Streptococcus satellite phage Javan243
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZGV2|A0A4D5ZGV2_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan243 OX=2558588 GN=JavanS243_0015 PE=4 SV=1
MM1 pKa = 7.09 EE2 pKa = 4.26 QATSRR7 pKa = 11.84 LNNVAVEE14 pKa = 4.05 AVEE17 pKa = 4.04 VLTAIMNDD25 pKa = 3.64 PTISPYY31 pKa = 10.94 ARR33 pKa = 11.84 QQSARR38 pKa = 11.84 TILEE42 pKa = 4.01 FAYY45 pKa = 9.67 KK46 pKa = 10.12 AYY48 pKa = 9.89 EE49 pKa = 3.87 NEE51 pKa = 4.25 AIIEE55 pKa = 4.08 KK56 pKa = 10.56 LEE58 pKa = 3.9 EE59 pKa = 4.52 LEE61 pKa = 4.34 TVISIDD67 pKa = 4.39 DD68 pKa = 3.56 KK69 pKa = 11.82 GII71 pKa = 3.54
Molecular weight: 7.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.48
IPC2_protein 4.406
IPC_protein 4.228
Toseland 4.088
ProMoST 4.317
Dawson 4.151
Bjellqvist 4.317
Wikipedia 3.999
Rodwell 4.062
Grimsley 3.999
Solomon 4.139
Lehninger 4.101
Nozaki 4.279
DTASelect 4.342
Thurlkill 4.088
EMBOSS 4.024
Sillero 4.329
Patrickios 3.808
IPC_peptide 4.151
IPC2_peptide 4.317
IPC2.peptide.svr19 4.264
Protein with the highest isoelectric point:
>tr|A0A4D5ZHS0|A0A4D5ZHS0_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan243 OX=2558588 GN=JavanS243_0011 PE=4 SV=1
MM1 pKa = 7.49 AVFFQKK7 pKa = 9.17 VHH9 pKa = 6.15 KK10 pKa = 10.1 NKK12 pKa = 10.21 AYY14 pKa = 9.9 KK15 pKa = 10.06 RR16 pKa = 11.84 LSQAFPRR23 pKa = 11.84 HH24 pKa = 5.45 SKK26 pKa = 10.68 EE27 pKa = 3.78 FLQMSWFSPLFYY39 pKa = 10.96
Molecular weight: 4.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.227
IPC2_protein 9.984
IPC_protein 10.189
Toseland 10.657
ProMoST 10.248
Dawson 10.774
Bjellqvist 10.379
Wikipedia 10.906
Rodwell 11.403
Grimsley 10.818
Solomon 10.804
Lehninger 10.789
Nozaki 10.613
DTASelect 10.379
Thurlkill 10.643
EMBOSS 11.038
Sillero 10.672
Patrickios 11.199
IPC_peptide 10.818
IPC2_peptide 8.96
IPC2.peptide.svr19 8.574
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17
0
17
2453
39
480
144.3
16.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.911 ± 0.515
0.448 ± 0.101
5.83 ± 0.694
8.113 ± 0.931
4.444 ± 0.438
4.28 ± 0.329
1.386 ± 0.3
7.379 ± 0.549
10.029 ± 0.662
9.499 ± 0.582
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.405 ± 0.285
5.177 ± 0.415
3.71 ± 0.636
3.995 ± 0.463
4.525 ± 0.329
5.137 ± 0.417
6.278 ± 0.394
5.585 ± 0.575
0.897 ± 0.194
4.974 ± 0.379
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here