Trinickia fusca

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Trinickia

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5256 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A494XE96|A0A494XE96_9BURK Type II secretion system protein OS=Trinickia fusca OX=2419777 GN=D7S89_17740 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 10.04FAARR6 pKa = 11.84LLKK9 pKa = 10.78YY10 pKa = 9.92FAARR14 pKa = 11.84PAPRR18 pKa = 11.84VTAVAPAPLLLALEE32 pKa = 4.11PRR34 pKa = 11.84IVYY37 pKa = 9.16DD38 pKa = 3.56ASVAAVGAAARR49 pKa = 11.84HH50 pKa = 5.03HH51 pKa = 6.69VEE53 pKa = 4.16PGADD57 pKa = 3.03AAHH60 pKa = 7.31RR61 pKa = 11.84IGEE64 pKa = 4.32AVAHH68 pKa = 6.11GRR70 pKa = 11.84PAQNDD75 pKa = 3.75SVTPANGGADD85 pKa = 2.89RR86 pKa = 11.84HH87 pKa = 5.69TNQANPAQRR96 pKa = 11.84NVMADD101 pKa = 3.3AVQGSTQVVFVDD113 pKa = 4.05PAVTGYY119 pKa = 7.94QTLLAGLPAGTQVVMLNANTDD140 pKa = 3.09GFAQIAQYY148 pKa = 10.01LQQHH152 pKa = 6.62PGVSAIHH159 pKa = 6.18LLSHH163 pKa = 6.4GAEE166 pKa = 4.14GDD168 pKa = 3.94VQAGSAWLDD177 pKa = 3.15PANFSKK183 pKa = 11.11YY184 pKa = 9.54SAEE187 pKa = 3.94LAQIGAAMQPGGDD200 pKa = 3.81FLIYY204 pKa = 10.54GCDD207 pKa = 3.36VAEE210 pKa = 4.56GADD213 pKa = 3.54GRR215 pKa = 11.84LLVQEE220 pKa = 4.68IAAATHH226 pKa = 6.24LNVAASTDD234 pKa = 3.67LTGAASLGGNWTLEE248 pKa = 4.1YY249 pKa = 10.57QVGNVQTPVIFSAAAEE265 pKa = 4.04QQYY268 pKa = 11.21GSLLSEE274 pKa = 4.42TIEE277 pKa = 5.11DD278 pKa = 3.95YY279 pKa = 11.1TSAAASSLNTGDD291 pKa = 3.45VTSFSLDD298 pKa = 3.64GITYY302 pKa = 8.08TYY304 pKa = 11.23NVANATTVGPDD315 pKa = 3.3SNLQSLPDD323 pKa = 3.7EE324 pKa = 4.82NGSSEE329 pKa = 4.42SLVVNQDD336 pKa = 3.0GVSGLTSITITMQDD350 pKa = 2.51GKK352 pKa = 10.56AFRR355 pKa = 11.84MSSFDD360 pKa = 3.29IDD362 pKa = 3.22IVTNTVATIEE372 pKa = 4.5ANGSTLNDD380 pKa = 3.01ITITSNGVFQTEE392 pKa = 4.53TVDD395 pKa = 3.57LTGDD399 pKa = 3.56SAFDD403 pKa = 3.46VTNSITITFQSNSIVNLGHH422 pKa = 7.55LVYY425 pKa = 10.73QEE427 pKa = 3.8VTPPVVTATGGATTFTSVDD446 pKa = 3.3SSTATPVAVDD456 pKa = 3.2SGVTVSDD463 pKa = 3.99TEE465 pKa = 4.4SGTMSSATVAITGNFHH481 pKa = 7.17SGQDD485 pKa = 3.39VLAFTNTSSVIYY497 pKa = 9.99GSISGSYY504 pKa = 9.73NATTGVLTLSGAGTTAQYY522 pKa = 10.5QAALEE527 pKa = 4.17AVTYY531 pKa = 10.51LDD533 pKa = 3.63TAGTPNTSARR543 pKa = 11.84TISFSVNDD551 pKa = 5.7GIASSTVVTKK561 pKa = 10.15TVDD564 pKa = 3.04VDD566 pKa = 3.91SPPVVTTTGGTTNYY580 pKa = 10.51YY581 pKa = 10.77GGTSATAVDD590 pKa = 4.04SGVSVTDD597 pKa = 4.08ASQGTQASGTVTITSGYY614 pKa = 10.51DD615 pKa = 3.16SSNDD619 pKa = 3.04TLAFTNTSLTLFGNISASFNSSTGVLTLSSSGSTATDD656 pKa = 3.53AQWANAFEE664 pKa = 4.63AVMFSSASTTYY675 pKa = 10.93GNRR678 pKa = 11.84TISFVVNDD686 pKa = 3.93GTDD689 pKa = 3.15NSVAATKK696 pKa = 10.28IVDD699 pKa = 3.82VLNPSPVVTADD710 pKa = 3.09SGSAAFTAGDD720 pKa = 3.87NTTSTPVAIDD730 pKa = 3.14SGLTVTDD737 pKa = 4.62GNSSTLASATIAITGNFHH755 pKa = 7.25SGQDD759 pKa = 3.48VLAFTNNGSTMGNIAGAYY777 pKa = 7.7NATTGVLTLTSAGGIATLAQWQAALDD803 pKa = 3.76AVTYY807 pKa = 9.17TDD809 pKa = 4.21TAITPNNATRR819 pKa = 11.84TISFTASDD827 pKa = 4.24GVNTSNTATRR837 pKa = 11.84TVTVADD843 pKa = 3.69VDD845 pKa = 3.57QTPIVTTTGGTTNYY859 pKa = 10.29VGGASATTVDD869 pKa = 3.56GGVTVSDD876 pKa = 4.91LDD878 pKa = 3.79NATQASGTVSIGSGFHH894 pKa = 7.01SGDD897 pKa = 3.19TLSFTNTSLTLFGNISASYY916 pKa = 10.18NSGTGVLTLSSSGATATDD934 pKa = 3.77AQWANAFDD942 pKa = 4.16AVTFSSTSTTYY953 pKa = 11.28GNRR956 pKa = 11.84TVSFVVNDD964 pKa = 3.78GTEE967 pKa = 3.73NSAAATKK974 pKa = 9.63TVDD977 pKa = 3.84VINPNPVVTTDD988 pKa = 3.04SGSAAFTAGDD998 pKa = 3.87NTTSTPVVIDD1008 pKa = 3.29SGLTVTDD1015 pKa = 4.62GNSSTLASATIAITGNFHH1033 pKa = 7.25SGQDD1037 pKa = 3.49VLAFTNDD1044 pKa = 2.85GSTMGNIAGAYY1055 pKa = 7.7NATTGVLTLTSAGGIATLAQWQAALDD1081 pKa = 4.0SVTYY1085 pKa = 9.91TDD1087 pKa = 4.2TAVTPNNATRR1097 pKa = 11.84TISFTASDD1105 pKa = 4.22GVNTSSTATRR1115 pKa = 11.84TVTVADD1121 pKa = 3.69VDD1123 pKa = 3.57QTPIVTTTGGTTTYY1137 pKa = 9.91TAGASATTVDD1147 pKa = 3.56GGVTVSDD1154 pKa = 5.02LDD1156 pKa = 3.66NTTQASGTVTIGSGFHH1172 pKa = 6.89SGDD1175 pKa = 3.19TLSFTNTSLTLFGNIVAVYY1194 pKa = 10.14NSGTGVLTLSSSGASATDD1212 pKa = 3.68AQWANAFDD1220 pKa = 4.26AVTFSAGASATTGNRR1235 pKa = 11.84TISFVVNDD1243 pKa = 3.9GTEE1246 pKa = 3.73NSAAATKK1253 pKa = 9.67TVDD1256 pKa = 3.31VLGPPTVTTDD1266 pKa = 2.89SGSAAFTAGDD1276 pKa = 3.9NVTSTPVTIDD1286 pKa = 2.77SGLTVTDD1293 pKa = 5.04GSSSTLASATIAITGNFHH1311 pKa = 7.25SGQDD1315 pKa = 3.49VLAFTNDD1322 pKa = 2.85GSTMGNIAGAYY1333 pKa = 7.7NATTGVLTLTSAGGIATLAQWQAALDD1359 pKa = 3.76AVTYY1363 pKa = 9.81TDD1365 pKa = 3.91TAVTPNNATRR1375 pKa = 11.84TISFTTNDD1383 pKa = 3.57GVNTSNTATRR1393 pKa = 11.84TVTVADD1399 pKa = 3.69VDD1401 pKa = 3.57QTPIVTTTGGTTNYY1415 pKa = 10.33VGGTSATAVDD1425 pKa = 3.91GGVTVSDD1432 pKa = 4.34RR1433 pKa = 11.84DD1434 pKa = 3.56NTTQSSGTVSIGSGFHH1450 pKa = 7.01SGDD1453 pKa = 3.19TLSFTNTSSLLFGNIVASYY1472 pKa = 10.05NAGTGVLTLSSSGASATDD1490 pKa = 3.68AQWANAFDD1498 pKa = 4.26AVTFSAGASATTGNRR1513 pKa = 11.84TISFVVNDD1521 pKa = 3.9GTEE1524 pKa = 3.73NSAAATKK1531 pKa = 9.67TVDD1534 pKa = 3.31VLGPPTVTTDD1544 pKa = 2.89SGSAAFTAGDD1554 pKa = 3.9NVTSTPVVIDD1564 pKa = 3.29SGLTVTDD1571 pKa = 5.15GSSSTT1576 pKa = 3.75

Molecular weight: 157.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A494XJQ5|A0A494XJQ5_9BURK Gluconokinase OS=Trinickia fusca OX=2419777 GN=D7S89_07835 PE=3 SV=1
MM1 pKa = 7.65LGGSAWPRR9 pKa = 11.84LAWLRR14 pKa = 11.84LAWLRR19 pKa = 11.84LVWLRR24 pKa = 11.84LVWLRR29 pKa = 11.84LVWLRR34 pKa = 11.84LVWLRR39 pKa = 11.84LVWLRR44 pKa = 11.84LVWLRR49 pKa = 11.84LVWLRR54 pKa = 11.84LAWPRR59 pKa = 11.84LAWPRR64 pKa = 11.84LAWPRR69 pKa = 11.84LAWLRR74 pKa = 11.84LARR77 pKa = 11.84PPLASHH83 pKa = 4.4QTVRR87 pKa = 11.84RR88 pKa = 11.84PLPSPHH94 pKa = 7.06PAWHH98 pKa = 6.59HH99 pKa = 5.97PPRR102 pKa = 11.84CPRR105 pKa = 11.84QRR107 pKa = 11.84LQPRR111 pKa = 11.84HH112 pKa = 5.18

Molecular weight: 13.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5256

0

5256

1758797

24

12197

334.6

36.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.914 ± 0.049

0.925 ± 0.013

5.44 ± 0.028

5.158 ± 0.044

3.552 ± 0.025

8.109 ± 0.053

2.367 ± 0.016

4.48 ± 0.024

2.911 ± 0.028

10.166 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.247 ± 0.017

2.874 ± 0.043

5.06 ± 0.031

3.733 ± 0.027

6.887 ± 0.052

5.825 ± 0.037

5.756 ± 0.059

7.729 ± 0.025

1.359 ± 0.014

2.508 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski