Streptococcus phage Javan399

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A9V1|A0A4D6A9V1_9CAUD HTH cro/C1-type domain-containing protein OS=Streptococcus phage Javan399 OX=2548148 GN=Javan399_0045 PE=4 SV=1
MM1 pKa = 7.47IKK3 pKa = 10.1FRR5 pKa = 11.84AWDD8 pKa = 3.37KK9 pKa = 10.48DD10 pKa = 3.61AKK12 pKa = 10.69VMIYY16 pKa = 10.03HH17 pKa = 7.02IEE19 pKa = 3.85YY20 pKa = 9.02TYY22 pKa = 11.43DD23 pKa = 3.34GLGDD27 pKa = 3.63FQEE30 pKa = 4.48NEE32 pKa = 4.07NIEE35 pKa = 4.61DD36 pKa = 3.87YY37 pKa = 11.47VRR39 pKa = 11.84DD40 pKa = 3.44ISFDD44 pKa = 3.81CFVDD48 pKa = 3.19WLEE51 pKa = 4.29NEE53 pKa = 4.37QFVIMQSTGLFDD65 pKa = 4.16NNGVEE70 pKa = 4.28VFEE73 pKa = 5.04GDD75 pKa = 2.88ILYY78 pKa = 10.67YY79 pKa = 10.15PEE81 pKa = 4.4QDD83 pKa = 3.16EE84 pKa = 5.14DD85 pKa = 3.87NYY87 pKa = 11.52GYY89 pKa = 10.26IEE91 pKa = 4.15FDD93 pKa = 3.18KK94 pKa = 11.31DD95 pKa = 2.94RR96 pKa = 11.84LAFVLDD102 pKa = 3.41NGFEE106 pKa = 3.98KK107 pKa = 10.38FVYY110 pKa = 10.28GEE112 pKa = 3.98YY113 pKa = 10.74GMSYY117 pKa = 9.91IVGNIYY123 pKa = 10.22EE124 pKa = 4.33NPEE127 pKa = 4.42LIEE130 pKa = 4.34SAEE133 pKa = 4.12EE134 pKa = 3.79

Molecular weight: 15.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A9S2|A0A4D6A9S2_9CAUD Capsid and scaffold protein OS=Streptococcus phage Javan399 OX=2548148 GN=Javan399_0015 PE=4 SV=1
MM1 pKa = 7.19KK2 pKa = 10.05RR3 pKa = 11.84KK4 pKa = 9.78KK5 pKa = 9.7IHH7 pKa = 6.38LLSFFQLMICGNTDD21 pKa = 2.71TRR23 pKa = 11.84LKK25 pKa = 9.28AQRR28 pKa = 11.84VAWQSLKK35 pKa = 10.32IVPLRR40 pKa = 11.84LAA42 pKa = 4.04

Molecular weight: 4.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

11339

42

1496

206.2

23.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.597 ± 0.6

0.467 ± 0.107

6.703 ± 0.41

6.544 ± 0.428

4.074 ± 0.219

6.588 ± 0.638

1.508 ± 0.144

7.082 ± 0.312

8.493 ± 0.397

8.228 ± 0.326

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.54 ± 0.162

6.703 ± 0.292

2.69 ± 0.175

4.171 ± 0.228

3.572 ± 0.323

6.544 ± 0.333

6.297 ± 0.422

5.926 ± 0.215

1.376 ± 0.094

3.898 ± 0.29
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski