Alternanthera mosaic virus
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q52Z68|Q52Z68_9VIRU Triple gene block 2 protein OS=Alternanthera mosaic virus OX=85454 GN=TGB2 PE=4 SV=1
MM1 pKa = 7.91 PYY3 pKa = 10.13 LVEE6 pKa = 4.01 AAITVLACIGVLAALRR22 pKa = 11.84 PGSHH26 pKa = 7.51 PCTILLTGHH35 pKa = 6.52 SATISGDD42 pKa = 3.73 CGPVAPEE49 pKa = 4.31 TIRR52 pKa = 11.84 ALGDD56 pKa = 3.39 YY57 pKa = 8.97 LTGLRR62 pKa = 11.84 FF63 pKa = 3.39
Molecular weight: 6.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.531
IPC2_protein 5.753
IPC_protein 5.715
Toseland 6.033
ProMoST 6.033
Dawson 6.02
Bjellqvist 5.982
Wikipedia 6.046
Rodwell 6.02
Grimsley 6.109
Solomon 6.033
Lehninger 6.033
Nozaki 6.287
DTASelect 6.478
Thurlkill 6.504
EMBOSS 6.478
Sillero 6.402
Patrickios 0.782
IPC_peptide 6.046
IPC2_peptide 6.44
IPC2.peptide.svr19 6.347
Protein with the highest isoelectric point:
>tr|Q52Z69|Q52Z69_9VIRU Coat protein OS=Alternanthera mosaic virus OX=85454 GN=CP PE=3 SV=1
MM1 pKa = 7.51 SGLPHH6 pKa = 7.02 SLTPPADD13 pKa = 3.1 HH14 pKa = 6.84 SKK16 pKa = 10.42 PVLAAVVGVSLALVINSFLVYY37 pKa = 10.41 RR38 pKa = 11.84 LPSPGDD44 pKa = 3.84 NIHH47 pKa = 6.0 QLPFGGSYY55 pKa = 10.56 RR56 pKa = 11.84 DD57 pKa = 3.7 GTKK60 pKa = 10.27 SIHH63 pKa = 6.05 YY64 pKa = 8.29 NSPRR68 pKa = 11.84 AQSQISGASPFLIILILSALIYY90 pKa = 10.69 ALSCRR95 pKa = 11.84 GGHH98 pKa = 5.82 HH99 pKa = 6.78 RR100 pKa = 11.84 ARR102 pKa = 11.84 LHH104 pKa = 6.76 RR105 pKa = 11.84 CPCCSS110 pKa = 3.11
Molecular weight: 11.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.899
IPC2_protein 9.063
IPC_protein 9.326
Toseland 9.209
ProMoST 9.297
Dawson 9.604
Bjellqvist 9.619
Wikipedia 9.838
Rodwell 9.648
Grimsley 9.663
Solomon 9.75
Lehninger 9.706
Nozaki 9.692
DTASelect 9.487
Thurlkill 9.487
EMBOSS 9.706
Sillero 9.706
Patrickios 4.202
IPC_peptide 9.736
IPC2_peptide 9.136
IPC2.peptide.svr19 7.828
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2153
63
1541
430.6
48.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.128 ± 1.38
1.486 ± 0.476
4.18 ± 0.667
6.317 ± 1.385
4.691 ± 0.555
5.527 ± 0.71
3.53 ± 0.779
5.388 ± 0.443
6.038 ± 1.72
9.94 ± 0.859
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.719 ± 0.363
3.809 ± 0.887
7.06 ± 0.734
4.459 ± 0.617
4.32 ± 0.654
7.71 ± 0.943
6.131 ± 0.866
5.156 ± 0.593
1.115 ± 0.341
3.298 ± 0.577
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here