Prevotella saccharolytica F0055
Average proteome isoelectric point is 7.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2553 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L1NMD2|L1NMD2_9BACT YWFCY domain-containing protein OS=Prevotella saccharolytica F0055 OX=1127699 GN=HMPREF9151_00011 PE=4 SV=1
MM1 pKa = 7.48 EE2 pKa = 5.94 AMDD5 pKa = 4.62 LNEE8 pKa = 3.34 ARR10 pKa = 11.84 IYY12 pKa = 10.4 VGTYY16 pKa = 9.78 AKK18 pKa = 10.5 YY19 pKa = 11.12 NNGTLQGEE27 pKa = 4.52 WVSLSDD33 pKa = 5.71 FYY35 pKa = 11.85 DD36 pKa = 3.75 LDD38 pKa = 4.13 GFLEE42 pKa = 4.62 YY43 pKa = 9.78 CTEE46 pKa = 3.53 IHH48 pKa = 6.69 EE49 pKa = 4.97 DD50 pKa = 3.77 EE51 pKa = 5.07 EE52 pKa = 4.35 EE53 pKa = 4.22 PEE55 pKa = 4.73 YY56 pKa = 10.66 IFQDD60 pKa = 3.41 WEE62 pKa = 4.4 NIPDD66 pKa = 3.9 SLIDD70 pKa = 3.73 EE71 pKa = 4.87 SNLEE75 pKa = 4.06 EE76 pKa = 4.52 NFFEE80 pKa = 6.16 LRR82 pKa = 11.84 DD83 pKa = 3.91 EE84 pKa = 4.33 LDD86 pKa = 3.48 KK87 pKa = 11.95 LNDD90 pKa = 3.69 TEE92 pKa = 6.42 KK93 pKa = 10.45 EE94 pKa = 4.29 AFWAWAEE101 pKa = 4.05 GNNIKK106 pKa = 9.04 LTQDD110 pKa = 2.98 AYY112 pKa = 11.25 RR113 pKa = 11.84 LVNSFQSAYY122 pKa = 9.12 IGSYY126 pKa = 9.92 ASKK129 pKa = 11.07 EE130 pKa = 3.99 EE131 pKa = 3.98 FAEE134 pKa = 4.0 EE135 pKa = 3.93 LVRR138 pKa = 11.84 MEE140 pKa = 4.91 NDD142 pKa = 3.02 LSDD145 pKa = 3.75 FALSYY150 pKa = 11.22 FDD152 pKa = 3.73 FSRR155 pKa = 11.84 YY156 pKa = 10.14 ADD158 pKa = 3.99 DD159 pKa = 6.6 LFDD162 pKa = 3.52 TDD164 pKa = 3.07 YY165 pKa = 10.26 WYY167 pKa = 11.07 KK168 pKa = 10.6 NGYY171 pKa = 7.26 VFRR174 pKa = 11.84 NEE176 pKa = 3.65
Molecular weight: 21.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.795
IPC_protein 3.77
Toseland 3.579
ProMoST 3.897
Dawson 3.745
Bjellqvist 3.923
Wikipedia 3.643
Rodwell 3.605
Grimsley 3.49
Solomon 3.732
Lehninger 3.694
Nozaki 3.859
DTASelect 4.024
Thurlkill 3.617
EMBOSS 3.656
Sillero 3.884
Patrickios 0.846
IPC_peptide 3.732
IPC2_peptide 3.872
IPC2.peptide.svr19 3.812
Protein with the highest isoelectric point:
>tr|L1NBU1|L1NBU1_9BACT OMP_b-brl_3 domain-containing protein OS=Prevotella saccharolytica F0055 OX=1127699 GN=HMPREF9151_01195 PE=4 SV=1
MM1 pKa = 7.56 LFALYY6 pKa = 10.3 VVLSLFHH13 pKa = 7.2 FFTFSPFHH21 pKa = 6.87 LFTFSPFHH29 pKa = 6.77 LFTFSPLFRR38 pKa = 11.84 GRR40 pKa = 11.84 LAGDD44 pKa = 3.14 VTINKK49 pKa = 8.16 CCKK52 pKa = 7.33 MNRR55 pKa = 11.84 RR56 pKa = 11.84 GVFSYY61 pKa = 10.82 RR62 pKa = 3.26
Molecular weight: 7.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 9.809
IPC_protein 10.657
Toseland 10.511
ProMoST 10.891
Dawson 10.672
Bjellqvist 10.438
Wikipedia 10.906
Rodwell 10.847
Grimsley 10.745
Solomon 10.789
Lehninger 10.745
Nozaki 10.555
DTASelect 10.423
Thurlkill 10.555
EMBOSS 10.921
Sillero 10.613
Patrickios 10.716
IPC_peptide 10.789
IPC2_peptide 9.78
IPC2.peptide.svr19 8.516
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2553
0
2553
834224
38
2515
326.8
36.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.29 ± 0.044
1.233 ± 0.018
5.443 ± 0.035
5.836 ± 0.052
4.647 ± 0.035
6.757 ± 0.049
2.032 ± 0.02
6.774 ± 0.042
6.542 ± 0.042
9.169 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.72 ± 0.023
5.222 ± 0.043
3.706 ± 0.025
3.697 ± 0.026
4.921 ± 0.036
5.96 ± 0.038
5.992 ± 0.036
6.414 ± 0.035
1.232 ± 0.022
4.414 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here