Amino acid dipepetide frequency for Gordonia phage GTE6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.084AlaAla: 19.084 ± 1.557
0.656AlaCys: 0.656 ± 0.18
9.897AlaAsp: 9.897 ± 0.895
9.077AlaGlu: 9.077 ± 1.043
2.843AlaPhe: 2.843 ± 0.571
12.686AlaGly: 12.686 ± 1.062
2.57AlaHis: 2.57 ± 0.412
6.179AlaIle: 6.179 ± 0.856
4.538AlaLys: 4.538 ± 0.617
8.968AlaLeu: 8.968 ± 0.681
2.078AlaMet: 2.078 ± 0.316
3.117AlaAsn: 3.117 ± 0.38
5.413AlaPro: 5.413 ± 0.529
4.812AlaGln: 4.812 ± 0.594
7.327AlaArg: 7.327 ± 0.82
6.343AlaSer: 6.343 ± 0.53
7.874AlaThr: 7.874 ± 0.617
8.585AlaVal: 8.585 ± 1.226
2.515AlaTrp: 2.515 ± 0.329
2.406AlaTyr: 2.406 ± 0.483
0.0AlaXaa: 0.0 ± 0.0
Cys
0.93CysAla: 0.93 ± 0.263
0.055CysCys: 0.055 ± 0.055
0.547CysAsp: 0.547 ± 0.162
0.547CysGlu: 0.547 ± 0.158
0.273CysPhe: 0.273 ± 0.124
0.82CysGly: 0.82 ± 0.267
0.164CysHis: 0.164 ± 0.094
0.055CysIle: 0.055 ± 0.051
0.273CysLys: 0.273 ± 0.126
0.601CysLeu: 0.601 ± 0.162
0.055CysMet: 0.055 ± 0.051
0.273CysAsn: 0.273 ± 0.158
0.82CysPro: 0.82 ± 0.246
0.164CysGln: 0.164 ± 0.097
0.82CysArg: 0.82 ± 0.245
0.437CysSer: 0.437 ± 0.17
0.711CysThr: 0.711 ± 0.236
0.328CysVal: 0.328 ± 0.132
0.164CysTrp: 0.164 ± 0.085
0.219CysTyr: 0.219 ± 0.107
0.0CysXaa: 0.0 ± 0.0
Asp
7.437AspAla: 7.437 ± 0.886
0.547AspCys: 0.547 ± 0.156
7.874AspAsp: 7.874 ± 1.094
5.741AspGlu: 5.741 ± 0.96
1.203AspPhe: 1.203 ± 0.241
7.929AspGly: 7.929 ± 0.676
1.476AspHis: 1.476 ± 0.301
1.422AspIle: 1.422 ± 0.313
2.023AspLys: 2.023 ± 0.354
6.507AspLeu: 6.507 ± 0.777
1.148AspMet: 1.148 ± 0.272
1.422AspAsn: 1.422 ± 0.286
5.741AspPro: 5.741 ± 0.655
3.336AspGln: 3.336 ± 0.456
5.741AspArg: 5.741 ± 0.666
3.281AspSer: 3.281 ± 0.473
4.21AspThr: 4.21 ± 0.545
4.593AspVal: 4.593 ± 0.523
2.023AspTrp: 2.023 ± 0.356
1.64AspTyr: 1.64 ± 0.356
0.0AspXaa: 0.0 ± 0.0
Glu
7.163GluAla: 7.163 ± 0.654
0.492GluCys: 0.492 ± 0.17
2.953GluAsp: 2.953 ± 0.568
1.258GluGlu: 1.258 ± 0.292
1.969GluPhe: 1.969 ± 0.341
3.718GluGly: 3.718 ± 0.574
1.64GluHis: 1.64 ± 0.344
2.898GluIle: 2.898 ± 0.439
1.094GluLys: 1.094 ± 0.237
4.921GluLeu: 4.921 ± 0.646
1.258GluMet: 1.258 ± 0.259
1.531GluAsn: 1.531 ± 0.252
3.39GluPro: 3.39 ± 0.507
3.992GluGln: 3.992 ± 0.59
3.937GluArg: 3.937 ± 0.646
2.953GluSer: 2.953 ± 0.39
2.625GluThr: 2.625 ± 0.36
4.374GluVal: 4.374 ± 0.534
1.75GluTrp: 1.75 ± 0.421
2.242GluTyr: 2.242 ± 0.47
0.0GluXaa: 0.0 ± 0.0
Phe
3.171PheAla: 3.171 ± 0.51
0.437PheCys: 0.437 ± 0.185
2.187PheAsp: 2.187 ± 0.38
1.367PheGlu: 1.367 ± 0.288
0.93PhePhe: 0.93 ± 0.378
2.789PheGly: 2.789 ± 0.365
0.328PheHis: 0.328 ± 0.147
0.766PheIle: 0.766 ± 0.197
0.711PheLys: 0.711 ± 0.429
1.476PheLeu: 1.476 ± 0.273
0.328PheMet: 0.328 ± 0.13
0.601PheAsn: 0.601 ± 0.257
1.258PhePro: 1.258 ± 0.293
0.82PheGln: 0.82 ± 0.21
1.367PheArg: 1.367 ± 0.321
1.422PheSer: 1.422 ± 0.326
2.351PheThr: 2.351 ± 0.458
1.64PheVal: 1.64 ± 0.324
0.711PheTrp: 0.711 ± 0.192
0.383PheTyr: 0.383 ± 0.15
0.0PheXaa: 0.0 ± 0.0
Gly
9.514GlyAla: 9.514 ± 1.143
0.656GlyCys: 0.656 ± 0.193
5.304GlyAsp: 5.304 ± 0.578
5.359GlyGlu: 5.359 ± 0.677
2.133GlyPhe: 2.133 ± 0.434
7.983GlyGly: 7.983 ± 0.805
1.914GlyHis: 1.914 ± 0.356
4.921GlyIle: 4.921 ± 0.725
2.953GlyLys: 2.953 ± 0.484
6.234GlyLeu: 6.234 ± 0.784
1.859GlyMet: 1.859 ± 0.382
3.226GlyAsn: 3.226 ± 0.527
3.39GlyPro: 3.39 ± 0.471
3.39GlyGln: 3.39 ± 0.436
5.906GlyArg: 5.906 ± 0.614
5.96GlySer: 5.96 ± 0.848
6.234GlyThr: 6.234 ± 0.808
6.562GlyVal: 6.562 ± 0.445
2.023GlyTrp: 2.023 ± 0.32
2.734GlyTyr: 2.734 ± 0.475
0.0GlyXaa: 0.0 ± 0.0
His
2.57HisAla: 2.57 ± 0.406
0.109HisCys: 0.109 ± 0.084
1.203HisAsp: 1.203 ± 0.28
1.148HisGlu: 1.148 ± 0.279
0.219HisPhe: 0.219 ± 0.103
1.695HisGly: 1.695 ± 0.342
0.492HisHis: 0.492 ± 0.185
0.656HisIle: 0.656 ± 0.162
0.219HisLys: 0.219 ± 0.106
2.023HisLeu: 2.023 ± 0.348
0.383HisMet: 0.383 ± 0.138
0.273HisAsn: 0.273 ± 0.107
1.64HisPro: 1.64 ± 0.332
0.766HisGln: 0.766 ± 0.206
1.64HisArg: 1.64 ± 0.317
0.766HisSer: 0.766 ± 0.262
1.367HisThr: 1.367 ± 0.282
1.422HisVal: 1.422 ± 0.317
0.437HisTrp: 0.437 ± 0.118
0.383HisTyr: 0.383 ± 0.137
0.0HisXaa: 0.0 ± 0.0
Ile
7.054IleAla: 7.054 ± 0.639
0.273IleCys: 0.273 ± 0.132
3.5IleAsp: 3.5 ± 0.397
3.554IleGlu: 3.554 ± 0.414
0.875IlePhe: 0.875 ± 0.228
4.538IleGly: 4.538 ± 0.74
0.547IleHis: 0.547 ± 0.185
1.859IleIle: 1.859 ± 0.416
1.367IleLys: 1.367 ± 0.279
2.297IleLeu: 2.297 ± 0.374
0.383IleMet: 0.383 ± 0.175
1.039IleAsn: 1.039 ± 0.207
2.625IlePro: 2.625 ± 0.285
0.82IleGln: 0.82 ± 0.319
2.953IleArg: 2.953 ± 0.39
1.969IleSer: 1.969 ± 0.403
3.39IleThr: 3.39 ± 0.376
3.554IleVal: 3.554 ± 0.453
0.766IleTrp: 0.766 ± 0.171
0.984IleTyr: 0.984 ± 0.229
0.0IleXaa: 0.0 ± 0.0
Lys
4.32LysAla: 4.32 ± 0.729
0.273LysCys: 0.273 ± 0.115
1.476LysAsp: 1.476 ± 0.252
0.328LysGlu: 0.328 ± 0.131
0.766LysPhe: 0.766 ± 0.266
2.406LysGly: 2.406 ± 0.458
0.601LysHis: 0.601 ± 0.194
1.969LysIle: 1.969 ± 0.381
0.656LysLys: 0.656 ± 0.197
3.554LysLeu: 3.554 ± 0.461
0.547LysMet: 0.547 ± 0.18
0.437LysAsn: 0.437 ± 0.192
1.64LysPro: 1.64 ± 0.362
0.711LysGln: 0.711 ± 0.237
2.297LysArg: 2.297 ± 0.364
1.531LysSer: 1.531 ± 0.279
1.476LysThr: 1.476 ± 0.292
2.351LysVal: 2.351 ± 0.225
0.383LysTrp: 0.383 ± 0.146
0.766LysTyr: 0.766 ± 0.178
0.0LysXaa: 0.0 ± 0.0
Leu
9.897LeuAla: 9.897 ± 0.795
0.711LeuCys: 0.711 ± 0.235
7.327LeuAsp: 7.327 ± 0.956
3.007LeuGlu: 3.007 ± 0.481
1.75LeuPhe: 1.75 ± 0.351
8.257LeuGly: 8.257 ± 0.8
1.258LeuHis: 1.258 ± 0.355
3.554LeuIle: 3.554 ± 0.39
1.422LeuLys: 1.422 ± 0.219
6.015LeuLeu: 6.015 ± 0.608
1.203LeuMet: 1.203 ± 0.247
2.187LeuAsn: 2.187 ± 0.382
4.921LeuPro: 4.921 ± 0.498
3.007LeuGln: 3.007 ± 0.475
4.484LeuArg: 4.484 ± 0.426
3.171LeuSer: 3.171 ± 0.375
5.687LeuThr: 5.687 ± 0.598
6.835LeuVal: 6.835 ± 0.584
1.476LeuTrp: 1.476 ± 0.306
1.258LeuTyr: 1.258 ± 0.269
0.0LeuXaa: 0.0 ± 0.0
Met
2.461MetAla: 2.461 ± 0.362
0.109MetCys: 0.109 ± 0.085
0.875MetAsp: 0.875 ± 0.178
0.273MetGlu: 0.273 ± 0.116
0.383MetPhe: 0.383 ± 0.163
0.984MetGly: 0.984 ± 0.236
0.164MetHis: 0.164 ± 0.082
1.039MetIle: 1.039 ± 0.246
0.437MetLys: 0.437 ± 0.178
1.586MetLeu: 1.586 ± 0.233
0.383MetMet: 0.383 ± 0.121
0.82MetAsn: 0.82 ± 0.228
1.914MetPro: 1.914 ± 0.276
0.055MetGln: 0.055 ± 0.048
1.531MetArg: 1.531 ± 0.286
1.148MetSer: 1.148 ± 0.258
4.046MetThr: 4.046 ± 0.445
0.82MetVal: 0.82 ± 0.241
0.328MetTrp: 0.328 ± 0.153
0.109MetTyr: 0.109 ± 0.071
0.0MetXaa: 0.0 ± 0.0
Asn
3.281AsnAla: 3.281 ± 0.559
0.328AsnCys: 0.328 ± 0.118
2.351AsnAsp: 2.351 ± 0.393
1.422AsnGlu: 1.422 ± 0.283
0.82AsnPhe: 0.82 ± 0.241
2.789AsnGly: 2.789 ± 0.403
0.219AsnHis: 0.219 ± 0.115
0.437AsnIle: 0.437 ± 0.161
0.766AsnLys: 0.766 ± 0.179
2.078AsnLeu: 2.078 ± 0.342
0.492AsnMet: 0.492 ± 0.206
0.711AsnAsn: 0.711 ± 0.198
2.023AsnPro: 2.023 ± 0.384
0.492AsnGln: 0.492 ± 0.172
2.023AsnArg: 2.023 ± 0.402
1.312AsnSer: 1.312 ± 0.326
2.297AsnThr: 2.297 ± 0.375
2.242AsnVal: 2.242 ± 0.412
0.547AsnTrp: 0.547 ± 0.163
0.547AsnTyr: 0.547 ± 0.176
0.0AsnXaa: 0.0 ± 0.0
Pro
8.311ProAla: 8.311 ± 0.778
0.492ProCys: 0.492 ± 0.152
4.867ProAsp: 4.867 ± 0.495
3.882ProGlu: 3.882 ± 0.524
1.695ProPhe: 1.695 ± 0.328
4.429ProGly: 4.429 ± 0.478
1.258ProHis: 1.258 ± 0.297
3.171ProIle: 3.171 ± 0.371
1.804ProLys: 1.804 ± 0.269
3.336ProLeu: 3.336 ± 0.428
1.094ProMet: 1.094 ± 0.24
1.586ProAsn: 1.586 ± 0.214
3.5ProPro: 3.5 ± 0.532
1.75ProGln: 1.75 ± 0.328
3.281ProArg: 3.281 ± 0.446
2.242ProSer: 2.242 ± 0.3
4.812ProThr: 4.812 ± 0.529
5.14ProVal: 5.14 ± 0.592
1.422ProTrp: 1.422 ± 0.363
1.039ProTyr: 1.039 ± 0.207
0.0ProXaa: 0.0 ± 0.0
Gln
4.921GlnAla: 4.921 ± 0.525
0.383GlnCys: 0.383 ± 0.136
1.422GlnAsp: 1.422 ± 0.237
1.094GlnGlu: 1.094 ± 0.222
1.531GlnPhe: 1.531 ± 0.228
2.57GlnGly: 2.57 ± 0.456
0.93GlnHis: 0.93 ± 0.3
2.406GlnIle: 2.406 ± 0.382
0.984GlnLys: 0.984 ± 0.202
3.664GlnLeu: 3.664 ± 0.51
1.312GlnMet: 1.312 ± 0.254
1.203GlnAsn: 1.203 ± 0.258
2.023GlnPro: 2.023 ± 0.3
2.734GlnGln: 2.734 ± 0.424
2.351GlnArg: 2.351 ± 0.383
1.312GlnSer: 1.312 ± 0.236
2.351GlnThr: 2.351 ± 0.305
2.843GlnVal: 2.843 ± 0.392
0.711GlnTrp: 0.711 ± 0.187
0.93GlnTyr: 0.93 ± 0.222
0.0GlnXaa: 0.0 ± 0.0
Arg
8.366ArgAla: 8.366 ± 0.821
0.656ArgCys: 0.656 ± 0.217
4.593ArgAsp: 4.593 ± 0.698
4.101ArgGlu: 4.101 ± 0.527
1.531ArgPhe: 1.531 ± 0.237
4.101ArgGly: 4.101 ± 0.507
1.476ArgHis: 1.476 ± 0.363
3.336ArgIle: 3.336 ± 0.54
2.406ArgLys: 2.406 ± 0.373
4.921ArgLeu: 4.921 ± 0.592
2.187ArgMet: 2.187 ± 0.278
1.75ArgAsn: 1.75 ± 0.383
3.062ArgPro: 3.062 ± 0.515
2.515ArgGln: 2.515 ± 0.348
7.218ArgArg: 7.218 ± 0.895
3.609ArgSer: 3.609 ± 0.381
4.921ArgThr: 4.921 ± 0.413
5.14ArgVal: 5.14 ± 0.665
1.258ArgTrp: 1.258 ± 0.307
1.75ArgTyr: 1.75 ± 0.333
0.0ArgXaa: 0.0 ± 0.0
Ser
6.179SerAla: 6.179 ± 0.91
0.383SerCys: 0.383 ± 0.16
3.062SerAsp: 3.062 ± 0.37
2.133SerGlu: 2.133 ± 0.423
1.094SerPhe: 1.094 ± 0.233
4.538SerGly: 4.538 ± 0.815
0.766SerHis: 0.766 ± 0.271
2.57SerIle: 2.57 ± 0.303
1.804SerLys: 1.804 ± 0.334
4.648SerLeu: 4.648 ± 0.565
1.039SerMet: 1.039 ± 0.238
1.422SerAsn: 1.422 ± 0.316
2.953SerPro: 2.953 ± 0.387
1.859SerGln: 1.859 ± 0.335
3.007SerArg: 3.007 ± 0.476
3.007SerSer: 3.007 ± 0.45
3.554SerThr: 3.554 ± 0.631
3.117SerVal: 3.117 ± 0.483
1.312SerTrp: 1.312 ± 0.24
0.93SerTyr: 0.93 ± 0.271
0.0SerXaa: 0.0 ± 0.0
Thr
8.476ThrAla: 8.476 ± 0.684
0.766ThrCys: 0.766 ± 0.235
5.851ThrAsp: 5.851 ± 0.645
4.374ThrGlu: 4.374 ± 0.442
1.914ThrPhe: 1.914 ± 0.362
7.218ThrGly: 7.218 ± 0.662
1.258ThrHis: 1.258 ± 0.283
3.554ThrIle: 3.554 ± 0.494
1.804ThrLys: 1.804 ± 0.332
5.359ThrLeu: 5.359 ± 0.421
1.695ThrMet: 1.695 ± 0.27
2.515ThrAsn: 2.515 ± 0.346
5.523ThrPro: 5.523 ± 0.616
1.531ThrGln: 1.531 ± 0.218
4.156ThrArg: 4.156 ± 0.595
3.445ThrSer: 3.445 ± 0.576
5.741ThrThr: 5.741 ± 0.942
5.413ThrVal: 5.413 ± 0.829
0.875ThrTrp: 0.875 ± 0.187
1.312ThrTyr: 1.312 ± 0.227
0.0ThrXaa: 0.0 ± 0.0
Val
8.257ValAla: 8.257 ± 0.718
0.328ValCys: 0.328 ± 0.159
6.835ValAsp: 6.835 ± 0.799
5.577ValGlu: 5.577 ± 0.57
1.969ValPhe: 1.969 ± 0.367
5.687ValGly: 5.687 ± 0.728
1.695ValHis: 1.695 ± 0.385
2.351ValIle: 2.351 ± 0.361
2.351ValLys: 2.351 ± 0.371
5.031ValLeu: 5.031 ± 0.45
1.203ValMet: 1.203 ± 0.279
1.804ValAsn: 1.804 ± 0.342
4.265ValPro: 4.265 ± 0.488
2.898ValGln: 2.898 ± 0.433
4.593ValArg: 4.593 ± 0.435
3.062ValSer: 3.062 ± 0.475
6.562ValThr: 6.562 ± 0.589
4.648ValVal: 4.648 ± 0.56
1.203ValTrp: 1.203 ± 0.233
2.023ValTyr: 2.023 ± 0.328
0.0ValXaa: 0.0 ± 0.0
Trp
2.953TrpAla: 2.953 ± 0.38
0.383TrpCys: 0.383 ± 0.144
1.148TrpAsp: 1.148 ± 0.224
0.601TrpGlu: 0.601 ± 0.215
0.82TrpPhe: 0.82 ± 0.231
1.203TrpGly: 1.203 ± 0.397
0.437TrpHis: 0.437 ± 0.214
0.547TrpIle: 0.547 ± 0.166
0.547TrpLys: 0.547 ± 0.144
2.351TrpLeu: 2.351 ± 0.374
0.383TrpMet: 0.383 ± 0.118
0.656TrpAsn: 0.656 ± 0.262
1.64TrpPro: 1.64 ± 0.307
1.422TrpGln: 1.422 ± 0.252
1.476TrpArg: 1.476 ± 0.257
0.984TrpSer: 0.984 ± 0.285
1.312TrpThr: 1.312 ± 0.282
1.258TrpVal: 1.258 ± 0.257
0.601TrpTrp: 0.601 ± 0.174
0.328TrpTyr: 0.328 ± 0.133
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.625TyrAla: 2.625 ± 0.57
0.219TyrCys: 0.219 ± 0.129
2.023TyrAsp: 2.023 ± 0.313
1.312TyrGlu: 1.312 ± 0.284
0.328TyrPhe: 0.328 ± 0.113
1.914TyrGly: 1.914 ± 0.328
0.219TyrHis: 0.219 ± 0.102
0.656TyrIle: 0.656 ± 0.202
0.328TyrLys: 0.328 ± 0.137
1.859TyrLeu: 1.859 ± 0.431
0.383TyrMet: 0.383 ± 0.133
0.601TyrAsn: 0.601 ± 0.214
1.367TyrPro: 1.367 ± 0.25
0.601TyrGln: 0.601 ± 0.204
2.789TyrArg: 2.789 ± 0.499
1.422TyrSer: 1.422 ± 0.337
1.312TyrThr: 1.312 ± 0.281
1.476TyrVal: 1.476 ± 0.295
0.547TyrTrp: 0.547 ± 0.189
0.492TyrTyr: 0.492 ± 0.219
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 86 proteins (18289 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski