Thermofilum uzonense

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Thermoproteales; Thermofilaceae; Thermofilum

Average proteome isoelectric point is 7.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1454 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F7CLF5|A0A0F7CLF5_9CREN Sugar transporter OS=Thermofilum uzonense OX=1550241 GN=MA03_02565 PE=3 SV=1
MM1 pKa = 7.68RR2 pKa = 11.84GKK4 pKa = 10.52SRR6 pKa = 11.84DD7 pKa = 3.66CSSLCEE13 pKa = 4.08EE14 pKa = 4.93LVEE17 pKa = 4.17EE18 pKa = 4.78CQRR21 pKa = 11.84KK22 pKa = 8.91CDD24 pKa = 3.96KK25 pKa = 10.65EE26 pKa = 4.29VSEE29 pKa = 5.12CMEE32 pKa = 4.56DD33 pKa = 3.44CAGLEE38 pKa = 4.5DD39 pKa = 4.64EE40 pKa = 5.16SCLDD44 pKa = 3.51VCEE47 pKa = 4.37EE48 pKa = 4.0TCEE51 pKa = 4.06RR52 pKa = 11.84MSKK55 pKa = 10.71ACIDD59 pKa = 3.43FCKK62 pKa = 10.57NVEE65 pKa = 4.25NKK67 pKa = 10.1CQEE70 pKa = 3.81LCEE73 pKa = 4.23EE74 pKa = 4.38VEE76 pKa = 4.36EE77 pKa = 4.4

Molecular weight: 8.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F7FJ69|A0A0F7FJ69_9CREN DNA repair and recombination protein RadA OS=Thermofilum uzonense OX=1550241 GN=radA PE=3 SV=1
MM1 pKa = 6.7PTKK4 pKa = 10.61VRR6 pKa = 11.84IRR8 pKa = 11.84LSSTSVEE15 pKa = 4.42DD16 pKa = 4.04LNAVCKK22 pKa = 10.64DD23 pKa = 2.79IVEE26 pKa = 4.17IAKK29 pKa = 9.04RR30 pKa = 11.84TGVKK34 pKa = 9.58IRR36 pKa = 11.84GPIPLPVQVARR47 pKa = 11.84VVTRR51 pKa = 11.84RR52 pKa = 11.84APSGQGYY59 pKa = 6.96EE60 pKa = 4.02TFDD63 pKa = 3.4RR64 pKa = 11.84FEE66 pKa = 4.11LRR68 pKa = 11.84IHH70 pKa = 6.5KK71 pKa = 10.25RR72 pKa = 11.84LIDD75 pKa = 3.47MDD77 pKa = 4.04ADD79 pKa = 3.54EE80 pKa = 4.67RR81 pKa = 11.84ATRR84 pKa = 11.84LLLRR88 pKa = 11.84TRR90 pKa = 11.84VPPTVRR96 pKa = 11.84VEE98 pKa = 4.03IEE100 pKa = 4.22VVV102 pKa = 3.04

Molecular weight: 11.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1454

0

1454

421868

52

1723

290.1

32.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.623 ± 0.066

0.654 ± 0.021

4.289 ± 0.042

7.328 ± 0.078

3.967 ± 0.039

7.219 ± 0.054

1.573 ± 0.025

6.995 ± 0.05

5.825 ± 0.061

11.608 ± 0.087

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.034 ± 0.027

2.961 ± 0.037

4.687 ± 0.041

2.256 ± 0.033

6.204 ± 0.065

6.574 ± 0.048

4.777 ± 0.057

8.403 ± 0.053

1.215 ± 0.025

3.809 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski