Gordonia phage GTE6
Average proteome isoelectric point is 5.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K0MWT5|A0A0K0MWT5_9CAUD Uncharacterized protein OS=Gordonia phage GTE6 OX=1647474 GN=GTE6_65 PE=4 SV=1
MM1 pKa = 7.61 TDD3 pKa = 3.58 LMTTIYY9 pKa = 9.84 QHH11 pKa = 7.53 PDD13 pKa = 2.78 TGHH16 pKa = 7.08 LATYY20 pKa = 9.84 SPRR23 pKa = 11.84 AGNDD27 pKa = 3.37 DD28 pKa = 3.92 PNPWVEE34 pKa = 4.13 YY35 pKa = 10.03 ACTPFAFSAVGRR47 pKa = 11.84 HH48 pKa = 5.9 PEE50 pKa = 3.95 LPAGYY55 pKa = 9.34 LALLLVPPPAVGTILDD71 pKa = 4.09 GLIDD75 pKa = 4.45 AAPADD80 pKa = 3.95 AAVLAGTEE88 pKa = 4.16 PPRR91 pKa = 11.84 TIPDD95 pKa = 3.7 GPAMFVVEE103 pKa = 4.61 CLCTDD108 pKa = 3.79 AEE110 pKa = 4.6 SIRR113 pKa = 11.84 CRR115 pKa = 11.84 AHH117 pKa = 6.94 GPSPDD122 pKa = 3.6 DD123 pKa = 3.51 WADD126 pKa = 3.45 QVDD129 pKa = 4.2 DD130 pKa = 3.52 QRR132 pKa = 11.84 KK133 pKa = 8.57 RR134 pKa = 11.84 ADD136 pKa = 3.19 AAEE139 pKa = 4.51 ADD141 pKa = 4.17 LAAVDD146 pKa = 3.57 QALAEE151 pKa = 4.21 LRR153 pKa = 11.84 DD154 pKa = 3.83 QISRR158 pKa = 11.84 QQ159 pKa = 3.39
Molecular weight: 16.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.153
IPC2_protein 4.139
IPC_protein 4.126
Toseland 3.91
ProMoST 4.291
Dawson 4.126
Bjellqvist 4.279
Wikipedia 4.075
Rodwell 3.948
Grimsley 3.821
Solomon 4.113
Lehninger 4.075
Nozaki 4.24
DTASelect 4.507
Thurlkill 3.961
EMBOSS 4.075
Sillero 4.253
Patrickios 3.312
IPC_peptide 4.113
IPC2_peptide 4.228
IPC2.peptide.svr19 4.169
Protein with the highest isoelectric point:
>tr|A0A0K0MWW5|A0A0K0MWW5_9CAUD Uncharacterized protein OS=Gordonia phage GTE6 OX=1647474 GN=GTE6_32 PE=4 SV=1
MM1 pKa = 7.25 TVRR4 pKa = 11.84 INSGNIARR12 pKa = 11.84 DD13 pKa = 3.3 KK14 pKa = 11.41 ARR16 pKa = 11.84 IGQQWGHH23 pKa = 6.76 RR24 pKa = 11.84 IGGRR28 pKa = 11.84 VQNAARR34 pKa = 11.84 KK35 pKa = 6.94 RR36 pKa = 11.84 TPVDD40 pKa = 2.92 TGALRR45 pKa = 11.84 TSIEE49 pKa = 4.01 YY50 pKa = 9.47 TVDD53 pKa = 3.03 VRR55 pKa = 11.84 PDD57 pKa = 3.42 STHH60 pKa = 4.29 VTIGSPLEE68 pKa = 3.76 YY69 pKa = 10.24 ARR71 pKa = 11.84 YY72 pKa = 7.96 IHH74 pKa = 7.24 EE75 pKa = 4.4 GTGIYY80 pKa = 9.73 GPKK83 pKa = 8.6 GTPIVPVTRR92 pKa = 11.84 QVLKK96 pKa = 10.41 FQVKK100 pKa = 9.52 GSTGKK105 pKa = 10.24 RR106 pKa = 11.84 RR107 pKa = 11.84 GKK109 pKa = 9.38 DD110 pKa = 3.36 AKK112 pKa = 9.1 WVYY115 pKa = 10.08 AKK117 pKa = 10.12 SVKK120 pKa = 9.65 GVKK123 pKa = 9.62 PNPFLVDD130 pKa = 3.34 ALVDD134 pKa = 3.47 VMGQVNRR141 pKa = 11.84 LRR143 pKa = 4.49
Molecular weight: 15.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.309
IPC2_protein 9.984
IPC_protein 10.891
Toseland 11.038
ProMoST 11.082
Dawson 11.096
Bjellqvist 10.847
Wikipedia 11.345
Rodwell 11.316
Grimsley 11.14
Solomon 11.286
Lehninger 11.242
Nozaki 11.008
DTASelect 10.847
Thurlkill 11.023
EMBOSS 11.447
Sillero 11.038
Patrickios 11.038
IPC_peptide 11.301
IPC2_peptide 9.765
IPC2.peptide.svr19 8.5
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
86
0
86
18289
48
1706
212.7
22.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.696 ± 0.428
0.826 ± 0.129
7.529 ± 0.472
5.615 ± 0.401
2.504 ± 0.205
8.825 ± 0.441
1.941 ± 0.199
4.434 ± 0.195
2.849 ± 0.211
7.742 ± 0.254
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.947 ± 0.103
2.717 ± 0.172
5.911 ± 0.242
3.767 ± 0.155
6.889 ± 0.491
4.937 ± 0.251
7.283 ± 0.298
7.234 ± 0.224
2.105 ± 0.14
2.247 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here