Corynebacterium frankenforstense DSM 45800
Average proteome isoelectric point is 5.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1801 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L7CS68|A0A1L7CS68_9CORY PspC domain-containing protein OS=Corynebacterium frankenforstense DSM 45800 OX=1437875 GN=CFRA_04690 PE=4 SV=1
MM1 pKa = 7.76 SDD3 pKa = 3.57 DD4 pKa = 4.37 LKK6 pKa = 11.46 SIGLDD11 pKa = 3.22 FDD13 pKa = 3.77 RR14 pKa = 11.84 WQDD17 pKa = 3.24 AVEE20 pKa = 4.03 AAIGTDD26 pKa = 3.01 RR27 pKa = 11.84 LAVTGAVRR35 pKa = 11.84 GGQLIQYY42 pKa = 8.71 SDD44 pKa = 3.61 PSGAQINILAVEE56 pKa = 5.27 PYY58 pKa = 9.01 STWAGFDD65 pKa = 3.5 AQSQTTGTLDD75 pKa = 3.24 MVNDD79 pKa = 3.5 VLALVDD85 pKa = 3.64 VTDD88 pKa = 4.06 PAGQEE93 pKa = 4.06 LGTITANLSQGPLFYY108 pKa = 10.63 EE109 pKa = 4.37 VGPQPEE115 pKa = 4.26 QPLGITALALDD126 pKa = 4.01 AKK128 pKa = 10.24 VYY130 pKa = 10.23 PSDD133 pKa = 3.72 VEE135 pKa = 4.21 YY136 pKa = 10.93 EE137 pKa = 4.09 KK138 pKa = 10.92 ATGEE142 pKa = 4.31 VFGVVYY148 pKa = 10.77 SEE150 pKa = 4.34 GAQLVASGSGAAVPDD165 pKa = 4.12 AGAQFSARR173 pKa = 11.84 VITAEE178 pKa = 4.33 RR179 pKa = 11.84 RR180 pKa = 11.84 TSDD183 pKa = 3.04 LTGQAFIHH191 pKa = 5.99 ATVEE195 pKa = 4.44 GPFPFDD201 pKa = 2.91 VCLPDD206 pKa = 4.71 AADD209 pKa = 3.67 VPQPGAVIAGTALMAASVLVTGGGCGGSGGCGCGAGGCGCGGHH252 pKa = 6.68
Molecular weight: 25.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.821
IPC_protein 3.808
Toseland 3.592
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.745
Rodwell 3.643
Grimsley 3.503
Solomon 3.795
Lehninger 3.757
Nozaki 3.923
DTASelect 4.164
Thurlkill 3.656
EMBOSS 3.757
Sillero 3.935
Patrickios 0.769
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.822
Protein with the highest isoelectric point:
>tr|A0A1L7CV99|A0A1L7CV99_9CORY Antibiotic biosynthesis monooxygenase OS=Corynebacterium frankenforstense DSM 45800 OX=1437875 GN=CFRA_11350 PE=4 SV=1
MM1 pKa = 7.53 SKK3 pKa = 10.53 GKK5 pKa = 8.66 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 AKK17 pKa = 9.81 KK18 pKa = 9.26 HH19 pKa = 4.29 GFRR22 pKa = 11.84 TRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVAARR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 9.72 GRR42 pKa = 11.84 AKK44 pKa = 9.63 LTAA47 pKa = 4.21
Molecular weight: 5.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.33
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.568
Rodwell 12.749
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.486
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.236
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1801
0
1801
600005
40
3139
333.2
35.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.231 ± 0.105
0.716 ± 0.016
6.571 ± 0.05
6.739 ± 0.058
3.041 ± 0.035
9.324 ± 0.053
2.168 ± 0.029
3.946 ± 0.046
2.504 ± 0.054
9.485 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.014 ± 0.024
2.248 ± 0.028
5.212 ± 0.044
2.576 ± 0.029
7.209 ± 0.061
4.883 ± 0.031
5.895 ± 0.037
8.862 ± 0.056
1.335 ± 0.023
2.041 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here