Amino acid dipepetide frequency for Thermofilum uzonense

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.995AlaAla: 5.995 ± 0.149
0.51AlaCys: 0.51 ± 0.034
2.724AlaAsp: 2.724 ± 0.079
4.838AlaGlu: 4.838 ± 0.112
3.285AlaPhe: 3.285 ± 0.085
5.45AlaGly: 5.45 ± 0.139
1.244AlaHis: 1.244 ± 0.051
4.847AlaIle: 4.847 ± 0.118
3.831AlaLys: 3.831 ± 0.104
10.07AlaLeu: 10.07 ± 0.168
1.816AlaMet: 1.816 ± 0.065
1.61AlaAsn: 1.61 ± 0.067
2.757AlaPro: 2.757 ± 0.087
1.759AlaGln: 1.759 ± 0.068
5.461AlaArg: 5.461 ± 0.142
5.703AlaSer: 5.703 ± 0.137
3.655AlaThr: 3.655 ± 0.112
6.649AlaVal: 6.649 ± 0.147
1.015AlaTrp: 1.015 ± 0.047
3.018AlaTyr: 3.018 ± 0.082
0.0AlaXaa: 0.0 ± 0.0
Cys
0.339CysAla: 0.339 ± 0.028
0.066CysCys: 0.066 ± 0.013
0.261CysAsp: 0.261 ± 0.023
0.351CysGlu: 0.351 ± 0.034
0.211CysPhe: 0.211 ± 0.021
0.825CysGly: 0.825 ± 0.044
0.128CysHis: 0.128 ± 0.019
0.417CysIle: 0.417 ± 0.034
0.329CysLys: 0.329 ± 0.029
0.569CysLeu: 0.569 ± 0.033
0.09CysMet: 0.09 ± 0.014
0.183CysAsn: 0.183 ± 0.019
0.692CysPro: 0.692 ± 0.048
0.145CysGln: 0.145 ± 0.02
0.429CysArg: 0.429 ± 0.035
0.469CysSer: 0.469 ± 0.034
0.301CysThr: 0.301 ± 0.027
0.412CysVal: 0.412 ± 0.034
0.078CysTrp: 0.078 ± 0.013
0.239CysTyr: 0.239 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
3.231AspAla: 3.231 ± 0.08
0.277AspCys: 0.277 ± 0.023
1.633AspAsp: 1.633 ± 0.069
3.482AspGlu: 3.482 ± 0.1
1.951AspPhe: 1.951 ± 0.068
2.702AspGly: 2.702 ± 0.07
0.666AspHis: 0.666 ± 0.04
3.447AspIle: 3.447 ± 0.101
2.588AspLys: 2.588 ± 0.071
4.729AspLeu: 4.729 ± 0.102
0.792AspMet: 0.792 ± 0.043
1.026AspAsn: 1.026 ± 0.044
2.837AspPro: 2.837 ± 0.09
0.799AspGln: 0.799 ± 0.047
1.996AspArg: 1.996 ± 0.079
2.245AspSer: 2.245 ± 0.072
1.979AspThr: 1.979 ± 0.071
3.8AspVal: 3.8 ± 0.108
0.702AspTrp: 0.702 ± 0.041
2.005AspTyr: 2.005 ± 0.069
0.0AspXaa: 0.0 ± 0.0
Glu
6.429GluAla: 6.429 ± 0.118
0.384GluCys: 0.384 ± 0.031
3.338GluAsp: 3.338 ± 0.097
7.076GluGlu: 7.076 ± 0.176
2.51GluPhe: 2.51 ± 0.081
4.596GluGly: 4.596 ± 0.098
1.114GluHis: 1.114 ± 0.057
5.277GluIle: 5.277 ± 0.127
6.258GluLys: 6.258 ± 0.16
7.694GluLeu: 7.694 ± 0.165
1.451GluMet: 1.451 ± 0.06
2.534GluAsn: 2.534 ± 0.08
2.638GluPro: 2.638 ± 0.097
1.425GluGln: 1.425 ± 0.065
4.319GluArg: 4.319 ± 0.114
3.748GluSer: 3.748 ± 0.1
2.937GluThr: 2.937 ± 0.088
6.142GluVal: 6.142 ± 0.141
0.853GluTrp: 0.853 ± 0.044
2.56GluTyr: 2.56 ± 0.083
0.0GluXaa: 0.0 ± 0.0
Phe
2.724PheAla: 2.724 ± 0.09
0.244PheCys: 0.244 ± 0.024
1.747PheAsp: 1.747 ± 0.069
2.61PheGlu: 2.61 ± 0.075
1.818PhePhe: 1.818 ± 0.074
2.373PheGly: 2.373 ± 0.091
0.607PheHis: 0.607 ± 0.042
2.826PheIle: 2.826 ± 0.102
2.323PheLys: 2.323 ± 0.07
4.966PheLeu: 4.966 ± 0.119
0.877PheMet: 0.877 ± 0.051
1.448PheAsn: 1.448 ± 0.061
1.662PhePro: 1.662 ± 0.067
1.038PheGln: 1.038 ± 0.049
2.081PheArg: 2.081 ± 0.076
3.034PheSer: 3.034 ± 0.082
2.432PheThr: 2.432 ± 0.079
2.636PheVal: 2.636 ± 0.08
0.462PheTrp: 0.462 ± 0.035
1.766PheTyr: 1.766 ± 0.073
0.0PheXaa: 0.0 ± 0.0
Gly
4.966GlyAla: 4.966 ± 0.137
0.521GlyCys: 0.521 ± 0.04
3.245GlyAsp: 3.245 ± 0.09
5.149GlyGlu: 5.149 ± 0.116
3.601GlyPhe: 3.601 ± 0.094
5.31GlyGly: 5.31 ± 0.144
1.086GlyHis: 1.086 ± 0.048
4.997GlyIle: 4.997 ± 0.119
4.629GlyLys: 4.629 ± 0.13
8.313GlyLeu: 8.313 ± 0.144
1.529GlyMet: 1.529 ± 0.058
1.96GlyAsn: 1.96 ± 0.077
2.261GlyPro: 2.261 ± 0.073
1.403GlyGln: 1.403 ± 0.06
4.428GlyArg: 4.428 ± 0.109
4.577GlySer: 4.577 ± 0.098
3.103GlyThr: 3.103 ± 0.1
6.896GlyVal: 6.896 ± 0.137
1.071GlyTrp: 1.071 ± 0.058
3.143GlyTyr: 3.143 ± 0.077
0.0GlyXaa: 0.0 ± 0.0
His
1.325HisAla: 1.325 ± 0.061
0.152HisCys: 0.152 ± 0.022
0.699HisAsp: 0.699 ± 0.047
1.095HisGlu: 1.095 ± 0.058
0.569HisPhe: 0.569 ± 0.038
1.285HisGly: 1.285 ± 0.054
0.303HisHis: 0.303 ± 0.031
1.012HisIle: 1.012 ± 0.048
0.683HisLys: 0.683 ± 0.035
1.704HisLeu: 1.704 ± 0.064
0.303HisMet: 0.303 ± 0.026
0.427HisAsn: 0.427 ± 0.031
1.052HisPro: 1.052 ± 0.059
0.32HisGln: 0.32 ± 0.027
0.929HisArg: 0.929 ± 0.042
0.884HisSer: 0.884 ± 0.04
0.706HisThr: 0.706 ± 0.044
1.5HisVal: 1.5 ± 0.06
0.199HisTrp: 0.199 ± 0.02
0.583HisTyr: 0.583 ± 0.035
0.0HisXaa: 0.0 ± 0.0
Ile
6.021IleAla: 6.021 ± 0.111
0.36IleCys: 0.36 ± 0.029
3.541IleAsp: 3.541 ± 0.086
5.028IleGlu: 5.028 ± 0.114
2.648IlePhe: 2.648 ± 0.077
4.755IleGly: 4.755 ± 0.101
1.074IleHis: 1.074 ± 0.048
5.265IleIle: 5.265 ± 0.111
3.485IleLys: 3.485 ± 0.082
8.012IleLeu: 8.012 ± 0.148
1.292IleMet: 1.292 ± 0.059
1.975IleAsn: 1.975 ± 0.071
3.681IlePro: 3.681 ± 0.086
1.588IleGln: 1.588 ± 0.063
3.84IleArg: 3.84 ± 0.111
4.748IleSer: 4.748 ± 0.114
3.435IleThr: 3.435 ± 0.094
5.988IleVal: 5.988 ± 0.113
0.628IleTrp: 0.628 ± 0.042
2.591IleTyr: 2.591 ± 0.081
0.0IleXaa: 0.0 ± 0.0
Lys
4.847LysAla: 4.847 ± 0.111
0.484LysCys: 0.484 ± 0.038
2.311LysAsp: 2.311 ± 0.073
4.158LysGlu: 4.158 ± 0.137
1.548LysPhe: 1.548 ± 0.067
4.501LysGly: 4.501 ± 0.1
0.943LysHis: 0.943 ± 0.047
4.674LysIle: 4.674 ± 0.123
3.589LysLys: 3.589 ± 0.122
6.369LysLeu: 6.369 ± 0.131
1.135LysMet: 1.135 ± 0.051
1.946LysAsn: 1.946 ± 0.08
2.93LysPro: 2.93 ± 0.077
1.24LysGln: 1.24 ± 0.055
3.634LysArg: 3.634 ± 0.103
3.172LysSer: 3.172 ± 0.097
3.183LysThr: 3.183 ± 0.099
4.919LysVal: 4.919 ± 0.12
0.562LysTrp: 0.562 ± 0.04
2.1LysTyr: 2.1 ± 0.066
0.0LysXaa: 0.0 ± 0.0
Leu
10.081LeuAla: 10.081 ± 0.198
0.612LeuCys: 0.612 ± 0.041
5.092LeuAsp: 5.092 ± 0.107
9.041LeuGlu: 9.041 ± 0.182
3.928LeuPhe: 3.928 ± 0.111
8.415LeuGly: 8.415 ± 0.143
1.726LeuHis: 1.726 ± 0.073
7.147LeuIle: 7.147 ± 0.138
7.163LeuLys: 7.163 ± 0.145
13.052LeuLeu: 13.052 ± 0.249
2.157LeuMet: 2.157 ± 0.079
3.733LeuAsn: 3.733 ± 0.101
5.011LeuPro: 5.011 ± 0.106
2.833LeuGln: 2.833 ± 0.1
7.607LeuArg: 7.607 ± 0.137
8.711LeuSer: 8.711 ± 0.166
5.544LeuThr: 5.544 ± 0.13
8.913LeuVal: 8.913 ± 0.143
1.152LeuTrp: 1.152 ± 0.048
4.16LeuTyr: 4.16 ± 0.113
0.0LeuXaa: 0.0 ± 0.0
Met
1.586MetAla: 1.586 ± 0.064
0.121MetCys: 0.121 ± 0.019
0.922MetAsp: 0.922 ± 0.041
1.157MetGlu: 1.157 ± 0.047
0.732MetPhe: 0.732 ± 0.043
1.702MetGly: 1.702 ± 0.07
0.356MetHis: 0.356 ± 0.032
1.375MetIle: 1.375 ± 0.058
1.178MetLys: 1.178 ± 0.048
2.453MetLeu: 2.453 ± 0.089
0.391MetMet: 0.391 ± 0.035
0.638MetAsn: 0.638 ± 0.037
0.977MetPro: 0.977 ± 0.046
0.479MetGln: 0.479 ± 0.035
1.434MetArg: 1.434 ± 0.062
1.591MetSer: 1.591 ± 0.058
0.955MetThr: 0.955 ± 0.056
1.429MetVal: 1.429 ± 0.053
0.225MetTrp: 0.225 ± 0.026
0.635MetTyr: 0.635 ± 0.038
0.0MetXaa: 0.0 ± 0.0
Asn
2.171AsnAla: 2.171 ± 0.076
0.254AsnCys: 0.254 ± 0.03
1.055AsnAsp: 1.055 ± 0.048
1.638AsnGlu: 1.638 ± 0.069
1.09AsnPhe: 1.09 ± 0.052
2.065AsnGly: 2.065 ± 0.087
0.405AsnHis: 0.405 ± 0.031
2.712AsnIle: 2.712 ± 0.082
1.429AsnLys: 1.429 ± 0.055
3.439AsnLeu: 3.439 ± 0.09
0.659AsnMet: 0.659 ± 0.039
0.972AsnAsn: 0.972 ± 0.051
2.176AsnPro: 2.176 ± 0.081
0.569AsnGln: 0.569 ± 0.041
1.361AsnArg: 1.361 ± 0.057
1.688AsnSer: 1.688 ± 0.069
1.645AsnThr: 1.645 ± 0.058
2.714AsnVal: 2.714 ± 0.083
0.408AsnTrp: 0.408 ± 0.027
1.162AsnTyr: 1.162 ± 0.058
0.0AsnXaa: 0.0 ± 0.0
Pro
2.762ProAla: 2.762 ± 0.091
0.308ProCys: 0.308 ± 0.027
2.25ProAsp: 2.25 ± 0.073
3.724ProGlu: 3.724 ± 0.106
1.88ProPhe: 1.88 ± 0.066
3.778ProGly: 3.778 ± 0.093
0.917ProHis: 0.917 ± 0.045
2.664ProIle: 2.664 ± 0.072
2.015ProLys: 2.015 ± 0.085
5.362ProLeu: 5.362 ± 0.128
0.787ProMet: 0.787 ± 0.044
1.249ProAsn: 1.249 ± 0.063
2.873ProPro: 2.873 ± 0.101
1.306ProGln: 1.306 ± 0.069
2.923ProArg: 2.923 ± 0.087
3.442ProSer: 3.442 ± 0.1
2.084ProThr: 2.084 ± 0.072
4.058ProVal: 4.058 ± 0.092
0.661ProTrp: 0.661 ± 0.043
1.823ProTyr: 1.823 ± 0.068
0.0ProXaa: 0.0 ± 0.0
Gln
2.039GlnAla: 2.039 ± 0.069
0.168GlnCys: 0.168 ± 0.019
0.91GlnAsp: 0.91 ± 0.049
1.685GlnGlu: 1.685 ± 0.069
0.612GlnPhe: 0.612 ± 0.044
1.707GlnGly: 1.707 ± 0.062
0.32GlnHis: 0.32 ± 0.028
1.294GlnIle: 1.294 ± 0.059
1.185GlnLys: 1.185 ± 0.061
2.707GlnLeu: 2.707 ± 0.084
0.514GlnMet: 0.514 ± 0.038
0.702GlnAsn: 0.702 ± 0.046
1.152GlnPro: 1.152 ± 0.062
0.728GlnGln: 0.728 ± 0.055
1.643GlnArg: 1.643 ± 0.072
1.23GlnSer: 1.23 ± 0.05
1.01GlnThr: 1.01 ± 0.054
2.031GlnVal: 2.031 ± 0.067
0.261GlnTrp: 0.261 ± 0.031
0.666GlnTyr: 0.666 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
4.264ArgAla: 4.264 ± 0.121
0.422ArgCys: 0.422 ± 0.035
2.771ArgAsp: 2.771 ± 0.09
5.969ArgGlu: 5.969 ± 0.146
2.271ArgPhe: 2.271 ± 0.075
4.599ArgGly: 4.599 ± 0.11
0.977ArgHis: 0.977 ± 0.052
4.786ArgIle: 4.786 ± 0.115
3.774ArgLys: 3.774 ± 0.114
6.988ArgLeu: 6.988 ± 0.153
1.233ArgMet: 1.233 ± 0.055
1.835ArgAsn: 1.835 ± 0.062
2.37ArgPro: 2.37 ± 0.078
1.24ArgGln: 1.24 ± 0.056
4.388ArgArg: 4.388 ± 0.123
3.119ArgSer: 3.119 ± 0.093
2.145ArgThr: 2.145 ± 0.064
6.137ArgVal: 6.137 ± 0.121
0.704ArgTrp: 0.704 ± 0.043
2.05ArgTyr: 2.05 ± 0.072
0.0ArgXaa: 0.0 ± 0.0
Ser
3.731SerAla: 3.731 ± 0.112
0.439SerCys: 0.439 ± 0.036
2.487SerAsp: 2.487 ± 0.089
4.267SerGlu: 4.267 ± 0.108
3.07SerPhe: 3.07 ± 0.081
5.089SerGly: 5.089 ± 0.113
0.969SerHis: 0.969 ± 0.049
4.964SerIle: 4.964 ± 0.114
3.686SerLys: 3.686 ± 0.098
8.548SerLeu: 8.548 ± 0.202
1.519SerMet: 1.519 ± 0.055
1.726SerAsn: 1.726 ± 0.061
3.055SerPro: 3.055 ± 0.081
1.745SerGln: 1.745 ± 0.061
4.487SerArg: 4.487 ± 0.113
4.793SerSer: 4.793 ± 0.127
3.373SerThr: 3.373 ± 0.106
4.499SerVal: 4.499 ± 0.115
0.832SerTrp: 0.832 ± 0.041
2.463SerTyr: 2.463 ± 0.081
0.0SerXaa: 0.0 ± 0.0
Thr
3.532ThrAla: 3.532 ± 0.095
0.337ThrCys: 0.337 ± 0.029
1.6ThrAsp: 1.6 ± 0.056
2.186ThrGlu: 2.186 ± 0.076
1.939ThrPhe: 1.939 ± 0.068
4.478ThrGly: 4.478 ± 0.114
0.901ThrHis: 0.901 ± 0.039
3.335ThrIle: 3.335 ± 0.097
1.797ThrLys: 1.797 ± 0.054
6.405ThrLeu: 6.405 ± 0.123
0.894ThrMet: 0.894 ± 0.046
1.14ThrAsn: 1.14 ± 0.053
3.048ThrPro: 3.048 ± 0.106
1.093ThrGln: 1.093 ± 0.052
3.098ThrArg: 3.098 ± 0.086
3.176ThrSer: 3.176 ± 0.094
2.615ThrThr: 2.615 ± 0.107
3.864ThrVal: 3.864 ± 0.108
0.6ThrTrp: 0.6 ± 0.041
1.73ThrTyr: 1.73 ± 0.08
0.0ThrXaa: 0.0 ± 0.0
Val
6.585ValAla: 6.585 ± 0.142
0.486ValCys: 0.486 ± 0.038
4.096ValAsp: 4.096 ± 0.099
6.68ValGlu: 6.68 ± 0.137
3.956ValPhe: 3.956 ± 0.112
4.805ValGly: 4.805 ± 0.122
1.207ValHis: 1.207 ± 0.057
5.554ValIle: 5.554 ± 0.111
5.755ValLys: 5.755 ± 0.123
9.055ValLeu: 9.055 ± 0.141
1.733ValMet: 1.733 ± 0.068
2.759ValAsn: 2.759 ± 0.083
3.345ValPro: 3.345 ± 0.101
1.802ValGln: 1.802 ± 0.063
4.641ValArg: 4.641 ± 0.107
5.845ValSer: 5.845 ± 0.13
3.985ValThr: 3.985 ± 0.119
7.291ValVal: 7.291 ± 0.149
0.943ValTrp: 0.943 ± 0.055
3.503ValTyr: 3.503 ± 0.088
0.0ValXaa: 0.0 ± 0.0
Trp
0.756TrpAla: 0.756 ± 0.039
0.088TrpCys: 0.088 ± 0.014
0.557TrpAsp: 0.557 ± 0.041
0.834TrpGlu: 0.834 ± 0.051
0.505TrpPhe: 0.505 ± 0.034
0.941TrpGly: 0.941 ± 0.048
0.235TrpHis: 0.235 ± 0.023
0.891TrpIle: 0.891 ± 0.056
0.529TrpLys: 0.529 ± 0.038
1.413TrpLeu: 1.413 ± 0.06
0.277TrpMet: 0.277 ± 0.027
0.434TrpAsn: 0.434 ± 0.032
0.462TrpPro: 0.462 ± 0.035
0.282TrpGln: 0.282 ± 0.029
1.034TrpArg: 1.034 ± 0.044
0.86TrpSer: 0.86 ± 0.044
0.519TrpThr: 0.519 ± 0.031
0.908TrpVal: 0.908 ± 0.052
0.213TrpTrp: 0.213 ± 0.026
0.41TrpTyr: 0.41 ± 0.032
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.852TyrAla: 2.852 ± 0.083
0.303TyrCys: 0.303 ± 0.027
1.65TyrAsp: 1.65 ± 0.07
2.316TyrGlu: 2.316 ± 0.071
1.55TyrPhe: 1.55 ± 0.067
2.814TyrGly: 2.814 ± 0.077
0.555TyrHis: 0.555 ± 0.04
2.47TyrIle: 2.47 ± 0.072
2.02TyrLys: 2.02 ± 0.077
4.229TyrLeu: 4.229 ± 0.113
0.884TyrMet: 0.884 ± 0.051
1.316TyrAsn: 1.316 ± 0.056
1.884TyrPro: 1.884 ± 0.07
0.773TyrGln: 0.773 ± 0.04
2.309TyrArg: 2.309 ± 0.078
2.705TyrSer: 2.705 ± 0.083
2.159TyrThr: 2.159 ± 0.075
3.243TyrVal: 3.243 ± 0.087
0.578TyrTrp: 0.578 ± 0.038
1.482TyrTyr: 1.482 ± 0.061
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1454 proteins (421868 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski