 0.428
5.494AlaThr: 5.494 ± 0.574
6.77AlaVal: 6.77 ± 0.611
1.134AlaTrp: 1.134 ± 0.212
2.517AlaTyr: 2.517 ± 0.304
0.0AlaXaa: 0.0 ± 0.0
Cys
0.744CysAla: 0.744 ± 0.17
0.071CysCys: 0.071 ± 0.055
0.461CysAsp: 0.461 ± 0.128
0.425CysGlu: 0.425 ± 0.118
0.177CysPhe: 0.177 ± 0.087
0.922CysGly: 0.922 ± 0.214
0.213CysHis: 0.213 ± 0.091
0.354CysIle: 0.354 ± 0.126
0.39CysLys: 0.39 ± 0.112
0.39CysLeu: 0.39 ± 0.111
0.425CysMet: 0.425 ± 0.13
0.177CysAsn: 0.177 ± 0.075
0.673CysPro: 0.673 ± 0.158
0.496CysGln: 0.496 ± 0.141
0.284CysArg: 0.284 ± 0.108
0.567CysSer: 0.567 ± 0.167
0.461CysThr: 0.461 ± 0.138
0.603CysVal: 0.603 ± 0.152
0.142CysTrp: 0.142 ± 0.062
0.284CysTyr: 0.284 ± 0.084
0.0CysXaa: 0.0 ± 0.0
Asp
7.018AspAla: 7.018 ± 0.643
0.461AspCys: 0.461 ± 0.157
6.238AspAsp: 6.238 ± 0.954
5.849AspGlu: 5.849 ± 0.931
2.056AspPhe: 2.056 ± 0.267
5.14AspGly: 5.14 ± 0.414
1.382AspHis: 1.382 ± 0.233
3.119AspIle: 3.119 ± 0.326
2.836AspLys: 2.836 ± 0.407
4.927AspLeu: 4.927 ± 0.476
2.304AspMet: 2.304 ± 0.32
2.198AspAsn: 2.198 ± 0.262
4.147AspPro: 4.147 ± 0.396
2.127AspGln: 2.127 ± 0.354
4.36AspArg: 4.36 ± 0.352
3.651AspSer: 3.651 ± 0.419
3.722AspThr: 3.722 ± 0.395
4.785AspVal: 4.785 ± 0.331
1.099AspTrp: 1.099 ± 0.236
2.091AspTyr: 2.091 ± 0.269
0.0AspXaa: 0.0 ± 0.0
Glu
4.75GluAla: 4.75 ± 0.426
0.673GluCys: 0.673 ± 0.153
5.388GluAsp: 5.388 ± 1.017
4.537GluGlu: 4.537 ± 0.724
1.914GluPhe: 1.914 ± 0.278
4.36GluGly: 4.36 ± 0.387
1.311GluHis: 1.311 ± 0.219
2.8GluIle: 2.8 ± 0.281
2.623GluLys: 2.623 ± 0.411
5.388GluLeu: 5.388 ± 0.468
1.382GluMet: 1.382 ± 0.236
2.162GluAsn: 2.162 ± 0.295
2.41GluPro: 2.41 ± 0.308
3.013GluGln: 3.013 ± 0.398
3.793GluArg: 3.793 ± 0.447
3.048GluSer: 3.048 ± 0.345
3.686GluThr: 3.686 ± 0.371
4.821GluVal: 4.821 ± 0.402
1.418GluTrp: 1.418 ± 0.223
2.481GluTyr: 2.481 ± 0.344
0.0GluXaa: 0.0 ± 0.0
Phe
2.658PheAla: 2.658 ± 0.349
0.142PheCys: 0.142 ± 0.09
2.304PheAsp: 2.304 ± 0.303
2.091PheGlu: 2.091 ± 0.254
0.709PhePhe: 0.709 ± 0.137
2.481PheGly: 2.481 ± 0.319
0.496PheHis: 0.496 ± 0.131
1.453PheIle: 1.453 ± 0.201
1.347PheLys: 1.347 ± 0.266
1.808PheLeu: 1.808 ± 0.248
0.638PheMet: 0.638 ± 0.197
1.205PheAsn: 1.205 ± 0.23
1.382PhePro: 1.382 ± 0.25
1.311PheGln: 1.311 ± 0.164
1.843PheArg: 1.843 ± 0.308
2.233PheSer: 2.233 ± 0.291
1.631PheThr: 1.631 ± 0.247
2.552PheVal: 2.552 ± 0.336
0.532PheTrp: 0.532 ± 0.141
0.603PheTyr: 0.603 ± 0.146
0.0PheXaa: 0.0 ± 0.0
Gly
6.841GlyAla: 6.841 ± 0.559
0.496GlyCys: 0.496 ± 0.178
5.671GlyAsp: 5.671 ± 0.439
4.714GlyGlu: 4.714 ± 0.442
2.269GlyPhe: 2.269 ± 0.329
7.089GlyGly: 7.089 ± 0.742
1.347GlyHis: 1.347 ± 0.208
4.431GlyIle: 4.431 ± 0.441
3.615GlyLys: 3.615 ± 0.388
5.919GlyLeu: 5.919 ± 0.485
2.623GlyMet: 2.623 ± 0.432
3.261GlyAsn: 3.261 ± 0.305
2.517GlyPro: 2.517 ± 0.275
3.261GlyGln: 3.261 ± 0.36
5.246GlyArg: 5.246 ± 0.457
4.927GlySer: 4.927 ± 0.462
6.522GlyThr: 6.522 ± 0.538
6.026GlyVal: 6.026 ± 0.501
1.347GlyTrp: 1.347 ± 0.261
2.836GlyTyr: 2.836 ± 0.272
0.0GlyXaa: 0.0 ± 0.0
His
1.453HisAla: 1.453 ± 0.21
0.248HisCys: 0.248 ± 0.099
1.241HisAsp: 1.241 ± 0.238
1.241HisGlu: 1.241 ± 0.217
0.673HisPhe: 0.673 ± 0.152
1.311HisGly: 1.311 ± 0.222
0.567HisHis: 0.567 ± 0.154
0.815HisIle: 0.815 ± 0.176
0.744HisLys: 0.744 ± 0.177
1.134HisLeu: 1.134 ± 0.215
0.39HisMet: 0.39 ± 0.106
0.992HisAsn: 0.992 ± 0.226
1.134HisPro: 1.134 ± 0.2
0.425HisGln: 0.425 ± 0.111
1.063HisArg: 1.063 ± 0.225
0.886HisSer: 0.886 ± 0.184
1.843HisThr: 1.843 ± 0.358
1.453HisVal: 1.453 ± 0.24
0.354HisTrp: 0.354 ± 0.107
0.425HisTyr: 0.425 ± 0.12
0.0HisXaa: 0.0 ± 0.0
Ile
5.033IleAla: 5.033 ± 0.442
0.496IleCys: 0.496 ± 0.134
3.757IleAsp: 3.757 ± 0.271
3.119IleGlu: 3.119 ± 0.399
0.851IlePhe: 0.851 ± 0.172
4.041IleGly: 4.041 ± 0.46
0.673IleHis: 0.673 ± 0.157
2.375IleIle: 2.375 ± 0.415
2.8IleLys: 2.8 ± 0.399
3.048IleLeu: 3.048 ± 0.349
0.78IleMet: 0.78 ± 0.17
2.162IleAsn: 2.162 ± 0.245
2.658IlePro: 2.658 ± 0.283
1.56IleGln: 1.56 ± 0.213
2.907IleArg: 2.907 ± 0.312
3.367IleSer: 3.367 ± 0.373
3.686IleThr: 3.686 ± 0.434
3.474IleVal: 3.474 ± 0.496
0.638IleTrp: 0.638 ± 0.196
0.851IleTyr: 0.851 ± 0.157
0.0IleXaa: 0.0 ± 0.0
Lys
5.919LysAla: 5.919 ± 0.602
0.248LysCys: 0.248 ± 0.098
2.517LysAsp: 2.517 ± 0.28
2.765LysGlu: 2.765 ± 0.4
1.737LysPhe: 1.737 ± 0.239
3.899LysGly: 3.899 ± 0.372
0.603LysHis: 0.603 ± 0.147
1.701LysIle: 1.701 ± 0.233
4.36LysLys: 4.36 ± 0.807
3.828LysLeu: 3.828 ± 0.372
1.737LysMet: 1.737 ± 0.265
1.772LysAsn: 1.772 ± 0.281
3.226LysPro: 3.226 ± 0.368
1.95LysGln: 1.95 ± 0.305
4.537LysArg: 4.537 ± 0.547
3.261LysSer: 3.261 ± 0.402
2.517LysThr: 2.517 ± 0.313
3.048LysVal: 3.048 ± 0.343
0.461LysTrp: 0.461 ± 0.155
1.737LysTyr: 1.737 ± 0.255
0.0LysXaa: 0.0 ± 0.0
Leu
6.877LeuAla: 6.877 ± 0.603
0.532LeuCys: 0.532 ± 0.141
5.033LeuAsp: 5.033 ± 0.488
3.97LeuGlu: 3.97 ± 0.454
2.198LeuPhe: 2.198 ± 0.295
5.317LeuGly: 5.317 ± 0.491
0.922LeuHis: 0.922 ± 0.144
3.048LeuIle: 3.048 ± 0.351
3.226LeuLys: 3.226 ± 0.352
5.388LeuLeu: 5.388 ± 0.412
1.737LeuMet: 1.737 ± 0.27
3.438LeuAsn: 3.438 ± 0.326
4.147LeuPro: 4.147 ± 0.443
3.048LeuGln: 3.048 ± 0.385
5.14LeuArg: 5.14 ± 0.36
5.069LeuSer: 5.069 ± 0.464
4.927LeuThr: 4.927 ± 0.553
4.785LeuVal: 4.785 ± 0.406
0.957LeuTrp: 0.957 ± 0.178
2.056LeuTyr: 2.056 ± 0.233
0.0LeuXaa: 0.0 ± 0.0
Met
3.048MetAla: 3.048 ± 0.316
0.213MetCys: 0.213 ± 0.092
1.524MetAsp: 1.524 ± 0.279
1.701MetGlu: 1.701 ± 0.317
0.496MetPhe: 0.496 ± 0.123
1.772MetGly: 1.772 ± 0.315
0.532MetHis: 0.532 ± 0.157
1.099MetIle: 1.099 ± 0.225
1.489MetLys: 1.489 ± 0.293
1.879MetLeu: 1.879 ± 0.236
0.532MetMet: 0.532 ± 0.135
1.17MetAsn: 1.17 ± 0.226
1.701MetPro: 1.701 ± 0.242
0.957MetGln: 0.957 ± 0.177
1.56MetArg: 1.56 ± 0.209
1.808MetSer: 1.808 ± 0.283
2.304MetThr: 2.304 ± 0.269
1.666MetVal: 1.666 ± 0.252
0.354MetTrp: 0.354 ± 0.121
0.532MetTyr: 0.532 ± 0.137
0.0MetXaa: 0.0 ± 0.0
Asn
3.438AsnAla: 3.438 ± 0.438
0.461AsnCys: 0.461 ± 0.141
2.8AsnAsp: 2.8 ± 0.386
2.304AsnGlu: 2.304 ± 0.274
1.205AsnPhe: 1.205 ± 0.222
4.254AsnGly: 4.254 ± 0.513
0.567AsnHis: 0.567 ± 0.148
2.304AsnIle: 2.304 ± 0.253
1.453AsnLys: 1.453 ± 0.231
3.19AsnLeu: 3.19 ± 0.423
1.17AsnMet: 1.17 ± 0.244
1.666AsnAsn: 1.666 ± 0.351
2.198AsnPro: 2.198 ± 0.251
1.347AsnGln: 1.347 ± 0.221
2.481AsnArg: 2.481 ± 0.243
2.269AsnSer: 2.269 ± 0.333
3.332AsnThr: 3.332 ± 0.407
3.048AsnVal: 3.048 ± 0.329
0.425AsnTrp: 0.425 ± 0.143
1.701AsnTyr: 1.701 ± 0.263
0.0AsnXaa: 0.0 ± 0.0
Pro
4.431ProAla: 4.431 ± 0.425
0.425ProCys: 0.425 ± 0.115
4.324ProAsp: 4.324 ± 0.461
3.403ProGlu: 3.403 ± 0.317
1.17ProPhe: 1.17 ± 0.177
4.608ProGly: 4.608 ± 0.378
0.922ProHis: 0.922 ± 0.143
2.8ProIle: 2.8 ± 0.349
3.013ProLys: 3.013 ± 0.402
2.658ProLeu: 2.658 ± 0.345
1.063ProMet: 1.063 ± 0.198
2.02ProAsn: 2.02 ± 0.284
2.552ProPro: 2.552 ± 0.444
1.382ProGln: 1.382 ± 0.277
2.836ProArg: 2.836 ± 0.297
3.403ProSer: 3.403 ± 0.306
3.615ProThr: 3.615 ± 0.553
3.864ProVal: 3.864 ± 0.364
0.815ProTrp: 0.815 ± 0.151
1.418ProTyr: 1.418 ± 0.229
0.0ProXaa: 0.0 ± 0.0
Gln
3.97GlnAla: 3.97 ± 0.364
0.284GlnCys: 0.284 ± 0.085
1.631GlnAsp: 1.631 ± 0.284
2.127GlnGlu: 2.127 ± 0.286
1.241GlnPhe: 1.241 ± 0.187
2.375GlnGly: 2.375 ± 0.361
0.815GlnHis: 0.815 ± 0.164
2.198GlnIle: 2.198 ± 0.332
1.347GlnLys: 1.347 ± 0.236
3.509GlnLeu: 3.509 ± 0.305
1.134GlnMet: 1.134 ± 0.249
1.772GlnAsn: 1.772 ± 0.32
1.914GlnPro: 1.914 ± 0.268
1.879GlnGln: 1.879 ± 0.291
2.907GlnArg: 2.907 ± 0.334
1.701GlnSer: 1.701 ± 0.272
2.41GlnThr: 2.41 ± 0.266
2.41GlnVal: 2.41 ± 0.261
1.028GlnTrp: 1.028 ± 0.19
1.276GlnTyr: 1.276 ± 0.181
0.0GlnXaa: 0.0 ± 0.0
Arg
5.069ArgAla: 5.069 ± 0.365
0.886ArgCys: 0.886 ± 0.201
4.147ArgAsp: 4.147 ± 0.398
3.864ArgGlu: 3.864 ± 0.488
2.02ArgPhe: 2.02 ± 0.298
4.502ArgGly: 4.502 ± 0.413
1.382ArgHis: 1.382 ± 0.202
3.403ArgIle: 3.403 ± 0.369
4.962ArgLys: 4.962 ± 0.607
4.75ArgLeu: 4.75 ± 0.434
1.737ArgMet: 1.737 ± 0.247
2.552ArgAsn: 2.552 ± 0.283
2.942ArgPro: 2.942 ± 0.338
2.02ArgGln: 2.02 ± 0.273
5.317ArgArg: 5.317 ± 0.577
3.119ArgSer: 3.119 ± 0.362
4.36ArgThr: 4.36 ± 0.425
5.565ArgVal: 5.565 ± 0.453
1.276ArgTrp: 1.276 ± 0.244
2.162ArgTyr: 2.162 ± 0.264
0.0ArgXaa: 0.0 ± 0.0
Ser
5.388SerAla: 5.388 ± 0.462
0.319SerCys: 0.319 ± 0.126
4.36SerAsp: 4.36 ± 0.457
3.261SerGlu: 3.261 ± 0.405
2.091SerPhe: 2.091 ± 0.298
6.132SerGly: 6.132 ± 0.539
0.886SerHis: 0.886 ± 0.233
3.155SerIle: 3.155 ± 0.426
2.091SerLys: 2.091 ± 0.245
4.076SerLeu: 4.076 ± 0.457
1.205SerMet: 1.205 ± 0.211
2.339SerAsn: 2.339 ± 0.395
2.552SerPro: 2.552 ± 0.356
2.446SerGln: 2.446 ± 0.28
3.757SerArg: 3.757 ± 0.35
3.438SerSer: 3.438 ± 0.539
4.218SerThr: 4.218 ± 0.514
4.147SerVal: 4.147 ± 0.407
0.744SerTrp: 0.744 ± 0.181
1.666SerTyr: 1.666 ± 0.337
0.0SerXaa: 0.0 ± 0.0
Thr
6.522ThrAla: 6.522 ± 0.758
0.532ThrCys: 0.532 ± 0.167
4.147ThrAsp: 4.147 ± 0.489
4.041ThrGlu: 4.041 ± 0.395
1.737ThrPhe: 1.737 ± 0.245
5.636ThrGly: 5.636 ± 0.557
1.489ThrHis: 1.489 ± 0.204
3.19ThrIle: 3.19 ± 0.47
3.864ThrLys: 3.864 ± 0.331
4.041ThrLeu: 4.041 ± 0.452
1.276ThrMet: 1.276 ± 0.227
3.19ThrAsn: 3.19 ± 0.488
4.466ThrPro: 4.466 ± 0.415
2.269ThrGln: 2.269 ± 0.31
4.041ThrArg: 4.041 ± 0.401
3.757ThrSer: 3.757 ± 0.496
4.998ThrThr: 4.998 ± 0.594
4.998ThrVal: 4.998 ± 0.448
1.134ThrTrp: 1.134 ± 0.217
1.843ThrTyr: 1.843 ± 0.234
0.0ThrXaa: 0.0 ± 0.0
Val
6.628ValAla: 6.628 ± 0.499
0.638ValCys: 0.638 ± 0.162
4.714ValAsp: 4.714 ± 0.384
4.466ValGlu: 4.466 ± 0.402
2.588ValPhe: 2.588 ± 0.362
5.14ValGly: 5.14 ± 0.579
1.099ValHis: 1.099 ± 0.215
3.474ValIle: 3.474 ± 0.408
4.537ValLys: 4.537 ± 0.428
4.962ValLeu: 4.962 ± 0.47
2.091ValMet: 2.091 ± 0.409
3.403ValAsn: 3.403 ± 0.379
3.828ValPro: 3.828 ± 0.401
2.588ValGln: 2.588 ± 0.331
5.565ValArg: 5.565 ± 0.45
3.934ValSer: 3.934 ± 0.367
4.785ValThr: 4.785 ± 0.509
4.927ValVal: 4.927 ± 0.585
1.489ValTrp: 1.489 ± 0.246
1.701ValTyr: 1.701 ± 0.3
0.0ValXaa: 0.0 ± 0.0
Trp
1.17TrpAla: 1.17 ± 0.225
0.177TrpCys: 0.177 ± 0.087
1.347TrpAsp: 1.347 ± 0.252
1.028TrpGlu: 1.028 ± 0.171
0.567TrpPhe: 0.567 ± 0.167
0.922TrpGly: 0.922 ± 0.239
0.532TrpHis: 0.532 ± 0.14
0.673TrpIle: 0.673 ± 0.148
1.028TrpLys: 1.028 ± 0.275
1.099TrpLeu: 1.099 ± 0.192
0.461TrpMet: 0.461 ± 0.159
0.957TrpAsn: 0.957 ± 0.312
0.78TrpPro: 0.78 ± 0.156
0.461TrpGln: 0.461 ± 0.128
1.063TrpArg: 1.063 ± 0.196
0.815TrpSer: 0.815 ± 0.193
0.957TrpThr: 0.957 ± 0.181
1.382TrpVal: 1.382 ± 0.242
0.177TrpTrp: 0.177 ± 0.088
0.496TrpTyr: 0.496 ± 0.136
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.623TyrAla: 2.623 ± 0.319
0.284TyrCys: 0.284 ± 0.09
2.056TyrAsp: 2.056 ± 0.344
1.595TyrGlu: 1.595 ± 0.248
0.744TyrPhe: 0.744 ± 0.14
3.261TyrGly: 3.261 ± 0.447
0.603TyrHis: 0.603 ± 0.134
1.063TyrIle: 1.063 ± 0.209
1.063TyrLys: 1.063 ± 0.266
2.41TyrLeu: 2.41 ± 0.303
0.78TyrMet: 0.78 ± 0.144
1.524TyrAsn: 1.524 ± 0.23
1.347TyrPro: 1.347 ± 0.237
1.453TyrGln: 1.453 ± 0.216
1.914TyrArg: 1.914 ± 0.289
1.453TyrSer: 1.453 ± 0.209
1.701TyrThr: 1.701 ± 0.253
2.233TyrVal: 2.233 ± 0.327
0.567TyrTrp: 0.567 ± 0.142
0.992TyrTyr: 0.992 ± 0.187
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 124 proteins (28213 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski